期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 63, 期 28, 页码 6410-6421出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.5b02261
关键词
Helianthus annuus L.; supercritical carbon dioxide; bioactive compounds; sesquiterpene lactones; heliannuol; flavonoids; cytotoxicity and phytoxicity bioassays
资金
- Ministerio de Economia y Competitividad (MINECO) [AGL2013-42238-R]
- Consejeria de Economia Innovacion y Ciencia, Junta de Andalucia [DP12-TEP-725]
The work described herein is a continuation of our initial studies on the supercritical fluid extraction (SFE) with CO2,of bioactive Substances, from Helianthus annuus L. var. Arianna. The selected SE extract showed high activity in the wheat coleoptile bioassay, in Petri dish phytotoxicity bioassays, and in the hydroponic culture of tomato seeds. Chromatographic fractionations of the extracts and a spectroscopic analysis of the isolated compounds showed 52 substances belonging to 10 different chemical classes, which were mainly sesquiterpene lactones, diterpenes, and flavonoids. Heliannuol M (31), helivypolides K and L (36, 37); and helieudesmanolide B (38) are described for the first time in the literature. Metabolites have been tested in the etiolated wheat coleoptile bioassay with good results in,a noteworthy effect on germination. The most active compounds were also tested on tomato seeds, heliannuol A (30) and leptocarpin (45) being the most active, with values similar to those of the commercial herbicide.
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