Article
Microbiology
Danitza Xiomara Romero-Calle, Francisnei Pedrosa-Silva, Luiz Marcelo Ribeiro Tome, Vagner Fonseca, Raquel Guimaraes Benevides, Leila Thaise Santana de Oliveira Santos, Tulio de Oliveira, Mateus Matiuzzi da Costa, Luiz Carlos Junior Alcantara, Vasco Ariston de Carvalho Azevedo, Bertram Brenig, Thiago M. Venancio, Craig Billington, Aristoteles Goes-Neto
Summary: Despite the use of new treatments, antimicrobial resistance is still increasing, calling for novel strategies. Bacteriophages have the potential to control bacterial pathogens like Salmonella. A Salmonella phage, Wara, was isolated from water samples in the Subae River Basin in Brazil. Its genome was sequenced using Oxford Nanopore Technologies and Illumina HiSeq, and assembly was carried out using Racon and Unicycler. Wara was found to be a member of the Tequintavirus genus and has therapeutic and biocontrol potential against Salmonella in healthcare and agriculture.
Article
Microbiology
Dongyang Gao, Hongyue Ji, Xin Li, Xiquan Ke, Xiangmin Li, Pin Chen, Ping Qian
Summary: In this study, a polyvalent broad-spectrum phage, GSP044, was isolated from pig farm sewage, which showed the ability to simultaneously lyse multiple serotypes of Salmonella and E. coli. GSP044 has a short latent period, high stability, and good tolerance to chloroform. Genome sequencing analysis revealed that GSP044 belongs to the Demerecviridae family, Epseptimavirus genus, and does not contain any lysogenicity-related, virulence-related, or antibiotic resistance-related genes. The initial application of phage GSP044 showed promising results in reducing biofilm formation, degrading mature biofilm, and decreasing the number of colonized S. Enteritidis in vitro and in vivo.
MICROBIOLOGICAL RESEARCH
(2023)
Article
Microbiology
Haojie Ge, Chao Lin, Yanping Xu, Maozhi Hu, Zhengzhong Xu, Shizhong Geng, Xin 'An Jiao, Xiang Chen
Summary: Phage LP31, as a lytic phage, has been identified as an effective control agent for Salmonella and other foodborne bacteria. LP31 possesses good adhesion properties and can remove biofilms formed by Salmonella. These characteristics make LP31 a potential biocontrol agent for preventing the spread of Salmonella.
VETERINARY MICROBIOLOGY
(2022)
Article
Microbiology
Dongyang Gao, Hongyue Ji, Linkang Wang, Xinxin Li, Dayue Hu, Junna Zhao, Shuang Wang, Pan Tao, Xiangmin Li, Ping Qian
Summary: The selection pressure of phage promotes bacterial mutation, resulting in fitness costs. By utilizing knowledge of bacterial receptors used by phages, we designed a phage cocktail consisting of four phages that targeted different receptors on the Salmonella Enteritidis cell surface. We found that this four-phage cocktail could delay the emergence of phage-resistant bacterial mutants compared to single-phage therapy. Furthermore, the mutants resistant to the four-phage cocktail had reduced virulence and increased susceptibility to antibiotics.
MICROBIOLOGY SPECTRUM
(2022)
Article
Food Science & Technology
Zhenzhen Ning, Lingling Zhang, Linlin Cai, Xinglian Xu, Ying Chen, Huhu Wang
Summary: The study investigated the potential of three different Salmonella phages for biofilm removal and found that a phage cocktail can effectively reduce bacterial colonies in the biofilm and disrupt cell structures. The results suggest that this phage cocktail has potential applications for sterilizing Salmonella biofilm during meat processing.
FOOD SCIENCE AND HUMAN WELLNESS
(2023)
Article
Microbiology
Haojie Ge, Kai Zhang, Dan Gu, Xiang Chen, Xin Wang, Guiqin Li, Hongji Zhu, Yingyan Chang, Ge Zhao, Zhiming Pan, Xin'an Jiao, Maozhi Hu
Summary: Deletion of the rfbN gene in Salmonella Typhimurium led to disruption in LPS biosynthesis and prevented phage S55 from lysing the bacterial strain; the RfbN protein requires involvement of multiple structures for proper functioning.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Infectious Diseases
Lukasz Grabowski, Grzegorz Wegrzyn, Alicja Wegrzyn, Magdalena Podlacha
Summary: This study investigated the effects of a phage cocktail on chickens infected with Salmonella and compared it with antibiotics. The findings showed that the phage cocktail was as effective as antibiotics in killing the bacteria and had a higher safety profile for the chickens.
Article
Biochemistry & Molecular Biology
Richard W. Shuai, Jeffrey A. Ruffolo, Jeffrey J. Gray
Summary: In this study, we propose an Immunoglobulin Language Model (IgLM) that generates synthetic antibody libraries using a generative language model. Unlike previous methods, IgLM formulates antibody design based on text-infilling in natural language, which allows for more flexible sequence generation. Trained on a large dataset of protein sequences, IgLM is capable of generating complete antibody sequences from various species and produces infilled complementarity-determining region (CDR) loop libraries with improved developability profiles.
Article
Microbiology
Katarzyna Kosznik-Kwasnicka, Malgorzata Stasilojc, Lukasz Grabowski, Karolina Zdrojewska, Grzegorz Wegrzyn, Alicja Wegrzyn
Summary: In this study, we demonstrated the high efficacy and acceptable safety profiles of phage therapy against Salmonella enterica strains using vB_SenM-2 and vB_Sen-TO17 phages (both alone and in a cocktail). The phages effectively reduced bacterial cell number, bacterial biofilm, and showed better efficacy compared to known antibiotics. The safety of phage therapy was also confirmed using chromogenic tests and viability assays. The in vivo model showed a significant improvement in animal survival. These findings suggest the potential practical use of phage therapy in poultry farming.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Microbiology
Karen Fong, Yu Tong Lu, Thomas Brenner, Justin Falardeau, Siyun Wang
Summary: Prophages play an important role in the evolution of Salmonella and Verotoxin-producing E. coli (VTEC) and have a high level of diversity. Bacteria have developed CRISPR immunity to defend against phages. Analysis of 118 strains of Salmonella and VTEC revealed diverse prophage sequences and the presence of antibiotic resistance genes. The relationship between spacer abundance and prophage abundance remains unclear.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Rocio Barron-Montenegro, Dacil Rivera, Maria Jesus Serrano, Rodrigo Garcia, Diana M. alvarez, Julio Benavides, Fernanda Arredondo, Francisca P. alvarez, Roberto Bastias, Soledad Ruiz, Christopher Hamilton-West, Eduardo Castro-Nallar, Andrea I. Moreno-Switt
Summary: Salmonella spp. is a worldwide foodborne pathogen, and bacteriophages represent an alternative to antimicrobials for mitigating Salmonella infections. In this study, Salmonella Enteritidis and a lytic phage were coevolved in a culture medium for 21 days. The results showed that both bacteria and phage could survive and develop resistance to each other during the coevolution process. The interactions between Salmonella and lytic phages are crucial for the rational use of phages to control this important foodborne pathogen.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Golshan Shakeri, Jens A. Hammerl, Abdollah Jamshidi, Kiarash Ghazvini, Manfred Rohde, Istvan Szabo, Corinna Kehrenberg, Madeleine Plotz, Sophie Kittler
Summary: This study isolated and characterized six bacteriophages that infect food-derived Salmonella isolates, with phage VB_StyS-LmqsSP1 showing the potential for biocontrol by suppressing Salmonella growth and reducing bacterial titer. The phage demonstrated lytic efficacy under conditions prevalent in food production environments, making it a promising candidate for controlling Salmonella contamination on poultry.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Review
Microbiology
Andrew W. L. Rogers, Renee M. Tsolis, Andreas J. Baumler
Summary: A balanced gut microbiota is important for health, with competition and habitat filters influencing microbiota assembly and maintenance of homeostasis. Once the microbiota has matured, competition and habitat filtering can prevent new microbes from colonizing and protect against opportunistic infections. However, pathogens like Salmonella can overcome colonization resistance through virulence factors that trigger intestinal inflammation, disrupting the normal habitat filtering. This insight is relevant for understanding how inflammation can lead to dysbiosis and noncommunicable diseases.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2021)
Article
Biochemistry & Molecular Biology
Lu Liang, Jiaqi Huang, Ketong Cui, Peiyong Li, Wenjian Shi, Fang Lin, Guijuan Hao, Shuhong Sun
Summary: The combination of a non-productive infection Salmonella phage and a lytic phage can effectively inhibit the growth of pandrug-resistant S. Typhimurium and rapidly suppress bacterial proliferation in vivo. Phage combination treatment helps alleviate inflammatory response and promote bacterial clearance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Yuanshang Wang, Xiaoran Wang, Yi Yan, Jia Wang, Youyou Lu, A. M. Abd El-Aty, Xiaohong Wang
Summary: A new technique utilizing phage T156-mediated aggregation of gold nanoparticles was developed for visual and precise detection of Salmonella. The method offers simplicity, speed, and the ability to distinguish between live and dead Salmonella, demonstrating remarkable sensitivity, specificity, and accuracy. It also presents a novel avenue for the rapid detection of other pathogenic bacteria.
ANALYTICA CHIMICA ACTA
(2023)