期刊
ATMOSPHERIC ENVIRONMENT
卷 42, 期 28, 页码 6767-6774出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.atmosenv.2008.05.018
关键词
Bioaerosols; Epifluorescence; Occupational health; Poultry firm; Q-PCR; Sewage treatment plant
资金
- Swiss National Foundation [3200BO-104246]
Traditional culture-dependent methods to quantify and identify airborne microorganisms are limited by factors such as short-duration sampling times and inability to count non-culturable or non-viable bacteria. Consequently, the quantitative assessment of bioaerosols is often underestimated. Use of the real-time quantitative polymerase chain reaction (Q-PCR) to quantify bacteria in environmental samples presents an alternative method, which should overcome this problem. The aim of this study was to evaluate the performance of a real-time Q-PCR assay as a simple and reliable way to quantify the airborne bacterial load within poultry houses and sewage treatment plants, in comparison with epifluorescence microscopy and culture-dependent methods. The estimates of bacterial load that we obtained from real-time PCR and epifluorescence methods, are comparable, however, our analysis of sewage treatment plants indicate these methods give values 270-290 fold greater than those obtained by the impaction on nutrient agar method. The culture-dependent method of air impaction on nutrient agar was also inadequate in poultry houses, as was the impinger-culture method, which gave a bacterial load estimate 32-fold lower than obtained by Q-PCR. Real-time quantitative PCR thus proves to be a reliable, discerning, and simple method that could be used to estimate airborne bacterial load in a broad variety of other environments expected to carry high numbers of airborne bacteria. (C) 2008 Elsevier Ltd. All rights reserved.
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