期刊
JOURNAL OF APPLIED PHYSIOLOGY
卷 119, 期 4, 页码 374-384出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/japplphysiol.01000.2014
关键词
endurance exercise; exercise adaptation; gene expression; mitochondria; transcription
资金
- Swedish National Center for Research in Sports
- Karolinska Institute Foundation
Individuals with high skeletal muscle mitochondrial content have a lower risk to acquire cardiovascular and metabolic disease, obesity, and type II diabetes. Regular endurance training increases mitochondrial density through a complex network of transcriptional regulators that in an accumulated way are affected by each single exercise bout. The aim of the present study was to investigate the effect of a single exercise bout on the levels of PGC-1 alpha and related regulatory factors important for the initial phase of skeletal muscle adaptation. Ten men and ten women were randomized to either an exercise group (60 min cycling at a work load corresponding to 70% of peak oxygen uptake) or a nonexercising control group. Skeletal muscle biopsies were taken before, at 30 min, and at 2, 6, and 24 h after the intervention. Twenty-two mRNA transcripts and five proteins were measured. With exercise, protein levels of PGC-1 alpha-ex1b increased, and this elevation occurred before that of total PGC-1 alpha protein. We also demonstrated the existence and postexercise expression pattern of two LIPIN-1 (LIPIN-1 alpha and LIPIN-1 beta) and three NCoR1 (NCoR1-1, NCoR1-2, and NCoR1-3) isoforms in human skeletal muscle. The present study contributes new insights into the initial signaling events following a single bout of exercise and emphasizes PGC-1 alpha-ex1b as the most exercise-responsive PGC-1 alpha isoform.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据