Article
Biochemistry & Molecular Biology
Kun-hsuan Ho, Shey-chiang Su, Kuan-rong Lee
Summary: This study investigated the association between sulbactam and two-component regulatory systems (TCS) in Acinetobacter baumannii using molecular docking and simulation analyses. The results suggest that sulbactam may interfere with bacterial multiplication by interacting with the active sites of BaeSR and AdeSR.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Microbiology
Xiaochen Liu, Yunjie Chang, Qingye Xu, Wang Zhang, Zhen Huang, Linyue Zhang, Shanshan Weng, Sebastian Leptihn, Yan Jiang, Yunsong Yu, Xiaoting Hua
Summary: The widespread prevalence of multi-drug-resistant A. baumannii (MDRAB) is a significant challenge for treatment. Cefiderocol is a promising antibiotic for MDRAB infections. This study demonstrated that mutations in baeS and baeR reduce susceptibility to cefiderocol by increasing the expression of efflux pumps. The study also revealed the regulatory effects of BaeSR on other operons and provided insights into cefiderocol resistance and virulence in A. baumannii.
Article
Microbiology
Matthew M. Schaefers, Benjamin X. Wang, Nicole M. Boisvert, Sarah J. Martini, Sarah L. Bonney, Christopher W. Marshall, Michael T. Laub, Vaughn S. Cooper, Gregory P. Priebe
Summary: Mutations in the fixL gene of BCC play a significant role in bacterial adaptation during chronic lung infection in CF patients, enhancing survival in macrophages and the murine lung environment at the expense of reduced fitness in soil.
Article
Biochemistry & Molecular Biology
Xiamei Kang, Xiao Zhou, Yanting Tang, Zhijie Jiang, Jiaqi Chen, Muhammad Mohsin, Min Yue
Summary: Salmonella enterica, serovar Gallinarum biovar Pullorum is a pathogen specific to avians, causing significant economic losses in the poultry industry globally. The study analyzed the genome of S. Pullorum strain S06004 and identified 22 pairs of two-component systems (TCSs) belonging to five families. The role of the CitB family in S. Pullorum pathogenesis was investigated using mutants generated through the CRISPR/Cas9 system. It was found that the CitB family does not affect bacterial growth, biochemical properties, invasion, proliferation, or fimbrial protein expression. However, the mutants showed enhanced biofilm formation, antimicrobial resistance, inhibition tolerance, and virulence in chicken embryos. Deletion of the Dpi TCS had detrimental effects on survival in hyperosmotic and oxidative environments, as well as long-term colonization of the chicken small intestine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Mingfa Lv, Sixuan Ye, Ming Hu, Yang Xue, Zhibin Liang, Xiaofan Zhou, Lianhui Zhang, Jianuan Zhou
Summary: This study identified a two-component system associated with the regulation of bacterial virulence in the phytopathogen Dickeya oryzae. The system, composed of a histidine kinase ArcB and a response regulator ArcA, modulates cell motility, biofilm formation, and bacterial virulence. It was found that ArcA binds to the promoters of genes involved in cellulose synthesis, biofilm formation, and virulence regulation. However, deletion of the coding genes for ArcB and ArcA did not affect the production of cell wall degrading enzymes and zeamines. Overall, this study unveils the role of a new two-component system in the pathogenesis of Dickeya oryzae.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Bihan Wu, Shuang Zhao, Xuejiao Yang, Laicheng Zhou, Yang Ma, Hongyue Zhang, Wenbin Li, Huaimin Wang
Summary: This work describes the rational design of tetrapeptides to drive the formation of liquid crystalline hydrogel through intermolecular interactions. It also reveals the mechanism of self-assembly and the formation of anisotropic liquid crystalline domains. The diameter of nanofibers can be controlled by varying the enantiomer of electrostatic pairs. Furthermore, the hydrogel properties can be easily controlled by incorporating a light-responsive group.
Article
Mathematics
Wenguang Cheng
Summary: This paper focuses on the Cauchy problem of the two-component Fornberg-Whitham system, which lacks the L2-norm conservation law of the component u. We establish two new wave-breaking results for this system by utilizing the sign preservation of P, the L1-norm conservation law of P, and an a priori estimate for the L2-norm of u.
JOURNAL OF DIFFERENTIAL EQUATIONS
(2023)
Article
Food Science & Technology
Qian Guo, Yu Zhang, Xiaowei Fang, Yuying Yang, Xiongyan Liang, Jing Liu, Chun Fang
Summary: Listeria monocytogenes is a ubiquitous foodborne pathogen that forms persistent biofilms in food processing environments, posing a significant threat to human health and food safety. The two-component system virS/virR positively regulates motility and biofilm formation in L. monocytogenes, and its deletion reduces both traits. This study provides valuable insights for controlling biofilm risks of L. monocytogenes in food safety.
Review
Microbiology
Alexandra A. Guffey, Patrick J. Loll
Summary: VRE poses a serious threat to human health and new therapeutics are urgently needed. The VanRS two-component system, with its key molecules VanS and VanR, may provide a potential target for new treatment options.
Review
Medicine, General & Internal
Junqi Zhang, Rong Fang, Qi Peng, Shizhou Wu, Lei Lei
Summary: Enterococcus faecalis (E. faecalis) is a highly adaptable Gram-positive, facultative anaerobic bacterium that can cause serious infections with biofilm formation. The regulation of biofilm formation in E. faecalis involves polysaccharides, autolysis, proteases, and eDNA, which are regulated by important regulatory systems including the two-component signal transduction system (TCS). The WalRK system, as one of the 17 TCSs and the only essential one in E. faecalis, plays a crucial role in modulating various metabolisms, drug resistance, and interactions with the host immune system.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Multidisciplinary Sciences
Catalin M. Bunduc, Dirk Fahrenkamp, Jiri Wald, Roy Ummels, Wilbert Bitter, Edith N. G. Houben, Thomas C. Marlovits
Summary: The T7SS inner-membrane complex of Mycobacterium tuberculosis is unique in structure and essential for virulence, with the protease MycP(5) playing a crucial role in complex integrity. This study reveals a previously undescribed mechanism of protein transport and provides a potential target for drug development against this major human pathogen.
Article
Engineering, Mechanical
K. Prabith, I. R. Praveen Krishna
Summary: This paper investigates the influence of rub parameters on the stability of a two-spool rotor system undergoing rub-impact. It explores the effects of rotor-stator contact stiffness, coefficient of friction, and clearance on system response and stability, and observes different responses between co-rotation and counter-rotation modes. Increasing friction coefficient leads to early onset of bifurcations, while higher contact stiffness and clearance delay bifurcation onset. The response characteristics of co-rotating and counter-rotating systems are found to be distinct, with counter-rotating systems entering the quasi-periodic regime more rapidly.
NONLINEAR DYNAMICS
(2021)
Article
Microbiology
Jakub M. Kwiecinski, Diamond A. Jelani, Ernesto J. Fuentes, Alexander R. Horswill
Summary: This study describes two novel inhibitors of the two-component regulatory system ArlRS in Staphylococcus aureus and demonstrates their high efficacy and specificity. These inhibitors disrupt the signaling pathway of S. aureus and result in the loss of virulence traits, showing significant therapeutic potential.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Mathematics
Min Guo, Fang Wang, Shengqi Yu
Summary: In this paper, the local well-posedness of a two-component Novikov system 1+1 in critical Besov spaces is established, with discussions on uniform bound, convergence, regularity, uniqueness, and continuity of solution map.
Article
Microbiology
Ju Sun, Siting Wen, Zhichao Wang, Wei Liu, Yan Lin, Jiayun Gu, Weiting Mao, Xiaojuan Xu, Qigai He, Xuwang Cai
Summary: This study investigated the impact of epinephrine (Epi) on capsular polysaccharide synthesis in G. parasuis and the specific mechanism of G. parasuis in response to Epi. The results showed that Epi significantly inhibited CPS expression, affecting biofilm formation and bacterial adhesion. Through molecular experiments, researchers discovered that the QseBC two-component system played a regulatory role in the process.
MICROBIOLOGY SPECTRUM
(2023)
Review
Microbiology
Fernando Gil, Ivan L. Calderon, Juan A. Fuentes, Daniel Paredes-Sabja
FUTURE MICROBIOLOGY
(2018)
Article
Infectious Diseases
R. Alvarez, O. Inostroza, M. Garavaglia, N. P. Minton, D. Paredes-Sabja, F. Gil
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2018)
Article
Microbiology
Juan Munoz-Miralles, Bruno C. Trindade, Pablo Castro-Cordova, Ingrid L. Bergin, Leslie A. Kirk, Fernando Gil, David M. Aronoff, Daniel Paredes-Sabja
FUTURE MICROBIOLOGY
(2018)
Article
Microbiology
Paulina Calderon-Romero, Pablo Castro-Cordova, Rodrigo Reyes-Ramirez, Mauro Milano-Cespedes, Enzo Guerrero-Araya, Marjorie Pizarro-Guajardo, Valeria Olguin-Araneda, Fernando Gil, Daniel Paredes-Sabja
Article
Biochemical Research Methods
Christian Brito-Silva, Jaime Pizarro-Cerda, Fernando Gil, Daniel Paredes-Sabja
JOURNAL OF MICROBIOLOGICAL METHODS
(2018)
Article
Microbiology
Jan Nevermann, Andres Silva, Carolina Otero, Diego P. Oyarzun, Boris Barrera, Fernando Gil, Ivan L. Calderon, Juan A. Fuentes
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Microbiology
Pablo Castro-Cordova, Fernando Diaz-Yanez, Juan Munoz-Miralles, Fernando Gil, Daniel Paredes-Sabja
Article
Biochemistry & Molecular Biology
Lillian G. Acuna, M. Jose Barros, Paula Nunez, Diego Penaloza, Fernanda Montt, Diego Pedraza, Katherine Crossley, Fernando Gil, Juan A. Fuentes, Ivan L. Calderon
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Microbiology
R. Alvarez, C. Ortega-Fuentes, C. Queralto, O. Inostroza, F. Diaz-Yanez, R. Gonzalez, I. L. Calderon, J. A. Fuentes, D. Paredes-Sabja, F. Gil
MICROBIOLOGICAL RESEARCH
(2020)
Article
Microbiology
Lillian G. Acuna, M. Jose Barros, Fernanda Montt, Diego Penaloza, Paula Nunez, Ivan Valdes, Fernando Gil, Juan A. Fuentes, Ivan L. Calderon
Summary: Small noncoding RNAs, such as RyhB, play vital roles in gene expression and physiology regulation in bacteria. In this study, two RyhB homologs, RyhB-1 and RyhB-2, were identified and characterized in the fish pathogen Yersinia ruckeri. Despite similar expression patterns under iron starvation, mutant strains lacking RyhB-1 or RyhB-2 exhibited distinct phenotypes, indicating non-redundant roles in modulating bacterial physiology.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Microbiology
Fernando Diaz-Yanez, Ricardo Alvarez, Ivan L. Calderon, Juan A. Fuentes, Fernando Gil
Article
Microbiology
Pedro Marchant, Alexander Carreno, Eduardo Vivanco, Andres Silva, Jan Nevermann, Carolina Otero, Eyleen Araya, Fernando Gil, Ivan L. Calderon, Juan A. Fuentes
Summary: While some bacteria's outer membrane vesicles (OMVs) provide resistance to various antimicrobial agents, their role in polymyxin resistance in S. Typhi has not been fully explored.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Diego Penaloza, Lillian G. Acuna, M. Jose Barros, Paula Nunez, Fernanda Montt, Fernando Gil, Juan A. Fuentes, Ivan L. Calderon
Summary: The sRNAs RyhB-1 and RyhB-2 from S. Typhimurium play vital roles in bacterial infection by regulating intracellular survival, replication, metabolic status, and gene expression associated with Salmonella pathogenicity. Additionally, they directly interact with key bacterial genes, contributing to virulence modulation inside macrophages.
Article
Microbiology
Ivan L. Calderon, Maria Jose Barros, Fernanda Montt, Fernando Gil, Juan A. Fuentes, Lillian G. Acuna
Summary: The RNA chaperone Hfq plays an important role in antibiotic persistence in Yersinia ruckeri, as demonstrated by faster replication and decreased antibiotic tolerance in the hfq-knockout mutant. Hfq is also dependent on the (p)ppGpp synthetase RelA for persister cells production, with the Delta relA and Delta relA Delta hfq strains showing defects in persister cells formation compared to the wild type. The findings shed light on the participation of Hfq in antibiotic persistence in Y. ruckeri.
Article
Microbiology
Fernando Gil, Sebastian Lagos-Moraga, Paulina Calderon-Romero, Marjorie Pizarro-Guajardo, Daniel Paredes-Sabja