Article
Nutrition & Dietetics
Yuchen Li, Gilda Aiello, Enrico Mario Alessandro Fassi, Giovanna Boschin, Martina Bartolomei, Carlotta Bollati, Gabriella Roda, Anna Arnoldi, Giovanni Grazioso, Carmen Lammi
Summary: This study focused on the extraction and hydrolysis of proteins from Chlorella pyrenoidosa (C. pyrenoidosa), showing potential hypotensive and hypoglycemic effects. Peptides derived from this process were found to inhibit ACE and DPP-IV activity effectively, with four peptides predicted to block both enzymes. The stability of these peptides during gastrointestinal digestion was also investigated.
Article
Agriculture, Multidisciplinary
Mengdi Chen, Daodong Pan, Tianqiong Zhou, Xinchang Gao, Yali Dang
Summary: The novel umami peptide IPIPATKT exhibited excellent dual DPP-IV and ACE inhibitory activities, displaying significant physiological effects in cellular and in vivo models.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Food Science & Technology
Rosana Chirinos, Thomas Valente de Oliveira, Fanny Guzman, Ana Aguilar-Galvez, Andres Figueroa-Merma, Romina Pedreschi, David Campos
Summary: The present research aimed to evaluate the antioxidant, antihypertensive and hypoglycemic properties of three peptides synthesised from tarwi. In vitro and in silico studies were conducted, including molecular docking evaluation and ADMET properties assessment. The results showed potential antioxidant capacity and inhibition of ACE and DPP-IV enzymes for the peptides, particularly the YSGWLGL peptide. The predicted ADMET properties suggested their suitability for use as nutraceuticals, functional ingredients or supplements.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Panida Aiemratchanee, Kulachatr Panyawechamontri, Phutthaphorn Phaophu, Onrapak Reamtong, Watanalai Panbangred
Summary: New bioactive peptides with antihypertensive properties were identified from porcine blood, showing potential in the treatment of high blood pressure.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Medicinal
Martin Alain Mune Mune, Yoshikatsu Miyabe, Takeshi Shimizu, Wataru Matsui, Yuya Kumagai, Hideki Kishimura
Summary: In this study, we investigated the bioactive peptides produced from the red alga Gracilariopsis chorda through thermolysin hydrolysis of a water-soluble protein (WSP). The results showed that the WSP hydrolysate exhibited strong ACE inhibitory activity, indicating the potential of G. chorda as a source of ACE-inhibitory peptides. Two novel peptides (IDHY and LVVER) with promising inhibitory effects were identified, and their interaction with human ACE was evaluated through molecular docking. In silico analysis further revealed that the ACE inhibitory peptides were mainly located in the highly solvent accessible alpha-helix.
Article
Food Science & Technology
Hina Kamal, Priti Mudgil, Bincy Bhaskar, Ajayi Feyisola Fisayo, Chee-Yuen Gan, Sajid Maqsood
Summary: The study found that amaranth protein hydrolysates (APHs) have stronger inhibitory effects on ACE, DPP-IV, and AG, demonstrating a potential source for antidiabetic and anti-hypertensive peptides.
JOURNAL OF CEREAL SCIENCE
(2021)
Review
Food Science & Technology
Tolulope Joshua Ashaolu, Thanh-Do Le, Itthanan Suttikhana
Summary: Bioactive peptides derived from food proteins have therapeutic potential against degenerative and cardiovascular diseases, but there is limited information on their stability and bioactivity in food matrices. The effects of food processing and storage on the bioactivity of these peptides are also not well understood.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Food Science & Technology
Adriana CunhaNeves, Padraigin A. Harnedy-Rothwell, Richard J. FitzGerald
Summary: Large quantities of mussel byssus, a fibrous material rich in collagen, are generated as a byproduct of the mussel-processing industry. Extracting collagen from this material using food-friendly strategies has been challenging due to its complex structure. In this study, an enzyme-aided method was employed to extract collagen from mussel byssus, resulting in a yield of 138.82 +/- 2.25 mg collagen/g dry weight. The enzymatic hydrolysates of the extracted collagen showed significant hydrolysis and exhibited high in vitro bioactivities. These findings suggest that mussel byssus collagen-derived hydrolysates have potential applications as functional food ingredients for the management of metabolic diseases.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Waqas N. Baba, Bincy Baby, Priti Mudgil, Chee-Yuen Gan, Ranjit Vijayan, Sajid Maqsood
Summary: This study investigated the ACE inhibiting potential of peptic camel whey hydrolysates under different hydrolysis conditions. Through peptide sequencing and in silico analysis, specific peptides with potential ACE and renin inhibitory activities were identified. Enzymatic hydrolysis of camel whey proteins may release peptides with potential anti-hypertensive properties.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Aleksandr G. Kruchinin, Ekaterina I. Bolshakova, Irina A. Barkovskaya
Summary: This research generated a digital model of the peptide complex of different whey types with predicted bioactivity, safety, and sensory properties using bioinformatic modeling approaches. The study found that hydrolysis by the protease complex chymotrypsin C-subtilisin was the most effective method to release peptides with both antioxidant and ACE-inhibitory activity. It was also observed that the bioactive peptides obtained as a result of in silico hydrolysis after GI digestion simulation can be considered safe in terms of allergic reactions and toxicological effects.
FERMENTATION-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Daraksha Iram, Manish Singh Sansi, Sameena Zanab, Shilpa Vij, Ashutosh, Sunita Meena
Summary: An in silico study was conducted to investigate the hydrolysis of sheep milk proteins and the generation of bioactive peptides with ACE and DPP-IV inhibitory activity. The results showed that sheep milk proteins have the potential to be a source of antidiabetic and hypotensive peptides.
JOURNAL OF FOOD BIOCHEMISTRY
(2022)
Article
Agriculture, Multidisciplinary
Aiyuan Zhang, Kai Wang, Xiaofei Liu, Xuewu Zhang
Summary: This study identified and characterized bioactive peptides derived from the by-product Sacha inchi meal, which showed potent DPP-IV inhibition activity. The inhibition types were mixed, non-competitive, and competitive. The binding sites between peptides and DPP-IV were determined through hydrogen bonding, hydrophobic interaction, and van der Waals force. The peptides could have potential applications in the health food industry and as lead compounds for diabetes therapy.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Food Science & Technology
Juan Wang, Yujia Xie, Yuanyuan Luan, Tingting Guo, Shanshan Xiao, Xingxing Zeng, Shaohui Zhang
Summary: The objective of this study was to isolate and identify intracellular bioactive peptides from mouse lymphocytes before and after lipopolysaccharide (LPS) stimulation. A total of 131 novel peptides were discovered, and their bioactivity and function were predicted. Two peptides with better inhibition activity were verified.
FOOD SCIENCE AND HUMAN WELLNESS
(2022)
Review
Biochemistry & Molecular Biology
Bababode Adesegun Kehinde, Ishrat Majid, Shafat Hussain
Summary: Activating protease enzymes in vegetable foods through the sprouting process can effectively extract bioactive peptides and other nutrient components, with the advantages of easy operation and reduced processing costs. Vegetable sprouts are rich in nutrients and have therapeutic functionalities, and they provide more efficient nutritional benefits compared to their unsprouted counterparts. Sprouting is a convenient approach to improve the health benefits of vegetable foods.
JOURNAL OF FOOD BIOCHEMISTRY
(2022)
Article
Food Science & Technology
Papungkorn Sangsawad, Sasikan Katemala, Danou Pao, Saranya Suwanangul, Rachasit Jeencham, Manote Sutheerawattananonda
Summary: This study aimed to produce bioactive peptides from sericin using enzymatic hydrolysis methods, with neutrase producing peptides with the strongest DPP-IV and ACE inhibitory activities. The peptides remained stable even after simulated in vitro gastrointestinal digestion and ultrafiltration, showing promise as dietary ingredients for alleviating metabolic syndromes.
Article
Biotechnology & Applied Microbiology
Isaac Lisondro, Cintia Gomez Serrano, Claudia Sepulveda, Ariadna Indira Batista Ceballos, Francisco Gabriel Acien Fernandez
Summary: The study investigated the influence of irradiance and operation modes on the biomass production and biochemical composition of the diatom Nitzschia aff. pellucida, particularly focusing on fatty acid profile. Results showed that the growth rate increased with irradiance, but photo-inhibition was observed at higher irradiances. The model proposed considers the modification of saturation constant as a function of available irradiance, and the strain was found to be better adapted to low irradiances.
JOURNAL OF APPLIED PHYCOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Martina Ciardi, Cintia Gomez-Serrano, Maria del Mar Morales-Amaral, Gabriel Acien, Tomas Lafarga, Jose Maria Fernandez-Sevilla
Summary: The study demonstrates that pig slurry can be used as an effective nutrient source for biomass production of Scenedesmus almeriensis, with a biomass productivity rate comparable to standard growth medium. Different dilutions of pig slurry did not significantly affect microalgal growth, while ozonation was effective in reducing the chemical oxygen demand concentration of the media.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Engineering, Chemical
Vitor Verdelho Vieira, Jean-Paul Cadoret, F. Gabriel Acien, John Benemann
Summary: Microalgae, as the basis for an emerging worldwide industry, are facing significant challenges in contributing to the global economy, including adapting traditional industry experiences and regulations to the microalgae sector. This work presents and discusses the main issues and boundaries impacting the microalgae sector, highlighting the need for appropriate regulations and support from governments and businesses to recognize the unique attributes and potential of microalgae.
Article
Biology
Ainoa Morillas-Espana, Angela Ruiz-Nieto, Tomas Lafarga, Gabriel Acien, Zouhayr Arbib, Cynthia Gonzalez-Lopez
Summary: The world population is expected to grow by over 2 billion people, leading to an increased demand for agricultural production. This study focuses on producing high-quality microalgal biomass using wastewater and assessing its biostimulant capacity. The results show the potential of using microalgae as a sustainable source of biostimulants.
Article
Agricultural Engineering
Elisa Clagnan, Giuliana D'Imporzano, Marta Dell'Orto, Ana Sanchez-Zurano, Francisco Gabriel Acien-Fernandez, Biancamaria Pietrangeli, Fabrizio Adani
Summary: Microalgae cultivation is effective in reducing pathogens and purifying wastewater, but a comprehensive understanding of the risks is still needed. Different methods were used to explore microbial community and pathogen presence, showing that media and sampling locations have an impact on community structure and pathogenic loads. The main detected pathogens were Arcobacter and Elizabethkingia (mainly in wastewater), with a significant presence of Aeromonas in all samples. Raceways show potential as an effective biotreatment, with most retained pathogens being released in the outlet and only a small part settling in the biomass.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Elisa Clagnan, Giuliana D'Imporzano, Marta Dell'Orto, Alessia Bani, Alex J. Dumbrell, Katia Parati, Francisco Gabriel Acien-Fernandez, Agustin Portillo-Hahnefeld, Antera Martel-Quintana, Juan Luis Gomez-Pinchetti, Fabrizio Adani
Summary: This study investigated the effects of centrate and synthetic media on microalgal communities, and found that microalgal communities grown on centrate showed higher nitrogen content and richer gene composition.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Rebecca Nordio, Francisco Javier Delgado, Ana Sanchez-Zurano, Jose Gonzalez Hernandez, Enrique Rodriguez-Miranda, Jose Luis Guzman, Tomas Lafarga, Gabriel Acien
Summary: The present study aimed to evaluate the use of microalga Scenedesmus almeriensis for treating untreated urban wastewater. Two 12 m(3) raceway reactors, one supplemented with wastewater and the other with chemical fertilizer, were operated outdoors in a semi-continuous mode for eight months. The results showed that S. almeriensis could be produced in wastewater without affecting the photosynthetic apparatus, with an average productivity of 13 g center dot m(-2)center dot day(-1) in both systems. Moreover, the nutrient content of the wastewater, including nitrogen, phosphorous, and chemical oxygen demand, was reduced to meet European limitations, with average removal rates of 2.2, 0.2, and 3.0 g center dot m(-2)center dot day(-1) respectively. The raceway reactors demonstrated high potential for microalgal production and efficient biotreatment, proving to be robust and reliable. Additionally, an accurate model was developed to evaluate the effect of environmental conditions on biomass productivity in the clean system (R-2 = 0.9, p = 0.0002).
BIORESOURCE TECHNOLOGY
(2023)
Article
Environmental Sciences
Joseph Santhi Pechsiri, Jean-Baptiste E. Thomas, Naoufel El Bahraoui, Francisco Gabriel Acien Fernandez, Jamal Chaouki, Saad Chidami, Rodrigo Rivera Tinoco, Jose Pena Martin, Cintia Gomez, Michel Combe, Fredrik Grondahl
Summary: This study compares the environmental performance of conventional reactors and a proposed internally illuminated novel closed reactor design. The results show that the novel photobioreactor can significantly reduce impacts such as eutrophication and climate change when leveraging renewable energy sources and the photosynthesis process in urban-industrial symbiosis scenarios.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biotechnology & Applied Microbiology
Elvira Navarro-Lopez, Juan Jose Gallardo-Rodriguez, Maria del Carmen Ceron-Garcia, Inmaculada Gallego-Lopez, Francisco Gabriel Acien-Fernandez, Emilio Molina-Grima
Summary: The effect of different parameters on the extraction of biostimulant molecules from microalgal biomass was investigated, and the optimal conditions for using organic green solvents (acetone or ethanol) were determined for maximum germination index.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Article
Food Science & Technology
Silvia Villaro, Gabriel Acien, Cynthia Victoria Gonzalez-Lopez, Elisa Clagnan, Tomas Lafarga
Summary: The present study aimed to produce the microalga Arthrospira platensis BEA 005B using 80 m2 raceway reactors and evaluate its potential use as a food color in macarons. The productivity of the system ranged between 8 and 10 g m- 2.day- 1 depending on the culture depth. The produced biomass mainly consisted of protein and contained valuable pigments.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Jorge Garcia-Marquez, Jesus Vizcaino Antonio, Andre Barany, Alba Galafat, Gabriel Acien, Felix L. Figueroa, Javier Alarcon Francisco, Miguel Mancera Juan, Juan Antonio Martos-Sitcha, Salvador Arijo, Roberto Teofilo Abdala-Diaz
Summary: The study found that the combined administration of Chlorella fusca and ethanol-inactivated Vibrio proteolyticus significantly improved the growth performance and feed utilization of the thick-lipped grey mullet, while enhancing the lipid quality and n-3 polyunsaturated fatty acids. This has potential benefits for human nutrition.
Article
Microbiology
Jorge Garcia-Marquez, Rosa Maria Rico, Francisco Gabriel Acien, Juan Miguel Mancera, Felix L. Figueroa, Antonio Jesus Vizcaino, Francisco Javier Alarcon, Miguel Angel Morinigo, Roberto Teofilo Abdala-Diaz
Summary: This study investigates the effects of a blend of microalgae on Sparus aurata juveniles. The results show that the addition of microalgae tends to decrease body lipids and affects fatty acid profile. In particular, the diet containing 25% of the microalgae blend increases DHA levels. Additionally, this diet promotes appropriate microbial diversity and influences fatty acid composition and lipid metabolism in fish.
Article
Mathematics
Pablo Otalora, Jose Luis Guzman, Manuel Berenguel, Francisco Gabriel Acien
Summary: The industrial production of microalgae is a sustainable and interesting process, especially in terms of its applications in wastewater treatment. Neural network models have been developed to optimize the process and characterize the pH dynamics in different raceway reactors. These models are able to predict pH profiles using available measurable process data and demonstrate the potential of artificial neural networks in modeling continuous dynamic systems in the industry.
Article
Chemistry, Medicinal
Jorge Garcia-Marquez, Bruna Rodrigues Moreira, Piedad Valverde-Guillen, Sofia Latorre-Redoli, Candela T. Caneda-Santiago, Gabriel Acien, Eduardo Martinez-Manzanares, Manuel Mari-Beffa, Roberto T. Abdala-Diaz
Summary: This study investigated the cytotoxic activity of ulvan polysaccharides obtained from Ulva rigida against human cancer cell lines and zebrafish embryos. The results showed that ulvan had cytotoxic effects on human cancer cell lines, particularly on colon cancer cells. However, ulvan also had negative effects on the growth rate and osmolar balance of zebrafish embryos, indicating that its use should be limited to specific concentrations.
Article
Agriculture, Dairy & Animal Science
Jorge Garcia-Marquez, Daniel Alvarez-Torres, Isabel M. Cerezo, Marta Dominguez-Maqueda, Felix L. Figueroa, Francisco Javier Alarcon, Gabriel Acien, Eduardo Martinez-Manzanares, Roberto T. Abdala-Diaz, Julia Bejar, Salvador Arijo
Summary: This study assessed the impact of dietary green microalgae and ethanol-inactivated Vibrio proteolyticus on thick-lipped grey mullet juvenile fish. The results showed that the diet influenced the composition of intestinal microbiota without affecting its functionality. Additionally, the diet effectively regulated the transcription of stress and immune-related genes, enhancing the fish's resistance against stress and infections.
