Article
Chemistry, Analytical
W. Cheuquepan, S. Hernandez, M. Perez-Estebanez, L. Romay, A. Heras, A. Colina
Summary: Electrochemistry can be used to generate SERS substrates, utilizing the plasmonic properties of nanos-tructures and the chemical mechanism, with time-resolved Raman spectroelectrochemistry providing valuable information. Comparing Au and Ag metals for folic acid detection in drugs, it was found that Ag SERS substrates outperformed Au SERS substrates in complex samples.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Astronomy & Astrophysics
Darren A. Whitaker, Tasnim Munshi, Ian J. Scowen, Howell G. M. Edwards
Summary: The likelihood of finding intact cellular structures on the surface or in the near subsurface of Mars is slim due to intense bombardment of the surface by ionizing radiation. Therefore, it is more logical to search for evidence of microbial life by looking for molecules produced by the breakdown of cellular matter, known as biomarkers. Raman spectroscopy, a method used for detecting organic biomarkers among inorganic geomaterials, has low signal intensity on the martian surface due to the low concentration of organics.
Review
Chemistry, Multidisciplinary
Jan Krajczewski, Robert Ambroziak, Andrzej Kudelski
Summary: Surface-Enhanced Raman Scattering (SERS) refers to the phenomenon where the efficiency of generating Raman spectra by molecules adsorbed on some substrates is many orders of magnitude larger than those that are not adsorbed. Significant advances have been made in utilizing nanostructured non-metallic materials as SERS substrates, showing great potential for future applications.
Review
Biochemistry & Molecular Biology
Yulia Khristoforova, Lyudmila Bratchenko, Ivan Bratchenko
Summary: This review discusses the applications of Raman spectroscopy in medical diagnostics, including conventional Raman spectroscopy and surface-enhanced Raman spectroscopy. These techniques have been used to rapidly measure specific biomarkers of diseases such as cardiovascular disease, cancer, neurodegenerative disease, and COVID-19. However, there are still challenges and limitations that need to be addressed for their translation into clinical settings.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Jean-Francois Bryche, Marlo Vega, Agnes Tempez, Thibault Brule, Thomas Carlier, Julien Moreau, Marc Chaigneau, Paul G. Charette, Michael Canva
Summary: This work demonstrates the enhancement in plasmonic sensing efficacy by spatially-localized functionalization on nanostructured surfaces, where probe molecules are concentrated in areas of high field concentration. SERS measurements on nanostructured surfaces with homogeneous and spatially-localized functionalization with thiophenol show that the Raman signal mainly originates from high field concentration areas. TERS measurements with 10 nm spatial resolution confirm the predicted field distribution profiles.
Article
Spectroscopy
Arti Saroj, Uttam Sharma, Santanu Das, Venkatnarayan Ramanathan
Summary: This study explores the potential of non-noble metal substrates for Surface-enhanced Raman spectroscopy (SERS) and prepares a nanoplate beta-Bi2O3/Bi2O2CO3 heterostructure as a novel SERS substrate. The results demonstrate that the SERS signal on this substrate is significantly enhanced by adsorbed probe molecules.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Hajir Hilal, Mohammadnavid Haddadnezhad, Myeong Jin Oh, Insub Jung, Sungho Park
Summary: Elongated pseudohollow nanoframes composed of four rectangular plates enclosing the sides and two open-frame ends with four ridges pointing at the tips for near-field focusing are reported. The nanoframes are hollow inside, allowing the gaseous analyte to penetrate through the entire architecture and enabling efficient detection of gaseous analytes when combined with Raman spectroscopy. The significant enhancement in SERS is attributed to the presence of a flat solid terrace for a large surface area, sharp edges and vertices for strong electromagnetic near-field collection, and open frames for effective analyte transport and capture.
Article
Chemistry, Multidisciplinary
Myoung Gyu Kim, Miyeon Jue, Kwan Hee Lee, Eun Yeong Lee, Yeonjeong Roh, Minju Lee, Hyo Joo Lee, Sanghwa Lee, Huifang Liu, Bonhan Koo, Yoon Ok Jang, Eui Yeon Kim, Qiao Zhen, Sung-Han Kim, Jun Ki Kim, Yong Shin
Summary: The study introduces a deep learning assisted ZnO-Au-SERS-based direct amplification (ZADA) system for rapid, sensitive molecular diagnostics. The system can amplify target nucleic acids directly on the SERS surface, eliminating the need for post-amplification hybridization and Raman reporters. Clinical validation with COVID-19 samples yielded high sensitivity and specificity. The integration of a deep learning program further enhanced sensitivity and specificity to 100%.
Article
Spectroscopy
Tong Zhang, Shunhua Zhu, Jingjing Wang, Zhiying Liu, Mingxin Wang, Shibao Li, Qingli Huang
Summary: Fabricating sensitive, stable and low-cost nanomaterials for glucose detection is crucial for disease diagnosis and monitoring. In this study, a nanocomposite (polypyrrole bridging GO@Au@MnO2) was developed as a novel surface-enhanced Raman scattering (SERS) nanoprobe for quantitative glucose detection in trace serum.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Nanoscience & Nanotechnology
Yanlin Mi, Yinzhou Yan, Mengyuan Wang, Lixue Yang, Jing He, Yijian Jiang
Summary: Researchers have reported a novel hybrid SERS substrate composed of a large microsphere and a small microsphere array with Ag nanoparticles. The cascaded structure allows for increased molecular concentration, enhanced intensity of excitation light, and giant Raman enhancement.
Article
Physics, Multidisciplinary
Guanzhou Lin, Bingquan Yang, Chenglong Zhang, Jia Zhu, Yang Wang, Liye Li, Shisong Xiong, Jinwen Zhang, Zhimei Qi, Wengang Wu
Summary: In this study, a simple composite substrate combining a metal metasurface and silver nanoparticles was developed to enhance the performance of surface-enhanced Raman scattering (SERS) sensing. The composite substrate showed high enhancement factor, good stability, and reproducibility, enabling the detection of trace substances.
Review
Biotechnology & Applied Microbiology
Ting Lin, Ya-Li Song, Pu Kuang, Si Chen, Zhigang Mao, Ting-Ting Zeng
Summary: The optical technology of Surface-enhanced Raman scattering (SERS) plays a significant role in cancer diagnosis, with its performance being highly dependent on the properties and morphology of the substrate. SERS technology offers advantages of rapidity, high specificity, and high sensitivity.
Article
Engineering, Electrical & Electronic
Cheng-Yi Li, Sandy Huey-Jen Hsu, Cheng-Chung Chang, Gou-Jen Wang
Summary: The study introduced a novel method for detecting direct bilirubin by Raman spectroscopy using composite SERS chips, which significantly improved the detection limit, covered a wide detection range, and showed good reproducibility.
IEEE SENSORS JOURNAL
(2021)
Review
Chemistry, Physical
Ryan D. Norton, Hoa T. Phan, Stephanie N. Gibbons, Amanda J. Haes
Summary: This review discusses the recent advances in quantitative SERS detection, focusing on intermolecular interactions, surface selection rules, and target molecule solubility and accessibility. The impacts of surface selection rules and enhancement mechanisms on the activation and deactivation of normal Raman modes in SERS are explored. The review also summarizes the experimental conditions that can be used to tune the molecular affinity to and density near SERS substrates.
ANNUAL REVIEW OF PHYSICAL CHEMISTRY
(2022)
Article
Spectroscopy
Soogeun Kim, Young Jin Kim, Ayoung Bang, Wansun Kim, Samjin Choi, Hee Joo Lee
Summary: The study investigated the impact of Raman excitation wavelengths on the surface-enhanced Raman spectroscopy (SERS) for the identification of nontuberculous mycobacteria (NTM). By comparing SERS spectra with three commonly used excitation wavelengths, it was found that NTM species could be distinguished with statistical differences. Utilizing principal components-linear discriminant analysis and leave-one-out cross validation, the study achieved identification accuracies for six NTM species using aromatic amino acid biomarkers at different excitation wavelengths.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Review
Chemistry, Analytical
Francisco Pena-Pereira, Carlos Bendicho, Dragana Mutavdzic Pavlovic, Antonio Martin-Esteban, Myriam Diaz-Alvarez, Yuwei Pan, Jon Cooper, Zhugen Yang, Ivo Safarik, Kristyna Pospiskova, Marcela A. Segundo, Elefteria Psillakis
Summary: This paper reviews miniaturized analytical methods for the determination of contaminants of emerging concern (CECs) in environmental samples, covering the importance of monitoring CECs, downsized sample preparation methods, microextraction techniques, and miniaturized analytical flow techniques.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemistry & Molecular Biology
Jiabao Xu, Xiaofei Yi, Guilan Jin, Di Peng, Gaoya Fan, Xiaogang Xu, Xin Chen, Huabing Yin, Jonathan M. Cooper, Wei E. Huang
Summary: Accurate and rapid identification of infectious bacteria is crucial in medicine. Raman microspectroscopy shows potential in label-free identification at the single-cell level. In this study, machine learning methods and data processing techniques were used to achieve high-accuracy bacterial identification, opening up new possibilities for high-speed Raman microspectroscopic clinical diagnostics.
ACS CHEMICAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Gaolian Xu, Julien Reboud, Yunfei Guo, Hao Yang, Hongchen Gu, Chunhai Fan, Xiaohua Qian, Jonathan M. Cooper
Summary: Accelerating the design of nucleic acid amplification methods is challenging, but necessary for diagnosing diseases. This study presents a generalizable isothermal nucleic acid amplification methodology that can be applied to clinical settings, allowing for the detection of short miRNAs and difficult pathogenic targets.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Yang Liu, Filipe Pinto, Xinyi Wan, Zhugen Yang, Shuguang Peng, Mengxi Li, Jonathan M. Cooper, Zhen Xie, Christopher E. French, Baojun Wang
Summary: This study reveals the programmability of crRNA-tracrRNA hybridization in type II CRISPR systems. By reprogramming the crRNA-tracrRNA interactions, the researchers demonstrate the design of orthogonal cellular computing devices and the hijacking of endogenous small RNAs/mRNAs as crRNAs. Furthermore, they demonstrate the use of re-engineered gRNA pairings as RNA sensors for monitoring gene transcription or detecting SARS-CoV-2 RNA.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Yajuan Dong, Yongjuan Zhao, Shenwei Li, Zhenzhou Wan, Renfei Lu, Xianguang Yang, Guoying Yu, Julien Reboud, Jonathan M. Cooper, Zhengan Tian, Chiyu Zhang
Summary: This study reports a variant-tolerant diagnostic test for SARS-CoV-2 using loop-mediated isothermal nucleic acid amplification (LAMP) assay. The test shows excellent clinical performance and can detect a wide range of variants.
Article
Nanoscience & Nanotechnology
Han Cui, Andrew Glidle, Jonathan M. Cooper
Summary: This study demonstrates the use of stimulated Raman scattering technique to measure molecular diffusion in soft tissue samples, overcoming the issues of weak signal and fluorescence interference associated with spontaneous Raman spectroscopy. The results show that mass transport follows diffusion process within hydrogel matrices, but more complex mechanisms exist within tissues.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Physics, Applied
P. Yambangyang, R. Wilson, J. Reboud, J. M. Cooper, A. Demcenko
Summary: This study proposes a nondestructive nonlinear ultrasonic evaluation technique for detecting the physical aging of amorphous polymers in situ without needing to remove materials. The results show that the physical aging of the polymer can be described by the Arrhenius equation.
APPLIED PHYSICS LETTERS
(2022)
Article
Engineering, Biomedical
Yao Ge, Ahmad Taha, Syed Aziz Shah, Kia Dashtipour, Shuyuan Zhu, Jonathan Cooper, Qammer H. Abbasi, Muhammad Ali Imran
Summary: WiFi sensing has gained significant attention as a potential mechanism for remote monitoring of the aging population without deploying devices on users' bodies. It has the potential to detect critical events such as falls, sleep disturbances, and respiratory disorders. Unlike other sensing methods, WiFi technology is easy to implement and unobtrusive. This paper reviews the current state-of-the-art research on WiFi-based sensing and discusses its healthcare applications and open research challenges.
IEEE REVIEWS IN BIOMEDICAL ENGINEERING
(2023)
Correction
Multidisciplinary Sciences
Gaolian Xu, Hao Yang, Jiani Qiu, Julien Reboud, Linqing Zhen, Wei Ren, Hong Xu, Jonathan M. Cooper, Hongchen Gu
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Gaolian Xu, Hao Yang, Jiani Qiu, Julien Reboud, Linqing Zhen, Wei Ren, Hong Xu, Jonathan M. M. Cooper, Hongchen Gu
Summary: The authors propose a method called STEM-PCR for rapid and facile detection of specific nucleic acid modifications. This method utilizes a simple and widely accessible PCR-based workflow, and exhibits sensitivity 20 times greater than current gold-standard techniques by using a bisulfite-free process. They successfully demonstrate the clinical applicability of this method by detecting methylation of SEPTIN9 and SFRP2 genes, which are key biomarkers in tumor prognosis and diagnosis.
NATURE COMMUNICATIONS
(2023)
Article
Engineering, Electrical & Electronic
Yao Ge, Wenda Li, Muhammad Farooq, Adnan Qayyum, Jingyan Wang, Zikang Chen, Jonathan Cooper, Muhammad Ali Imran, Qammer H. Abbasi
Summary: The LoGait system utilizes LoRa signals to recognize gait in indoor environments, overcoming challenges such as video quality, occlusion, and privacy concerns. It enables a larger sensing range of gait recognition compared to WiFi-based systems.
IEEE SENSORS JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Rodrigo Ibarra-Chavez, Julien Reboud, Jose R. Penades, Jonathan M. Cooper
Summary: Phage-inducible chromosomal islands (PICIs) are genetic constructs that repurpose phage components to mobilize and spread. They have been used as antibacterial drones and a user-friendly diagnostic system based on PICI transduction has been developed for selective enrichment and detection of Escherichia coli and Staphylococcus aureus. This approach enables visual sensing of viable pathogens only, extending diagnostic sensing mechanisms beyond conventional strategies.
Article
Biochemical Research Methods
Yunus Aslan, Olivia McGleish, Julien Reboud, Jonathan M. Cooper
Summary: This study presents an alignment-free spatial hydrophilic PDMS patterning technique for the generation of double emulsion droplets. The method successfully produces reliable and reproducible water-in-oil-in-water droplet emulsions without the need for complex processing steps. It can be applied to various fields, further expanding the applications of microfluidic techniques.
Article
Nanoscience & Nanotechnology
Han Cui, Andrew Glidle, Jonathan M. Cooper
Summary: This study investigates the molecular diffusion across biomaterial interfaces using stimulated Raman scattering technique. The results show that background fluorescence can interfere with the signal when using spontaneous Raman spectroscopy. By using stimulated Raman scattering, measurements with high signal-to-noise ratios and minimal fluorescence interference can be obtained. The study also reveals the existence of more complex diffusion mechanisms in tissues.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Proceedings Paper
Optics
Victor J. Ochoa-Gutierrez, Mauro Pazmino Betancourth, Julien Reboud, Andrew R. Harvey, Jonathan M. Cooper
Summary: Research on the use of Mid-IR in developing future point of care tools aims to monitor health proxies in blood samples. FTIR analysis of heme groups was used to find spectral signatures for quantifying the redox state of haemoglobin. ATR spectroscopy revealed spectral differences between HbMet and Hb/HbO(2) in specific regions, showing potential for expanding optical tools in medical monitoring and diagnosis.
FRONTIERS IN BIOPHOTONICS AND IMAGING
(2021)