Article
Multidisciplinary Sciences
Baihao Zhang, Alexis Vogelzang, Michio Miyajima, Yuki Sugiura, Yibo Wu, Kenji Chamoto, Rei Nakano, Ryusuke Hatae, Rosemary J. Menzies, Kazuhiro Sonomura, Nozomi Hojo, Taisaku Ogawa, Wakana Kobayashi, Yumi Tsutsui, Sachiko Yamamoto, Mikako Maruya, Seiko Narushima, Keiichiro Suzuki, Hiroshi Sugiya, Kosaku Murakami, Motomu Hashimoto, Hideki Ueno, Takashi Kobayashi, Katsuhiro Ito, Tomoko Hirano, Katsuyuki Shiroguchi, Fumihiko Matsuda, Makoto Suematsu, Tasuku Honjo, Sidonia Fagarasan
Summary: This study reveals that small metabolites like GABA derived from B-lineage cells can influence immune responses by promoting anti-inflammatory macrophages and enhancing anti-tumour responses. The findings suggest that targeting these metabolites may offer a new approach to immunoregulation.
Article
Chemistry, Applied
Luciana Bitencourt de Souza, Sirlene Valerio Tinti, Ilza Maria de Oliveira Sousa, Ilio Montanari Jr, Jose Luiz da Costa, Joao Ernesto de Carvalho, Mary Ann Foglio, Ana Lucia Tasca Gois Ruiz
Summary: This study evaluated the anti-proliferative potential of ethanolic extract and fractions from Mentha aquatica L aerial parts. F2 fraction showed potent and irreversible anti-proliferative effects without inducing cell cycle arrest in human tumor and non-tumor cell lines. However, storage conditions need to be optimized to enhance F2 stability for further research.
NATURAL PRODUCT RESEARCH
(2022)
Article
Food Science & Technology
Christiane Kruse Faeste, Anita Solhaug, Marion Gaborit, Florian Pierre, Dominique Massotte
Summary: Chronic exposure to the mycotoxin deoxynivalenol (DON) can have neurotoxic effects on brain cells, including impacts on glial cells, cell cycle, inflammatory cytokine release, and neuronal morphology.
Article
Environmental Sciences
Pinar Arslan, Begum Yurdakok-Dikmen, Saniye Cevher Ozeren, Ozgur Kuzukiran, Ayhan Filazi
Summary: The study evaluated the cytotoxic effects of florfenicol and erythromycin on primary cell cultures of Unio crassus and Cyprinus carpio. Both antibiotics showed highest cytotoxic effects on mussel digestive gland and carp liver cells, while the effects on mussel mantle cells were the lowest.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Feng Guo, Kejia Kan, Felix Rueckert, Wolfgang Rueckert, Lin Li, Johannes Eberhard, Tobias May, Carsten Sticht, Wilhelm G. Dirks, Christoph Reissfelder, Prama Pallavi, Michael Keese
Summary: The study found that tumor cells isolated from patients can be used to predict their response to chemotherapy. By lentiviral transduction and expansion, the research team successfully generated expandable primary pancreatic cancer cell lines, which not only retained some tumor-specific biological traits, but also showed ongoing proliferative capacity, providing a patient-specific platform for chemotherapy drug screening.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Sylwia K. Krol, Ewa Bebenek, Magdalena Dmoszynska-Graniczka, Adrianna Slawinska-Brych, Stanislaw Boryczka, Andrzej Stepulak
Summary: This study found that synthetic Bet derivatives can inhibit the proliferation of pediatric cancer cells and are more cytotoxic compared to traditional chemotherapy drugs. Furthermore, the combination with cisplatin can enhance anti-cancer effects, suggesting a promising approach for further research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Miltiadis Fiorentzis, Ekaterina A. Sokolenko, Nikolaos E. Bechrakis, Saskia Ting, Kurt W. Schmid, Ali Sak, Martin Stuschke, Berthold Seitz, Utta Berchner-Pfannschmidt
Summary: Uveal melanoma is commonly treated with radiotherapy, thermotherapy, and tumor resection, but electrochemotherapy (ECT) is emerging as a new therapeutic option for local tumor control. This study examines the radiosensitizing effect of ECT with bleomycin on 3D tumor spheroids with primary and radioresistant uveal melanoma cell lines, finding that primary cell lines are more radiosensitive and require lower doses of irradiation and bleomycin compared to cell lines from previously irradiated tumors. ECT shows potential as a therapeutic radiosensitizing option for radioresistant tumors in clinical settings.
Review
Medicine, General & Internal
Kazutaka Nanba, Amy R. Blinder, William E. Rainey
Summary: The human adrenal cortex is a complex endocrine organ that produces steroids, and abnormal steroidogenesis can lead to various diseases. Adrenocortical cell lines are valuable tools for studying the mechanisms controlling steroid synthesis and tumorigenesis.
TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Microbiology
Javiera Ortiz-Severin, Julia I. Tandberg, Hanne C. Winther-Larsen, Francisco P. Chavez, Veronica Cambiazo
Summary: This study established a cell viability assay suitable for high-throughput analysis to investigate the pathogenic mechanisms of Piscirickettsia salmonis, tracking bacterial infection cycle stages and cellular changes using fluorescence microscopy. Infection induced upregulation of pro-inflammatory cytokines and select P. salmonis virulence genes across different cell culture types. These findings suggest that the identified genes could serve as biomarkers of P. salmonis infection and the infection protocol has the potential for high-throughput screenings of novel antimicrobials targeting this important fish intracellular pathogen.
Article
Biochemistry & Molecular Biology
Natalia Busto, Joana Leitao-Castro, Alfonso T. Garcia-Sosa, Francisco Cadete, Carolina S. Marques, Renata Freitas, Anthony J. Burke
Summary: Molecular hybridization approaches have been used to develop a series of novel N-1,2,3-triazole-isatin hybrids, which show promising anti-proliferative activity against tumor cells. The compounds outperformed cis-platin in terms of anti-proliferation, and potential biological targets, including histone acetyltransferase P-300 and acyl-protein thioesterase 2, were identified. These compounds exhibit advantageous pharmacological properties and affect transcriptional regulation in oncological contexts.
RSC MEDICINAL CHEMISTRY
(2022)
Article
Integrative & Complementary Medicine
Ermias Mergia Terefe, Faith A. Okalebo, Solomon Derese, Moses K. Langat, Eduard Mas-Claret, Nada H. Aljarba, Saad Alkahtani, Gaber El-Saber Batiha, Arabinda Ghosh, Eman A. El-Masry, Joseph Muriuki
Summary: Croton macrostachyus is an important plant in traditional African medicine, which has been widely used to treat various diseases. This study aimed to evaluate the anti-HIV activities and cytotoxic effects of extracts and chemical constituents isolated from C. macrostachyus. The results showed that several compounds exhibited inhibitory effects against HIV and support the potential of C. macrostachyus as a source of anti-HIV constituents.
BMC COMPLEMENTARY MEDICINE AND THERAPIES
(2022)
Article
Virology
Connor G. G. Bamford, Lindsay Broadbent, Elihu Aranday-Cortes, Mary McCabe, James McKenna, David G. Courtney, Olivier Touzelet, Ahlam Ali, Grace Roberts, Guillermo Lopez Campos, David Simpson, Conall McCaughey, Derek Fairley, Ken Mills, Ultan F. Power
Summary: This study finds that the evolution of SARS-CoV-2 is restrained in pediatric respiratory epithelial cells but still exhibits significant genetic plasticity shaped by both viral and host cell factors.
Article
Food Science & Technology
Semih Dalkilic, Ismail Korkmaz, Lutfiye Kadioglu Dalkilic, Gokhan Akay, Songul Fidan
Summary: Cancer is a major cause of worldwide deaths, with increasing incidence and low survival rates. Researchers are actively studying new diagnostic methods, candidate drug molecules, and molecular pathways for targeted new drugs. In this study, the cytotoxic and apoptotic effects of Smilax aspera L. root extracts were investigated for the first time, showing significant cytotoxic effects on three different cancer cell lines and inducing apoptosis in one cell line. This highlights the strategic importance of S. aspera in cancer treatment.
Article
Plant Sciences
Pachiappan Perumal, Sundaramoorthy Dhanasundaram, Annamalai Aravinth, Chinnaperumal Kamaraj, Perumal Santhanam, Rajendran Rajaram
Summary: Betulin, a natural compound with potential antioxidant and anticancer properties, was isolated from brown seaweed in this study. It was characterized using various analytical techniques and shown to exhibit cytotoxicity against several cancer cell lines.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)
Article
Multidisciplinary Sciences
Lloyd Bod, Yoon-Chul Kye, Jingwen Shi, Elena Torlai Triglia, Alexandra Schnell, Johannes Fessler, Stephen M. M. Ostrowski, Max Y. Y. Von-Franque, Juhi R. R. Kuchroo, Rocky M. M. Barilla, Sarah Zaghouani, Elena Christian, Toni Marie Delorey, Kanishka Mohib, Sheng Xiao, Nadine Slingerland, Christopher J. J. Giuliano, Orr Ashenberg, Zhaorong Li, David M. M. Rothstein, David E. E. Fisher, Orit Rozenblatt-Rosen, Arlene H. H. Sharpe, Francisco J. J. Quintana, Lionel Apetoh, Aviv Regev, Vijay K. K. Kuchroo
Summary: In this study, a subset of B cells that expands specifically in the draining lymph node over time in tumour-bearing mice was identified. These B cells express TIM-1 and multiple co-inhibitory molecules and can enhance effector T cell responses to inhibit tumour growth.
Article
Biochemistry & Molecular Biology
Marta Kinga Lemieszek, Iwona Komaniecka, Michal Chojnacki, Adam Choma, Wojciech Rzeski
Summary: The water extract from young green barley (YGB) has been found to have a beneficial impact on natural killer (NK) cells' ability to recognize and eliminate human colon cancer cells. This extract also exhibits a direct anticancer effect. The mixture of oligosaccharides found in the extract consists mainly of glucose and fructose, with a small amount of mannose and galactose.
Article
Cell Biology
Anna Wawruszak, Jarogniew Luszczki, Arkadiusz Czerwonka, Estera Okon, Andrzej Stepulak
Summary: The combination therapy with AGK2 and PAX may be considered as a potential therapeutic regimen in the personalized cure of breast carcinoma patients in the future.
Article
Biochemistry & Molecular Biology
Anna Wawruszak, Jarogniew Luszczki, Estera Okon, Arkadiusz Czerwonka, Andrzej Stepulak
Summary: This study investigates the interaction between the cytotoxic drug PAX and sirtuin inhibitor CAM commonly used in TNBC therapy. Through detailed pharmacodynamic analysis, it is found that their combination can enhance the anti-proliferative and pro-apoptotic effects, suggesting that combination chemotherapy may be a potential approach for treating TNBC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Danuta Krasowska, Agnieszka Gerkowicz, Paula Wroblewska-Luczka, Aneta Grabarska, Katarzyna Zaluska-Ogryzek, Dorota Krasowska, Jarogniew J. Luszczki
Summary: Amantadine has anti-melanoma properties and can alleviate symptoms of Parkinson's disease. Amantadine combined with CDDP showed additive interaction in some melanoma cell lines, while its combination with MTO exhibited synergistic interaction in one cell line.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Paula Wroblewska-Luczka, Justyna Cabaj, Weronika Bak, Julia Bargiel, Aneta Grabarska, Agnieszka Goralczyk, Jarogniew J. Luszczki
Summary: Betulinic acid significantly inhibits the growth of melanoma cell lines in vitro and shows no cytotoxic effect on normal human keratinocytes. Combination therapy of betulinic acid with taxanes generates desirable drug-drug interactions, enhancing the therapeutic effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Sylwia Nakonieczna, Aneta Grabarska, Kinga Gawel, Paula Wroblewska-Luczka, Arkadiusz Czerwonka, Andrzej Stepulak, Wirginia Kukula-Koch
Summary: This study investigated the anti-cancer effects of compounds from Coptis chinensis on gastric cancer cells and found that coptisine showed the highest efficacy. The combination of coptisine with cisplatin exhibited synergistic and additive interactions in vitro, and coptisine was safe for developing zebrafish in vivo. Further studies are warranted to explore the detailed mechanisms of these bioactive compounds in inhibiting gastric cancer cell survival.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Grzegorz Adamczuk, Ewelina Humeniuk, Kamila Adamczuk, Aneta Grabarska, Jaroslaw Dudka
Summary: The study aimed to determine the ability of 2,4-DNP to sensitize prostate cancer cells with different metabolic phenotypes to the action of known anthracyclines. The results showed that the simultaneous application of 2,4-DNP with doxorubicin and epirubicin exhibited a synergistic effect in LNCaP prostate cancer cells, leading to increased oxidative stress and DNA damage.
Article
Pharmacology & Pharmacy
Marta Marszalek-Grabska, Izabela Zakrocka, Barbara Budzynska, Sebastian Marciniak, Katarzyna Kaszubska, Marta Kinga Lemieszek, Sylwia Winiarczyk, Jolanta H. Kotlinska, Wojciech Rzeski, Waldemar A. Turski
Summary: This study aimed to investigate the effects of mephedrone on memory processes and the involvement of kynurenic acid (KYNA). The results showed that binge-like mephedrone administration resulted in memory impairments and reduced KYNA levels in the brain. In vitro studies demonstrated no effect of mephedrone on KYNA production or enzyme activity, while exposure to mephedrone in normal human brain cells showed a modest reduction in cell viability and proliferation.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Agnieszka Drozdz, Adrianna Slawinska-Brych, Dominika Kubera, Magdalena Kimsa-Dudek, Joanna Magdalena Gola, Jolanta Adamska, Celina Kruszniewska-Rajs, Arkadiusz Matwijczuk, Dariusz Karcz, Wojciech Dabrowski, Andrzej Stepulak, Mariusz Gagos
Summary: The study demonstrated the synergistic effect of C1 and NTBD with AmB, showing high antifungal potential and minimal toxicity against normal human cells. In vitro experiments showed that these compounds had low cytotoxicity on human renal proximal tubule epithelial cells and exhibited strong antioxidant activity when combined with AmB. These results suggest the potential use of these compounds in the treatment of fungal infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Artur Bocian, Piotr Kedzierawski, Janusz Kopczynski, Olga Wabik, Anna Wawruszak, Michal Kielbus, Paulina Miziak, Andrzej Stepulak
Summary: Triple-negative breast cancers (TNBCs) are a type of invasive carcinoma that lack specific histological characteristics. The prognosis is poor for TNBC patients, with recurrences and deaths occurring within 3-5 years after diagnosis. This study explored the correlation between the Kaiso transcription factor and breast cancer progression using clinical data and tissue samples. The results suggest that high Kaiso protein expression is associated with better overall survival and disease-free survival, as well as premenopausal age, but further studies are needed due to the heterogeneity of TNBC and context-dependent molecular diversity of Kaiso signaling.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Aneta Grabarska, Jarogniew J. Luszczki, Kinga Gawel, Wirginia Kukula-Koch, Malgorzata Juszczak, Adrianna Slawinska-Brych, Grzegorz Adamczuk, Magdalena Dmoszynska-Graniczka, Nataliia Kosheva, Wojciech Rzeski, Andrzej Stepulak
Summary: The study found that magnoflorine has anticancer effects against gastric cancer cells by inhibiting proliferation and inducing apoptosis. Combination treatment with docetaxel produced additional pharmacological interaction. Moreover, there was no observed toxicity of magnoflorine in a zebrafish model. These findings provide a foundation for the potential therapeutic use of magnoflorine in gastric cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Estera Okon, Maryna Koval, Anna Wawruszak, Adrianna Slawinska-Brych, Katarzyna Smolinska, Myroslav Shevera, Andrzej Stepulak, Wirginia Kukula-Koch
Summary: The study investigated the antiproliferative and cytotoxic effects of E-8-O-G on nervous system cancers. Results showed that E-8-O-G inhibits the viability and proliferation of neuroblastoma and glioblastoma cells, suggesting its potential as a natural antitumor compound for nervous system tumors.
Review
Oncology
Paulina Miziak, Marzena Baran, Ewa Blaszczak, Alicja Przybyszewska-Podstawka, Joanna Kalafut, Jolanta Smok-Kalwat, Magdalena Dmoszynska-Graniczka, Michal Kielbus, Andrzej Stepulak
Summary: ER signaling is a critical regulator in breast cancer and other hormone-sensitive cancers. This article reviews the mechanism of ER-dependent downstream signaling in breast cancer and discusses the clinical implications of ER signaling, including potential endocrine therapies. The challenges associated with therapy resistance caused by acquired ER mutations and potential strategies to overcome them are also discussed.
Article
Environmental Sciences
Michal Kowalczyk, Anna Panasiuk-Kowalczyk, Adam Stadnik, Malgorzata Guz, Marek Cybulski, Witold Jeleniewicz, Andrzej Stepulak, Magdalena Kwiatosz-Muc
Summary: This study aimed to investigate the effects of dexmedetomidine on brain injury during cardiac surgery anesthesia. The results showed that dexmedetomidine can increase the concentrations of MMP-12 and MBP, but have no effects on neurocognitive outcomes postoperatively.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
