Article
Engineering, Biomedical
Xiaojuan Zhang, Chuanchuan He, Yan Chen, Chen Chen, Ruicong Yan, Ting Fan, Yongkang Gai, Tan Yang, Yao Lu, Guangya Xiang
Summary: This study developed a novel nanoplatform that co-delivers Fe(OH)3-doped CaO2 nanocomposites and GOx molecules for synergistic chemodynamic therapy and starvation therapy, providing a mutually reinforced modality based on cyclic Fenton/starvation reactions for effective treatment of hypoxic cancers.
Article
Engineering, Biomedical
Fengying Shao, Yafeng Wu, Zhaoyan Tian, Songqin Liu
Summary: By constructing biomimetic nanoreactors disguised as cancer cell membranes, a synergistic cancer starvation therapy and cascade amplified hypoxia activated chemotherapy were achieved, resulting in significant inhibition of tumor growth and improved therapeutic efficacy.
Article
Materials Science, Biomaterials
Qiaolin Chen, Mi Wu, Jinrong Yao, Zhengzhong Shao, Xin Chen
Summary: In this study, Fe3O4/GOx@RSF/zein nanospheres were developed by loading glucose oxidase (GOx) and Fe3O4 nanoparticles into regenerated silk fibroin/zein nanospheres. These nanospheres achieved a therapeutic effect by depleting glucose and generating highly toxic OH free radicals in the acidic tumor microenvironment.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Chemistry, Multidisciplinary
Ping Ji, Tian-Yang Wang, Guo-Feng Luo, Wei-Hai Chen, Xian-Zheng Zhang
Summary: The tumor cell membrane-camouflaged therapeutic system, embedding apyrase and glucose oxidase into nanoparticles, was shown to disrupt tumor cell energy supply by depleting ATP and glucose, leading to efficient tumor cell death.
CHEMICAL COMMUNICATIONS
(2021)
Article
Oncology
Lifeng Zhang, Shenglin Gao, Xiaokai Shi, Yin Chen, Shuzhang Wei, Yuanyuan Mi, Li Zuo, Chunjian Qi
Summary: This study reveals that NUPR1 plays a carcinogenic role in bladder cancer (BLCA) and its expression is significantly higher in BLCA tissues compared to controls. Lentivirus-mediated interference of NUPR1 can delay tumor growth in BLCA by interfering with cell cycle progression and through epithelial-mesenchymal transition (EMT). Macrophages are the most related immune cells associated with the expression of NUPR1 in BLCA.
Article
Chemistry, Multidisciplinary
Yuzhi Xu, Si-Yang Liu, Leli Zeng, Hansu Ma, Yanfei Zhang, Huihui Yang, Yuchen Liu, Shuo Fang, Jing Zhao, Yunsheng Xu, Charles R. Ashby, Yulong He, Zong Dai, Yihang Pan
Summary: Cuproptosis, a copper-dependent form of regulated cell death, offers great opportunities for cancer treatment. In this study, a glucose oxidase (GOx)-engineered nonporous copper(I) 1,2,4-triazolate ([Cu(tz)]) coordination polymer (CP) nanoplatform, denoted as GOx@[Cu(tz)], is developed for starvation-augmented cuproptosis and photodynamic synergistic therapy. In vivo experimental results show its effectiveness in inhibiting tumor growth.
ADVANCED MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Jang-Hyun Choi, So-Yeon Park, Choong-Jae Lee, Jee-Heun Kim, Tae -Young Jang, So -El Jeon, Youngsoo Jun, Jeong-Seok Nam
Summary: Autophagy has both pro-survival and pro-death functions in cancer, depending on the context. In this study, the researchers investigated the expression patterns of SNAREs, a family of proteins involved in autophagy, in colorectal cancer (CRC) tissues. They found that SEC22B, a vesicle SNARE, was highly expressed in tumor tissues, especially in metastatic tissues. Knocking down SEC22B inhibited CRC cell survival and growth, particularly under stressful conditions, and reduced the number of stress-induced autophagic vacuoles. Furthermore, SEC22B knockdown attenuated liver metastasis in a CRC cell xenograft mouse model. This study suggests that SEC22B plays a crucial role in enhancing the aggressiveness of CRC cells and may be a potential therapeutic target.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Yinmin Zhao, Jiahui Liu, Mengting He, Qi Dong, Lei Zhang, Zhigang Xu, Yuejun Kang, Peng Xue
Summary: The construction of a smart therapeutic nanoplatform consisting of TiO2@Pt/GOx (TPG) allows for efficient sonodynamic therapy (SDT) and starvation therapy (ST), which lead to systemic tumor suppression and hypoxia alleviation in the tumor microenvironment. The optimized energy structure of TPG enables rapid generation of singlet oxygen and hydroxyl radicals. The accumulation of reactive oxygen species and glucose depletion facilitated by TPG result in oxidative damage and energy exhaustion of cancer cells, amplified by Pt-catalyzed oxygen self-supply. The combinatorial therapy also triggers immunogenetic cell death and subsequent suppression of distant tumors and metastasis through antitumor immunity.
Article
Nanoscience & Nanotechnology
Monunith Anithkumar, S. Arunima Rajan, Ahmaduddin Khan, Beata Kaczmarek, Marta Michalska-Sionkowska, Krzysztof Lukowicz, Anna Maria Osyczka, Jagriti Gupta, Niroj Kumar Sahu
Summary: Researchers have developed a novel therapeutic nanosystem (GOx@PEG-MnFe2O4 ) for cancer starvation therapy. The system consists of glucose oxidase (GOx) loaded in polyethylene glycol (PEG) modified manganese ferrite (MnFe2O4 ) nanoparticles (NPs). The combination of GOx and NPs can effectively deplete glucose and eliminate hypoxia in an acidic environment.
ACS APPLIED NANO MATERIALS
(2023)
Review
Gastroenterology & Hepatology
Matias Estaras, Antonio Gonzalez
Summary: In solid tumors, slower vasculature development compared to rapid cell proliferation leads to localized hypoxia, where cells adapt and survive through intracellular pathways and cell-to-cell communication mechanisms.
WORLD JOURNAL OF GASTROENTEROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kitti Szoke, Richard Kajtar, Alexandra Gyongyosi, Attila Czompa, Adina Fesus, Eszter Boglarka Lorincz, Ferenc Daniel Petroczi, Pal Herczegh, Istvan Bak, Aniko Borbas, Ilona Bereczki, Istvan Lekli
Summary: Research shows that chemical modification of cannabidiol (CBD) can overcome its low bioavailability and poor water solubility in therapeutic use. In this study, three new CBD derivatives were synthesized and found to have protective effects on cardiomyocytes exposed to oxidative stress and hypoxia/reoxygenation. The newly synthesized derivatives showed similar or better effects than CBD in decreasing cytotoxicity. CBD2 exhibited the strongest protective effect.
Article
Cell Biology
Lingling Wang, Jiashen Sun, Yueyuan Yin, Yanan Sun, Jinyi Ma, Ruimin Zhou, Xinzhong Chang, Ding Li, Zhi Yao, Shanshan Tian, Kai Zhang, Zhe Liu, Zhenyi Ma
Summary: NUPR1 plays a critical role in breast cancer by regulating the transcription of genes involved in autophagy and drug resistance to maintain Tam resistance. Depletion of NUPR1 leads to premature senescence and tumor suppression, while enhanced autophagic flux facilitates the transition from autophagic survival to premature senescence.
CELL DEATH & DISEASE
(2021)
Review
Cell Biology
Yuyao Feng, Ying Chen, Xiaoyong Wu, Junye Chen, Qingyan Zhou, Bao Liu, Liqin Zhang, Cong Yi
Summary: Macroautophagy/autophagy plays a crucial role in cell survival and maintaining cellular energy homeostasis during starvation or energy stress. Further investigation is needed to understand the specific substrates degraded by autophagy during glucose or energy deprivation and the diverse roles and mechanisms during varying durations of energy starvation.
Article
Cell Biology
Ayelen Valko, Sebastian Perez-Pandolfo, Eleonora Sorianello, Andreas Brech, Pablo Wappner, Mariana Melani
Summary: Hypoxia induces autophagy in Drosophila melanogaster, which is essential for adaptation and survival. The process involves a bona fide autophagic response with waves of autophagosome formation and maturation, and is induced cell autonomously in different tissues. Autophagy under hypoxic conditions can be studied using D. melanogaster as a model organism, offering insights into its role in hypoxia-associated pathologies and developmentally regulated processes.
Article
Nanoscience & Nanotechnology
Parbeen Singh, Brian Youden, Yikun Yang, Yongli Chen, Andrew Carrier, Shufen Cui, Ken Oakes, Mark Servos, Runqing Jiang, Xu Zhang
Summary: A simple formulation of glucose-oxidase-coated CuS nanoparticles has shown high effectiveness in melanoma treatment, utilizing a synergistic combination of glucose starvation, photothermal therapy, and advanced chemodynamic therapy enabled by near-infrared irradiation coupled with Fenton-like reactions enhanced by endogenous chloride.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Energy & Fuels
Irma Robles, Fabricio Espejel-Ayala, Guillermo Velasco, Arely Cardenas, Luis A. Godinez
Summary: This study focused on identifying the best conditions and studying the effects of the operational variables on the integral valorization process of used coffee ground. The results showed that the best conditions for oil extraction were a drying time of 50 degrees C/12 h, ethanol as a solvent with a 12:1 solvent ratio (v/v), and 30 min of extraction time, resulting in the extraction of 0.4 g of oil per gram of spent coffee ground. For the preparation of activated carbon, the best conditions were an activating agent ratio of 1 g(AA)/g(S) and a carbonization temperature of 450 degrees C, resulting in a sorption capacity of 29 mg/g. Additionally, the study found that the extracted coffee oil had a high content of free fatty acids, suggesting the need for an additional purification process.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Immunology
Ana Alcaraz-Sanabria, Esther Cabanas Morafraile, Gonzalo Fernandez-Hinojal, Guillermo Velasco, Pedro Perez-Segura, Atanasio Pandiella, Balazs Gyorffy, Alberto Ocana
Summary: This study identified genes coding for selectively expressed surface membrane proteins in K-RAS mutated NSCLC and discovered immune correlates related to this tumor type. These findings could be used to select and optimize current therapies.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Maria Martinez-Garcia, Guillermo Velasco, Estela Pineda, Miguel Gil-Gil, Francesc Alameda, Jaume Capellades, Mari Cruz Martin-Soberon, Israel Lopez-Valero, Elena Tovar Ambel, Palmira Foro, Alvaro Taus, Montserrat Arumi, Aurelio Hernandez-Lain, Juan Manuel Sepulveda-Sanchez
Summary: The addition of crizotinib to standard treatment shows promising efficacy and safety in newly diagnosed patients with glioblastoma, providing a potential therapeutic option for this patient population.
Editorial Material
Cell Biology
Magali Humbert, Silvia Vega-Rubin-de-Celis, Guillermo Velasco, Yongjie Wei
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Oncology
Nicolas A. Fraunhoffer, Analia Meilerman Abuelafia, Martin Bigonnet, Odile Gayet, Julie Roques, Remy Nicolle, Gwen Lomberk, Raul Urrutia, Nelson Dusetti, Juan Iovanna
Summary: This study developed a Master Regulators (MR)-Gradient model based on transcriptomic profiles derived from both PDAC epithelial and microenvironment cells to reveal the transcriptional networks, epigenomic states, and metabolic pathways underlying PDAC heterogeneity. Integration of methylome and metabolome datasets, along with experimental measurements, showed a close association between PDAC prognosis, tumor epithelial cell phenotype, and immunological component. Metabolic analysis indicated a positive correlation between favorable PDAC phenotype and enzymes involved in complex lipid biosynthesis, while an unfavorable phenotype exhibited an OXPHOS independent metabolism centered on the Warburg effect and glutaminolysis. The study also identified key deregulated pathways in PDAC related to epigenetics.
NPJ PRECISION ONCOLOGY
(2022)
Article
Chemistry, Physical
Nathalia Freitas Freire, Paulo Emilio Feuser, Elena Maria Tovar Ambel, Marco Cordani, Ellen De Pieri, Ricardo Andrez Machado-de-Avila, Acacio A. F. Zielinski, Claudia Sayer, Pedro Henrique Hermes de Araujo, Guillermo Velasco Diez, Elaine Cabral Albuquerque, Rosana Lopes Lima Fialho
Summary: THC, CBD, and other natural compounds produced by Cannabis sativa have therapeutic effects on the human body. Full spectrum extracts, containing controlled amounts of different cannabinoids, are being studied for their potential use in treating diseases like cancer. However, cannabinoids have limited bioavailability due to their low solubility in water and moderate stability. Therefore, there is a need to develop novel methods of cannabinoid administration or encapsulation to improve their efficacy.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)
Article
Oncology
Arkaitz Carracedo
Summary: The process of cellular transformation involves the acquisition of key features known as hallmarks of cancer, which are supported by molecular alterations and changes in the microenvironment. Cellular metabolism is a crucial link between a cell and its environment, and metabolic adaptation is an increasingly important research field in cancer biology.
MOLECULAR ONCOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Javier Plou, Pablo S. Valera, Isabel Garcia, David Vila-Liarte, Carlos Renero-Lecuna, Jesus Ruiz-Cabello, Arkaitz Carracedo, Luis M. Liz-Marzan
Summary: During the response to stress, damaged cells release metabolites and secretomes, which can be utilized in anticancer therapies and biomarker predictions. However, monitoring these processes is challenging, leading to the development of noninvasive secretome screening tools. Machine learning methods and microfluidic chips were used to identify secretome variations and classify cell death, allowing for faster implementation of surface-enhanced Raman scattering (SERS) into cell secretome classification.
Article
Cell Biology
Yizeng Yang, Dharmendra Bhargava, Xiao Chen, Taicheng Zhou, Gizem Dursuk, Wenpeng Jiang, Jinshen Wang, Zhen Zong, Sharyn I. Katz, Gwen A. Lomberk, Raul A. Urrutia, Jonathan P. Katz
Summary: In non-transformed human squamous epithelial cells, the decision of whether to arrest or undergo apoptosis in response to stress is mediated by the interaction between p53 and the zinc-finger transcription factor KLF5. The levels of cellular stress determine the specific outcome, with moderate stress leading to cell survival and severe stress resulting in apoptosis.
CELL DEATH & DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Jan Pencik, Cecile Philippe, Michaela Schlederer, Emine Atas, Matteo Pecoraro, Sandra Grund-Groeschke, Wen (Jess) Li, Amanda Tracz, Isabel Heidegger, Sabine Lagger, Karolina Trachtova, Monika Oberhuber, Ellen Heitzer, Osman Aksoy, Heidi A. Neubauer, Bettina Wingelhofer, Anna Orlova, Nadine Witzeneder, Thomas Dillinger, Elisa Redl, Georg Greiner, David D'Andrea, Johnny R. Ostman, Simone Tangermann, Ivana Hermanova, Georg Schaefer, Felix Sternberg, Elena E. Pohl, Christina Sternberg, Adam Varady, Jaqueline Horvath, Dagmar Stoiber, Tim I. Malcolm, Suzanne D. Turner, Eileen E. Parkes, Brigitte Hantusch, Gerda Egger, Stefan Rose-John, Valeria Poli, Suneil Jain, Chris W. D. Armstrong, Gregor Hoermann, Vincent Goffin, Fritz Aberger, Richard Moriggl, Arkaitz Carracedo, Cathal McKinney, Richard D. Kennedy, Helmut Klocker, Michael R. Speicher, Dean G. Tang, Ali A. Moazzami, David M. Heery, Marcus Hacker, Lukas Kenner
Summary: PTEN and STAT3 are frequently co-deleted genes in metastatic prostate cancer (mPCa). STAT3 controls mPCa through the LKB1/pAMPK/mTORC1/CREB signaling pathway, and metformin can inhibit mPCa growth.
Article
Biochemistry & Molecular Biology
Raquel Garcia-Vilchez, Ana M. M. Anazco-Guenkova, Sabine Dietmann, Judith Lopez, Virginia Moron-Calvente, Silvia D'Ambrosi, Paz Nombela, Kepa Zamacola, Isabel Mendizabal, Saioa Garcia-Longarte, Amaia Zabala-Letona, Ianire Astobiza, Sonia Fernandez, Alejandro Paniagua, Borja Miguel-Lopez, Virginie Marchand, Diego Alonso-Lopez, Angelika Merkel, Ignacio Garcia-Tunon, Aitziber Ugalde-Olano, Ana Loizaga-Iriarte, Isabel Lacasa-Viscasillas, Miguel Unda, Mikel Azkargorta, Felix Elortza, Laura Barcena, Monika Gonzalez-Lopez, Ana M. M. Aransay, Tomas Di Domenico, Manuel A. A. Sanchez-Martin, Javier De Las Rivas, Sonia Guil, Yuri Motorin, Mark Helm, Pier Paolo Pandolfi, Arkaitz Carracedo, Sandra Blanco
Summary: New evidence suggests that epitranscriptomic marks play an important role in cancer development, but the role and implications of altered epitranscriptome deposition in prostate cancer are largely unknown. This study demonstrates that the transfer RNA N-7-methylguanosine (m(7)G) transferase METTL1 is highly expressed in prostate tumors and its depletion leads to the loss of m(7)G tRNA methylation and the formation of small non-coding RNAs derived from 5'tRNA fragments. These small RNAs regulate translation control and favor the synthesis of key regulators and immune effectors, impacting tumor growth suppression and response to immunotherapy in prostate cancer models.
Article
Oncology
Esther Cabanas Morafraile, Cristina Saiz-Ladera, Cristina Nieto-Jimenez, Balazs Gyorffy, Adam Nagy, Guillermo Velasco, Pedro Perez-Segura, Alberto Ocana
Summary: This study describes a novel therapeutic strategy for treating colorectal cancer (CRC) patients with BRAF mutations. By identifying upregulated proteins on the surface of tumor cells as treatment targets and observing the transcriptional profile associated with antigen presenting cells, it is suggested that a combination of anti PD(L)1 with other co-inhibitor receptors can be explored for treating BRAF-mutated CRC patients.
Article
Biochemistry & Molecular Biology
Young-In Chi, Davin R. Jensen, Brian C. Smith, Brian F. Volkman, Angela J. Mathison, Gwen Lomberk, Michael T. Zimmermann, Raul Urrutia
Summary: This study investigates the functional significance of various variants of uncertain significance in the EHMT1 protein. By combining traditional tools with computational methods, the researchers were able to classify the variants into different categories based on their structural and dynamic properties. The damaging variants were found to be located near important functional sites. This study improves upon conventional tools for variant interpretation and provides a molecular mechanism for variant dysfunction.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Correction
Medicine, Research & Experimental
Angela M. Araujo, Andrea Abaurrea, Peio Azcoaga, Joanna I. Lopez-Velazco, Sara Manzano, Javier Rodriguez, Ricardo Rezola, Leire Egia-Mendikute, Fatima Valdes-Mora, Juana M. Flores, Liam Jenkins, Laura Pulido, Inaki Osorio-Querejeta, Patricia Fernandez-Nogueira, Nicola Ferrari, Cristina Viera, Natalia Martin-Martin, Alexandar Tzankov, Serenella Eppenberger-Castori, Isabel Alvarez-Lopez, Ander Urruticoechea, Paloma Bragado, Nicholas Coleman, Asis Palazon, Arkaitz Carracedo, David Gallego-Ortega, Fernando Calvo, Clare M. Isacke, Maria M. Caffarel, Charles H. Lawrie
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Medicine, Research & Experimental
Taewoo Kim, Jessica Johnston, Sonia Castillo-Lluva, Francisco J. Cimas, Stephen Hamby, Santiago Gonzalez-Moreno, Pedro Villarejo-Campos, Alison H. Goodall, Guillermo Velasco, Alberto Ocana, Munitta Muthana, Endre Kiss-Toth
Summary: TRIB1 plays a key role in regulating the phenotype and function of tumor-associated macrophages in breast cancer. High expression of TRIB1 is associated with a better response to chemotherapy and improved patient survival. Both overexpression and knockout of myeloid Trib1 promote mouse breast tumor growth, but through different molecular mechanisms.