Article
Multidisciplinary Sciences
Charlotte Kromer, Karin Schwibbert, Ashish K. Gadicherla, Dorothea Thiele, Nithiya Nirmalananthan-Budau, Peter Laux, Ute Resch-Genger, Andreas Luch, Harald R. Tschiche
Summary: Biofilms are widespread in nature and pose potential risks to human health. A fluorescent nanosensor has been developed for monitoring pH levels in biofilms, providing a new tool for studying biofilms.
SCIENTIFIC REPORTS
(2022)
Article
Biochemical Research Methods
Emiliano Martinez-Perinan, Alvaro Martinez-Sobrino, Iria Bravo, Tania Garcia-Mendiola, Eva Mateo-Marti, Felix Pariente, Encarnacion Lorenzo
Summary: In this study, carbon nanodots modified with Neutral Red (NR-CDs) were synthesized using a simple method based on microwave irradiation. The synthesized NR-CDs were characterized and shown to have covalent bonding between Neutral Red molecules and the carbon dot nanostructure. The fluorescence activity of the NR-CDs was explored and applied to develop a new fluorescence DNA hybridization assay for detecting a specific DNA sequence of Escherichia coli bacteria.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Xuetao Hu, Yanxiao Li, Yiwei Xu, Ziyu Gan, Xiaobo Zou, Jiyong Shi, Xiaowei Huang, Zhihua Li, Yahui Li
Summary: Carbon quantum dots synthesized from orange peel were used as a green and efficient fluorescent probe for detecting E. coli, showing high sensitivity and reliability. The fluorescent method based on CQDs-MNPs allows for accurate analysis of contaminated milk samples, demonstrating great potential in ensuring food quality and safety.
Article
Chemistry, Analytical
Qian Duan, Yifan He, Wenqiang Bi, Tao Liang, Zhihong Liu, Zhen Li
Summary: In this study, a NIR-excitable ratiometric nanoprobe based on upconversion nanoparticles and an organic molecule was developed for the reversible detection of hydrogen polysulfide. The nanoprobe showed high selectivity and fast response, enabling the monitoring of the formation and consumption of endogenous hydrogen polysulfide in living cells.
ANALYTICAL CHEMISTRY
(2022)
Article
Electrochemistry
Sallahuddin Panhwar, Adnan Aftab, Hareef Ahmed Keerio, Hasan Ilhan, Mohammad Sarmadivaleh, Ugur Tamer
Summary: Climate change is causing the evolution of novel microbes in the environment, while nanoscience is rapidly advancing to provide environmentally friendly engineering solutions for detecting these microbes in blood and water.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2021)
Article
Chemistry, Multidisciplinary
Monica Paesa, Fernando Almazan, Cristina Yus, Victor Sebastian, Manuel Arruebo, Luis M. Gandia, Santiago Reinoso, Ismael Pellejero, Gracia Mendoza
Summary: This study investigates the antimicrobial performance of polyoxometalate-stabilized gold nanoparticles against Escherichia coli and Staphylococcus aureus, finding that one particular composite exhibits excellent bactericidal efficiency without harming mammalian cells.
Article
Chemistry, Multidisciplinary
Hanyu Qian, Eric Mclamore, Nikolay Bliznyuk
Summary: Reuse of alternative water sources for irrigation is a sustainable approach to reduce water gaps and increase food production, but it poses a risk of bacterial contamination. This study developed a statistical machine-learning framework to accurately predict the concentration of pathogenic organisms, such as E. coli, in untreated surface water using an impedimetric electrochemical aptasensor. The framework improved prediction accuracy and can be used for rapid decision support in hydroponic irrigation.
Review
Biochemical Research Methods
Yoonbin Kim, Luyao Ma, Kang Huang, Nitin Nitin
Summary: This review focuses on the application of bio-based technologies for enhancing the decontamination of raw foods, preventing cross-contamination, and rapidly detecting microbial risks during postharvest processing. The bio-based antimicrobial compositions include delivery systems and coatings developed using cell-based carriers, microbubbles, lipid-based colloidal particles, and biopolymers. The bio-based sensing approaches utilize stable and cost-effective bio-receptors such as antimicrobial peptides, bacteriophages, DNAzymes, and engineered liposomes. These approaches can effectively reduce microbial contamination risks and improve in-situ microbial detection.
CURRENT OPINION IN BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Yinyin Chen, Xingying She, Wei Wu, Xianxiang Wang
Summary: A dual-mode optical probe for the detection of organophosphorus pesticides triazophos (TRP) was developed based on the internal filter effect (IFE) between upconversion nanoparticles (UCNPs) and gold nanoparticles (AuNPs) in the presence of protamine (PRO) and trypsin (TRY). The probe demonstrated high sensitivity for TRP detection with good linear relationship and low detection limits. The recovery of TRP in real food samples also showed satisfactory results.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Qian Cui, Yongjie Zhong, Wenkai Shang, Fuming Deng, Buhua Wang, Jiajia Wu, Peng Wang, Liudang Wan, Keling Wang, Lingchen Fang, Rui Dai, Han Zhang, Rodrigo Ledesma-Amaro, Yunuo Zhang, Jiaomei Huang
Summary: Food safety faces significant challenges in preventing foodborne diseases caused by pathogenic pollution, especially in resource-limited areas. Rapid detection techniques of microorganisms play a crucial role in timely bioanalysis and disease treatment strategies. However, it is difficult for these methods to meet the criteria of simplicity, high specificity and sensitivity, and low cost simultaneously. To address this issue, a microbial photoelectric detection system was developed, utilizing a fluorescent probe detection reagent and a photoelectric sensor detection device. This system combines microbial enzyme targets, selective fluorescent substrate metabolism characteristics, and a photoelectric sensor signal transduction mechanism, resulting in a strong signal with a high signal-to-noise ratio.
Article
Chemistry, Multidisciplinary
Sirilak Wangngae, Thitima Pewklang, Kantapat Chansaenpak, Phongsakorn Ganta, Suphanida Worakaensai, Kittipan Siwawannapong, Surayut Kluaiphanngam, Nawarat Nantapong, Rung-Yi Lai, Anyanee Kamkaew
Summary: This study presents a new chalcone-based fluorescent turn-on probe (3c) responsive to nitroreductase (NTR) activity, which can effectively detect NTR activity and identify bacteria. The probe shows high selectivity and low background signal, with fluorescence enhancement achieved through the push-pull effect of the structure.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Delia Tomoiaga, Jaclyn Bubnell, Liam Herndon, Paul Feinstein
Summary: Griffith proposed the concept of DNA transfer between bacteria in 1928. Researchers have demonstrated that E. coli can accept multiple plasmids simultaneously, resulting in widespread aclonality during colony development. Tracking clonally related bacteria allows for mosaic analysis of gene function. Additionally, it was discovered that clonally related bacterium rods self-organize fractally and can remain linked during colony growth, presenting a potential target for controlling microbiota growth.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Haoran Li, Yubo Wang, Yaohua Li, Wei Wang
Summary: The new method successfully quantifies bacterial viability at a single-cell level using optical tweezers, holding potential in antibiotic susceptibility testing, drug screening, and rapid diagnostics.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Haoran Li, Yubo Wang, Yaohua Li, Wei Wang
Summary: This study presents a new label-free bacterial viability assay based on optical tweezers technology, which quantifies bacterial viability by monitoring bacterial nanomotion in the optical traps. The assay is characterized by short time consumption, simple sample preparations, and no cytotoxicity, making it a reliable method for assessing bacterial viability at the single-cell level.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Wei Tang, Ruo Mi, Lun Wang, Hongqi Chen
Summary: The system combines quantitative image analysis with magnetic separation technology, showing low background interference and high separation efficiency, making it suitable for application in biology.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Food Science & Technology
Yi Xu, Md Mehedi Hassan, Arumugam Selva Sharma, Huanhuan Li, Quansheng Chen
Summary: This review summarized the recent advances in nano-optical strategies for bacteria and their metabolites sensing in the field of food safety. Nano-optical sensors, integrated with various technologies, have greatly improved the sensitivity and reduced the detection time and cost. However, challenges remain in the simple fabrication of biosensors and their practical application in complex matrices.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Review
Chemistry, Analytical
Brij Mohan, Gurjaspreet Singh, Armando J. L. Pombeiro, Alexander A. Solovev, Pawan Kumar Sharma, Quansheng Chen
Summary: As the excessive use of pesticides, drugs, and chemicals in agriculture has raised milk safety concerns, the development of monitoring methods for toxicants in milk samples is of great importance. Metal-organic frameworks (MOFs) materials have shown promise in this area due to their tunable porosity and active functional sites. This review focuses on the electrochemical and luminescence MOF sensors for monitoring contaminants in milk samples, discussing their development, sensitivity, selectivity, and application, as well as the challenges and future directions in next-generation milk monitoring using MOF sensors.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Xiaodan Ding, Waqas Ahmad, Jizhong Wu, Yawen Rong, Qin Ouyang, Quansheng Chen
Summary: An ultrasensitive detection strategy for ciprofloxacin (CIP) has been developed using copper ions-induced charge transfer in poly acrylic acid (PAA) functionalized upconversion nanoparticles (UCNPs)/2,2-bipyridine (bipy) system. Copper ions adhere to the surface of PAA-UCNPs, deactivating fluorescence, while bipy controls aggregation. CIP coordinates with the bipy-Cu complex, causing the release of trapped fluorescence. The method shows a good calibration plot (0.05-1000 ng/mL) and a detection limit of 0.13 ng/mL.
Article
Food Science & Technology
Qin Ouyang, Mingming Zhang, Yongcun Yang, Zia-ud Din, Quansheng Chen
Summary: In this study, a novel fluorescence biosensor was designed for the rapid detection of Staphylococcus aureus in meat. By using mesoporous silica-modified upconversion nanoparticles, the biosensor achieved low detection limits and wide detection range. The results showed that the biosensor had comparable performance to the traditional plate counting method in detecting S. aureus in meat.
Article
Food Science & Technology
Hui Jiang, Jihong Deng, Quansheng Chen
Summary: This study presents a quantitative method utilizing olfactory sensor technique for determining fatty acids in stored wheat. An olfactory sensor array was prepared using chemical dyes, and a dung beetle optimizer algorithm was employed to select the best color variable combination for the sensor. The support vector regression model based on the selected color variables showed the best performance in detecting wheat fatty acid values.
Article
Biochemistry & Molecular Biology
Yuying Wang, Sisi Chen, Wanmei Chen, Jingjing Wang, Kun Li, Chengyi Hong, Kailong Zhang, Quansheng Chen
Summary: Beta-lactoglobulin is a protein found in milk that can cause severe allergic reactions. This study presents a novel fluorescent aptamer biosensor for detecting beta-lactoglobulin, which has a linear detection range of 1-100 ng mL (-1) and a limit of detection of 0.344 ng mL (-1). The method has been successfully used for detecting beta-lactoglobulin in milk samples.
Article
Food Science & Technology
Wenhui Geng, Suleiman A. A. Haruna, Huanhuan Li, Hafizu Ibrahim Kademi, Quansheng Chen
Summary: In this study, a novel colorimetric sensor array (CSA) combined with linear and nonlinear regression models was used to evaluate the freshness of chicken meat based on the occurrence of total volatile basic nitrogen (TVB-N). The TVB-N concentration was determined by steam distillation, and the CSA was fabricated using nine chemically responsive dyes. The emissions of volatile organic compounds (VOCs) were found to be correlated to the utilized dyes. Different regression algorithms were applied and compared, and the nonlinear model based on competitive adaptive reweighted sampling coupled with support vector machines (CARS-SVM) achieved the best results.
Review
Multidisciplinary Sciences
Devaraj Sabarinathan, Arumugam Selva Sharma, Marimuthu Murugavelu, Balakrishnan Kirubasankar, Indhiradevi Balusamy, Zhang Han, Huanhuan Li, Quansheng Chen
Summary: The development of nanoclusters based on incorporating biomolecules shows promising future as stable and high fluorescence biosensors with high sensitivity, detection level, and selectivity. The review provides an overview of recent advancements in synthesizing metal nanoclusters and their applications in food safety analysis.
Article
Chemistry, Applied
Jizhong Wu, Muhammad Zareef, Quansheng Chen, Qin Ouyang
Summary: Consumer preference for matcha is influenced by its physicochemical properties. The combination of visible-near infrared (Vis-NIR) spectroscopy and multivariate analysis allows for rapid and non-invasive evaluation of matcha particle size and the ratio of tea polyphenols to free amino acids (P/F ratio). The study compared different variable selection algorithms and proposed the hybridization of interval combination population analysis (ICPA) and competitive adaptive reweighted sampling (CARS) for selecting characteristic wavelengths to build partial least squares (PLS) models. The results showed that the ICPA-CARS-PLS models performed well in evaluating matcha particle size (Rp = 0.9376) and P/F ratio (Rp = 0.9283), demonstrating the significance of rapid, effective, and nondestructive online monitoring using Vis-NIR reflectance spectroscopy and chemometric models in matcha production.
Article
Chemistry, Applied
Afang Zhu, Shujat Ali, Tianhui Jiao, Zhen Wang, Yi Xu, Qin Ouyang, Quansheng Chen
Summary: A highly structured fluorescent-SERS dual-probe nanocomposite was synthesized for the determination of sulfur-containing gases in water and beer samples. The nanocomposite was prepared by growing an Ag shell on an Au core, modifying with a surfactant and Zn2+, and assembling with MOF-5-NH2 through coordination. The system offered a precise detection of H2S/SO2 in food/environmental settings, with a ultra-low detection limit of 2.26 nM.
Article
Chemistry, Applied
Songguang Zhao, Selorm Yao-Say Solomon Adade, Zhen Wang, Jizhong Wu, Tianhui Jiao, Huanhuan Li, Quansheng Chen
Summary: In this study, near-infrared (NIR) spectroscopy combined with partial least squares (PLS), backpropagation neural network (BPANN), and their combination (PLS-BPANN) were used to monitor the total sugar content of kombucha. A total of 16 mathematical models were constructed and evaluated. The results showed that the PLS-BPANN model outperformed others, with a determination coefficient (R2p) of 0.9437 and a root mean square error of prediction (RMSEP) of 0.8600 g/L, indicating good verification results. The findings suggest that NIR coupled with PLS-BPANN can be a nondestructive and online technique for monitoring total sugar changes.
Article
Chemistry, Analytical
Mingxing Zhao, Hui Jiang, Quansheng Chen
Summary: A new method based on olfactory visualization technology is proposed for qualitative identification of pesticide residues in edible oil. Suitable chemical dyes were selected to prepare sensor arrays, which were used to gather volatile odor information in rapeseed oil samples. The olfactory information was then translated into a visual representation and subjected to principal component analysis for dimensionality reduction. Three different models, SVM, RF, and KELM, were constructed based on the sensor image features for analysis.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Afang Zhu, Shujat Ali, Zhen Wang, Tianhui Jiao, Qin Ouyang, Quansheng Chen
Summary: A highly structured SERS biosensor was developed for the detection of Staphylococcus aureus. The biosensor utilized a chemically modified PDMS film as the SERS substrate, with self-assembled Au@Ag NPs for enhancing detection sensitivity. The bacterial surface was covered with Au@Ag NPs, resulting in more hot spots for improved detection. The biosensor achieved an ultra-low detection response of 16 cfu/mL and showed satisfactory results in the detection of S. aureus in tap water, milk, and fish samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Wencui Kang, Hao Lin, Waqas Ahmad, Huanhuan Li, Quansheng Chen
Summary: A nano-composite colorimetric sensor array coupled with near infrared spectroscopy was developed for the determination of active constituents. The sensor array, composed of chemically responsive dyes doped metal-organic frameworks, showed sensitive interaction with different volatile markers. Chemometric methods were used to separate kombucha broth samples with different fermentation times, enabling the estimation of the chemical and physical properties of tea fermentation broth.
MICROCHEMICAL JOURNAL
(2023)
Review
Chemistry, Analytical
Huanhuan Li, Suleiman A. Haruna, Wei Sheng, Qiyi Bei, Waqas Ahmad, Muhammad Zareef, Quansheng Chen, Zhen Ding
Summary: This review evaluates the progress of SERS-based sensors in detecting and monitoring chemical pollutants in food. Significant advancements have been made in the last decade, and factors leading to improved SERS performance are discussed. The review also summarizes the theory and application of label-free SERS-based sensors.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)