Article
Medicine, General & Internal
Naidan Zhang, Chaixia Ji, Xiao Bao, Chengliang Yuan
Summary: This study investigated the clinical characteristics and significance of ANA cytoplasmic patterns in AAV patients from Southwest China, and found that these patterns were associated with the clinical manifestations and renal involvement in AAV.
Article
Medicine, General & Internal
Nobuhide Hayashi, Kenichi Uto, Akiko Imanishi, Daisuke Sugiyama, Akio Morinobu, Jun Saegusa
Summary: The prevalence of anti-DFS70 autoantibodies in healthy individuals and patients with autoimmune rheumatic diseases in Japan is similar, but higher than in other Asian regions. The presence of anti-DFS70 antibodies complicates the differential diagnosis of autoimmune rheumatic diseases through antinuclear antibody screening.
Article
Immunology
Monica Jesus Silva, Alessandra Dellavance, Danielle Cristiane Baldo, Silvia Helena Rodrigues, Marcelle Grecco, Monica Simon Prado, Renan Agustinelli, Luis Eduardo Coelho Andrade
Summary: Interkit nonreproducibility in HEp-2/IFA is common and occurs most frequently with weakly reactive samples. Efforts should be made to promote harmonization of the properties and performance of HEp-2/IFA commercial kits through international initiatives involving the in vitro diagnostic industry.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Rheumatology
Juan Irure-Ventura, Carmen Rodriguez, Esther Vergara-Prieto, Maria Luisa Vargas, Bibiana Quirant, Aurora Jurado, Luis Fernandez-Pereira, Eva Martinez-Caceres, Miriam San Jose, Marcos Lopez-Hoyos
Summary: The study retrospectively collected clinical data from patients with confirmed uncommon HEp-2 IIFA patterns to search for an associated clinical condition. It found that nuclear patterns were more common in SLE and SS, while cytoplasmic patterns were higher in SSc and inflammatory myositis. Detection of infrequent ANA without disease-associated specificities could be a unique finding that indicates the presence of an autoimmune disease.
Article
Hematology
Jing Liu, Yuelun Zhang, Zhuo Li, Ji Li, Lejia Zhang, Yuqing Song, Zichao Lyu, Changyan Wang, Lijuan Gou, Meiying Quan, Juan Xiao, Hongmei Song
Summary: A single-center retrospective cohort study conducted in China found that antinuclear antibody (ANA) is positive in children with primary immune thrombocytopenia (ITP). However, the effect of ANA titre on the outcomes of children with primary ITP remains unclear.
BRITISH JOURNAL OF HAEMATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Han-Hua Yu, Pao-Feng Hsieh, Szu-Wei Huang, Tien-Ming Chan, Pao-Lien Tai, Shih-Ting Yang, Kuang-Hui Yu, Marcella Prete
Summary: This study developed a flowchart to discriminate between AC-1 and AC-2 ANA patterns and re-evaluated ANA samples according to this flowchart. The results showed that AC-2 was highly capable of detecting anti-DFS70 antibodies, while AC-1 showed increased ability to detect anti-nucleosome/histone/dsDNA antibodies. Furthermore, more than half of the AC-2 samples had specific autoantibodies other than anti-DFS70.
Article
Rheumatology
Carolina Munoz-Grajales, Stephenie D. Prokopec, Sindhu R. Johnson, Zahi Touma, Zareen Ahmad, Dennisse Bonilla, Linda Hiraki, Arthur Bookman, Paul C. Boutros, Andrzej Chruscinski, Joan Wither
Summary: By investigating autoantibodies in ANA(+) individuals, we found that early SARD patients have high levels of autoantibodies, reacting to many self-antigens that are not detected by current screening techniques. Only anti-Ro52 antibodies were able to predict symptomatic progression. Surprisingly, the levels of autoantibodies remained stable over time.
Article
Medicine, Research & Experimental
Yun Xiao, Yiqiang Lin, Yan Zhang, Jiajia Wang, Yanli Zeng
Summary: This study found that a decrease in serum anti-nuclear antibody (ANA) levels in systemic autoimmune rheumatic diseases (SARDs) after 12 months of treatment is associated with patient age and baseline ANA titers.
CLINICAL AND EXPERIMENTAL MEDICINE
(2022)
Article
Pediatrics
Min Ji Park, Hae Min Jang, Dongsub Kim, Dong Il Won, Hee Sun Baek, Min Hyun Cho
Summary: This study aimed to evaluate the clinical significance of antinuclear antibody (ANA) in the diagnosis of systemic autoimmune diseases (AiD) in pediatric patients by considering their titers and patterns. Among the 2442 children tested for ANA, 19.4% were positive and 7.0% were diagnosed with significant AiD. The positive predictive value (PPV) for significant AiD was high when the ANA titer was >= 1:640, while the PPV for dense fine speckled (DFS) pattern was significantly lower compared to speckled and homogenous patterns.
CLINICAL PEDIATRICS
(2023)
Article
Oncology
Rong Wang, Huijuan Zhao, Yang Liu, Bing Kang, Jun Cai
Summary: This study found that in adult patients with leukemia, ANAs with a nucleolar pattern were associated with a two-fold increased risk of death compared to patients with negative antibodies, independent of various factors. This association was more significant in older patients (>=60 years) and those treated with tyrosine kinase inhibitors or chemotherapy. Patients with a nucleolar pattern had significantly shorter survival than those with a non-nucleolar pattern or without ANA.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemical Research Methods
Kechi Fang, Chuan Li, Jing Wang
Summary: Immunofluorescence patterns of anti-nuclear antibodies (ANAs) on human epithelial cell (HEp-2) substrates are important biomarkers for the diagnosis of autoimmune diseases. In this study, an automatic immunofluorescence pattern classification framework for HEp-2 specimen images was developed, achieving higher accuracy than human examiners on the same test dataset.
BRIEFINGS IN BIOINFORMATICS
(2023)
Article
Cell Biology
Jonathan D. Herman, Caroline Atyeo, Yonatan Zur, Claire E. Cook, Naomi J. Patel, Kathleen M. Vanni, Emily N. Kowalski, Grace Qian, Shruthi Srivatsan, Nancy A. Shadick, Deepak A. Rao, Benjamin Kellman, Colin J. Mann, Douglas Lauffenburger, Zachary S. Wallace, Jeffrey A. Sparks, Galit Alter
Summary: Alterations in humoral immunity, including weaker binding of anti-SARS-CoV-2 antibodies and stronger binding of antibodies against endemic coronavirus OC43, were observed in individuals with PASC. Previous coronavirus imprinting may serve as a potential marker for the development of PASC.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Article
Immunology
Jingjing Meng, Rong Wang, Yueming Luo, Siting Li, Yina Bai, Ning Song, Mengtao Li, Xiaofeng Zeng, Chaojun Hu
Summary: DFS pattern has a low prevalence in Chinese patients, but it is more common in patients with dermatosis and SARDs. Higher titres of the DFS pattern are associated with an increased risk of SARDs. The DFS pattern cannot be used as an exclusion criterion for SARDs diagnosis.
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
(2023)
Article
Medicine, General & Internal
Chuiwen Deng, Anqi Wang, Chaojun Hu, Wen Zhang, Xiaofeng Zeng, Yunyun Fei
Summary: Based on a retrospective study, the prevalence of the dense fine speckled (DFS) immunofluorescence staining pattern was found to be 1.1% in a non-selective population. This pattern was associated with various pathological conditions and may indicate localized inflammation within certain organs or tissues, particularly the skin.
FRONTIERS IN MEDICINE
(2022)
Article
Rheumatology
Annemarie Lyng Lyng Svensson, Hanne-Dorthe Emborg, Lars Erik Bartels, Torkell Ellingsen, Thomas Adelsten, Rene Cordtz, Lene Dreyer, Niels Obel
Summary: In a Danish study, patients with inflammatory rheumatic diseases (IRD) were found to have a similar risk of contracting SARS-CoV-2 compared to the general population, but a significantly higher risk of hospitalization, severe COVID-19, requiring assisted ventilation, and death, especially in patients with comorbidities.
ANNALS OF THE RHEUMATIC DISEASES
(2023)
Editorial Material
Rheumatology
Luis Eduardo Coelho Andrade, Jan Damoiseaux, Edward K. Chan
ANNALS OF THE RHEUMATIC DISEASES
(2020)
Article
Medical Laboratory Technology
Monica Simon Prado, Alessandra Dellavance, Silvia Helena Rodrigues, Valdecir Marvulle, Luis Eduardo Coelho Andrade
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2020)
Article
Rheumatology
Anna Larissa Faria Janes, Miriam Fang Castro, Anne Elizabeth Diniz Arraes, Bruna Savioli, Emilia Inoue Sato, Alexandre W. S. de Souza
RHEUMATOLOGY INTERNATIONAL
(2020)
Article
Rheumatology
Alex Magno Coelho Horimoto, Laize Guerreiro de Jesus, Albert Schiaveto de Souza, Silvia Helena Rodrigues, Cristiane Kayser
ADVANCES IN RHEUMATOLOGY
(2020)
Article
Genetics & Heredity
Caio Robledo D'Angioli Costa Quaio, Caroline Monaco Moreira, Gil Monteiro Novo-Filho, Patricia Rossi Sacramento-Bobotis, Michele Groenner Penna, Sandro Felix Perazzio, Aurelio Pimenta Dutra, Rafael Alves da Silva, Monize Nakamoto Provisor Santos, Vanessa Yurie Nozaki de Arruda, Vanessa Galdeno Freitas, Vinicius Ceola Pereira, Maria Carolina Pintao, Alexandre Ricardo dos Santos Fornari, Ana Ligia Buzolin, Andre Yuji Oku, Matheus Burger, Rodrigo Fernandes Ramalho, David Santos Marco Antonio, Elisa Napolitano e Ferreira, Otavio Jose Eulalio Pereira, Vanessa Dionisio Cantagalli, Ana Carolina Gomes Trindade, Rafaela Rogerio Floriano de Sousa, Cintia Reys Furuzawa, Fernanda Verzini, Shirley Dezan Matalhana, Naiade Romano, Daniele Paixao, Caroline Olivati, Gustavo Marquezani Spolador, Gustavo Arantes Rosa Maciel, Viviane Zorzanelli Rocha, Javier Miguelez, Mario Henrique Burlacchini de Carvalho, Alexandre Wagner Silva de Souza, Luis Eduardo Coelho Andrade, Maria de Lourdes Chauffaille, Aline dos Santos Borgo Perazzio, Ana Lucia Pereira Monteiro Catelani, Miguel Mitne-Neto, Chong Ae Kim, Wagner Antonio da Rosa Baratela
AMERICAN JOURNAL OF MEDICAL GENETICS PART C-SEMINARS IN MEDICAL GENETICS
(2020)
Article
Rheumatology
Alexandre W. S. de Souza, Eduarda Bonelli Zarur, Victor R. Pimentel-Quirozz, Alfredo Sanchez-Torres, Manuel F. Ugarte-Gil, Emilia Inoue Sato
Summary: This study compared the prevalence of systemic vasculitides between two vasculitis referral centers in Brazil and Peru. The study found that the frequency of Behget's disease, Takayasu arteritis, and giant cell arteritis was higher in the Brazilian center, while the frequency of microscopic polyangiitis and renal-limited vasculitis was higher in the Peruvian center.
CLINICAL RHEUMATOLOGY
(2022)
Article
Immunology
Monica Jesus Silva, Alessandra Dellavance, Danielle Cristiane Baldo, Silvia Helena Rodrigues, Marcelle Grecco, Monica Simon Prado, Renan Agustinelli, Luis Eduardo Coelho Andrade
Summary: Interkit nonreproducibility in HEp-2/IFA is common and occurs most frequently with weakly reactive samples. Efforts should be made to promote harmonization of the properties and performance of HEp-2/IFA commercial kits through international initiatives involving the in vitro diagnostic industry.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medical Laboratory Technology
Edward K. L. Chan, Carlos A. von Muhlen, Marvin J. Fritzler, Jan Damoiseaux, Maria Infantino, Werner Klotz, Minoru Satoh, Lucile Musset, Ignacio Garcia-De la Torre, Orlando Gabriel Carballo, Manfred Herold, Wilson de Melo Cruvinel, Tsuneyo Mimori, Luis E. C. Andrade
Summary: The establishment of the International Consensus on ANA Patterns (ICAP) was widely welcomed by the medical community and has led to improvements in the classification of HEp-2 IFA patterns. This article summarizes the discussions and current plans from the 6th ICAP Workshop.
JOURNAL OF APPLIED LABORATORY MEDICINE
(2022)
Correction
Medical Laboratory Technology
Edward K. L. Chan, Carlos A. von Muhlen, Marvin J. Fritzler, Jan Damoiseaux, Maria Infantino, Werner Klotz, Minoru Satoh, Lucile Musset, Ignacio Garcia-De La Torre, Orlando Gabriel Carballo, Manfred Herold, Wilson de Melo Cruvinel, Tsuneyo Mimori, Karsten Conrad, Luis E. C. Andrade
JOURNAL OF APPLIED LABORATORY MEDICINE
(2023)
Review
Rheumatology
Priscila Dias Cardoso Ribeiro, Emilia Inoue Sato
Summary: This review focuses on silicone-induced autoimmune/autoinflammatory syndrome induced by adjuvants (ASIA). The use of silicone breast implants (SBI) has increased worldwide, raising concerns about their potential effects on the immune system, particularly in individuals with pre-existing immune dysregulation. Despite epidemiological studies, the association between SBI and autoimmune/rheumatic disorders remains inconclusive. While explantation of silicone breast implants improves unspecific symptoms in the majority of patients with silicone-induced ASIA, the outcome appears to be different in patients with definitive autoimmune rheumatic disease (AIRD). Prospective studies evaluating the risk of flares after SBI in patients with AIRD are lacking. It is important to discuss the risk of ASIA with patients with AIRD and consider long-term safety and implant-related outcomes on a case-by-case basis for shared decision-making between patients and physicians.
CLINICAL RHEUMATOLOGY
(2022)
Article
Immunology
Gerson Dierley Keppeke, Minoru Satoh, Cristiane Kayser, Pedro Matos, Tomoko Hasegawa, Shin Tanaka, Larissa Diogenes, Rogerio Quintiliano Amaral, Silvia Helena Rodrigues, Luis Eduardo Coelho Andrade
Summary: Anti-fibrillarin autoantibodies are useful for the diagnosis and prognosis of systemic sclerosis. A reliable cell-based anti-fibrillarin assay was developed and found to be comparable to the gold standard immunoprecipitation. Positive anti-fibrillarin results were associated with increased occurrence of certain disease phenotypes.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biology
E. B. Zarur, S. H. Rodrigues, O. Ambrogini, M. L. G. Ferraz, G. Mastroianni-Kirsztajn, L. E. C. Andrade, A. W. S. de Souza
Summary: This study assessed the impact of improvements in the IIF-ANCA technique on the positivity rate of ANCA tests. The results showed that modifications in the IIF-ANCA protocol led to a significant improvement in its positivity rate and titers.
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
(2023)
Article
Immunology
Luis Eduardo C. Andrade, Jan Damoiseaux, Diego Vergani, Marvin J. Fritzler
Summary: The classification and diagnosis of systemic autoimmune diseases rely on a set of criteria that include clinical, laboratory, imaging, and pathology factors. Autoantibodies play a crucial role in the classification criteria of many autoimmune diseases. HEp-2 indirect immunofluorescence assay is capable of detecting a wide range of autoantibodies. A positive HEp-2 IFA test can indicate the most likely autoantibody specificities in the sample. This paper suggests that the classification and diagnostic criteria for certain autoimmune diseases could be improved by modifying the accepted staining patterns for each disease based on relevant autoantibody specificities.
JOURNAL OF TRANSLATIONAL AUTOIMMUNITY
(2022)
Article
Rheumatology
Natalia Regine de Franca, Henri Andre Menard, Maximilien Lora, Zhijie Zhou, Joyce Rauch, Carol Hitchon, Luis Eduardo Coelho Andrade, Ines Colmegna
Summary: In this study, ECV304 cells were found to have self-citrullination capacity and novel cit-target proteins were identified in the autocitrullinated products immunoprecipitated by sera from RA patients. The auto-cit-prot-ECV304 ELISA showed high sensitivity and specificity for RA diagnosis.
ARTHRITIS RESEARCH & THERAPY
(2022)