Review
Biochemistry & Molecular Biology
Zhuo Xie, Zongshan Shen, Peimeng Zhan, Jiayu Yang, Qiting Huang, Shuheng Huang, Lingling Chen, Zhengmei Lin
Summary: Treatment of pulpal and periapical diseases faces challenges, but significant progress has been made in achieving true pulp tissue regeneration through technologies such as stem cells, biomaterials, and growth factors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Dentistry, Oral Surgery & Medicine
Parisa Noohi, Mohammad J. Abdekhodaie, Mohammad H. Nekoofar, Kerstin M. Galler, Paul M. H. Dummer
Summary: This article reviews recent advances in the design and fabrication of scaffolds for the regeneration of pulp-dentine complexes via tissue engineering approaches. Different types of scaffolds and their advantages and limitations are covered. The importance of vascular network establishment for successful pulp-dentine complex regeneration and the corresponding scaffold design strategies are discussed.
INTERNATIONAL ENDODONTIC JOURNAL
(2022)
Review
Biochemistry & Molecular Biology
Ella Ohlsson, Kerstin M. Galler, Matthias Widbiller
Summary: Efforts in tissue engineering to repair damaged dental pulp tissue have shown positive results, but clinical measures cannot distinguish between true regeneration and repair. Therefore, research models are crucial in understanding treatment outcomes and advancing dental pulp regeneration. This review provides information on various in vitro and in vivo models, discusses the differences between monolayer and three-dimensional cell cultures, presents a new in vivo transplantation model for dental pulp regeneration, and introduces different animal models used for in vivo research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dentistry, Oral Surgery & Medicine
Hengameh Bakhtiar, Azin Ashoori, Sarah Rajabi, Mohamad Pezeshki-Modaress, Alireza Ayati, Mohammad Reza Mousavi, Mohammad Reza Ellini, Amir Kamali, Amir Azarpazhooh, Anil Kishen
Summary: The 30 mg/ml HAM ECM scaffold exhibited optimal physical properties and enhanced hDPSC migration. The HAM ECM scaffold did not hinder the formation of pulp-like tissue and revascularization within the root canal when used as both cell-free and cell-loaded scaffold. These findings underscore the potential of HAM ECM membrane for further exploration in regenerative endodontics.
INTERNATIONAL ENDODONTIC JOURNAL
(2022)
Article
Dentistry, Oral Surgery & Medicine
C. Katata, J. Sasaki, A. Li, G. L. Abe, J. E. Nor, M. Hayashi, S. Imazato
Summary: Vascularized DPSC constructs, formed by inducing endothelial differentiation, showed enhanced blood supply and pulp regeneration when transplanted into human pulpless teeth, compared to DPSC constructs without prevascularization. This study demonstrates the potential of vascularized DPSC constructs as a biomaterial for novel dental pulp regeneration.
JOURNAL OF DENTAL RESEARCH
(2021)
Review
Chemistry, Physical
Necdet Adanir, Zohaib Khurshid, Jithendra Ratnayake
Summary: This systematic review evaluates the effectiveness of decellularized dental pulp extracellular matrix (DP-ECM) in pulpal regeneration. Although the results of the studies suggest that DP-ECM may stimulate pulpal regeneration, the presence of bias and methodological deficiencies limit the ability to draw definitive conclusions. Further research, both clinical and pre-clinical, is needed to provide more conclusive evidence.
Article
Biotechnology & Applied Microbiology
Yuanyuan Shi, Yingxin Wang, Zhaochen Shan, Zhenhua Gao
Summary: Decellularized submandibular gland extracellular matrix (DSMG) was found to have similar structure and composition to dental pulp. DSMG could serve as an alternative scaffold for dental pulp regeneration, supporting adhesion and proliferation of dental pulp stem cells. Implanted cell-seeded DSMG formed a vascularized dental pulp-like tissue expressing markers involved in dentinogenesis and angiogenesis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Dentistry, Oral Surgery & Medicine
Jose Luis Alvarez-Vasquez, Cristina Paola Castaneda-Alvarado
Summary: This article highlights the active roles of dental pulp fibroblasts (DPFs) in pulp biology beyond being passive cells, and provides an integrative analysis of their roles and functions.
JOURNAL OF ENDODONTICS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Pedram Iranmanesh, Mahmoud Torabinejad, Masoud Saatchi, Davood Toghraie, Sayed Mohammad Razavi, Abbasali Khademi
Summary: This study aimed to evaluate the effect of the duration of root canal infection on the ability of dentin-pulp complex regeneration. The results showed that long-term root canal infection decreased the ability of the body to regenerate the dentin-pulp complex.
JOURNAL OF ENDODONTICS
(2022)
Article
Materials Science, Multidisciplinary
Al Shehadat Saaid, A. Abou Neel Ensanya, Sheikh Abdul Hamid Suzina, Asma Abdullah Nurul, Abdul Rani Samsudin, Ahmad Azlina
Summary: This study investigated the ability of glycerol or cryopreserved de-epithelialized human amniotic membrane (GAM or CAM) to induce odontogenic differentiation of deciduous teeth stem cells (DTSCs). The results showed that both GAM and CAM supported DTSCs attachment, with higher proliferation observed on CAM. The expression of inflammatory cytokines was also higher on CAM. When DPSCs were transplanted onto GAM, strong cell attachment to the dentin surface was observed.
MATERIALS CHEMISTRY AND PHYSICS
(2022)
Article
Chemistry, Physical
Norah A. AlHowaish, Dina I. AlSudani, Rita Khounganian, Nehal AlMuraikhi
Summary: This study histologically evaluated the outcome of regenerative endodontic procedures (REPs) using Restylane Lyft as a scaffold. The results showed that combining a blood clot with Restylane Lyft as a scaffold can significantly improve vascularization and collagen fiber formation in regenerated soft connective tissues in non-infected, pulpless, immature teeth.
Article
Dentistry, Oral Surgery & Medicine
D. G. Soares, E. A. F. Bordini, E. S. Bronze-Uhle, F. B. Cassiano, I. S. P. Silva, M. O. Gallinari, H. R. Matheus, J. M. Almeida, L. T. A. Cintra, J. Hebling, C. A. de Souza Costa
Summary: This study evaluated the bioactive potential of a simvastatin (SV)-releasing chitosan-calcium-hydroxide (CH-Ca) scaffold for dentin regeneration. Results showed that the CH-Ca scaffold combined with SV could promote odontoblastic differentiation of dental pulp cells (DPCs) in vitro and in vivo, inducing overexpression of odontoblastic markers and intense mineralization. The scaffold also had a chemoattractant effect on DPCs and enhanced mineralized matrix deposition, showing promise for regenerative dentistry applications.
JOURNAL OF DENTAL RESEARCH
(2021)
Article
Biology
Matthias Widbiller, Andreas Rosendahl, Melanie Woelflick, Moritz Linnebank, Benedikt Welzenbach, Karl-Anton Hiller, Wolfgang Buchalla, Kerstin M. Galler
Summary: In this study, a chairside protocol using endogenous dentin matrix proteins for dental pulp regeneration was designed. The effects of disinfectants on growth factors in the root canal were determined to develop a translational protocol for clinical application. It was found that growth factors can be successfully extracted from root canals and used for tissue engineering.
Article
Engineering, Biomedical
Zain Siddiqui, Amanda M. Acevedo-Jake, Alexandra Griffith, Nurten Kadincesme, Kinga Dabek, Dana Hindi, Ka Kyung Kim, Yoshifumi Kobayashi, Emi Shimizu, Vivek Kumar
Summary: Pulp capping and dental pulp regeneration are evolving techniques to regenerate pulp tissue and maintain tooth vitality, with various methods and materials being used in regenerative endodontics.
BIOACTIVE MATERIALS
(2022)
Review
Chemistry, Medicinal
Teerawat Sukpaita, Suwabun Chirachanchai, Atiphan Pimkhaokham, Ruchanee Salingcarnboriboon Ampornaramveth
Summary: This review provides an overview of chitosan-based biomaterials for bone tissue engineering and their applications in treating bone defects. The focus is on the characteristics of chitosan, scaffold fabrication processes, advantages and disadvantages, and future directions in the field.
Article
Materials Science, Biomaterials
A. M. Abd El-Aziz, Rania M. El Backly, Nahla A. Taha, Azza El-Maghraby, Sherif H. Kandil
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2017)
Article
Infectious Diseases
Reem W. Sadek, Sybel M. Moussa, Rania M. El Backly, Abdel Fattah Hammouda
MICROBIAL DRUG RESISTANCE
(2019)
Article
Engineering, Biomedical
Hisham El Nawam, Rania El Backly, Amira Zaky, Amr Abdallah
LASERS IN MEDICAL SCIENCE
(2019)
Article
Materials Science, Ceramics
Suman Saha, Arnab Bhattacharjee, Sk. Hasanur Rahaman, Sayantan Ray, Mona K. Marei, Himanshu Jain, Jui Chakraborty
INTERNATIONAL JOURNAL OF APPLIED GLASS SCIENCE
(2020)
Article
Dentistry, Oral Surgery & Medicine
Noha Mohamed El-Kateb, Rania Noaman El-Backly, Wessam Mohamed Amin, Amr Mohamed Abdalla
JOURNAL OF ENDODONTICS
(2020)
Article
Polymer Science
Ahmed Abd El-Fattah, Mohamad Nageeb Hassan, Ahmad Rashad, Mona Marei, Sherif Kandil
INTERNATIONAL JOURNAL OF POLYMER ANALYSIS AND CHARACTERIZATION
(2020)
Article
Chemistry, Physical
Noha Elshazly, Abdelaziz Khalil, Manal Saad, Marco Patruno, Jui Chakraborty, Mona Marei
Article
Dentistry, Oral Surgery & Medicine
Semha E. Elnaggar, Rania M. El Backly, Ashraf Mamdouh Zaazou, Sonia Morsy Elshabrawy, Amr Ahmed Abdallah
Summary: The study found that increasing NaOCl concentration significantly reduced the mechanical properties of dentin, while the concentrations recommended for regenerative endodontic treatment did not have a significant effect on dentin mechanical properties. However, the use of passive ultrasonic irrigation (PUI) with increased irrigation time may have an effect, even with low NaOCl concentration.
AUSTRALIAN ENDODONTIC JOURNAL
(2021)
Article
Dentistry, Oral Surgery & Medicine
Ahmed Mahmoud, Sybel Moussa, Rania El Backly, Reem El-Gendy
Summary: This study evaluated the indirect effects of residual silver nanoparticles gel on human dental pulp stromal cells (DPSCs). The results showed that dentin discs treated with concentrations of AgNPs gel for 7 days allowed more than 90% DPSCs cell attachment after 24 hours. The cytotoxicity and proliferation of DPSCs in response to AgNPs gel were comparable to those with calcium hydroxide. This suggests that AgNPs gel may be a promising candidate for clinical use in regenerative endodontics.
Article
Dentistry, Oral Surgery & Medicine
Yasmine Elhamouly, Rania M. El Backly, Dalia M. Talaat, Samia S. Omar, Maha El Tantawi, Karin M. L. Dowidar
Summary: This study compared the outcomes of Biodentine and 70S30C-BAG as pulpotomy agents in primary teeth, showing that Biodentine demonstrated better clinical, histologic, and anti-inflammatory outcomes compared to 70S30C-BAG, promoting pulp healing and regeneration. 70S30C-BAG resulted in pulp necrosis due to persistent inflammation, leading to clinical failure.
CLINICAL ORAL INVESTIGATIONS
(2021)
Article
Medicine, General & Internal
Rania M. El Backly, Gehan Sherif Kotry, Hassan Moussa
Summary: Detailed treatment planning and execution are essential for regenerative approaches to retain fused permanent teeth, with long-term follow-up necessary to monitor stability aesthetically and functionally.
CLINICAL CASE REPORTS
(2021)
Article
Dentistry, Oral Surgery & Medicine
M. S. Saad, M. Fata, A. Farouk, A. M. A. Habib, M. Gad, M. B. Tayel, M. K. Marei
JDR CLINICAL & TRANSLATIONAL RESEARCH
(2020)
Review
Biotechnology & Applied Microbiology
Mona K. Marei, Rania M. El Backly
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2018)
Review
Biotechnology & Applied Microbiology
Ola A. Nada, Rania M. El Backly
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2018)