Article
Biochemistry & Molecular Biology
Jie Liu, Ting Yang, Ya-Hong Yuan, Jia-Yao Hu, Li-Bin Lin, Mei-Li Yang, Dong-Zhu Duan, Guo-Wei Gong, Jian Xiao, Xiao-Ling Wang
Summary: Two new diterpenoidal acrocalyenes A and B were discovered from a plant-associated fungus isolated from Sinomenium acutum in the Qinling Mountains. The compounds showed moderate to strong cytotoxicity against human carcinoma cells and significant inhibitory effects on phytopathogenic fungi.
CHEMISTRY & BIODIVERSITY
(2022)
Article
Biochemistry & Molecular Biology
Zili Feng, Feng Wei, Hongjie Feng, Yalin Zhang, Lihong Zhao, Jinglong Zhou, Jiatao Xie, Daohong Jiang, Heqin Zhu
Summary: In this study, the transcriptome of cotton seedlings pretreated with the endophytic fungus Gibellulopsis nigrescens CEF08111 was sequenced to investigate the molecular mechanism governing the defense response in cotton plants against Verticillium wilt. Analysis of differentially expressed genes (DEGs) revealed enrichment in plant-pathogen interaction, MAPK signaling pathway-plant, and plant hormone signal transduction pathways. Genes regulating defense-related pathways, such as CDPK, FLS2, RPM1, and MYC2, were identified and may contribute to cotton resistance against V. dahliae. WGCNA also identified DEGs related to lignin synthesis. A total of 710 candidate genes associated with cotton resistance to Verticillium wilt were identified. These findings provide insight into the molecular mechanism by which the biocontrol fungus CEF08111 enhances cotton resistance to Verticillium wilt.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Lei Zhang, Yajie Wu, Yongang Yu, Yihao Zhang, Feng Wei, Qian-Hao Zhu, Jinglong Zhou, Lihong Zhao, Yalin Zhang, Zili Feng, Hongjie Feng, Jie Sun
Summary: Protein lysine acetylation is an important post-translational modification mechanism in cellular regulation in eukaryotes. Calmodulin (CaM) is a crucial Ca2+ sensor in eukaryotes and plays a key role in plant immunity. This study shows that GhCaM7, an acetylated protein, is a positive regulator of Verticillium dahliae resistance in cotton and Arabidopsis plants.
Article
Plant Sciences
Yongduo Sun, Mengmeng Zhong, Yuanbao Li, Ruihui Zhang, Lei Su, Guixian Xia, Haiyun Wang
Summary: The study identified the involvement of the GhADF6 gene in cotton's response to the fungal pathogen Verticillium dahliae, showing that its down-regulation makes cotton plants tolerant to infection and leads to actin remodelling. This highlights the importance of actin depolymerizing factors in plant defense.
MOLECULAR PLANT PATHOLOGY
(2021)
Article
Microbiology
H. Martin Kramer, Michael F. Seidl, Bart P. H. J. Thomma, David E. Cook
Summary: Analyzing the H3K27me3 and transcription data of the plant-pathogenic fungus Verticillium dahliae grown in three in vitro cultivation media, it was found that many H3K27me3 domains displayed stable profiles across different conditions, while some differentially transcribed genes were associated with local quantitative differences in H3K27me3 ChIP-seq signals. This suggests that while H3K27me3 may play a role in transcriptional variation, it is not a universal regulator of differential transcription in response to environmental cues.
Article
Engineering, Environmental
Jing Zhang, Xinru Yu, Chaojun Zhang, Qiong Zhang, Ying Sun, Heqin Zhu, Canming Tang
Summary: The study showed that pectin lyase can enhance cotton resistance to Verticillium wilt and induce cell apoptosis of the V. dahliae strain Vd080. Pectin lyase affects various cellular processes in the Vd080 strain, leading to ER stress and ultimately cell apoptosis. These findings suggest that pectin lyase could be a potential biocontrol agent for managing Verticillium wilt in cotton.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Microbiology
Ibrahim ElDesouki-Arafat, Hani K. Aldebis-Albunnai, Enrique Vargas-Osuna, Antonio Trapero, Francisco J. Lopez-Escudero
Summary: Experiments conducted in semi-controlled conditions found that Phloeotribus scarabaeoides is not a vector of Verticillium dahliae, the pathogen causing Verticillium wilt of olive trees.
Review
Plant Sciences
Yutao Zhu, Mei Zhao, Taotao Li, Lianzhe Wang, Chunli Liao, Dongxiao Liu, Huamin Zhang, Yanpeng Zhao, Lisen Liu, Xiaoyang Ge, Bingbing Li
Summary: Cotton is a widely grown crop due to its economic value. Verticillium wilt, caused by a pathogen called Verticillium dahliae, is a devastating disease that affects cotton crops, leading to yield losses and lower fiber quality. Developing genetically engineered cotton varieties with resistance to Verticillium wilt is an effective strategy, but there are challenges due to the lack of resistance gene resources. Understanding the molecular mechanisms of the interaction between V. dahliae and cotton is crucial in identifying genes related to disease resistance.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biology
Dan-Dan Zhang, Xiao-Feng Dai, Steven J. Klosterman, Krishna Subbarao, Jie-Yin Chen
Summary: This review systematically discusses the roles of the secretome of Verticillium dahliae in vascular occlusion and modulation of plant defence responses, highlighting its role in inducing Verticillium wilt. By manipulating plant defences and hormone levels, the secretome colludes with plant defence responses to modulate wilting symptoms, bridging historical concepts of both toxin production and vascular occlusion as the cause of Verticillium wilt.
BIOLOGICAL REVIEWS
(2022)
Article
Biotechnology & Applied Microbiology
Ping Qiu, Baoxin Zheng, Hang Yuan, Zhaoguang Yang, Keith Lindsey, Yan Wang, Yuqing Ming, Lin Zhang, Qin Hu, Muhammad Shaban, Jie Kong, Xianlong Zhang, Longfu Zhu
Summary: Verticillium dahliae is a pathogenic fungus that causes diseases in dicot plants, and its virulence is affected by different pathotypes. This study identified an elicitor called VP2, which was highly expressed in the defoliating pathotype V991 and triggered the immune response in cotton, enhancing its resistance to V. dahliae. The overexpression of VP2 also resulted in the upregulation of genes involved in JA, SA, and lignin synthesis, as well as an increase in the contents of these compounds.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Plant Sciences
Mengxi Sun, Zhiqiang Zhang, Zhongying Ren, Xingxing Wang, Wenjie Sun, Hongjie Feng, Junjie Zhao, Fei Zhang, Wei Li, Xiongfeng Ma, Daigang Yang
Summary: The study revealed the mechanism of sugar allocation in cotton plants infected by Verticillium dahliae, with GhSWEET42 playing a key role by mediating glucose translocation during infection. Manipulating the expression of GhSWEET42 can effectively inhibit V. dahliae infection.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biology
Jie-Yin Chen, Dan-Dan Zhang, Jin-Qun Huang, Ran Li, Dan Wang, Jian Song, Krishna D. Puri, Lin Yang, Zhi-Qiang Kong, Bang-Zhuo Tong, Jun-Jiao Li, Yu-Shan Huang, Ivan Simko, Steven J. Klosterman, Xiao-Feng Dai, Krishna V. Subbarao
Summary: In a long-term field experiment with lettuce, the fungal pathogen Verticillium dahliae exhibited nonrandom genetic adaptations at a whole genome scale, accumulating SNPs/In-Dels primarily in hypervariable regions encoding signal transduction and transcriptional regulation components.
Article
Plant Sciences
Jun Yang, Meixia Xie, Xingfen Wang, Guoning Wang, Yan Zhang, Zhikun Li, Zhiying Ma
Summary: The study provides a comprehensive analysis of the cotton WAK gene family, with 28 genes identified to potentially play a role in cotton resistance to Verticillium wilt. Silencing these genes using VIGS technology significantly impacts cotton's ability to resist Verticillium wilt.
Article
Biochemistry & Molecular Biology
Lin Liu, Ya-Duo Zhang, Dan-Dan Zhang, Yuan-Yuan Zhang, Dan Wang, Jian Song, Jian Zhang, Ran Li, Zhi-Qiang Kong, Steven J. Klosterman, Xiao-Feng Dai, Krishna V. Subbarao, Jun Zhao, Jie-Yin Chen
Summary: Verticillium dahliae is a soil-borne plant pathogenic fungus that causes Verticillium wilt on numerous dicotyledonous plant species. Research has shown that MAT1-2 strains of V. dahliae exhibit higher growth rates, melanin production, degradation capabilities, and virulence compared to MAT1-1 strains when subjected to various stimuli. This suggests that MAT1-2 strains have competitive advantages in adapting to their environment and showing higher virulence, potentially explaining their current abundance in agricultural and sylvicultural ecosystems.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jie Wang, Dan Wang, Xiaobin Ji, Jun Wang, Steven J. Klosterman, Xiaofeng Dai, Jieyin Chen, Krishna V. Subbarao, Xiaojuan Hao, Dandan Zhang
Summary: This study shows that the small cysteine-rich protein VdSCP23 from Verticillium dahliae can inhibit cell necrosis, reactive oxygen species burst, electrolyte leakage, and the expression of defense-related genes in Nicotiana benthamiana. Localization analysis reveals that VdSCP23 is mainly located in the plasma membrane and nucleus of plant cells. The inhibition of immune responses by VdSCP23 is not dependent on its nuclear localization or cysteine residues, but on N-glycosylation sites and the integrity of protein structure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
