Article
Agricultural Engineering
Juana Rozalen, M. Mercedes Garcia, Amaya Zalacain, Horacio Lopez-Corcoles, Jorge Hurtado de Mendoza, Katrina Cornish, Manuel Carmona
Summary: Guayule, as a promising renewable source of natural rubber/latex, has resins with various activities potentially related to sesquiterpenes. By exploring different analytical methods, it was found that there may be new members of the guayulins family and the relationship in seasonal changes was identified.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Chemistry, Analytical
Bingbing Liu, Xuanlin Meng, Kun Li, Jinggong Guo, Zongwei Cai
Summary: This study used MALDI-MSI to investigate the spatial distribution of lipids in cottonseeds, revealing heterogeneous distribution patterns of different lipid species within the seeds. The roles of these lipids in metabolic pathways were analyzed, and the relationship between their spatial distribution and functions was studied. The unique distribution patterns of lipid metabolites revealed by MSI may offer new insights into the spatial compartmentation of lipid metabolism in plants and can potentially aid genetic engineering efforts when combined with transcriptomics and proteomics.
Review
Chemistry, Analytical
Hugo M. Santos, Luis B. Carvalho, Carlos Lodeiro, Goncalo Martins, Ines L. Gomes, Wilson D. T. Antunes, Vanessa Correia, Maria M. Almeida-Santos, Helena Rebelo-de-Andrade, Antonio P. A. Matos, J. L. Capelo
Summary: This work presents a tutorial on the capabilities of bioanalytical mass spectrometry in detecting and differentiating viruses at the peptide level, as well as providing diagnosis and prognosis for infected patients. It offers insights into the possibilities of current high-resolution mass spectrometry technology and bioinformatics.
MICROCHEMICAL JOURNAL
(2023)
Article
Food Science & Technology
Jing Wan, Peng He, Yuanshan Chen, Qiujin Zhu
Summary: A practical, efficient, and rapid method for quantifying 19 pyrethroids in tea and orange samples was developed. Out of 262 tea samples, 11 pyrethroids were detected positive with concentrations below maximum residue limits. No pyrethroid was found in 209 peeled orange samples.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Alan B. Moran, Richard A. Gardner, Manfred Wuhrer, Guinevere S. M. Lageveen-Kammeijer, Daniel I. R. Spencer
Summary: Sialic acids play diverse biological roles and linkage-specific analysis is important in glycomic studies. The combination of procainamide fluorescent labeling with sialic acid linkage-specific derivatization is presented, which allows for unambiguous assignment of sialic acid linkages and separation of sialylated isomers.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yousef S. Elshamy, Timothy G. Strein, Lisa A. Holland, Chong Li, Anthony DeBastiani, Stephen J. Valentine, Peng Li, John A. Lucas, Tyler A. Shaffer
Summary: This study introduces a new method of nanoflow sheath CE-VSSI-MS, which reduces the reliance on separation flow rate to facilitate the transfer of analyte to the MS. By using the nanoflow sheath, higher separation voltages and more flexible separation conditions can be achieved.
ANALYTICAL CHEMISTRY
(2022)
Article
Agriculture, Multidisciplinary
Sinah Reiter, Andreas Dunkel, Amira Metwaly, Julian Panes, Azucena Salas, Dirk Haller, Thomas Hofmann
Summary: The study introduces a method for quantitating a wide range of bile acids and applies it to investigate the connection between microbiome alterations and bile acid metabolism in Crohn's disease. The research explores the potential use of bile acid profiles to predict disease state in the complex mechanism of Crohn's disease development.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Construction & Building Technology
Stepanka Kuckova, Gabriela Rambouskova, Petra Junkova, Jiri Santrucek, Pavel Cejnar, Tatiana Anatolievna Smirnova, Ondrej Novotny, Radovan Hynek
Summary: The enrichment of mortar mixtures with natural organic materials has a long history, with protein additives proven to enhance mortar durability and strength. This study aimed to identify and quantify proteins in model mortars with various protein additives using mass spectrometry techniques. The results confirmed the possibility of identifying protein additives in century-old mortars, shedding light on traditional recipes.
CONSTRUCTION AND BUILDING MATERIALS
(2021)
Article
Biochemical Research Methods
Cuijiao Zhan, Changmao Wang, Yaqin Wang, Haitang Xie, Jiru Chu, Rong Zhang, Rongfeng Hu, Jie Shen, Yuanwei Jia
Summary: The study developed and validated a highly efficient method for detecting meloxicam in human plasma, using stable isotope-labeled internal standard. The method was fully validated for specificity, sensitivity, accuracy, and other parameters, providing a reliable basis for pharmacokinetic studies of meloxicam in healthy Chinese volunteers. Key pharmacokinetic parameters were determined after a single dose administration of meloxicam.
BIOMEDICAL CHROMATOGRAPHY
(2021)
Review
Spectroscopy
Annunziata Lapolla
Summary: This article introduces the collaboration and research progress between the research group in the Department of Medicine at Padua University and the mass spectrometric group at CNR of Padua in the field of nonenzymatic protein glycation. The development of new mass spectrometric techniques has allowed for in-depth investigation of the effects of browning and glycation levels on proteins, revealing the role of advanced glycation end products in diabetes complications.
MASS SPECTROMETRY REVIEWS
(2023)
Article
Neurosciences
Merve Karayel-Basar, Irep Uras, Irem Kiris, Ahmet Tarik Baykal
Summary: Huntington's disease is a neurodegenerative disease with no cure that impairs daily activities. This study used MALDI-MSI and LC-MS/MS to analyze protein changes in the brain tissue of an HD mouse model. Significant differences in protein levels were found in different stages of the disease, and the changes in Tau, EF2, HSP70, and NogoA proteins were validated.
EUROPEAN JOURNAL OF NEUROSCIENCE
(2023)
Article
Biochemical Research Methods
Yaju Zhao, Qiaobo Liao, Kai Xi, Danke Xu
Summary: A two-dimensional MoS2 nanosheet prepared by chemical exfoliation method was found to be a superior matrix for small molecule detection in MALDI-TOF MS, especially showing better performance in negative ion mode for analysis of various compounds. The ionization mechanism of MoS2 in negative ion mode was discussed, demonstrating better sensitivity and reproducibility. MoS2-assisted LDI MS provides a simple and rapid approach for quantitative determination of sulfamethoxine in serum samples, offering potential for drug therapy monitoring.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Review
Food Science & Technology
Ramona Abbattista, Giovanni Ventura, Cosima Damiana Calvano, Tommaso R. Cataldi, Ilario Losito
Summary: The increase in olive oil consumption has led to a significant amount of by-products such as olive leaves, pomace, and olive oil mill wastewaters. These by-products contain bioactive compounds that can be valuable for various industries, but their extraction and recovery require detailed identification and quantification. Analytical methods like LC-MS are commonly used to identify and quantify these bioactive compounds, with MS being a useful tool in providing structural information.
Review
Chemistry, Analytical
Negin Fasih Ramandi, Mohammad Faranoush, Alireza Ghassempour, Hassan Y. Aboul-Enein
Summary: The recent success in studying the proteome as a source of biomarkers has revolutionized our understanding of leukemia. The use of mass spectrometry techniques to identify differentially expressed proteins has led to improved diagnosis, prognosis, and treatment strategies for leukemia. This review focuses on the use of various ionization sources and mass analyzers in monitoring different types of leukemia, with MALDI-TOF MS identified as a quick and reliable method for protein analysis. Additionally, the review classifies over 400 proteins and identifies specific biomarkers for acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL), and other stages of leukemia for future studies.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
William Craig Byrdwell, Hari K. Kotapati, Robert Goldschmidt, Pavel Jakubec, Lucie Novakova
Summary: The study demonstrates the use of split-flow two-dimensional liquid chromatography (SF-2D-LC) and quadruple parallel mass spectrometry (LC3MS4) for analyzing NIST SRM 1849a adult/infant formula, achieving successful three-dimensional chromatographic separation with optimized separation space and mass spectrometry methods.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Pharmacology & Pharmacy
Jung Gi Lee, Jae Ha Ryu, Seon-Myung Kim, Moon-Young Park, San-Ho Kim, Young G. Shin, Jong-Woo Sohn, Ha Hyung Kim, Zee-Yong Park, Jae Young Seong, Jae Il Kim
BIOCHEMICAL PHARMACOLOGY
(2018)
Article
Biochemical Research Methods
Jin-Ju Byeon, Min-Ho Park, Seok-Ho Shin, Byeong ill Lee, Yuri Park, Jangmi Choi, Nahye Kim, Yeonjae Kang, Young G. Shin
BIOMEDICAL CHROMATOGRAPHY
(2018)
Article
Biochemical Research Methods
Min-Ho Park, Jin-Ju Byeon, Seok-Ho Shin, Nahye Kim, Yuri Park, Byeong Ill Lee, Jangmi Choi, Young G. Shin
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2018)
Article
Pharmacology & Pharmacy
Yuri Park, Nahye Kim, Jangmi Choi, Min-Ho Park, Byeong ill Lee, Seok-Ho Shin, Jin-Ju Byeon, Young G. Shin
Article
Biochemistry & Molecular Biology
Byeong ill Lee, Min-Ho Park, Seok-Ho Shin, Jin-Ju Byeon, Yuri Park, Nahye Kim, Jangmi Choi, Young G. Shin
Article
Biochemistry & Molecular Biology
Jin-Ju Byeon, Min-Ho Park, Seok-Ho Shin, Yuri Park, Byeong Ill Lee, Jang-mi Choi, Nahye Kim, Seo-jin Park, Min-jae Park, Jeong-hyeon Lim, Young-Guk Na, Young G. Shin
Article
Biochemistry & Molecular Biology
Byeong Ill Lee, Min-Ho Park, Jin-Ju Byeon, Seok-Ho Shin, Jangmi Choi, Yuri Park, Yun-Hee Park, Jeiwook Chae, Young G. Shin
Article
Biochemistry & Molecular Biology
Yuri Park, Min-Ho Park, Jin-Ju Byeon, Seok-Ho Shin, Byeong Ill Lee, Jang-mi Choi, Nahye Kim, Seo-jin Park, Min-jae Park, Jeong-hyeon Lim, Young G. Shin
Article
Biology
Seok-Ho Shin, Yuri Park, Min-Ho Park, Jin-Ju Byeon, Byeong Ill Lee, Jangmi Choi, Young G. Shin
Article
Pharmacology & Pharmacy
Byeong ill Lee, Seo-jin Park, Yuri Park, Seok-Ho Shin, Jang-mi Choi, Min-jae Park, Jeong-hyeon Lim, Sun Young Kim, Hyangsook Lee, Young G. Shin
Summary: The OHPAS linker exhibited stability in both in vitro and in vivo experiments, while the VC-PABC linker showed relative instability. This is attributed to the sensitivity of the VC-PABC linker to a hydrolytic enzyme present in mouse plasma. Further experiments are needed to fully evaluate the efficacy and toxicity of the OHPAS linker.
Article
Biochemistry & Molecular Biology
Jangmi Choi, Min-Ho Park, Seok-Ho Shin, Jin-Ju Byeon, Byeong Ill Lee, Yuri Park, Young G. Shin
Summary: The study found that the concentration of SAS in mouse brain was much lower than in plasma, with only a small percentage of SAS metabolized into significant metabolites SP and Ac-SP. The results suggest that metabolites such as SP and Ac-SP may be responsible for the pharmacological effects in the brain, rather than SAS itself.
Article
Chemistry, Multidisciplinary
Seo-jin Park, Byeong ill Lee, Min-Ho Park, Jangmi Choi, Yuri Park, Min-jae Park, Jeong-hyeon Lim, Jiyu Lee, Sangsoo Hwang, Jeongmin Lee, Young G. Shin
Summary: Trastuzumab emtansine (T-DM1) is the first FDA-approved ADC for metastatic HER2+ breast cancer. An immuno-affinity capture LC-MS/MS assay was developed to quantify the antibody-conjugated drug of T-DM1, which was successfully applied to evaluate linker stability in vitro and preclinical PK study in rats. This assay could be beneficial for other ADCs with similar linker-cytotoxic drug platform.
APPLIED SCIENCES-BASEL
(2021)
Article
Biochemistry & Molecular Biology
Minjae Park, Byeong Ill Lee, Jangmi Choi, Yuri Park, Seo-Jin Park, Jeong-Hyeon Lim, Jiyu Lee, Young G. Shin
Summary: A LC-qTOF-MS assay was developed for the evaluation of drug metabolism and pharmacokinetics properties of Daporinad in mice. The method showed good linearity, accuracy, precision, and stability. In vitro and in vivo metabolite identification studies were conducted, revealing a total of 25 metabolites and ten different types of metabolism.
Article
Biochemistry & Molecular Biology
Jeong-Hyeon Lim, Minjae Park, Yuri Park, Seo-Jin Park, Jiyu Lee, Sangsoo Hwang, Jeongmin Lee, Yujin Lee, Eunjeong Jo, Young G. Shin
Summary: There have been attempts to improve the pharmacokinetic characteristics of anti-tumor small-molecule drugs by conjugating them with large molecules such as monoclonal antibodies (ADCs). Albumin, a abundant protein in the blood, has also been proposed as a large molecule to be conjugated with anti-cancer small-molecule drugs. However, few studies have been conducted for the in vivo prepared albumin-drug conjugates due to the lack of robust bioanalytical methods. In this study, a bioanalytical method using immunoprecipitation and LC-qTOF/MS was developed for the quantification of albumin-conjugated MAC glucuronide phenol linked SN-38 in mouse plasma.
Article
Biochemical Research Methods
Byeong Ill Lee, Min-Ho Park, Soon Chul Heo, Yuri Park, Seok-Ho Shin, Jin-Ju Byeon, Jae Ho Kim, Young G. Shin
BIOMEDICAL CHROMATOGRAPHY
(2018)