期刊
ARCHIVES OF MICROBIOLOGY
卷 192, 期 8, 页码 663-672出版社
SPRINGER
DOI: 10.1007/s00203-010-0591-7
关键词
Pseudomonas syringae; Psychrophilic bacterium; Aspartate aminotransferase; Transposon mutagenesis; Cold-inducible gene
类别
资金
- Council of Scientific and Industrial Research, New Delhi, Government of India
- CSIR
CSM2, a cold-sensitive mutant of psychrophilic Pseudomonas syringae, grows like wild-type cells when cultured at 22 and 28A degrees C; but at 4A degrees C, the growth is retarded. In CSM2, AAT (coding for aspartate aminotransferase) is identified as the mutated gene. The expression of AAT in Pseudomonas syringae was transiently enhanced when cells were shifted from 22 to 4A degrees C indicating that AAT is cold-inducible. Complementation of the mutated AAT transformed CSM2 from a cold-sensitive phenotype to a cold-resistant phenotype like the wild-type cells, thus providing evidence for the first time that AAT is required for low-temperature growth.
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