Article
Cell Biology
Travis A. Witkowski, Bin Li, Jason G. Andersen, Bhavna Kumar, Edmund A. Mroz, James W. Rocco
Summary: Conditional reprogramming is an effective cell culture technique for rejuvenating epithelial cells. Y27632, a compound that inhibits ROCK isoforms, promotes the process by increasing cell adherence, cell cycle progression, and expression of stem cell markers. Knockout experiments showed that ROCK inhibition alone is insufficient for cell propagation, indicating that Y27632 acts through additional mechanisms. The study suggests that ROCKs play a role in epidermal stem cell renewal, but other pathways are also involved.
JOURNAL OF CELL SCIENCE
(2023)
Article
Veterinary Sciences
Michaela Melzer, Janina Burk, Deborah J. J. Guest, Jayesh Dudhia
Summary: The Rho/ROCK inhibitor Y-27632 had no effect on proliferation activity and cell senescence in equine ASC and BMSC, as shown by the study.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Oncology
Oscar Maiques, Bruce Fanshawe, Eva Crosas-Molist, Irene Rodriguez-Hernandez, Alessia Volpe, Gaia Cantelli, Lena Boehme, Jose L. Orgaz, Faraz K. Mardakheh, Victoria Sanz-Moreno, Gilbert O. Fruhwirth
Summary: This study utilized bioinformatic analyses, in vitro validation, and tissue histology to investigate the mechanisms and effects of metastasis-inhibiting drugs in melanoma. The Rho kinase-myosin II pathway was identified as a potential therapeutic target, leading to the proposal of a migrastatic drug development pipeline.
BRITISH JOURNAL OF CANCER
(2021)
Review
Ophthalmology
Erik Ostler, Douglas Rhee, Edward Burney, Yasemin Sozeri
Summary: The discussion on new class of medication for glaucoma treatment and developments in medicine administration for glaucoma suggest potential for improved outcomes and extended duration of medical control for patients, with a possibility of eliminating the need for glaucoma surgery in some cases.
CURRENT OPINION IN OPHTHALMOLOGY
(2021)
Article
Environmental Sciences
Junyan Yan, Yaxin Tang, Xin Zhong, Huarong Huang, Haonan Wei, Yulei Jin, Yanjiang He, Jinqiao Cao, Lifang Jin, Baowei Hu
Summary: Exposure to carbon blacks (CBs) can activate the RhoA/ROCK pathway in mice lungs, leading to the progression of pulmonary fibrosis. However, treatment with ROCK inhibitor Y-27632 can attenuate this activation and inhibit the development of pulmonary fibrosis.
ENVIRONMENTAL TOXICOLOGY
(2021)
Article
Endocrinology & Metabolism
Yi-Hsuan Wei, Shu-Lang Liao, Sen-Hsu Wang, Chia-Chun Wang, Chang-Hao Yang
Summary: Simvastatin and ROCK inhibitor Y-27632 have been shown to inhibit the fibrosis process induced by TGF-beta, potentially through suppressing the RhoA/ROCK/ERK and p38 MAPK signaling pathways.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Medicine, Research & Experimental
Minghui Li, Xing Lyu, James Liao, Victoria P. Werth, Ming-Lin Liu
Summary: This study found that ROCK regulates NETosis by modulating PKCα nuclear translocation through actomyosin cytoskeletal networks in neutrophils. ROCK1 deficiency can ameliorate UVB-induced skin inflammation by attenuating NETosis and NET-associated cytokines.
Article
Biochemistry & Molecular Biology
Apurwa Singhal, Priyanka Dhankani, Kanchan Lata Gupta, Jayashree Mazumder, R. Adithya, Madhu Dikshit, Sachin Kumar
Summary: Neutrophils are early responders of the immune system, but their over-activation can lead to diseases. Targeting aberrant neutrophil infiltration for diseases like acute lung injury is important. The study found that Rho signaling mitigation affects neutrophil migration capacity, highlighting the role of Rho-dependent L-selectin and AKT signaling in regulating neutrophil recruitment to inflamed lung tissue.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Neurosciences
Yutaka Koizumi, Kunio Mizutari, Satoko Kawauchi, Shunichi Sato, Akihiro Shiotani, Seiji Kakehata
Summary: ROCK inhibition with Y-27632 has been shown to significantly increase the number of synapses in cochlear synaptopathy, demonstrating promising clinical applications for the treatment of sensorineural hearing loss caused by this major pathology.
Article
Biology
Guorong Li, Chanyoung Lee, A. Thomas Read, Ke Wang, Jungmin Ha, Megan Kuhn, Iris Navarro, Jenny Cui, Katherine Young, Rahul Gorijavolu, Todd Sulchek, Casey Kopczynski, Sina Farsiu, John Samples, Pratap Challa, C. Ross Ethier, W. Daniel Stamer
Summary: Netarsudil reduces glucocorticoid-induced ocular hypertension by preventing or attenuating pathologies, acting directly on conventional outflow cells. These findings warrant a randomized prospective clinical study to evaluate its superiority in lowering steroid-induced ocular hypertension.
Article
Chemistry, Multidisciplinary
Yuchong Yang, Tanya K. Ronson, Jieyu Zheng, Nozomi Mihara, Jonathan R. Nitschke
Summary: The binding of fluoride to boron centers within metal-organic cages can regulate cage conformations and modulate host-guest binding. Tetrahedral and octahedral cages respond differently to fluoride binding, inhibiting or enhancing the binding of a second guest, respectively. Allosteric regulation between the tetrahedral and octahedral cages is also possible, with guests transferring between the two cages upon binding of fluoride to both.
Article
Cell & Tissue Engineering
Ryo Takagi, Akiko Tanuma-Takahashi, Saeko Akiyama, Wakana Kaneko, Chika Miura, Masayuki Yamato, Tatsuya Shimizu, Akihiro Umezawa
Summary: The study demonstrates the utility of Y-27632 and LN-511-E8 in maintaining the proliferative activity of hESC-derived keratinocytes in serial cultivation, showing potential for fabricating allogeneic cellular products for regenerating stratified epithelial tissues.
REGENERATIVE THERAPY
(2021)
Article
Plant Sciences
Nolan Scheible, Gyeong Mee Yoon, Andrew G. McCubbin
Summary: This study reports the interaction between the pollen-specific calcium-dependent protein kinase PiCDPK1 and the Rho Guanine Dissociation Inhibitor PiRhoGDI1 in Petunia inflata. It is suggested that PiCDPK1 impacts ROP regulation through PiRhoGDI1, potentially playing a role in the rapid recycling of ROP GTPase at the pollen tube tip.
Article
Chemistry, Multidisciplinary
Taslim A. Al-Hilal, Mohammad Anwar Hossain, Ahmed Alobaida, Farzana Alam, Ali Keshavarz, Eva Nozik-Grayck, Kurt R. Stenmark, Nadezhda A. German, Fakhrul Ahsan
Summary: The study developed a hypoxia-activated prodrug of fasudil that selectively suppresses ROCK activity under hypoxic conditions, exhibiting antiproliferative effects on disease-afflicted pulmonary arterial smooth muscle cells and pulmonary arterial endothelial cells. The prodrug has a long plasma half-life and does not affect systemic blood pressure.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Biochemistry & Molecular Biology
Zixing Chen, Wenmeng He, Thomas Chun Ning Leung, Hau Yin Chung
Summary: This study investigated the effect of the ROCK inhibitor Y-27632 on the immortalization and characterization of cultured rat lingual keratinocytes. Results showed that RLKs could be conditionally immortalized in a high-calcium medium without feeder cells, and removal of Y-27632 led to reversible cytoskeletal reorganization and nuclear enlargement. Additionally, proteomic analysis identified 239 differentially expressed proteins and revealed that RLKs expressed taste bud cell markers but had limited response to tastants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Stephen Y. T. Li, Ming Yan, Haiqi Chen, Tito Jesus, Will M. Lee, Xiang Xiao, C. Yan Cheng
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2018)
Article
Cell Biology
Linxi Li, Baiping Mao, Ming Yan, Siwen Wu, Renshan Ge, Qingquan Lian, C. Yan Cheng
CELL DEATH & DISEASE
(2019)
Article
Endocrinology & Metabolism
Ming Yan, Linxi Li, Baiping Mao, Huitao Li, Stephen Y. T. Li, Dolores Mruk, Bruno Silvestrini, Qingquan Lian, Renshan Ge, C. Yan Cheng
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2019)
Article
Toxicology
Bai-Ping Mao, Linxi Li, Ming Yan, Renshan Ge, Qingquan Lian, C. Yan Cheng
TOXICOLOGICAL SCIENCES
(2019)
Article
Reproductive Biology
Siwen Wu, Ming Yan, Linxi Li, Baiping Mao, Chris K. C. Wong, Renshan Ge, Qingquan Lian, C. Yan Cheng
REPRODUCTIVE TOXICOLOGY
(2019)
Review
Biochemistry & Molecular Biology
Siwen Wu, Ming Yan, Renshan Ge, C. Yan Cheng
TRENDS IN MOLECULAR MEDICINE
(2020)
Review
Toxicology
Lingling Wang, Ming Yan, Siwen Wu, Baiping Mao, Chris K. C. Wong, Renshan Ge, Fei Sun, C. Yan Cheng
TOXICOLOGICAL SCIENCES
(2020)
Article
Endocrinology & Metabolism
Lingling Wang, Ming Yan, Huitao Li, Siwen Wu, Renshan Ge, Chris K. C. Wong, Bruno Silvestrini, Fei Sun, C. Yan Cheng
Summary: Adjudin is a nonhormonal male contraceptive that induces reversible male infertility without perturbing hormone levels. It works by disrupting specific junctions in the testis to induce spermatid exfoliation, without affecting germ cell development. Studies suggest that its contraceptive effects are mediated through changes in microtubule associated proteins and signaling proteins.
Article
Reproductive Biology
Lingling Wang, Ming Yan, Siwen Wu, Xiaolong Wu, Tiao Bu, Chris K. C. Wong, Renshan Ge, Fei Sun, C. Yan Cheng
REPRODUCTIVE TOXICOLOGY
(2020)
Article
Plant Sciences
Xiaobing Lin, Xiaofei An, Lingling Wang, Junhan Zhang, Abudusaimi Abuduwaili, Hao Wang, Ming Yan
Summary: The study demonstrated the therapeutic effect of A. fragrans extract on cervicitis induced by Uu infection, and the lead compound AF-p2 showed an anti-infectious effect in MALP-2 stimulated RAW264.7 cells through downregulating the MyD88-NF-kappa B signaling pathway.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Plant Sciences
Decai Fan, Zi Ying, Ying Yang, Qi Qian, Yuanyuan Li, Panjun Wang, Xiaofei An, Ming Yan
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Review
Cell Biology
Lingling Wang, Ming Yan, Chris K. C. Wong, Renshan Ge, Xiaolong Wu, Fei Sun, C. Yan Cheng
Summary: The microtubule cytoskeleton in Sertoli cells plays a crucial role in spermatogenesis, with recent studies starting to reveal the regulatory biomolecules and mechanisms involved in modulating microtubule dynamics. Utilizing toxicant and pharmaceutical models has provided valuable insights into understanding the dynamics of the microtubule cytoskeleton, particularly the role of microtubule-associated proteins (MAPs) in spermatogenesis.
HISTOLOGY AND HISTOPATHOLOGY
(2021)
Article
Endocrinology & Metabolism
Baiping Mao, Linxi Li, Ming Yan, Chris K. C. Wong, Bruno Silvestrini, Chao Li, Renshan Ge, Qingquan Lian, C. Yan Cheng
Article
Andrology
Lin-Xi Li, Si-Wen Wu, Ming Yan, Qing-Quan Lian, Ren-Shan Ge, C. Yan Cheng
ASIAN JOURNAL OF ANDROLOGY
(2019)
Article
Integrative & Complementary Medicine
Zhou Jun-Neng, Yan Ming, Gao Peng, Hou Ji-Qin, Pham Thi-Anh, Wang Hao
CHINESE JOURNAL OF NATURAL MEDICINES
(2018)