Article
Engineering, Biomedical
Mee-Hae Kim, Shao Ying Tan, Kenichi Yamahara, Masahiro Kino-Oka
Summary: Assessing the ability of cultured cells to degrade extracellular matrix (ECM) is crucial for evaluating the effectiveness of mesenchymal stem cell (MSC)-based therapies. However, current methods to assess this ability in vitro are lacking. In this study, a simple culture platform using high-density collagen surfaces was developed to study the ECM remodeling potential of cultured MSCs. This platform was used to evaluate the ECM remodeling potential of long-term ex vivo-expanded MSCs in vitro.
ACTA BIOMATERIALIA
(2023)
Article
Biochemistry & Molecular Biology
Janina Burk, Anna Sassmann, Cornelia Kasper, Ariane Nimptsch, Susanna Schubert
Summary: Matrix remodeling by MSC in ECM disease could be influenced by the ECM environment. MSC adapt to the ECM conditions, including pathological adaptations, which may affect therapeutic efficacy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Carla U. Doll, Sabine Niebert, Janina Burk
Summary: Tendon lesions are common in both humans and horses, with the healing process often leading to fibrosis and chronic disease. Local mesenchymal stromal cell injection is a accepted therapeutic strategy for acute lesions in horses, but data on its efficacy in chronic tendon disease are limited. However, MSCs have been shown to have a positive effect on fibrotic tissue. This study aimed to investigate the interaction between MSCs and healthy or chronically diseased tendon matrix in horses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Umberto Bracale, Roberto Peltrini, Biancamaria Iacone, Mirko Martirani, Daniele Sannino, Antonio Gargiulo, Francesco Corcione, Raffaele Serra, Umberto Marcello Bracale
Summary: This study aimed to investigate the relationship between extracellular matrix disorders and abdominal wall hernias (AWHs) and the levels of matrix metalloproteinases (MMPs). The results showed that AWHs may be a disease of the extracellular matrix, supported by increased levels of MMPs in patients with AWHs. The study also highlighted the impact of tissue inhibitors of metalloproteinases (TIMPs) on AWHs. More prospective studies are needed to confirm the relationship between these biomarkers and the risk of AWHs.
Review
Biochemistry & Molecular Biology
Giuseppe Filiberto Serraino, Federica Jiritano, Davide Costa, Nicola Ielapi, Desiree Napolitano, Pasquale Mastroroberto, Umberto Marcello Bracale, Michele Andreucci, Raffaele Serra
Summary: Hypertrophic cardiomyopathy (HCM) is a genetic condition characterized by altered collagen turnover of the extracellular matrix. Matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) are abnormally released in patients with HCM. This systematic review found that MMP levels, particularly MMP2, were higher in HCM patients compared to healthy subjects. MMPs can be used as biomarkers for HCM and for monitoring treatment outcomes.
Article
Cardiac & Cardiovascular Systems
Alwin de Jong, Vincent Q. Sier, Hendrika A. B. Peters, Natalia K. M. Schilder, J. Wouter Jukema, Marie Jose T. H. Goumans, Paul H. A. Quax, Margreet R. de Vries
Summary: This study investigated the role of ALK1 signaling in vein graft disease, revealing that reduced ALK1 signaling promotes outward remodeling, increases macrophage influx, and promotes an unstable plaque phenotype in murine vein grafts.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Review
Oncology
Zoi Piperigkou, Konstantina Kyriakopoulou, Christos Koutsakis, Stylianos Mastronikolis, Nikos K. Karamanos
Summary: Proteolytic enzymes and non-proteolytic enzymatic partners play crucial roles in cancer cell propagation and metastatic potential. This article revisits the functions of major matrix remodeling molecules and discusses their epigenetic regulation mechanisms, pharmacological targeting, and ongoing clinical trials. The importance of targeting matrix remodeling partners for cancer management is explored in relation to tissue homeostasis and ECM turnover.
Article
Multidisciplinary Sciences
Yaraslau Padrez, Lena Golubewa, Tatsiana Kulahava, Tatyana Vladimirskaja, Galina Semenkova, Igor Adzerikho, Olga Yatsevich, Nadezda Amaegberi, Renata Karpicz, Yuri Svirko, Polina Kuzhir, Danielis Rutkauskas
Summary: We demonstrated that wide-field second harmonic generation (SHG) microscopy combined with quantitative analysis is a powerful tool for visualizing the fibrosis pathogenesis in pulmonary arterial hypertension (PAH) in a fast and label-free manner. Statistical analysis of SHG images revealed changes in collagen content and morphology in lung tissue during PAH progression.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Matteo Giovarelli, Francesca Arnaboldi, Silvia Zecchini, Laura Brigida Cornaghi, Ambra Nava, Michele Sommariva, Emilio Giuseppe Ignazio Clementi, Nicoletta Gagliano
Summary: This study provides a comprehensive histological and molecular characterization of muscle fibrosis in Duchenne muscular dystrophy (DMD), showing that fibrosis mainly affects the diaphragm and quadriceps with higher collagen cross-linking and inhibition of MMPs. These findings may lead to new targeted therapeutic interventions for DMD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ryo Inoue, Taro Yasuma, Valeria Fridman D'Alessandro, Masaaki Toda, Toshiyuki Ito, Atsushi Tomaru, Corina N. N. D'Alessandro-Gabazza, Tatsuki Tsuruga, Tomohito Okano, Atsuro Takeshita, Kota Nishihama, Hajime Fujimoto, Tetsu Kobayashi, Esteban C. C. Gabazza
Summary: This study evaluated the role of matrix metalloproteinase-2 (MMP-2) in the development of pulmonary fibrosis. Transgenic mice overexpressing human MMP-2 showed decreased lung inflammation and fibrosis compared to wild-type mice, following bleomycin-induced lung injury. MMP-2 appears to protect against pulmonary fibrosis by inhibiting apoptosis of lung epithelial cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Elke Pach, Maike Kuemper, Julia E. Fromme, Jan Zamek, Fabian Metzen, Manuel Koch, Cornelia Mauch, Paola Zigrino
Summary: The deletion of MMP14 in dermal fibroblasts leads to the deposition of an extracellular matrix that inhibits melanoma growth, with collagen XIV identified as a key regulator in melanoma progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, General & Internal
Viktor Domislovic, Joachim Hog Mortensen, Majken Lindholm, Morten Asser Kaarsdal, Marko Brinar, Ana Barisic, Tina Manon-Jensen, Zeljko Krznaric
Summary: This study investigated serum biomarkers of ECM remodeling in patients with inflammatory bowel disease (IBD), and found that a combination of C4M, C3M, and PRO-C3 biomarkers may potentially serve as a biomarker to differentiate moderate to severe endoscopic disease.
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Chemistry, Medicinal
Panagiotis Theofilis, Marios Sagris, Evangelos Oikonomou, Alexios S. Antonopoulos, George Lazaros, Anastasios Theofilis, Charalambos Papastamos, Spyridon Papaioannou, Gerasimos Siasos, Kostas Tsioufis, Dimitris Tousoulis
Summary: Atherosclerosis and coronary artery disease are significant causes of morbidity and mortality worldwide, requiring early detection and treatment. Extracellular matrix remodeling plays a crucial role in this process, and indicators of matrix degradation have been studied in relation to the existence, severity, and prognosis of coronary artery disease.
CURRENT TOPICS IN MEDICINAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Jagoda Kruszewska, Agnieszka Cudnoch-Jedrzejewska, Katarzyna Czarzasta
Summary: Obesity is a growing problem, affecting two-thirds of adults and increasing the risk of cardiovascular diseases. Chronic obesity can also lead to heart failure with preserved ejection fraction. Excessive collagen deposition in the extracellular matrix during obesity can stiffen the myocardium and impair heart function. This review discusses the rationale and potential factors behind this process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Tatjana Maravic, Eugenia Baena, Claudia Mazzitelli, Uros Josic, Edoardo Mancuso, Vittorio Checchi, Luigi Generali, Laura Ceballos, Lorenzo Breschi, Annalisa Mazzoni
Summary: This study evaluated the effect of different concentrations of chitosan polymer on dentinal enzymatic activity. Results showed that higher concentrations of chitosan were correlated with increased enzymatic activity, especially in the restored dentin groups. Chitosan at 0.1% concentration showed faint expression of enzymatic activity compared to higher concentrations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Meeting Abstract
Peripheral Vascular Disease
Xiaohong R. Xu, Yiming Wang, Reheman Adili, Lining Ju, Christopher M. Spring, Joseph W. Jin, Hong Yang, Pingguo Chen, Yan Yang, Xi Lei, Yunfeng Chen, Reid C. Gallant, Miao Xu, Miguel A. Neves, Hailong Zhang, Jina Song, Dan Zhang, Naadiya Carrim, Guangheng Zhu, Yi-Min She, Terry Cyr, Wenbin Fu, Guoqing Liu, Philip W. Connelly, Margaret L. Rand, Khosrow Adeli, John Freedman, Jeffrey E. Lee, Patrick Tso, Patrizia Marchese, W. S. Davidson, Shaun P. Jackson, Cheng Zhu, Zaverio M. Ruggeri, Heyu Ni
ATHEROSCLEROSIS SUPPLEMENTS
(2018)
Article
Anesthesiology
Gregory M. T. Hare, Kaho Han, Yevheniy Leshchyshyn, Nikhil Mistry, Tiffanie Kei, Si Yuan Dai, Albert K. Y. Tsui, Razak A. Pirani, Jaideep Honavar, Rakesh P. Patel, Sanjay Yagnik, Shelley L. Welker, Tessa Tam, Alexander Romaschin, Philip W. Connelly, W. Scott Beattie, C. David Mazer
CANADIAN JOURNAL OF ANESTHESIA-JOURNAL CANADIEN D ANESTHESIE
(2018)
Article
Nutrition & Dietetics
Xiaoran Liu, Josephine Garban, Peter J. Jones, Jack Vanden Heuvel, Benoit Lamarche, David J. Jenkins, Philip W. Connelly, Patrick Couture, Shuaihua Pu, Jennifer A. Fleming, Sheila G. West, Penny M. Kris-Etherton
JOURNAL OF NUTRITION
(2018)
Article
Endocrinology & Metabolism
Ravi Retnakaran, Chang Ye, Caroline K. Kramer, Anthony J. Hanley, Philip W. Connelly, Mathew Sermer, Bernard Zinman
Article
Nutrition & Dietetics
Janie Allaire, Denis Talbot, Patrick Couture, Andre Tchernof, Peter J. H. Jones, Penny Kris-Etherton, Sheila G. West, Philip W. Connelly, David J. A. Jenkins, Benoit Lamarche
EUROPEAN JOURNAL OF CLINICAL NUTRITION
(2019)
Article
Multidisciplinary Sciences
Xiaohong Ruby Xu, Yiming Wang, Reheman Adili, Lining Ju, Christopher M. Spring, Joseph Wuxun Jin, Hong Yang, Miguel A. D. Neves, Pingguo Chen, Yan Yang, Xi Lei, Yunfeng Chen, Reid C. Gallant, Miao Xu, Hailong Zhang, Jina Song, Peifeng Ke, Dan Zhang, Naadiya Carrim, Si-Yang Yu, Guangheng Zhu, Yi-Min She, Terry Cyr, Wenbin Fu, Guoqing Liu, Philip W. Connelly, Margaret L. Rand, Khosrow Adeli, John Freedman, Jeffrey E. Lee, Patrick Tso, Patrizia Marchese, W. Sean Davidson, Shaun P. Jackson, Cheng Zhu, Zaverio M. Ruggeri, Heyu Ni
NATURE COMMUNICATIONS
(2018)
Article
Cardiac & Cardiovascular Systems
Sadia Mehmood, Chang Ye, Philip W. Connelly, Anthony J. Hanley, Bernard Zinman, Ravi Retnakaran
CARDIOVASCULAR DIABETOLOGY
(2018)
Article
Respiratory System
Paul D. W. Eckford, Jacqueline McCormack, Lise Munsie, Gengming He, Sanja Stanojevic, Sergio L. Pereira, Karen Ho, Julie Avolio, Claire Bartlett, Jin Ye Yang, Amy P. Wong, Leigh Wellhauser, Ling Jun Huan, Jia Xin Jiang, Hong Ouyang, Kai Du, Michelle Klingel, Lianna Kyriakopoulou, Tanja Gonska, Theo J. Moraes, Lisa J. Strug, Janet Rossant, Felix Ratjen, Christine E. Bear
JOURNAL OF CYSTIC FIBROSIS
(2019)
Article
Multidisciplinary Sciences
Amy P. Wong, Sharareh Shojaie, Qin Liang, Sunny Xia, Michelle Di Paola, Saumel Ahmadi, Claudia Bilodeau, Jodi Garner, Martin Post, Pascal Duchesneau, Thomas K. Waddell, Christine E. Bear, Andras Nagy, Janet Rossant
SCIENTIFIC REPORTS
(2019)
Review
Medicine, Research & Experimental
Jin-A Lee, Alex Cho, Elena N. Huang, Yiming Xu, Henry Quach, Jim Hu, Amy P. Wong
Summary: The discovery of the Cystic fibrosis (CF) gene in 1989 has led to significant progress in treating the disease, with the average survival age of patients with CF reaching 58 years in Canada. Recent advancements in gene therapy approaches and new research models are rekindling the search for a permanent genetic cure for all CF.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Cell & Tissue Engineering
Jia Xin Jiang, Leigh Wellhauser, Onofrio Laselva, Irina Utkina, Zoltan Bozoky, Tarini Gunawardena, Zoe Ngan, Sunny Xia, Michelle Di Paola, Paul D. W. Eckford, Felix Ratjen, Theo J. Moraes, John Parkinson, Amy P. Wong, Christine E. Bear
Summary: This study introduced a new testing platform that uses patient-specific iPSCs differentiated to lung progenitor cells for high-throughput assay of CFTR channel activity. The proof-of-concept studies supported the potential use of this platform in developing therapies for rare CFTR mutations.
Review
Cell Biology
Elena N. Huang, Henry Quach, Jin-A Lee, Joshua Dierolf, Theo J. Moraes, Amy P. Wong
Summary: Cystic fibrosis is a disease caused by mutations in the CFTR protein, leading to abnormal fluid and ion transport, reduced lung function, and increased mortality rate. Although progress has been made in CF research, clinical outcomes may not be mutation-specific. Research suggests that CFTR protein plays an important role in early lung development and mutant CFTR proteins may impact CF airway disease.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Meeting Abstract
Cardiac & Cardiovascular Systems
Kate J. Bowen, Penny M. Kris-Etherton, Sheila G. West, Jennifer A. Fleming, Philip W. Connelly, Benoit Lamarche, Patrick Couture, David J. Jenkins, Carla Taylor, Peter Zahradka, Shatha S. Hammad, Jyoti Sihag, Xiang Chen, Valerie Guay, Julie Maltais-Giguere, Danielle Perera, Angela Wilson, Sandra Castillo, Peter J. Jones
Article
Endocrinology & Metabolism
Leanne R. De Souza, Chang Ye, Anthony J. Hanley, Philip W. Connelly, Mathew Sermer, Bernard Zinman, Ravi Retnakaran
CLINICAL ENDOCRINOLOGY
(2018)
Article
Endocrinology & Metabolism
Sadia Mehmood, Myles Margolis, Chang Ye, Louise Maple-Brown, Anthony J. Hanley, Philip W. Connelly, Mathew Sermer, Bernard Zinman, Ravi Retnakaran
BMJ OPEN DIABETES RESEARCH & CARE
(2018)