期刊
ARCHIVES OF VIROLOGY
卷 158, 期 5, 页码 1055-1064出版社
SPRINGER WIEN
DOI: 10.1007/s00705-012-1585-3
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资金
- State Basic Research Program of China [2010CB126203, 2012CB722504]
- International Science & Technology Cooperation Program of China [2012DFA30900]
- National Natural Science Foundation of China [31071660]
- Program for Zhejiang Leading Team of Science and Technology Innovation
- Program for Leading Team of Agricultural Research and Innovation of Ministry of Agriculture, China
Rice black-streaked dwarf virus (RBSDV), a plant-infecting reovirus (genus Fijivirus), generally induces virus-containing tubules in infected cells. The nonstructural protein P7-1, encoded by the first open reading frame of segment 7, is involved in forming the structural matrix of these tubules. In experiments to investigate the subcellular localization of P7-1 in Nicotiana benthamiana epidermal cells, fluorescence of P7-1:eGFP was observed in the nucleus, cytoplasm and cell periphery, and in punctate points along the cell wall of plasmolyzed cells. Co-localization with plasmodesmata-located protein 1 showed that P7-1 formed the punctate points at plasmodesmata. Mutational analysis demonstrated that transmembrane domain 1 and adjacent residues were necessary and sufficient for P7-1 to form punctate structures at the cell wall in the plasmolyzed cells. Chemical drug and protein inhibitor treatments indicated that P7-1 utilized the ER-to-Golgi secretory pathway and the actomyosin motility system for its intracellular transport. The plasmodesmatal localization of RBSDV P7-1 is therefore dependent on the secretory pathway and the actomyosin motility system.
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