期刊
AUTOPHAGY
卷 4, 期 1, 页码 61-62出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/auto.4846
关键词
GFP-LC3; genome; chromosome; homozygous; PCR
类别
GFP-LC3 mice are widely used to monitor autophagy. Here, we report chromosomal mapping of the GFP-LC3 transgene in this mouse model. The transgene was located in a very distal. region of chromosome 2, immediately upstream of a putative open reading frame that is thought to be a part of a pseudogene. With this information, we were able to generate a PCR system to easily determine zygosity. Homozygous GFP-LC3 mice (GFP-LC3(tg/tg)) were healthy, fertile and did not show an abnormal phenotype. Thus, GFP-LC3 integration in this model appears not to affect functions of other genes, justifying use of this mouse model.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据