Thermal Denaturation Assays in Chemical Biology

Comparison of fluorescence and light scattering based methods to assess formation and stability of protein–protein complexes

(2011) Jolanta Kopec et al.   JOURNAL OF STRUCTURAL BIOLOGY

A chemical probe selectively inhibits G9a and GLP methyltransferase activity in cells

(2011) Masoud Vedadi et al.   Nature Chemical Biology

Thermodynamic Analysis of Ligand-Induced Changes in Protein Thermal Unfolding Applied to High-Throughput Determination of Ligand Affinities with Extrinsic Fluorescent Dyes

(2010) Curtis J. Layton et al.   BIOCHEMISTRY

Differential scanning calorimetry and fluorescence study of lactoperoxidase as a function of guanidinium–HCl, urea, and pH

(2010) Bogumil Zelent et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Protein Lysine Methyltransferase G9a Inhibitors: Design, Synthesis, and Structure Activity Relationships of 2,4-Diamino-7-aminoalkoxy-quinazolines.

(2010) Feng Liu et al.   JOURNAL OF MEDICINAL CHEMISTRY

Screening of Monoclonal Antibody Formulations Based on High-Throughput Thermostability and Viscosity Measurements: Design of Experiment and Statistical Analysis

(2010) Feng He et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Formulation Development of Therapeutic Monoclonal Antibodies Using High-Throughput Fluorescence and Static Light Scattering Techniques: Role of Conformational and Colloidal Stability

(2010) Deborah S. Goldberg et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Biophysical characterization of recombinant proteins: A key to higher structural genomics success

(2010) Masoud Vedadi et al.   JOURNAL OF STRUCTURAL BIOLOGY

Thermolability of mutant MMACHC protein in the vitamin B12-responsive cblC disorder

(2010) D.S. Froese et al.   MOLECULAR GENETICS AND METABOLISM

A survey of proteins encoded by non-synonymous single nucleotide polymorphisms reveals a significant fraction with altered stability and activity

(2009) Abdellah Allali-Hassani et al.   BIOCHEMICAL JOURNAL

Effect of Metal Ion on the Structural Stability of Tumour Suppressor Protein p53 DNA-Binding Domain

(2009) Y. Xue et al.   JOURNAL OF BIOCHEMISTRY

Discovery of a 2,4-Diamino-7-aminoalkoxyquinazoline as a Potent and Selective Inhibitor of Histone Lysine Methyltransferase G9a

(2009) Feng Liu et al.   JOURNAL OF MEDICINAL CHEMISTRY

Stability of two β-tropomyosin isoforms: effects of mutation Arg91Gly

(2009) Ilya Nevzorov et al.   JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY

High throughput thermostability screening of monoclonal antibody formulations

(2009) Feng He et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

High-Throughput Thermal Scanning: A General, Rapid Dye-Binding Thermal Shift Screen for Protein Engineering

(2009) Jason J. Lavinder et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Crystallization and preliminary X-ray analysis of CrgA, a LysR-type transcriptional regulator from pathogenicNeisseria meningitidisMC58

(2008) Sarah Sainsbury et al.   Acta Crystallographica Section F-Structural Biology and Crystallization Communications

ATLAS—A High-Throughput Affinity-Based Screening Technology for Soluble Proteins: Technology Application Using p38 MAP Kinase

(2008) Rupal Patel et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

Identification of Ligand Binding by Protein Stabilization: Comparison of ATLAS with Biophysical and Enzymatic Methods

(2008) Peggy A. Thompson et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

Functional Assignment of Solute-Binding Proteins of ABC Transporters Using a Fluorescence-Based Thermal Shift Assay†

(2008) Sarah E. Giuliani et al.   BIOCHEMISTRY

Thermodynamic characterization of the palm tree Roystonea regia peroxidase stability

(2008) Laura S. Zamorano et al.   BIOCHIMIE

Thermal inactivation, denaturation and aggregation of mitochondrial aspartate aminotransferase

(2008) Nikolay V. Golub et al.   BIOPHYSICAL CHEMISTRY

A Quantitative Model of Thermal Stabilization and Destabilization of Proteins by Ligands

(2008) Piotras Cimmperman et al.   BIOPHYSICAL JOURNAL

Crystal and solution structure, stability and post-translational modifications of collapsin response mediator protein 2

(2008) Viivi Majava et al.   FEBS Journal

Pyruvate kinase M2 is a phosphotyrosine-binding protein

(2008) Heather R. Christofk et al.   NATURE

The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth

(2008) Heather R. Christofk et al.   NATURE

Demonstration of MEMS-based differential scanning calorimetry for determining thermodynamic properties of biomolecules

(2008) Li Wang et al.   SENSORS AND ACTUATORS B-CHEMICAL

Microscale Fluorescent Thermal Stability Assay for Membrane Proteins

(2008) Alexander I. Alexandrov et al.   STRUCTURE

The Threat of Instability: Neurodegeneration Predicted by Protein Destabilization and Aggregation Propensity

(2008) Elizabeth M Meiering   PLOS BIOLOGY