Article
Food Science & Technology
Margherita D'Alessandro, Flavia Pisanu, David Baldo, Carola Parolin, Gianfranco Filippini, Beatrice Vitali, Rosalba Lanciotti, Francesca Patrignani
Summary: The study found that all microbial mixtures exhibited high viability for 90 days of storage, and there was a more significant decrease in cell viability for non-encapsulated microbial combinations compared to the encapsulated strains in the in vitro digestion results.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Multidisciplinary Sciences
Maximilien Clabaut, Amine M. Boukerb, Amine Ben Mlouka, Amandine Suet, Ali Tahrioui, Julien Verdon, Magalie Barreau, Olivier Maillot, Agathe Le Tirant, Madina Karsybayeva, Coralie Kremser, Gerard Redziniak, Cecile Duclairoir-Poc, Chantal Pichon, Julie Hardouin, Pascal Cosette, Sylvie Chevalier, Marc G. J. Feuilloley
Summary: The study found that 17 beta -estradiol affects the membrane dynamics and adhesion phenotype of Lactobacillus crispatus strains, especially promoting aggregation potential in the vaginal strain V4. However, there was no significant effect on membrane dynamics in V4 compared to strain CIP104459.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Fengyuan Qi, Shangrong Fan, Chao Fang, Lan Ge, Jinli Lyu, Zhuoqi Huang, Shaowei Zhao, Yuanqiang Zou, Liting Huang, Xinyang Liu, Yiheng Liang, Yongke Zhang, Yiyi Zhong, Haifeng Zhang, Liang Xiao, Xiaowei Zhang
Summary: This study evaluated the efficacy of oral Chinese-origin Lactobacillus with adjuvant metronidazole in treating Chinese BV patients. The results showed that oral administration of probiotics did not improve cure rates, but it effectively restored vaginal health after cure and mainly acted through the intestine.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
J. J. Ahire, S. Sahoo, M. S. Kashikar, A Heerekar, S. G. Lakshmi, R. S. Madempudi
Summary: In this study, lactobacillus strains isolated from healthy reproductive age Indian women were screened for beneficial probiotic properties. The strains showed survival capabilities under various conditions, inhibited the growth of pathogens, and could adhere and co-aggregate with them. They also secreted exopolysaccharides and formed biofilms. Safety assessment indicated their potential for human trials to prevent/treat vaginal dysbiosis.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Food Science & Technology
Jia-Juan Wu, Qin-Yu Zhou, Dong-Mei Liu, Jie Xiong, Ming-Hua Liang, Jun Tang, Yi-Qian Xu
Summary: This study reported the whole genome sequence of Lactobacillus gasseri LGZ1029 and speculated its safety and probiotic characteristics based on gene analysis, indicating its potential for antimicrobial activity.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Microbiology
Chiara Argentini, Federico Fontana, Giulia Alessandri, Gabriele Andrea Lugli, Leonardo Mancabelli, Maria Cristina Ossiprandi, Douwe van Sinderen, Marco Ventura, Christian Milani, Francesca Turroni
Summary: This study investigates the colonization mechanism and antimicrobial activity of Lactobacillus crispatus in the vaginal ecological niche. The results reveal the genetic variability of this species and its adaptation to the niche to enhance competitiveness for colonization.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biotechnology & Applied Microbiology
Jie Zhang, Lili Li, Tong Zhang, Jin Zhong
Summary: In this study, a new type of enzyme called GlgU was identified in L. crispatus, which showed optimal activity at acidic pH and exhibited substrate specificity towards glycogen. This finding contributes to the understanding of glycogen metabolism in the dominant species of human vaginal microbiota.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Wenqian Huang, Yirong Zhang, Zhi Li, Minjie Li, Fangfang Li, Monika Mortimer, Liang-Hong Guo
Summary: Medical applications of nanotechnology show promise, but the effects of nanodrugs on human microbiota have not been fully considered. This study found that medically relevant nanoparticles can affect the metabolism of beneficial bacteria and potentially alter the microbiota and immune signaling.
Article
Microbiology
Annelot C. Breedveld, Heleen J. Schuster, Robin van Houdt, Rebecca C. Painter, Reina E. Mebius, Charlotte van der Veer, Sylvia M. Bruisten, Paul H. M. Savelkoul, Marjolein van Egmond
Summary: This study found that the vaginal microbiota dominated by Lactobacillus crispatus is associated with higher levels of bacterial IgA coating compared to other microbiota compositions. Similar to its role in the intestinal tract, IgA may be involved in maintaining the L. crispatus-dominated microbiota in the female genital tract, which may contribute to the health benefits associated with L. crispatus.
Article
Biotechnology & Applied Microbiology
Leonardo Mancabelli, Walter Mancino, Gabriele Andrea Lugli, Christian Milani, Alice Viappiani, Rosaria Anzalone, Giulia Longhi, Douwe van Sinderen, Marco Ventura, Francesca Turroni
Summary: The vaginal microbiota is predominantly composed of Lactobacillus genus, with Lactobacillus crispatus strains associated with a healthy vaginal environment. A study of 94 healthy vaginal samples revealed that L. crispatus was the most representative species and negatively correlated with pathogenic bacteria. The genetic adaptation of L. crispatus strains to their ecological niche was shown through comparative genomics analysis, and in vitro experiments demonstrated the species' ability to modulate the vaginal microbial consortia.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
MaryClaire Chamberlain, Sarah O'Flaherty, Natalia Cobian, Rodolphe Barrangou
Summary: Lactobacillus species are important residents of the human gastrointestinal tract that can be influenced by probiotic supplements and dietary changes, ultimately affecting host health. The growth of four Lactobacillus strains in the presence of pomegranate extract was studied, revealing the production of unique metabolites. Untargeted metabolomics was utilized to further characterize the metabolic profiles of these strains. Understanding these differences is crucial for understanding probiotic function and selecting strains for commercial products.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Engineering, Chemical
YanJun Qu, ChangHao Yu, TianJun Gan, BaoQi Huang, ShuYuan Huang, Jing Yu, Ping Song
Summary: Appropriate cryoprotectants are crucial for effective probiotic preparations. In this study, Lactobacillus gasseri H87 was combined with crop by-products to determine a suitable cryoprotectant for use in vaginal capsules. 15% skim milk (SM) was found to ensure high survival rate and maximum bacteriocin production, and the addition of 1% pomegranate peel extract (PPE) further enhanced the bacteriostatic effect. Lyophilized L. gasseri H87 vaginal capsules preserved with 15% SM + 1% PPE showed inhibitory activity against Lactobacillus iners after 6 months of storage. These findings suggest that skim milk combined with PPE can serve as a suitable cryoprotectant for the production of L. gasseri H87 vaginal capsules in the pharmaceutical industry.
Article
Multidisciplinary Sciences
Maximilien Clabaut, Amandine Suet, Pierre-Jean Racine, Ali Tahrioui, Julien Verdon, Magalie Barreau, Olivier Maillot, Agathe Le Tirant, Madina Karsybayeva, Coralie Kremser, Gerard Redziniak, Cecile Duclairoir-Poc, Chantal Pichon, Sylvie Chevalier, Marc G. J. Feuilloley
Summary: Research shows that 17 beta-estradiol has direct effects on Lactobacillus crispatus, affecting membrane dynamics and promoting biosurfactant production and adhesion to vaginal cells. These effects are important for the physiology of the vaginal environment, contributing to lactobacillus adhesion to mucosa and protection against pathogens.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Dae Hee Han, Woon-ki Kim, Cheonghoon Lee, SungJun Park, Kiuk Lee, Sung Jae Jang, GwangPyo Ko
Summary: This study investigated the effects of co-administration of L. gasseri KBL697 and IFX on colitis in mice. The results showed that the combined treatment improved inflammation symptoms, modulated inflammatory cytokine production, and affected gut microbiota and metabolic pathways.
SCIENTIFIC REPORTS
(2022)
Article
Nutrition & Dietetics
Xianping Li, Yuchun Xiao, Yuanming Huang, Liqiong Song, Mengde Li, Zhihong Ren
Summary: This study demonstrates that the use of Lactobacillus gasseri RW2014 probiotics can lower blood cholesterol levels and improve fatty liver. The study also found that LGA can reduce levels of inflammatory factors, increase levels of short-chain fatty acids, and alter the composition of gut microbiota. Additionally, LGA regulates bile acid metabolism. These findings suggest that LGA may lower cholesterol by modulating bile acid metabolism and gut microbiota composition.
Article
Biotechnology & Applied Microbiology
Mar Margalef-Catala, Elena Stefanelli, Isabel Araque, Karoline Wagner, Giovanna E. Felis, Albert Bordons, Sandra Torriani, Cristina Reguant
Article
Food Science & Technology
N. Martinez-Onandi, A. Castioni, E. San Martin, A. Rivas-Canedo, M. Nunez, S. Torriani, A. Picon
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2017)
Article
Biotechnology & Applied Microbiology
E. Bargossi, G. Tabanelli, C. Montanari, V. Gatto, F. Chinnici, F. Gardini, S. Torriani
JOURNAL OF APPLIED MICROBIOLOGY
(2017)
Article
Food Science & Technology
Renato L. Binati, Giada Innocente, Veronica Gatto, Alessandro Celebrin, Maurizio Polo, Giovanna E. Felis, Sandra Torriani
FOOD RESEARCH INTERNATIONAL
(2019)
Article
Food Science & Technology
Renato L. Binati, Wilson J. F. Lemos Junior, Giovanni Luzzini, Davide Slaghenaufi, Maurizio Ugliano, Sandra Torriani
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2020)
Article
Agriculture, Dairy & Animal Science
Lorenzo Siroli, Francesca Patrignani, Margherita D'Alessandro, Elisa Salvetti, Sandra Torriani, Rosalba Lanciotti
Article
Microbiology
Veronica Gatto, Renato L. Binati, Wilson J. F. Lemos, Arianna Basile, Laura Treu, Otavio G. G. de Almeida, Giada Innocente, Stefano Campanaro, Sandra Torriani
MICROBIOLOGICAL RESEARCH
(2020)
Article
Food Science & Technology
R. L. Binati, W. J. F. Lemos Junior, S. Torriani
Summary: The study showed that inoculating certain non-Saccharomyces yeasts in wine can significantly increase GSH concentration, especially Metschnikowia spp. This strategy can serve as an alternative to pure GSH or inactivated dry yeasts, and help reduce the need for sulfur dioxide in wine production.
AUSTRALIAN JOURNAL OF GRAPE AND WINE RESEARCH
(2021)
Article
Microbiology
Elisa Salvetti, Ilenia Campedelli, Ilaria Larini, Giada Conedera, Sandra Torriani
Summary: In this study, in silico analyses were conducted to explore AR genes in Leuconostoc genus, revealing 131 putative AR traits across 17 type strains and identifying the lsaA gene in certain strains for the first time. The distribution and potential roles of lsaA in different Leuconostoc nontype strains were discussed, along with assessment of MIC towards CLI and QUD, suggesting implications for antibiotic resistance and reassessment of resistance thresholds in certain species.
Article
Microbiology
Andrea Bordugo, Elisa Salvetti, Giulia Rodella, Michele Piazza, Alice Dianin, Angela Amoruso, Giorgio Piacentini, Marco Pane, Sandra Torriani, Nicola Vitulo, Giovanna E. Felis
Summary: In a study on an infant diagnosed with PA through NBS, metronidazole treatment effectively reduced propionate-producing Bacteroides fragilis, while supplementation with bifidobacteria increased their population and improved microbial biodiversity. Metabarcoding analysis proved to be a useful tool for fast and cost-effective monitoring, offering potential for tailored microbiota modification treatment studies to enhance the quality of life for PA patients.
Article
Food Science & Technology
Nicola Ferremi Leali, Renato L. Binati, Francesco Martelli, Veronica Gatto, Giovanni Luzzini, Andrea Salini, Davide Slaghenaufi, Salvatore Fusco, Maurizio Ugliano, Sandra Torriani, Elisa Salvetti
Summary: This study provides insights into the metabolic signatures of strains in kombucha microbial community, facilitating the standardization of kombucha production and demonstrating the roles played by different strains in the fermentation process.
Article
Biotechnology & Applied Microbiology
Eleonora Troiano, Ilaria Larini, Renato L. Binati, Veronica Gatto, Sandra Torriani, Pietro Buzzini, Benedetta Turchetti, Elisa Salvetti, Giovanna E. Felis
Summary: This study identified 85 consensus single-copy orthologs from the whole-genome sequencing data and validated three of them as unsuitable phylogenetic markers. The average nucleotide identity (ANI) calculation confirmed that strain DBT012 belongs to M. pulcherrima.
FEMS YEAST RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Renato L. Binati, Maret Du Toit, Jacky L. Snoep, Elisa Salvetti, Sandra Torriani
Summary: The study showed that Lactiplantibacillus plantarum IWBT B063 exhibited differential expression of 148 functionally annotated genes during growth in synthetic grape juice medium under different oxygen exposure conditions and low availability of nitrogen-based nutrients. These genes were mainly involved in carbohydrate metabolism, genetic information processing, and signaling and cellular processes, highlighting the potential adaptive capacity of this strain under stress conditions.
FERMENTATION-BASEL
(2021)
Article
Biotechnology & Applied Microbiology
Ilaria Checchia, Renato L. Binati, Eleonora Troiano, Maurizio Ugliano, Giovanna E. Felis, Sandra Torriani
Summary: This study evaluated the impact of grape washing, SO2 addition, and selected starter culture inoculation on population dynamics, fermentation kinetics, and main oenological parameters in lab-scale trials. The results showed that grape washing was effective in removing undesirable microorganisms, while inoculation of multi-starter cultures had a greater impact on fermentation rates.
FERMENTATION-BASEL
(2021)
Article
Chemistry, Analytical
Giulio Rosati, Arianna Cunego, Fabio Fracchetti, Antonio Del Casale, Matteo Scaramuzza, Alessandro De Toni, Sandra Torriani, Alessandro Paccagnella