期刊
ARCHIVES OF VIROLOGY
卷 157, 期 5, 页码 975-979出版社
SPRINGER WIEN
DOI: 10.1007/s00705-012-1240-z
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资金
- Japanese Ministry of Health, Labor and Welfare [21-Shinko-Ippan-005]
- Japan Society for the Promotion of Science [22590387]
- Grants-in-Aid for Scientific Research [21406012, 22590387] Funding Source: KAKEN
Culex flavivirus (CxFV) is an insect-specific flavivirus that has recently been detected in various Culex spp. mosquitoes worldwide. Here, we report the successful construction of a full-length infectious cDNA clone of a Tokyo strain, CxFV-NIID21. The full-length CxFV-NIID21 cDNA was cloned into the low-copy-number plasmid pMW119, which was stably amplified in Escherichia coli. Transfection of a mosquito cell line with in vitro-transcribed RNA from the cDNA clone resulted in the production of recombinant progeny virus with growth properties, cytopathogenicity, and virion morphology similar to the parental virus.
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