4.6 Article

Effect of donor animal species and their feeding on the composition of the microbial community establishing in a rumen simulation

期刊

JOURNAL OF APPLIED MICROBIOLOGY
卷 119, 期 1, 页码 33-46

出版社

OXFORD UNIV PRESS
DOI: 10.1111/jam.12829

关键词

donor animal species; fluorescence insitu hybridization; grass silage; maize silage; rumen simulation; ruminal microbial community

向作者/读者索取更多资源

AimsTo investigate the effect of donor animal species and their feeding on the composition of the active fraction of rumen microbiota established during invitro experiments with different forages. Methods and ResultsRumen simulation experiments were conducted with maize silage (MS) and grass silage (GS) as substrates. Four experimental runs were performed with ruminal contents of sheep and cows fed either with hay and concentrate or with MS or GS, respectively. Liquid- and solid-associated microbes (LAM and SAM) were fixed for fluorescence insitu hybridization after 14days of incubation. The LAM were characterized by higher proportions of Lachnospiraceae, Bacteroidetes, Deltaproteobacteria and Methanobacteriaceae at hay-concentrate-based feeding of the donor animals whereas counts of Gammaproteobacteria were lower. Similar results were found for SAM. Hay-concentrate-based feeding caused a higher ratio of Bacteria:Archaea in SAM. Incubations with GS seemed to enhance Bacteroidetes, Deltaproteobacteria and Archaea whereas numbers of Gammaproteobacteria were enhanced in MS experiments. The effect of the donor animal species was limited to the number of Archaea establishing invitro being higher for sheep than for cows. ConclusionsThe feeding of donor animals, more than the animal species itself, affects the composition of the ruminal microbial community that establishes invitro. Significance and Impact of the StudyData suggest the need of a standardized approach for studying the rumen microbiota in a rumen simulation. Moreover, this study provides fundamental data on the composition of the ruminal microbial community when different diets are fed to donor animals.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

Article Biochemistry & Molecular Biology

Mineral and Phytic Acid Content as Well as Phytase Activity in Flours and Breads Made from Different Wheat Species

C. Friedrich. H. Longin, Muhammad Afzal, Jens Pfannstiel, Ute Bertsche, Tanja Melzer, Andrea Ruf, Christoph Heger, Tobias Pfaff, Margit Schollenberger, Markus Rodehutscord

Summary: Wheat is an important source of minerals for a healthy diet, but the phytate complex in the grain makes some minerals unavailable to humans. Our trials compared different wheat species and bread-making recipes to find ways to reduce phytic acid content and increase mineral bioavailability. We found significant variations in mineral content and phytase activity among different wheat species and varieties. Bread-making recipes, such as long yeast proofing or the use of whole grain rye flour, had a greater impact on reducing phytic acid content in bread than the choice of wheat species. Collaborations between research organizations and stakeholders are necessary for optimizing food production for a growing world population.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)

Article Agriculture, Dairy & Animal Science

Inferring causal structures of gut microbiota diversity and feed efficiency traits in poultry using Bayesian learning and genomic structural equation models

Valentin Haas, Markus Rodehutscord, Amelia Camarinha-Silva, Joern Bennewitz

Summary: Feed and phosphorus efficiency are important in poultry breeding, and they are influenced by the gut microbiota composition. In this study, data from 749 individuals of a Japanese quail F2 cross was used to analyze the relationship between gut microbiota composition and efficiency traits. The results showed that the diversity index of the gut microbiota had direct and indirect effects on phosphorus utilization and retention.

JOURNAL OF ANIMAL SCIENCE (2023)

Article Agriculture, Dairy & Animal Science

Minimum phosphorus requirements for laying hen feed formulations

Markus Rodehutscord, Vera Sommerfeld, C. Roselina Angel, Douglas R. Korver

Summary: The objective of this contribution is to summarize the optimal concentration of nonphytate phosphorus (NPP) in feed for laying hens from scientific literature. The data suggests that the NPP concentration in feed should not exceed 2.2 g NPP/kg without the use of phytase, as it shows no positive effect on performance and eggshell. The addition of phytase to the feed can reduce this value, but precise values of reduction cannot be calculated due to insufficient data.

POULTRY SCIENCE (2023)

Article Agriculture, Dairy & Animal Science

Effect of increasing dietary fermentable fiber on diet nutrient digestibility and estimation of endogenous phosphorus losses in growing pigs

Charlotte M. E. Heyer, Li Fang Wang, Eduardo Beltranena, Markus Rodehutscord, Ruurd T. Zijlstra

Summary: This study investigated the effects of feeding increasing acacia gum on the apparent ileal and total tract digestibility of nutrients in growing pigs. The results showed that increasing acacia gum reduced the digestibility of dry matter and gross energy, but did not affect the digestibility of crude protein and amino acids. However, it decreased the total tract digestibility of phosphorus. Feeding fermentable, low-viscous acacia gum can affect the digestion of pigs, and the specific endogenous losses of phosphorus associated with it should be considered in evaluating phosphorus digestibility.

JOURNAL OF ANIMAL SCIENCE (2023)

Article Agriculture, Dairy & Animal Science

Mucosal phosphatase activity, phytate degradation, and mineral digestibility in 6-week-old turkeys and broilers at different dietary levels of phosphorus and phytase and comparison with 3-week-old animals

Moritz Novotny, Vera Sommerfeld, Jochen Krieg, Imke Kuehn, Korinna Huber, Markus Rodehutscord

Summary: This study compared female turkeys and broilers at 6 weeks of age to assess the impact of species, dietary P, Ca, and phytase levels on InsP6 degradation, phosphatase activity, P and Ca digestibility, and myo-inositol concentrations. Different diets were fed to the animals for 7 days, and samples were collected from various parts of the digestive tract. The results showed differences in phytate degradation between turkeys and broilers, which could be attributed to age-related changes in the digestive tract.

POULTRY SCIENCE (2023)

Article Agriculture, Dairy & Animal Science

Amino acid digestibility and metabolizable energy of soybean meal of different origins in cecectomized laying hens1

W. Siegert, S. Kuenz, W. Windisch, M. Rodehutscord

Summary: This study investigated the variation in amino acid (AA) digestibility and MEn of 18 samples of solvent-extracted soybean meal (SBM) in cecectomized laying hens. The results showed that the digestibility of SBM differed among AA, with ranges in digestibility of 6 to 12%-units for most AA. The precision of feed formulation benefits from considering the variations in AA digestibility and MEn.

POULTRY SCIENCE (2023)

Article Agriculture, Dairy & Animal Science

Research Note: Influence of monocalcium phosphate and phytase in the diet on phytate degradation in cecectomized laying hens

W. Siegert, V. Sommerfeld, M. Schollenberger

Summary: This study investigated the effects of phytase and monocalcium phosphate supplementation on the dephosphorylation of phytic acid in laying hens. The results showed that phytase significantly increased the degradation of phytic acid, while monocalcium phosphate had no effect.

POULTRY SCIENCE (2023)

Article Agriculture, Dairy & Animal Science

Comparison of mucosal phosphatase activity, phytate degradation, and nutrient digestibility in 3-week-old turkeys and broilers at different dietary levels of phosphorus and phytase

Moritz Novotny, Vera Sommerfeld, Jochen Krieg, Imke Kuehn, Korinna Huber, Markus Rodehutscord

Summary: A comparison between 3-week-old female turkeys and broilers was conducted to investigate the impact of species, dietary P, Ca, and phytase levels on gut mucosal phosphatase activity, InsP6 degradation, nutrient digestibility, and myo-inositol concentrations. The results showed that both species have similar intrinsic capacity for phytate degradation when fed low P and Ca diets, but differ in InsP degradation and nutrient digestibility in certain segments of the digestive tract.

POULTRY SCIENCE (2023)

Article Virology

Dynamic Development of Viral and Bacterial Diversity during Grass Silage Preservation

Johan S. Saenz, Bibiana Rios-Galicia, Bianca Rehkugler, Jana Seifert

Summary: Ensilaging is a common process for preserving feed, using lactic acid bacteria to stabilize feed quality. This study investigated the composition of bacterial and viral communities during grass silage preservation, revealing a correlation between changes in pH, bacterial composition, and viral diversity. The results suggest that viral communities play a role in the establishment of the bacterial community during silage preservation.

VIRUSES-BASEL (2023)

Article Nutrition & Dietetics

The influence of substituting dietary peptide-bound with free amino acids on nitrogen metabolism and acid-base balance of broiler chickens depends on asparagine and glutamine supply

Ahmad Ibrahim, Akos Kenez, Markus Rodehutscord, Wolfgang Siegert

Summary: Reducing crude protein concentration and including free amino acids in animal feed can help mitigate the environmental impact of animal farming. This study examined the upper limits of free amino acid inclusion in the diet without negative effects on amino acid supply. Results showed that substituting 40-50% of digestible amino acids with free amino acids improved feed utilization.

BRITISH JOURNAL OF NUTRITION (2023)

Article Agriculture, Dairy & Animal Science

Effects of Acacia mearnsii added to silages differing in nutrient composition and condensed tannins on ruminal and manure-derived methane emissions of dairy cows

G. Lazzari, A. Munger, L. Eggerschwiler, D. Borda-Molina, J. Seifert, A. Camarinha-Silva, S. Schrade, M. Zahner, K. Zeyer, M. Kreuzer, F. Dohme-Meier

Summary: This study investigated the effects of acacia and sainfoin as condensed tannin-rich sources on ruminal and manure methane emissions. The results showed that neither acacia nor sainfoin reduced the methane yield or emission intensity. Acacia supplementation reduced milk production and dry matter intake, while sainfoin reduced milk protein content and yield. Therefore, a careful evaluation is necessary before implementing tanniferous feeds in farm practice, as it may result in productivity losses.

JOURNAL OF DAIRY SCIENCE (2023)

Review Agriculture, Multidisciplinary

Interactions of zinc with phytate and phytase in the digestive tract of poultry and pigs: a review

Hanna Philippi, Vera Sommerfeld, Wilhelm Windisch, Oluyinka Abiona Olukosi, Alessandra Monteiro, Markus Rodehutscord

Summary: Phytase supplementation is becoming important in animal nutrition due to its impact on phosphorus digestibility and the growing relevance of phosphorus for sustainable production. This review focuses on the interactions of zinc with phytate and phytase in the digestive tract of poultry and pigs, emphasizing the effects of zinc supplementation on phytase efficacy and phosphorus digestibility. In vitro studies show the inhibitory effect of zinc on phytase efficacy, but there is limited and inconsistent data from in vivo studies, possibly due to various factors and differences in methodology.

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2023)

Article Ecology

Microbiota and Nutrient Portraits of European Roe Deer (Capreolus capreolus) Rumen Contents in Characteristic Southern German Habitats

Sarah-Alica Dahl, Jana Seifert, Amelia Camarinha-Silva, Yu-Chieh Cheng, Angelica Hernandez-Arriaga, Martina Hudler, Wilhelm Windisch, Andreas Koenig

Summary: This study aims to investigate the rumen content of Bavarian roe deer and explore the composition of nutrients and microbiota. The results revealed significant differences in nutrient concentrations and microbial composition based on habitat, season, and age class. Additionally, a core bacterial community that plays a crucial role in the roe deer's rumen was identified.

MICROBIAL ECOLOGY (2023)

Article Biochemistry & Molecular Biology

Microbiome-derived cobalamin and succinyl-CoA as biomarkers for improved screening of anal cancer

Sergio Serrano-Villar, Camilla Tincati, Sajan C. Raju, Johan S. Saenz, Elena Moreno, Rafael Bargiela, Alfonso Cabello-Ubeda, Elena Sendagorta, Alina Kurz, Jose Perez A. Molina, Amparo de Benito, Johannes R. Hov, Laura Fernandez-Lopez, Alfonso Muriel, Rosa del Campo, Santiago Moreno, Marius Troseid, Jana Seifert, Manuel Ferrer

Summary: Human papillomavirus can lead to HSILs in the anogenital area, and the microbiome may play a role. HIV-MSM individuals have a high risk of anal cancer, but current screening methods for HSILs lack specificity. This study found that elevated levels of microbiome-encoded proteins were associated with HSILs, and measuring these biomarkers improved the current strategy of anal cancer screening.

NATURE MEDICINE (2023)

Article Agriculture, Dairy & Animal Science

Effect of dietary calcium concentration and exogenous phytase on inositol phosphate degradation, mineral digestibility, and gut microbiota in growing pigs

Nicolas Klein, Naomi Sarpong, Tanja Melzer, Dieter Feuerstein, Charlotte M. E. Heyer, Amelia Camarinha-Silva, Markus Rodehutscord

Summary: This study assessed the effects of dietary Ca concentration and exogenous phytase on InsP degradation, nutrient digestion and retention, blood metabolites, and microbiota composition in growing pigs. The results showed that additional Ca reduced the effect of exogenous phytase on prececal InsP6 degradation, but did not affect the abundance of bacterial genera in feces.

JOURNAL OF ANIMAL SCIENCE (2023)

暂无数据