Article
Microbiology
Payuda Hansoongnern, Nantawan Phecharat, Ketkaew Wasanasuk, Wantanee Tommeurd, Penpitcha Chankeeree, Chalermpol Lekcharoensuk, Ploypailin Semkum, Sunan Pinitkiatisakul, Porntippa Lekcharoensuk
Summary: In this study, a recombinant plasmid carrying CpG ODN was designed and constructed to incorporate CpG ODN into porcine circovirus type 2 (PCV2) virus-like particles (VLPs) for enhancing the immunogenicity of the vaccine. The CpG ODN-loaded VLPs significantly increased PCV2-specific neutralizing antibodies and interferon gamma (IFN-gamma) expression in immunized mice compared to VLPs alone.
VETERINARY MICROBIOLOGY
(2022)
Review
Veterinary Sciences
Libin Wen, Kongwang He
Summary: PCV2, associated with PCVAD in pigs, has caused economic losses in the pig industry worldwide. Since its discovery in the late 1990s, PCV2 has evolved with new genotypes and recombination between different genotypes.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Veterinary Sciences
Yanwei Li, Pingping Yu, Yaxuan Bao, Yuwen Ren, Shaowei Zhao, Xuexian Zhang
Summary: This study aimed to establish an indirect enzyme-linked immunosorbent assay (ELISA) based on virus-like particles (VLPs) to detect antibodies against porcine circovirus 2 (PCV-2) in pigs. The PCV-2 VLPs showed good immunogenicity and stimulated high levels of PCV-2 antibodies. The developed ELISA exhibited diagnostic sensitivity and specificity of 98.33% and 93.33% compared to immunofluorescence assay (IFA), respectively, and had good repeatability.
BMC VETERINARY RESEARCH
(2023)
Article
Immunology
Kiju Kim, Tae-Wook Hahn
Summary: The study evaluated the protective immune responses of an experimental PCV2d-based subunit vaccine in pigs and found that the vaccine significantly increased PCV2b- or PCV2d-specific IgG and neutralizing antibody levels without interference by maternal antibodies. Additionally, vaccination with the PCV2d-based subunit vaccine led to a significant increase in serum IL-4 levels and a reduction in PCV2d viremia levels compared to the control group.
Article
Virology
Yangkun Liu, Xueying Han, Yaqi Qiao, Tiejun Wang, Lunguang Yao
Summary: This study generated porcine deltacoronavirus (PDCoV) virus-like particles (VLPs) for the first time using a baculovirus expression vector system. Electron microscopy analysis showed that PDCoV VLPs appeared as spherical particles with a diameter similar to native virions. Furthermore, PDCoV VLPs effectively induced PDCoV-specific immune responses in mice, including the production of IgG and neutralizing antibodies as well as the stimulation of cytokine production.
Article
Spectroscopy
Izabel C. V. P. Gogone, Glaucia H. Ferreira, Danielle Gava, Rejane Schaefer, Fabiola F. de Paula-Lopes, Raquel de A. Rocha, Flavia R. O. de Barros
Summary: The study evaluated Raman spectroscopy as a diagnostic tool for porcine parvovirus and porcine circovirus type 2, demonstrating its ability to effectively discriminate virus-infected cells and providing promising bases for future diagnostic applications.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Immunology
Wen Zhang, Zhendong Fu, Hongyan Yin, Qingbing Han, Wenhui Fan, Fangkun Wang, Yingli Shang
Summary: PCV2 infection skews macrophages towards M1 status by reprogramming the expression of M1 and M2 associated genes. This skewing is regulated by activation of NF-κB and JNK signaling pathways for M1-associated genes and suppression of M2-associated genes through inhibition of JMJD3 expression. This modulation by PCV2 contributes to bacterial coinfection and viral pathogenesis.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Genetics & Heredity
Rajib Deb, Joyshikh Sonowal, Gyanendra Singh Sengar, Seema Rani Pegu, Manas Ranjan Praharaj, Waseem Akram Malla, Indra Singh, Ajay Kumar Yadav, Swaraj Rajkhowa, Pranab Jyoti Das, Jaya Bharati, Souvik Paul, Vivek Kumar Gupta
Summary: The goal of this study was to compare the global gene expression profile in pig cardiac tissues infected with porcine circovirus 2 (PCV2) and healthy cells. The study identified 196 significantly differentially expressed genes (DEGs) and revealed their involvement in biological pathways related to cardiac pathology.
Article
Immunology
Luis Giovani Oliveira Guardalini, Paulo Eduardo da Silva Cavalcante, Jaci Leme, Renata Gois de Mello, Thaissa Consoni Bernardino, Simone Goncalves Silva Jared, Marta Maria Antoniazzi, Renato Mancini Astray, Aldo Tonso, Eutimio Gustavo Fernandez Nunez, Soraia Attie Calil Jorge
Summary: This study aimed to evaluate the scalability from Schott flasks to a stirred-tank bioreactor for production of rabies-recombinant baculovirus and rabies virus-like particles (VLP) using Sf9 insect cells as a host. Equivalent assays were performed in both systems and multivariate statistical approach was used to maximize VLP production. The hydrodynamic and chemical differences in the two systems greatly affected the virus and VLP integrity.
Article
Immunology
Lanjeong Ju, Usharani Jayaramaiah, Min-A Lee, Young-Ju Jeong, Su-Hwa You, Hyang-Sim Lee, Bang-Hun Hyun, Nakhyung Lee, Seok-Jin Kang
Summary: This study evaluated the efficacy of a VLP vaccine against PCV2d in a farm environment. The results showed that vaccinated piglets had lower viremia levels and higher body weight gain compared to the control group after natural infection.
Article
Microbiology
Haochun Pan, Changchao Huan, Yutong Hou, Ping Yan, Fan Yang, Luyao Jiang, Song Gao
Summary: The infection of porcine circovirus type 2 (PCV2) triggers the activation of the PERK pathway and leads to DNA damage. This study reveals that IGFBP3 may regulate DNA damage via ER stress to mediate PCV2 replication. The overexpression of IGFBP3 enhances PCV2 replication by activating the PERK/eIF2 alpha pathway-mediated induction of DNA damage. Targeting this pathway could be a potential antiviral strategy.
VETERINARY MICROBIOLOGY
(2023)
Review
Virology
Jinshuo Guo, Lei Hou, Jianwei Zhou, Dedong Wang, Yongqiu Cui, Xufei Feng, Jue Liu
Summary: Porcine circovirus type 2 (PCV2) infection causes significant economic losses to the global swine industry. Vaccination, both commercially available and experimental, has shown efficacy in preventing and controlling PCV2-associated disease. Adjuvants can enhance the protective immunity of vaccines. Further research and development of superior vaccines are needed for effective prevention and control of PCV2 infection.
Article
Microbiology
Haochun Pan, Changchao Huan, Wei Zhang, Yutong Hou, Ziyan Zhou, Jingting Yao, Song Gao
Summary: Porcine circovirus type 2 (PCV2) infection enhances the expression of porcine PDZ Domain-Containing 1 (PDZK1) and overexpression of PDZK1 inhibits PCV2 replication. PDZK1 regulates PCV2 replication by controlling nitric oxide production via the PI3K/ERK2 signaling pathway. PDZK1 also modulates IL-4 expression through the PI3K/ERK2 pathway.
VETERINARY MICROBIOLOGY
(2022)
Article
Immunology
Yu-an Li, Yanni Sun, Yuqin Zhang, Shifeng Wang, Huoying Shi
Summary: This study demonstrates the potential of using Salmonella Choleraesuis vectors to deliver VLPs and provides new ideas for the development of PCV2 vaccines.
Article
Immunology
Yu -an Li, Yanni Sun, Yuqin Zhang, Shifeng Wang, Huoying Shi
Summary: This study demonstrates the potential of using S. Choleraesuis vectors to deliver virus-like particles (VLPs) of porcine circovirus type 2 (PCV2), which can induce specific immune responses and neutralizing antibodies against the virus.
