期刊
JOURNAL OF APPLIED TOXICOLOGY
卷 36, 期 7, 页码 886-895出版社
WILEY
DOI: 10.1002/jat.3261
关键词
Synechogobius hasta; lipid deposition; lipid metabolism; Zn; molecular mechanism
类别
资金
- National Natural Science Fund of China [31422056, 31372547, 31072226, 30800850]
- Fundamental Research Funds for the Central Universities, China [2662015PY017, 2014JQ002, 2013PY073]
The present study was conducted to determine the effect and mechanism of waterborne Zn exposure influencing hepatic lipid deposition and metabolism in javelin goby Synechogobius hasta. S. hasta were exposed to four waterborne Zn concentrations (Zn 0.005 [control], 0.18, 0.36 and 0.55 mg l(-1), respectively) for 60 days. Sampling occurred at days 20, 40 and 60, respectively. Zn exposure increased Zn content, declined hepatic lipid content and reduced viscerosomatic and hepatosomatic indices and lipogenic enzyme activities, including 6-phosphogluconate dehydrogenase (6PGD), glucose-6-phosphate dehydrogenase (G6PD), malic enzyme (ME) and fatty acid synthase (FAS). At days 20 and 60, Zn exposure decreased hepatic mRNA levels of 6PGD, G6PD, ME, FAS, acetyl-CoA carboxylase (ACC)alpha, ACC beta, hormone-sensitive lipase (HSL)a, HSLb, sterol-regulator element-binding protein (SREBP)-1, peroxisome proliferators-activated receptor (PPAR)alpha and PPAR gamma. However, the mRNA levels of CPT 1 and adipose triglyceride lipase increased following Zn exposure. On day 40, Zn exposure reduced hepatic mRNA expression of 6PGD, G6PD, ME, FAS, ACC alpha, ACC beta, HSLa, HSLb, SREBP-1 and PPAR gamma but increased mRNA expression of CPT 1, adipose triglyceride lipase and PPAR alpha. General speaking, Zn exposure reduced hepatic lipid content by inhibiting lipogenesis and stimulating lipolysis. For the first time, the present study provided evidence that chronic Zn exposure differentially influenced mRNA expression and activities of genes and enzymes involved in lipogenic and lipolytic metabolism in a duration-dependent manner, and provided new insight into the relationship between metal elements and lipid metabolism. Copyright (C) 2015 John Wiley & Sons, Ltd.
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