期刊
ARCHIVES OF TOXICOLOGY
卷 83, 期 10, 页码 925-932出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00204-009-0453-8
关键词
Conus litteratus; M-superfamily conotoxin; Tetrodotoxin-sensitive sodium channels; Rat dorsal root ganglion neurons; Post-translational modification
类别
资金
- Ministry of Science and Technology of China [2006AA090504, 2008AA09Z401]
- National Natural Science Foundation of China [30871921]
- Guangdong Science-Tech Program [2006A36501001]
- Guangdong Natural Science Foundation [8151027501000083]
In this work, a novel M-superfamily conotoxin, designated lt3a, was purified from the crude venom of Conus litteratus. Combined with peptide sequencing, MALDI-TOF mass spectrometry and cDNA cloning techniques, the amino acid sequence of lt3a was supposed to be D gamma CC gamma OQWCDGACDCCS, where O is hydroxyproline and gamma is carboxyglutamate. The Cys framework of lt3a (-CC-C-C-CC-) is similar to that of psi-, mu-, kappa M-conotoxins, which are representatives of M-conotoxins. Peptide lt3a is categorized into M1 branch based on the number of residues in the last Cys loop. Whole cell patch-clamp study on adult rat dorsal root ganglion neurons indicated that lt3a could enhance tetrodotoxin-sensitive sodium currents. This is a previously unknown function of M-superfamily conotoxins.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据