Article
Microbiology
Ziqi Lu, Jian Guo, Qiang Li, Yatao Han, Zhen Zhang, Zhongna Hao, Yanli Wang, Guochang Sun, Jiaoyu Wang, Ling Li
Summary: This study investigated the dynamics of peroxisomes in the conidia germination of Alternaria alternata using enhanced green fluorescent protein (eGFP) tagged with peroxisome targeting signal 2 (PTS2). The results showed that the number of peroxisomes decreased gradually during spore germination, but increased in the germ tubes. Additionally, when the transformants were cultured on lipid media, the numbers of peroxisomes increased significantly.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shaina Dhamija, Arijit K. De
Summary: The study investigated the photoconversion process in EGFP through 254 nm irradiation, revealing spreading of an isosbestic point and an initial lag phase in absorbance spectral kinetics, indicating sequential photoconversion through an intermediate. Unique fluorescence lifetimes were assigned to estimate fluorescence kinetics of EGFP and its photoproduct, with time-resolved fluorescence anisotropy studies suggesting minimal structural changes in the protein scaffold upon photoconversion.
PHOTOCHEMISTRY AND PHOTOBIOLOGY
(2021)
Article
Biophysics
Ying Yang, Sanjun Fan, James A. Webb, Yuanqing Ma, Jesse Goyette, Xueqian Chen, Katharina Gaus, Richard D. Tilley, Justin Gooding
Summary: This study presents an electrochemical approach to reversible fluorescence switching of enhanced green fluorescent proteins (EGFP) on indium tin oxide coated glass. The method allows efficient switching between bright (ON) and dim (OFF) states at the single molecule level. The electrochemical fluorescence switching is fast, reversible, and can be incorporated into advanced fluorescence microscopy.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Nanoscience & Nanotechnology
Wubing Chen, Yilong Wang, Yongjie Ying
Summary: This study analyzed the effect of CHFR fusion enhanced green fluorescent protein (EGFP) on nasopharyngeal carcinoma cells, and found that CHFR can significantly inhibit cell proliferation and tumorigenic ability of NPC cells. Up-regulating CHFR expression could also significantly suppress the activity of Aurora A and the expression of CDC2 and CyclinB1.
Article
Biotechnology & Applied Microbiology
Guo-Teng Liang, Cuixin Lai, Zejun Yue, Hanbin Zhang, Danyang Li, Zhong Chen, Xingyu Lu, Liang Tao, Fedor V. Subach, Kiryl D. Piatkevich
Summary: In this study, a pair of engineered flavin-binding fluorescent proteins, miniGFP1 and miniGFP2, were developed. These miniGFPs showed improved photochemical properties and high sensitivity to transition metal ions, making them potential fluorescent biosensors for live cell imaging and metal ion detection.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Plant Sciences
Jinseung Jeong, Byeongho Moon, Inhwan Hwang, Dong Wook Lee
Summary: Protein import into chloroplasts and mitochondria is influenced by the folding states of preproteins, with chloroplast machinery being more tolerant to different folding states, while mitochondrial machinery being specialized in translocating unfolded preproteins.
Article
Chemistry, Analytical
Elena Benito-Pena, Maria C. Moreno-Bondi, Bettina Glahn-Martinez, Giacomo Lucchesi, Fernando Pradanas-Gonzalez, Ana Isabel Manzano, Angeles Canales, Gabriella Caminati
Summary: This study utilized FKBP1A as an alternative to antibodies for biosensing purposes, specifically for monitoring the immunosuppressant drug FK506. The method demonstrated good selectivity and accuracy in determining the drug in blood samples from organ transplant patients.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Fengxia Tian, Guangling Xu, Suo Zhou, Shuchang Chen, Dongmei He
Summary: This review summarizes recent advancements in developing GFP-based biosensors. Researchers have utilized GFP for designing fluorescence biosensors due to their intrinsic fluorescence, stability, and mutability. The construction approaches for GFP-based biosensors are categorized into four types, and their applications in biomolecule detection and life sciences are highlighted. The review also discusses challenges associated with GFP-based biosensors and proposes future research directions.
Article
Fisheries
Xiaoyu Liu, Zhiyang Huang, Yupeng Miao, Pan Li, Yuehong Wang, Zhendong Xu, Xiaodong Zhang, Yongwei Wei
Summary: In this study, the EGFP gene was successfully inserted into the SCRV genome and a recombinant virus expressing EGFP was rescued. The recombinant virus had a high titer and stable expression of EGFP in EPC cells. In vitro and in vivo studies were conducted to investigate the growth characteristics, pathogenicity, and organ tropism of the recombinant virus. This study is of significant importance for understanding the pathogenesis of SCRV and the development of vaccines and antiviral drugs.
Article
Biochemistry & Molecular Biology
Joanna Krasowska, Katarzyna Pierzchala, Agnieszka Bzowska, Laszlo Forro, Andrzej Sienkiewicz, Beata Wielgus-Kutrowska
Summary: This study investigates the ROS scavenging mechanisms of EGFP, demonstrating its high ability to quench various forms of ROS and suggesting a photoprotective role in living systems. Damage to the EGFP chromophore under strong photo-oxidative conditions was also observed. This research contributes to the understanding of the protective function of GFP-like proteins and their possible interactions with ROS in biological systems.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Yucui Hou, Xiaoling Lu, Jie Yang, Chunhua Tang, Hanbing Jiang, Tongji Cai, Meilun Chen, Zheng Wei, Peng Yu
Summary: This study developed an unlabeled fluorescent sensor based on SYBR Green I for the detection of testosterone. The sensor showed high specificity and good performance, allowing for quantitative detection in real water samples, such as tap water and river water. It provides a convenient and efficient alternative method for the quantitative detection of testosterone in the environment.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Multidisciplinary
Ying Guo, Min Yan Zheng, Wei Zhang, Dao Dao Hu
Summary: In this study, water-soluble green fluorescent carbon dots (G-CDs) were prepared using p-phenylenediamine and glutathione (GSH) as precursors. A fluorescent sensing platform using G-CDs and AuNPs was constructed for detection of clenbuterol. The prepared sensor showed excellent sensitivity and linearity, and was successfully applied for CLB detection in pork liver.
Article
Virology
Madhu Rai, Uddhav Timilsina, Ritu Gaur
Summary: This study reports the construction and characterization of a full-length and infectious EGFP-tagged HIV-1 subtype C molecular clone, providing a valuable tool for studying subtype C specific infections. The recombinant virus displays similar viral gene expression, infectivity, and replication kinetics to the parental virus. The presence of the EGFP gene allows for rapid, easy, and quantitative measurement of HIV infection by flow cytometry and fluorescence microscopy.
Article
Chemistry, Multidisciplinary
Mengyuan He, Yu Xiao, Yuanhang Wei, Bo Zheng
Summary: A simple and portable paper-based analytical device based on green emitting carbon dots was developed for visual and semiquantitative detection of ferric ion in real water samples.
Article
Environmental Sciences
Kawsalya Ramasamy, Sarathikannan Dhavamani, Geetha Natesan, Karthik Sengodan, Senthil-Nathan Sengottayan, Manish Tiwari, Sahi Shivendra Vikram, Venkatachalam Perumal
Summary: This study demonstrated the phytofabrication of titanium dioxide nanoparticles wrapped with bioactive molecules, showcasing their applications in photocatalysis and biomedicine. The results highlighted the effective antibacterial and anticancer properties of biomolecule-loaded TiO2NPs.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
