Article
Biochemical Research Methods
Daniela Ligi, Rosanna Maniscalco, Ferdinando Mannello
JOURNAL OF PROTEOME RESEARCH
(2020)
Review
Medicine, General & Internal
Joseph D. Raffetto, Daniela Ligi, Rosanna Maniscalco, Raouf A. Khalil, Ferdinando Mannello
Summary: Venous leg ulcers (VLUs) are a common type of ulcer in the lower extremity with significant socioeconomic and psychological impact. The pathogenesis involves factors such as endothelial cell injury and inflammatory responses, while treatment primarily includes compression therapy and endovenous ablation. Proper wound care and therapy usually result in healing within 6 months.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Peripheral Vascular Disease
Claudio Allegra, Giacomo Failla, Luca Costanzo, Ferdinando Mannello, Francesco Montella, Pier L. Antignani
Summary: The paper reviewed literature related to COVID-19 therapy and proposed outpatient treatment protocols according to age and comorbidity, aiming to reduce the virus's impact on cells and inflammation burden.
INTERNATIONAL ANGIOLOGY
(2021)
Review
Medicine, General & Internal
Bahare Fazeli, Daniela Ligi, Shayan Keramat, Rosanna Maniscalco, Hiva Sharebiani, Ferdinando Mannello
Summary: Diagnosis of thromboangiitis obliterans remains challenging, with no specific marker for differentiating it from atherosclerosis or vasculitis. Common biomarkers such as pro-inflammatory cytokines and oxidative stress are shared among the three diseases, but each disease occurs through a different pathway.
Editorial Material
Medicine, General & Internal
Ferdinando Mannello, Mario Plebani
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Medicine, General & Internal
Daniela Ligi, Rosanna Maniscalco, Mario Plebani, Giuseppe Lippi, Ferdinando Mannello
Summary: Several studies have shown the relationship between inflammation, thrombosis, multi-organ failures, and SARS-CoV-2 infection. Increased levels of histones have been associated with COVID-19, increasing the risk of heart attack and stroke. Therefore, histones can be used as diagnostic biomarkers for COVID-19 and as predictive laboratory tools for targeted therapies.
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Medical Laboratory Technology
Daniela Ligi, Rosaria Vincenza Giglio, Brandon M. Henry, Giuseppe Lippi, Marcello Ciaccio, Mario Plebani, Ferdinando Mannello
Summary: A literature review found an association between increased circulating histone levels in COVID-19 patients and disease severity/mortality. Monitoring blood histone concentration may be useful for early identification of unfavorable COVID-19 progression.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Review
Medical Laboratory Technology
Daniela Ligi, Bruna Lo Sasso, Brandon M. Henry, Marcello Ciaccio, Giuseppe Lippi, Mario Plebani, Ferdinando Mannello
Summary: SARS-CoV-2 infection is characterized by systemic and organ hyper-thromboinflammation, with monocytes playing a crucial role. Elevated monocyte distribution width (MDW) levels are associated with unfavorable outcomes in COVID-19 patients, indicating the potential of MDW as a useful diagnostic and prognostic marker. Monitoring MDW levels could aid in identifying high-risk patients and assessing disease progression and therapeutic efficacy.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Medical Laboratory Technology
Daniela Ligi, Bruna Lo Sasso, Chiara Della Franca, Rosaria Vincenza Giglio, Luisa Agnello, Marcello Ciaccio, Ferdinando Mannello
Summary: The study investigated the role of extracellular histones on monocyte distribution width (MDW) and their effect on the release of cytokines by blood cells. The results showed that extracellular histones can induce changes in MDW and promote heterogeneity in monocytes. They also lead to the release of cytokines, especially G-CSF, IL-1 beta, IL-6, MIP-1 beta, and IL-8. These findings may have significant implications for predicting the risk of severe outcomes.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Letter
Medical Laboratory Technology
Rosaria Vincenza Giglio, Daniela Ligi, Chiara Della Franca, Bruna Lo Sasso, Julia Zulema Rivas, Luisa Agnello, Ferdinando Mannello, Marcello Ciaccio
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Medicine, General & Internal
Rosanna Maniscalco, Giorgina Mangano, Alessandra Capezzone de Joannon, Matteo Vergassola, Sara Zucchi, Ferdinando Mannello, Lorella Ragni
Summary: This study evaluated the in vitro activity of a sodium hypochlorite solution on the release of matrix metalloproteinases (MMPs). The results showed that the solution reduced the activity of MMP-9 and its activated form, and also had an impact on the release of other MMPs. Additionally, the solution had a slight effect on wound healing.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Michela Bruschi, Federica Biancucci, Sofia Masini, Francesco Piacente, Daniela Ligi, Francesca Bartoccini, Antonella Antonelli, Ferdinando Mannello, Santina Bruzzone, Michele Menotta, Alessandra Fraternale, Mauro Magnani
Summary: Reduction in oxygen levels is a key feature in the physiology of the bone marrow (BM) niche where hematopoiesis occurs. Antioxidant molecules can modulate the redox status of endothelial cells (ECs) in a hypoxic environment, promoting hematopoietic stem cell (HSC) maintenance. In this study, HUVEC cells exposed to low oxygen were treated with I-152, which increased glutathione levels and influenced the metabolic profiles interconnected with the glutathione system. Gene and protein expression analyses showed modulation of redox-related genes and upregulation of antioxidants. I-152 also reduced oxidative stress and downregulated certain cytokines in hypoxic ECs, potentially creating an in vitro BM niche conducive to functional HSC maintenance.
Review
Medical Laboratory Technology
Daniela Ligi, Chiara Della Franca, Kin Israel Notarte, Nathaniel Goldrich, David Kavteladze, Brandon Michael Henry, Ferdinando Mannello
Summary: SARS-CoV-2 infection can cause a wide range of symptoms, and platelet indices have attracted great interest due to their involvement in COVID-19-related thromboinflammation. A systematic review found significantly elevated platelet distribution width (PDW) values in COVID-19 infected cohorts compared to controls, with higher PDW values observed in severe cases. These results suggest that platelets play a critical role in the immunothrombosis process of COVID-19, and PDW can serve as an indicator of platelet heterogeneity.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Critical Care Medicine
Daniela Ligi, Bruna Lo Sasso, Rosaria Vincenza Giglio, Rosanna Maniscalco, Chiara DellaFranca, Luisa Agnello, Marcello Ciaccio, Ferdinando Mannello
Summary: This study found that histones can significantly increase the monocyte distribution width (MDW) index in whole blood samples from healthy individuals when treated in vitro, similar to the changes found in patients with classic and viral sepsis. These changes are associated with histone-induced modifications of monocyte volume, cytoplasmic granularity, vacuolization, and nuclear structure. Therefore, histones may play a crucial role in the observed alterations of monocytes in acute classical and viral sepsis.
Review
Medicine, General & Internal
Rosaria Vincenza Giglio, Bruna Lo Sasso, Luisa Agnello, Giulia Bivona, Rosanna Maniscalco, Daniela Ligi, Ferdinando Mannello, Marcello Ciaccio
JOURNAL OF CLINICAL MEDICINE
(2020)
