Article
Hematology
Alessandro Aliotta, Debora Bertaggia Calderara, Maxime G. Zermatten, Lorenzo Alberio
Summary: The study demonstrated contrasting patterns in the intracellular ion concentrations of COAT-activated platelets compared to non-COAT platelets, with the former showing high and prolonged calcium concentration, transient sodium increase, and fast potassium efflux. Inhibition of the sodium-calcium exchanger (NCX) reduced calcium mobilization and prevented the formation of procoagulant platelets, highlighting the critical and dichotomous role of NCX during platelet activation induced by convulxin-plus-thrombin.
THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Immunology
Jurij Kiefer, Johannes Zeller, Balazs Bogner, Isabel A. Hoerbrand, Friederike Lang, Emil Deiss, Oscar Winninger, Mark Fricke, Sheena Kreuzaler, Eva Smudde, Markus Huber-Lang, Karlheinz Peter, Kevin J. Woollard, Steffen U. Eisenhardt
Summary: This paper presents a flow cytometry-based protocol for evaluating subset-specific activation and cytokine expression of circulating blood monocytes in humans and rats, offering an unbiased functional endpoint analysis for studying the differential regulation of monocytes in inflammatory and allogeneic immune responses.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Ayesha Singh, Amanda R. Coulter, Patrick J. Trainor, Narayana Sarma Singam, Bahjat N. Aladili, Alok R. Amraotkar, Ugochukwu S. Owolabi, Andrew P. DeFilippis
Summary: Delaying sample staining negatively affects the measurement of PMC via FCM, with a 30% decrease in PMC measurement within the first 24 hours. It is recommended to complete staining and FCM analysis within an hour of sample collection.
JOURNAL OF THROMBOSIS AND THROMBOLYSIS
(2021)
Article
Medicine, General & Internal
Raul Pineiro-Salvador, Eduardo Vazquez-Garza, Jose Antonio Cruz-Cardenas, Cuauhtemoc Licona-Cassani, Gerardo Garcia-Rivas, Jorge Moreno-Vasquez, Mario Rene Alcorta-Garcia, Victor Javier Lara-Diaz, Marion E. G. Brunck
Summary: This study provides the first characterization of major leukocyte subsets in colostrum of mothers suffering from obesity and the first report of colostrum leukocyte subpopulations in Latin America. The study reveals a decreased colostrum B lymphocyte fraction in obesity.
Article
Hematology
Maria N. Barrachina, Lidia Hermida-Nogueira, Luis A. Moran, Vanessa Casas, Sarah M. Hicks, Aurelio M. Sueiro, Ying Di, Robert K. Andrews, Steve P. Watson, Elizabeth E. Gardiner, Joaquin Abian, Montserrat Carrascal, Maria Pardo, Angel Garcia
Summary: In this study, a comparative phosphoproteomic analysis of platelets from obese patients revealed up-regulated levels of phosphorylation events and altered signaling pathways compared to lean controls. Flow cytometry assays and mass spectrometry validation confirmed platelet dysfunction in obese individuals, including higher surface levels of GPVI and CLEC2. Soluble GPVI levels were also found to be elevated in plasma from obese patients, suggesting increased GPVI signaling activation.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Article
Cell Biology
Linda G. Rikkert, Frank A. W. Coumans, Chi M. Hau, Leon W. M. M. Terstappen, Rienk Nieuwland
Summary: A study developed a fast and simple single-step centrifugation protocol for producing high-quality platelet-free plasma for EV research. Compared to the traditional two-step protocol, the single-step protocol is faster, removes platelets effectively, and has a slightly higher plasma yield.
Article
Hematology
Georges Jourdi, Sofia Ramstrom, Ruchika Sharma, Tamam Bakchoul, FC-PFT TP Study Grp
Summary: The aim of this study was to provide expert consensus guidance on the use of flow cytometry for the evaluation of platelet function in patients with low platelet counts. Through literature review and focus group discussions, consensus recommendations were made on sample collection, preanalytical variables, sample type, and results expression. These recommendations would allow standardization of protocols in future clinical studies.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Hematology
Julia Moosmann, Anja Krusemark, Sven Dittrich, Tatjana Ammer, Manfred Rauh, Joachim Woelfle, Markus Metzler, Jakob Zierk
Summary: This study calculated reference intervals for NLR, PLR, and LMR from birth to 18 years using a data-mining approach, and investigated their diagnostic benefit in different inflammatory diseases. The results showed that NLR was significantly increased in patients with appendicitis and asthma, while PLR and LMR were not sensitive markers for the examined inflammatory diseases.
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
(2022)
Article
Biology
Masashi Ugawa, Yoko Kawamura, Keisuke Toda, Kazuki Teranishi, Hikari Morita, Hiroaki Adachi, Ryo Tamoto, Hiroko Nomaru, Keiji Nakagawa, Keiki Sugimoto, Evgeniia Borisova, Yuri An, Yusuke Konishi, Seiichiro Tabata, Soji Morishita, Misa Imai, Tomoiku Takaku, Marito Araki, Norio Komatsu, Yohei Hayashi, Issei Sato, Ryoichi Horisaki, Hiroyuki Noji, Sadao Ota
Summary: This study introduces a high-throughput stain-free flow cytometry called in silico-labeled ghost cytometry, which characterizes and sorts cells using machine-predicted labels.
Article
Hematology
Alexey A. Martyanov, Anna E. Boldova, Maria G. Stepanyan, Olga An, Alexander S. Gur'ev, Darya Kassina, Alexey Y. Volkov, Alexandr Balatskiy, Andrei A. Butylin, Sergei S. Karamzin, Elena Filimonova, Sergei Tsarenko, Sergei A. Roumiantsev, Alexander G. Rumyantsev, Mikhail A. Panteleev, Fazoil Ataullakhanov, Anastasia N. Sveshnikova
Summary: Patients with COVID-19 exhibit platelet dysfunction, characterized by increased platelet reactivity, decreased membrane protein shedding, and reduced aggregation. Treatment with LMWH can improve platelet parameters, while in vitro experiments confirm the platelet refractoriness pattern.
THROMBOSIS RESEARCH
(2022)
Article
Cell Biology
Naomi C. Buntsma, Aleksandra Gasecka, Yvo B. W. E. M. Roos, Ton G. van Leeuwen, Edwin van der Pol, Rienk Nieuwland
Summary: In this study, we found that EDTA improves the stability of plasma EVs and is more effective at inhibiting the release of platelet EVs compared to citrate when platelets are activated.
Article
Hematology
Fatemeh Shahneh, Hans Christian Probst, Sabine C. Wiesmann, Noelia A-Gonzalez, Wolfram Ruf, Kerstin Steinbrink, Verena K. Raker, Christian Becker
Summary: This study investigated the role of inflammatory Ly6C(hi) monocytes in deep vein thrombosis, and found that thrombus formation, growth, and resolution can be therapeutically manipulated with a NR4A1 agonist.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2022)
Article
Immunology
David A. Simon A. Davis, Melissa Ritchie, Dillon Hammill, Jessica Garrett, Robert O. O. Slater, Naomi Otoo, Anna Orlov, Katharine Gosling, Jason Price, Desmond Yip, Kylie Jung, Farhan M. M. Syed, Ines I. I. Atmosukarto, Ben J. C. Quah
Summary: Blood immune cell signatures can serve as biomarkers for cancer progression, aiding in detection and characterization of different types of cancer.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Critical Care Medicine
Suelyn Van Den Helm, Hui Ping Yaw, Natasha Letunica, Rebecca Barton, Asami Weaver, Fiona Newall, Stephen B. Horton, Roberto Chiletti, Amy Johansen, Derek Best, Joanne McKittrick, Warwick Butt, Yves d'Udekem, Graeme MacLaren, Matthew D. Linden, Vera Ignjatovic, Paul Monagle
Summary: This study investigated the platelet pathophysiology in pediatric ECMO patients and found that the platelet response decreased over time, which may contribute to bleeding during ECMO. These findings are important for understanding the mechanisms of bleeding in pediatric ECMO and predicting or detecting bleeding and thrombosis.
CRITICAL CARE MEDICINE
(2022)
Article
Gastroenterology & Hepatology
Antti Turunen, Antti Kuuliala, Harri Mustonen, Pauli Puolakkainen, Leena Kylanpaa, Krista Kuuliala
Summary: The phosphorylation levels of leukocyte signaling molecules can potentially predict the severity of acute pancreatitis, including organ dysfunction and secondary infections in patients. Different phosphorylation levels are associated with different prognosis outcomes.
Article
Cardiac & Cardiovascular Systems
Ernst J. Schaefer, Hiroaki Ikezaki, Margaret R. Diffenderfer, Elise Lim, Ching -Ti Liu, Ron C. Hoogeveen, Weihua Guan, Michael Y. Tsai, Christie M. Ballantyne
Summary: The study aimed to determine whether direct and calculated sdLDL-C were significant independent ASCVD risk factors in sex and race subgroups. The results showed that both direct and calculated sdLDL-C were significantly associated with ASCVD, but the association was stronger for direct sdLDL-C and not significant for calculated values once direct values were included in the model. Direct sdLDL-C had a significant independent association with incident ASCVD in all subgroups except African Americans.
Article
Biochemistry & Molecular Biology
Michael R. R. Duggan, Lauren Butler, Zhongsheng Peng, Gulzar N. N. Daya, Abhay Moghekar, Yang An, Stephen R. R. Rapp, Kathleen M. M. Hayden, Aladdin H. H. Shadyab, Ginny Natale, Longjian Liu, Linda Snetselaar, Ruin Moaddel, Casey M. M. Rebholz, Kevin Sullivan, Christie M. M. Ballantyne, Susan M. M. Resnick, Luigi Ferrucci, Keenan A. A. Walker
Summary: Dysregulation of the immune system and inflammatory dietary patterns may increase the risk for cognitive decline. Proteomic assays of plasma samples identified a group of proteins linked to future cognitive impairment, as well as associations with dementia risk and biomarkers of Alzheimer's disease and neurodegeneration.
MOLECULAR PSYCHIATRY
(2023)
Article
Cardiac & Cardiovascular Systems
Olga A. Gimnich, Tatiana Belousova, Christina M. Short, Addison A. Taylor, Vijay Nambi, Joel D. Morrisett, Christie M. Ballantyne, Jean Bismuth, Dipan J. Shah, Gerd Brunner
Summary: Researchers developed a computational microvascular model to study peripheral artery disease (PAD) using contrast-enhanced magnetic resonance imaging signal intensities. They found significant associations between the model parameters and known markers of PAD patients, as well as significant differences between PAD patients and matched controls.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2023)
Letter
Cardiac & Cardiovascular Systems
Salim S. S. Virani, David J. J. Ramsey, Dax Westerman, Mark K. K. Kuebeler, Liang Chen, Julia M. M. Akeroyd, Glenn T. T. Gobbel, Christie M. M. Ballantyne, Laura A. A. Petersen, Alexander Turchin, Michael E. E. Matheny
Letter
Cardiac & Cardiovascular Systems
Yiyi Zhang, Jacqueline S. Dron, Brandon K. Bellows, Amit V. Khera, Junxiu Liu, Pallavi P. Balte, Elizabeth C. Oelsner, Sami Samir Amr, Matthew S. Lebo, Anna Nagy, Gina M. Peloso, Pradeep Natarajan, Jerome I. Rotter, Cristen Willer, Eric Boerwinkle, Christie M. Ballantyne, Pamela L. Lutsey, Myriam Fornage, Donald M. Lloyd-Jones, Lifang Hou, Bruce M. Psaty, Joshua C. Bis, James S. Floyd, Ramachandran S. Vasan, Nancy L. Heard-Costa, April P. Carson, Michael E. Hall, Stephen S. Rich, Xiuqing Guo, Dhruv S. Kazi, Sarah D. de Ferranti, Andrew E. Moran
Article
Cardiac & Cardiovascular Systems
Nicolas Girerd, Daniel Levy, Kevin Duarte, Joao Pedro Ferreira, Christie Ballantyne, Timothy Collier, Anne Pizard, Jens Bjoerkman, Javed Butler, Andrew Clark, John G. Cleland, Christian Delles, Javier Diez, Arantxa Gonzalez, Mark Hazebroek, Jennifer Ho, Anne-Cecile Huby, Shih-Jen Hwang, Roberto Latini, Beatrice Mariottoni, Alexandre Mebazaa, Pierpaolo Pellicori, Naveed Sattar, Peter Sever, Jan A. Staessen, Job Verdonschot, Stephane Heymans, Patrick Rossignol, Faiez Zannad
Summary: This study identified protein biomarkers associated with new-onset heart failure in three independent cohorts. The inclusion of these biomarkers in addition to clinical risk factors improved the prediction of heart failure risk. The B-type natriuretic peptide and N-terminal pro-B-type natriuretic peptide were found to be particularly relevant in predicting heart failure.
CIRCULATION-HEART FAILURE
(2023)
Article
Endocrinology & Metabolism
Suzanne V. Arnold, James A. de Lemos, Luke Zheng, Robert S. Rosenson, Christie M. Ballantyne, Shushama Alam, Deepak L. Bhatt, Christopher P. Cannon, Mikhail Kosiborod, GOULD Investigators
Summary: The study aimed to examine the improvement in the use of optimal medical therapy (OMT) for patients with atherosclerotic cardiovascular disease (ASCVD) and diabetes. The study found that the use of secondary prevention in US patients with ASCVD and diabetes was suboptimal, with minimal improvement over time.
DIABETES OBESITY & METABOLISM
(2023)
Article
Pharmacology & Pharmacy
Paul M. Ridker, Lei Lei, Kausik K. Ray, Christie M. Ballantyne, Gary Bradwin, Nader Rifai
Summary: Bempedoic acid (BA) reduces hsCRP and LDL-C levels in patients with atherosclerotic disease and/or familial hypercholesterolemia. It also shows potential anti-inflammatory effects and has similar lipid-lowering and inflammation-inhibiting properties to statin therapy.
JOURNAL OF CLINICAL LIPIDOLOGY
(2023)
Editorial Material
Cardiac & Cardiovascular Systems
Christie M. Ballantyne, Mini Grace Varughese, Layla A. Abushamat
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Christie M. Ballantyne, Puja Banka, Gustavo Mendez, Raymundo Garcia, Julio Rosenstock, Anthony Rodgers, Geraldine Mendizabal, Yale Mitchel, Alberico L. Catapano
Summary: MK-0616, an oral PCSK9 inhibitor, effectively reduces LDL-C levels and is well tolerated in the treatment of hypercholesterolemia according to a Phase 2b clinical trial.
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Lena Mathews, Xiao Hu, Ning Ding, Junichi Ishigami, Mahmoud Al Rifai, Ron C. C. Hoogeveen, Josef Coresh, Christie M. M. Ballantyne, Elizabeth Selvin, Kunihiro Matsushita
Summary: A study on 9205 participants from the ARIC study found that higher levels of GDF15 protein were associated with bleeding events and improved the prediction of bleeding risk in individuals without cardiovascular disease.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2023)
Article
Cardiac & Cardiovascular Systems
Marina Cuchel, Paul C. Lee, Lisa C. Hudgins, P. Barton Duell, Zahid Ahmad, Seth J. Baum, MacRae F. Linton, Sarah D. de Ferranti, Christie M. Ballantyne, John A. Larry, Linda C. Hemphill, Iris Kindt, Samuel S. Gidding, Seth S. Martin, Patrick M. Moriarty, Paul P. Thompson, James A. Underberg, John R. Guyton, Rolf L. Andersen, David J. Whellan, Irwin Benuck, John P. Kane, Kelly Myers, William Howard, David Staszak, Allison Jamison, Mary C. Card, Mafalda Bourbon, Joana R. Chora, Daniel J. Rader, Joshua W. Knowles, Katherine Wilemon, Mary P. McGowan
Summary: This study investigated the diagnosis and treatment of HoFH in the United States and found a low diagnosis rate and low treatment rate, highlighting the need for enhanced screening and treatment to reduce the burden of the disease.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2023)
Article
Cardiac & Cardiovascular Systems
Brian Olshansky, Deepak L. L. Bhatt, Michael Miller, Ph. Gabriel Steg, Eliot A. A. Brinton, Terry A. A. Jacobson, Steven B. B. Ketchum, Ralph T. T. Doyle Jr, Rebecca A. A. Juliano, Lixia Jiao, Peter R. R. Kowey, James A. A. Reiffel, Jean-Claude Tardif, Christie M. M. Ballantyne, Mina K. K. Chung
Summary: In the REDUCE-IT study, icosapent ethyl (IPE) reduced cardiovascular events but was associated with increased atrial fibrillation/atrial flutter (AF) hospitalization. The study analyzed the relationships between IPE and outcomes in patients with or without prior AF and with or without in-study AF hospitalization. Overall, in-study AF hospitalization rates were higher in patients with prior AF, especially in those randomized to IPE. Serious bleeding rates also trended higher in these patients. However, patients with prior AF or in-study AF hospitalization showed consistent risk reductions in cardiovascular events with IPE.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2023)
Article
Cardiac & Cardiovascular Systems
Xiaoming Jia, Mahmoud Al Rifai, Ron Hoogeveen, Justin B. Echouffo-Tcheugui, Amil M. M. Shah, Chiadi E. Ndumele, Salim S. Virani, Biykem Bozkurt, Elizabeth Selvin, Christie M. Ballantyne, Vijay Nambi
Summary: This study assessed the association between change in NT-proBNP and the risk for incident heart failure and death. The results showed that change in NT-proBNP was positively correlated with the risk of heart failure and death. Additionally, changes in blood pressure, cholesterol, triglyceride levels, body mass index, and estimated glomerular filtration rate were associated with change in NT-proBNP.
Article
Cardiac & Cardiovascular Systems
Austin Deets, Parag H. Joshi, Alvin Chandra, Kavisha Singh, Amit Khera, Salim S. Virani, Christie M. Ballantyne, James D. Otvos, Robin P. F. Dullaart, Eke G. Gruppen, Margery A. Connelly, Colby Ayers, Ann Marie Navar, Ambarish Pandey, John T. Wilkins, Anand Rohatgi
Summary: This study aimed to assess the association between levels of specific size-based HDL subspecies and atherosclerotic cardiovascular disease, and investigate their role in risk prediction.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2023)