Article
Biochemical Research Methods
Madeline E. McCarthy, William B. Dodd, Xiaoming Lu, Daniel J. Pritko, Nishi D. Patel, Charlotte V. Haskell, Hugo Sanabria, Mark A. Blenner, Marc R. Birtwistle
Summary: We propose a theory for high-throughput genetic interaction screens, which extends the Multiplexing using Spectral Imaging and Combinatorics (MuSIC) approach to create 100,000 spectrally unique MuSIC barcodes. Experimental testing of this theory could revolutionize genetic perturbation technology and knowledge of genetic function. Additionally, a genome-scale spectral barcode library could have widespread applications in genetic screening and lineage tracing.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Engineering, Environmental
Ling Tan, Meizi Liu, Lixian Wang, Guoping Zhao, Yanfei Zhang
Summary: A novel semi-quantitative method using flow cytometry analysis of side scatter intensity has been proposed to measure the adsorption capacity of individual cells to Pd(II). This method enables high-throughput screening of specific peptides/proteins with high Pd(II) affinity.
JOURNAL OF HAZARDOUS MATERIALS
(2024)
Review
Pharmacology & Pharmacy
Chelsea B. Pratt, Matt Alexander
Summary: The development of CAR-T cells is crucial for the success of adoptive T-cell immunotherapy. While the process of producing autologous CAR-T cells is time-consuming and expensive, the exploration of cost-effective allogeneic CAR-T cells is essential. High-throughput tools are used to monitor the entire process of CAR-T cell design, testing, manufacturing, and patient monitoring to ensure safety and efficacy.
DRUG DISCOVERY TODAY
(2022)
Article
Dermatology
Valentin Parat, Brigitte Onteniente, Julien Maruotti
Summary: This study describes a simple assay using induced pluripotent stem cell-derived melanocytes and high-throughput flow cytometry to identify the effect of pigment regulating agents on melanin content. The assay is validated using 80 small molecules and may serve as a useful tool to identify modulators of melanogenesis in human melanocytes.
EXPERIMENTAL DERMATOLOGY
(2021)
Review
Chemistry, Analytical
Majood Haddad, Alex N. Frickenstein, Stefan Wilhelm
Summary: Understanding nanoparticle-cell interactions at single-nanoparticle and single-cell resolutions is crucial to improving the design of next-generation nanoparticles for safer, more effective, and more efficient applications in nanomedicine. This review focuses on recent advances in the continuous high-throughput analysis of nanoparticle-cell interactions at the single-cell level, discussing methods such as flow cytometry and inductively coupled plasma mass spectrometry, as well as their intersection in mass cytometry. The review also explores the challenges and opportunities in current single-cell analysis approaches and proposes directions for innovation in high-throughput analysis of nanoparticle-cell interactions.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Christian Sudfeld, Michal Hubacek, Sarah D'Adamo, Rene H. Wijffels, Maria J. Barbosa
Summary: An optimized single cell lipid screening procedure for Nannochloropsis oceanica has been developed in this study, with different staining conditions identified for neutral lipids in exponentially growing cultures and lipid-overproducing cultures. The use of DMSO for cell permeabilization and varying concentrations of BODIPY for staining were highlighted, along with the effects on cell viability, paving the way for a quantitative prediction of cellular neutral lipids.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Nanoscience & Nanotechnology
Xiaopeng Yang, Kun Wang, Peng Huang, Yimin Guan, Demeng Liu
Summary: A novel impedance-based cell counting chip based on thermal bubble drive was designed in this study, which provides a driving force for cells through a combination of thermal bubble nozzles and microfluidic channels. The chip achieves high throughput detection of 45,000 beads/s under extreme conditions, with a fourfold difference in detection voltage obtained.
Article
Chemistry, Analytical
Gerjen H. Tinnevelt, Kristiaan Wouters, Geert J. Postma, Rita Folcarelli, Jeroen J. Jansen
Summary: White blood cells play a crucial role in protecting the body from diseases, but can also be related to chronic inflammation, auto immune diseases, or leukemia. High-throughput analytical instruments have been developed to measure multiple proteins on millions of single cells to study the identity and function of different white blood cell types. Multivariate statistics are essential for fully extracting the information-rich biochemistry data. The process involves analyzing the study design, formulating a research question, preparing the data, converting single cells into a cellular distribution, and using clustering methods or models for analysis to differentiate cell (sub)types between groups.
ANALYTICA CHIMICA ACTA
(2021)
Review
Chemistry, Analytical
Zheng Zhou, Yao Chen, Shu Zhu, Linbo Liu, Zhonghua Ni, Nan Xiang
Summary: Inertial microfluidics, proposed in 2007, has been extensively studied and applied in the biomedical field, offering advantages such as high throughput, simplicity, and low cost. This technology is promising for rapid sample processing, cell analysis, and detection, playing a crucial role in cellular sample pretreatment.
Article
Materials Science, Multidisciplinary
Matthew Lindley, Julia Gala de Pablo, Jorgen Walker Peterson, Akihiro Isozaki, Kotaro Hiramatsu, Keisuke Goda
Summary: Cell sorting is an important tool in biological research and medicine. Raman-activated cell sorting (RACS) has gained attention for its ability to discriminate cells based on their intracellular chemical content without the need for labeling. However, the widespread use of RACS has been limited by a trade-off between throughput and measurement bandwidth. In this study, this trade-off is overcome and broadband RACS in the fingerprint region with a record high throughput is demonstrated. Real-time label-free sorting of microalgal cells based on their accumulation of carotenoids and polysaccharide granules is also showcased.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Physics, Applied
Julie Martin-Wortham, Steffen M. Recktenwald, Marcelle G. M. Lopes, Lars Kaestner, Christian Wagner, Stephan Quint
Summary: The proposed method combines spatial optical modulation and deep learning to detect cells label-free at high throughput while providing their brightfield images. It requires minimal optical components, suitable for lab-on-chip devices, and has been benchmarked with a dataset of red blood cell images.
APPLIED PHYSICS LETTERS
(2021)
Article
Cell Biology
Gesa Witt, Oliver Keminer, Jennifer Leu, Rashmi Tandon, Ina Meiser, Anne Willing, Ingo Winschel, Jana-Christin Abt, Bjoern Braendl, Isabelle Sebastien, Manuel A. Friese, Franz-Josef Mueller, Julia C. Neubauer, Carsten Claussen, Heiko Zimmermann, Philip Gribbon, Ole Pless
Summary: This study highlights the significance of the embryonic stem cell test (EST) and its potential applications in pharmaceutical development. Several improvements to the workflow have been introduced to enhance sensitivity, speed, and reproducibility, paving the way for predicting in vivo developmental toxicity from in vitro data.
CELL BIOLOGY AND TOXICOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Zi-Xu Liu, Si-Ling Huang, Jin Hou, Xue-Ping Guo, Feng-Shan Wang, Ju-Zheng Sheng
Summary: Valuable polysaccharides are typically produced through fermentation using wild-type or metabolically-engineered microbial strains, with high-throughput screening techniques being used to identify cells with high levels of polysaccharide synthesis. Strategies such as fluorescence labeling, fluorescence quenching, and biosensors are effective for cell sorting in industrial strains and pathway optimization for polysaccharide synthesis. Additionally, high-throughput screening using individual whole cells is critical in the discovery and evolution of enzymes involved in polysaccharide biosynthesis, with these enzymes and their mutants being valuable catalysts for saccharide synthesis.
MICROBIAL CELL FACTORIES
(2021)
Article
Biochemistry & Molecular Biology
Jorge A. Lerma Romero, Christian Meyners, Andreas Christmann, Lisa M. Reinbold, Anna Charalampidou, Felix Hausch, Harald Kolmar
Summary: This article describes a protein engineering strategy that identified variants with higher affinity binding of conformation-specific FKBP51 selective inhibitors. These variants will provide a better understanding of the conformational flexibility of the FKBP51 binding pocket and may enable the isolation of new selective ligands.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemical Research Methods
Kunpeng Cai, Shruti Mankar, Taiga Ajiri, Kentaro Shirai, Tasuku Yotoriyama
Summary: The study introduces a fully integrated high-throughput microfluidic circulatory fluorescence-activated cell sorting system capable of significantly increasing the purity of rare cells in a short period of time and effectively enriching different types of cells. Through a sequential sorting process and efficient contamination-free design, effective enrichment of clinical rare cells has been achieved.