期刊
ARCHIVES OF OTOLARYNGOLOGY-HEAD & NECK SURGERY
卷 136, 期 12, 页码 1260-1266出版社
AMER MEDICAL ASSOC
DOI: 10.1001/archoto.2010.219
关键词
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资金
- NCI NIH HHS [P30 CA046592, P50 CA097248-09, P50 CA097248, P30 CA046592-23] Funding Source: Medline
- NIDCD NIH HHS [T32 DC005356-04, P30 DC005188-09, 5 T32 DC005356, P30 DC005188, T32 DC005356] Funding Source: Medline
- NIDCR NIH HHS [R01 DE019126-04, R01 DE019126] Funding Source: Medline
Objective: To design in vitro and in vivo models of metastasis to study the behavior of cancer stem cells (CSCs) in head and neck squamous cell carcinoma (HNSCC). Design: Cells were sorted for CD44 expression using flow cytometry. Sorted cells were used in an in vitro invasion assay. For in vivo studies, CSCs and non-CSCs were injected into the tail veins of mice, and lungs were either harvested or imaged to evaluate for lesions. Results: In vitro, CD44(high) cells were more motile but not more invasive than CD44(low) cells. In vivo, 8 of 17 mice injected with CD44(high) cells and 0 of 17 mice injected with CD44(low) cells developed lung lesions. Two of the lesions arose from CSCs from a primary tumor and 6 from CSCs from HNSCC cell lines. Conclusions: In vitro, CSCs do not have an increased ability to invade through basement membrane, but they migrate more efficiently through a porous barrier. In contrast, CSCs efficiently formed lung lesions in vivo, whereas non-CSCs did not give rise to any distant disease. This phenomenon could be due to the enhanced migratory capacity of CSCs, which may be more important than basement membrane degradation in vivo. Arch Otolaryngol Head Neck Surg. 2010;136(12):1260-1266
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