Article
Gastroenterology & Hepatology
Chiaki Matsuzaki, Yukari Nakashima, Ikuto Endo, Yusuke Tomabechi, Yasuki Higashimura, Saki Itonori, Koji Hosomi, Jun Kunisawa, Kenji Yamamoto, Keiko Hisa
Summary: Leuconostoc mesenteroides strain NTM048 produces an exopolysaccharide with adjuvanticity for mucosal vaccination, and the key polymer structure for adjuvanticity was determined to be Gtf2-S-glucan. Gtf2-S-glucan, produced in the supernatant by Gtf2, showed a higher ability to stimulate IgA production and induced higher antigen-specific antibodies in the airway mucosa and plasma compared to native NTM048 EPS. These findings suggest a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS.
Article
Chemistry, Applied
Giulia Bisson, Clara Comuzzi, Elisa Giordani, Denise Poletti, Marta Boaro, Marilena Marino
Summary: This study characterized the chemical and biological properties of an exopolysaccharide EPS_B3 produced by Leuconostoc mesenteroides strain isolated from an Italian cheese. The EPS_B3 displayed antimicrobial, antibiofilm, bifidogenic, antioxidant, and DNA-protective activities. It showed potential applications in functional foods, especially for its antimicrobial activity and ability to improve the viability of bifidobacteria in probiotic foods. Further research is needed to assess its ability to reach the gastrointestinal tract intact.
CARBOHYDRATE POLYMERS
(2023)
Article
Microbiology
Junrui Wu, Danli Yan, Yumeng Liu, Xue Luo, Yang Li, Chengxu Cao, Mo Li, Qi Han, Cong Wang, Rina Wu, Lanwei Zhang
Summary: A novel EPS was extracted from Leuconostoc mesenteroides Shen Nong's (SN)-8, showing potential antioxidant and anticancer effects through structural characterization and experimental results.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Qing Zhang, Jie Wang, Qing Sun, Shu-Ming Zhang, Xiang-Yang Sun, Chan-Yuan Li, Miao-Xin Zheng, Wen-Liang Xiang, Jie Tang
Summary: Strain LM187 and its rEPS414 demonstrate good tolerance and significant antioxidant activity, indicating promising prospects for industrial applications.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Medicinal
Shinsuke Inuki, Hitomi Tabuchi, Chiaki Matsuzaki, Yasunori Yonejima, Keiko Hisa, Ikuo Kimura, Kenji Yamamoto, Hiroaki Ohno
Summary: Exopolysaccharides (EPSs) are widely present in natural products made by bacteria, fungi, and algae, and some of them have interesting biological properties such as anticancer and immunomodulatory activities. Our recent study found that EPSs derived from Leuconostoc mesenteroides ssp. mesenteroides strain NTM048 enhanced the production of mucosal immunoglobulin A (IgA) in mice. In this study, we described the synthesis and evaluation of tetrasaccharide fragments of NTM048 EPS to gain insights into the molecular mechanism responsible for the induction of IgA.
CHEMICAL & PHARMACEUTICAL BULLETIN
(2022)
Article
Biotechnology & Applied Microbiology
Seul-Gi Jeong, In Seong Choi, Ho Myeong Kim, Ji Yoon Chang, Hae Woong Park
Summary: Cold adaptation under supercooling conditions can improve the viability and storage stability of lactic acid bacteria during freeze-drying, with exopolysaccharides playing a protective role. Cold adaptation is a good strategy for long-term storage of Leuconostoc mesenteroides WiKim32.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Seul-Ah Kim, Dong Hyeon Lee, Byung Hee Ryu, Nam Soo Han
Summary: A newly developed PCR method was utilized to rapidly and accurately monitor the population changes of L. mesenteroides DRC1506 during kimchi fermentation, providing an effective means for quality control in the fermentation process.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jia Liu, Peijun Zhang, Bo Wang, Yuting Lu, Liang Li, Yuehong Li, Shaojun Liu
Summary: This experiment demonstrated that Astragalus polysaccharides (APS) had immunomodulatory effects on crucian carp, enhancing immune-related indicators and antiviral abilities. The results showed that APS could significantly improve the immune response and resistance against viral infection in crucian carp and EPC cells infected with SVCV.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2022)
Article
Oncology
Chaoran Zang, Yan Zhao, Ling Qin, Guihai Liu, Jianping Sun, Kang Li, Yanan Zhao, Shoupeng Sheng, Honghai Zhang, Ning He, Peng Zhao, Qi Wang, Xi Li, Yanchun Peng, Tao Dong, Yonghong Zhang
Summary: The study revealed specific T cell responses against CTAs and TAAs in HCC patients. In early-stage HCC patients, SALL4-specific response was the most prominent, followed by MAGE-A3, NY-ESO-1, MAGE-A1, and SSX2. The protective role of CTA-specific responses was suggested for one-year recurrence-free survival after treatment. As HCC progressed, CTA and SALL4-specific T cell responses decreased while AFP-specific response increased, indicating the potential of immunotherapy at an early stage of HCC.
Article
Engineering, Biomedical
Shupei Tang, Lan Zhou, Haiyang He, Liwei Cui, Zhicheng Ren, Yuhang Tai, Zhunyi Xie, Yi Cao, Dongwei Meng, Qiuli Liu, Yuzhang Wu, Jun Jiang, Xinyuan Zhou
Summary: This study developed a novel cancer vaccine that effectively enhances the immune response against tumor cells in the tumor microenvironment. The vaccine activates immune cells, boosts the production of tumor-specific T cells and pro-inflammatory cytokines, and suppresses tumor growth and metastasis.
Article
Immunology
Anne H. Rowley, David Arrollo, Stanford T. Shulman, Abigail Torres, Amornrat O'Brien, Kristine Wylie, Kwang-Youn A. Kim, Susan C. Baker
Summary: Background: Kawasaki disease (KD) is a febrile illness of young childhood that can result in coronary artery aneurysms and death. COVID-19 mitigation strategies resulted in a marked decrease in KD cases worldwide, supporting a transmissible respiratory agent as the cause. Methods: Amino acid substitution scans were performed to develop modified peptides with improved recognition by KD monoclonal antibodies (MAbs). Results: A modified peptide epitope recognized by 20 MAbs from 11 KD patients was reported, suggesting a convergent VH3-74 plasmablast response to a specific protein antigen in children with KD.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Review
Immunology
Selina J. Keppler, Marie Christine Goess, Julia M. Heinze
Summary: Humoral immunity is mediated by ASC specialized in synthesizing antibodies, with over 80% residing in the gut and most expressing IgA. These antibodies play a crucial role in maintaining intestinal homeostasis and protective immunity, while also being involved in immune responses outside of mucosal tissues.
FRONTIERS IN IMMUNOLOGY
(2021)
Editorial Material
Oncology
Celine M. Laumont, Brad H. Nelson
Summary: The research demonstrates that IgA antibodies produced by tumor-infiltrating B cells and plasma cells can enter tumor cells via transcytosis, inhibiting oncogenic signals and enhancing T cell-mediated cytotoxicity. These findings unveil a promising new avenue for immunotherapy.
Article
Fisheries
Mohamed Omar Abdalla Salem, Yigit Tastan, Soner Bilen, Ertugrul Terzi, Adem Yavuz Sonmez
Summary: The study found that dietary supplementation of white mustard oil has positive effects on the growth performance, immune responses, and antioxidant enzyme activities in rainbow trout.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Immunology
Yesica Longueira, Diego S. S. Ojeda, Rocio B. Antivero Battistelli, Lautaro Sanchez, Santiago Oviedo Rouco, Daniel Albano, Eleonora Guevara, Vanesa Valls, Maria A. Pando, Andrea V. V. Gamarnik
Summary: This study evaluated the levels of SARS-CoV-2-specific antibodies in breast milk of breastfeeding vaccinated women. The results showed that the levels of IgA and IgG in breast milk changed after vaccination with vector-based vaccines. This is important for understanding the protection against SARS-CoV-2 in newborns and unvaccinated children through breastfeeding.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Saki Fujita, Yasunori Baba, Yukari Nakashima, Yasuki Higashimura, Kenji Yamamoto, Chiaki Matsuzaki, Minoru Kawagishi
CANADIAN JOURNAL OF MICROBIOLOGY
(2020)
Article
Multidisciplinary Sciences
Michael Jakob Pichler, Chihaya Yamada, Bashar Shuoker, Camila Alvarez-Silva, Aina Gotoh, Maria Louise Leth, Erwin Schoof, Toshihiko Katoh, Mikiyasu Sakanaka, Takane Katayama, Chunsheng Jin, Niclas G. Karlsson, Manimozhiyan Arumugam, Shinya Fushinobu, Maher Abou Hachem
NATURE COMMUNICATIONS
(2020)
Article
Microbiology
Miriam N. Ojima, Aina Gotoh, Hiromi Takada, Toshitaka Odamaki, Jin-Zhong Xiao, Toshihiko Katoh, Takane Katayama
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Nutrition & Dietetics
Shigeru Murakami, Chihiro Hirazawa, Takuma Ohya, Rina Yoshikawa, Toshiki Mizutani, Ning Ma, Mitsuru Moriyama, Takashi Ito, Chiaki Matsuzaki
Summary: The study demonstrated that Sargassum horneri can effectively suppress obesity, diabetes, and other metabolic disorders induced by a high-fat diet, as well as improve insulin resistance. The inhibitory effect of Sargassum horneri on intestinal fat absorption may be one of the key mechanisms for its beneficial effects.
Article
Biotechnology & Applied Microbiology
Miriam N. Ojima, Yuya Asao, Aruto Nakajima, Toshihiko Katoh, Motomitsu Kitaoka, Aina Gotoh, Junko Hirose, Tadasu Urashima, Satoru Fukiya, Atsushi Yokota, Maher Abou Hachem, Mikiyasu Sakanaka, Takane Katayama
Summary: In this study, the authors examined the HMO consumption phenotypes of two bifidobacterial species and found that the diversity of the FL transporter led to uptake specificities of fucosylated sugars ranging from simple trisaccharides to complex hexasaccharides. These findings explain the adaptation and proliferation of bifidobacteria in the competitive and HMO-rich infant gut environment.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Tai-Ying Chiou, Wataru Suda, Kenshiro Oshima, Masahira Hattori, Chiaki Matsuzaki, Kenji Yamamoto, Tomoya Takahashi
Summary: Strain C06_No.73(T) isolated from a traditional Japanese fermented beverage called koso is a novel species belonging to the genus Lentilactobacillus, capable of producing lactic acid. The isolate shows potential as a lactic acid bacterium that can grow under conditions of lower temperature and near neutral pH.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Nutrition & Dietetics
Shigeru Murakami, Chihiro Hirazawa, Rina Yoshikawa, Toshiki Mizutani, Takuma Ohya, Ning Ma, Takahiko Ikemori, Takashi Ito, Chiaki Matsuzaki
Summary: This study investigated the effects of the edible seaweed Campylaephora hypnaeoides on high-fat diet-induced obesity and metabolic diseases in mice. The results showed that C. hypnaeoides could alleviate obesity, hyperglycemia, hepatic steatosis, and hypercholesterolemia by improving antioxidant capacity and reducing inflammation.
NUTRITION & METABOLISM
(2022)
Article
Biotechnology & Applied Microbiology
Chiaki Matsuzaki, Tsukasa Shiraishi, Tai-Ying Chiou, Yukari Nakashima, Yasuki Higashimura, Shin-ichi Yokota, Kenji Yamamoto, Tomoya Takahashi
Summary: Components of lactic acid bacteria with immunostimulatory effects are being increasingly studied for their therapeutic and prophylactic potential as alternatives to antibiotics, cognitive enhancements, and vaccine adjuvants. This study found that lactic acid bacteria belonging to the genus Apilactobacillus possess significantly higher activity in inducing immunoglobulin A (IgA) compared to other species. The differences in the structure of lipoteichoic acids (LTAs) may be related to this distinction in IgA-inducing activity.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Ecology
Miriam N. Ojima, Lin Jiang, Aleksandr A. Arzamasov, Keisuke Yoshida, Toshitaka Odamaki, Jinzhong Xiao, Aruto Nakajima, Motomitsu Kitaoka, Junko Hirose, Tadasu Urashima, Toshihiko Katoh, Aina Gotoh, Douwe van Sinderen, Dmitry A. Rodionov, Andrei L. Osterman, Mikiyasu Sakanaka, Takane Katayama
Summary: This study applied assembly theory to investigate the formation of bifidobacterial communities in the infant gut. The results showed that arrival order and sugar consumption phenotypes significantly affected community formation. Bifidobacterium bifidum and Bifidobacterium longum subsp. infantis dominated through inhibitory priority effects, while Bifidobacterium breve benefited from facilitative priority effects and utilized a specific degradant to dominate. This study highlights the importance of initial community assembly and its impact on the maturation trajectory of the infant gut microbiota.
Article
Biochemistry & Molecular Biology
Nozomi Ishii, Hiroshi Muto, Mitsuo Nagata, Kanae Sano, Itsuki Sato, Kenta Iino, Yuji Matsuzaki, Toshihiko Katoh, Kenji Yamamoto, Ichiro Matsuo
Summary: In this study, a fluorescence-quenching-based assay system was developed to determine the hydrolytic activity of ENGases. The system was evaluated using various ENGases and showed promising results in assessing the substrate specificities of these enzymes.
CARBOHYDRATE RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Hiromi Takada, Toshihiko Katoh, Mikiyasu Sakanaka, Toshitaka Odamaki, Takane Katayama
Summary: Intestinal mucous layers play a crucial role in mediating the symbiotic and dysbiotic interactions between hosts and microbes. This study focuses on the mucin O-glycan degrading ability of gut microbes, specifically Bifidobacterium bifidum, and reveals the important roles of two 11-N-acetylglucosaminidases belonging to the GH20 and GH84 families in mucin O-glycan degradation. Substrate specificity analysis and O-glycomic analysis demonstrate the specific functions of BbhI and BbhIV in breaking down different types of mucin core structures. Additionally, the study uncovers the interdependence of BbhI and BbhIV in efficient hydrolysis of mucin core structures.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Toshihiko Katoh, Chihaya Yamada, Michael D. Wallace, Ayako Yoshida, Aina Gotoh, Moe Arai, Takako Maeshibu, Toma Kashima, Arno Hagenbeek, Miriam N. Ojima, Hiromi Takada, Mikiyasu Sakanaka, Hidenori Shimizu, Keita Nishiyama, Hisashi Ashida, Junko Hirose, Maria Suarez-Diez, Makoto Nishiyama, Ikuo Kimura, Keith A. Stubbs, Shinya Fushinobu, Takane Katayama
Summary: The BbhII enzyme from Bifidobacterium bifidum is involved in the breakdown of mucin O-glycans, modulating host-microbiota symbiosis and dysbiosis.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Chemistry, Medicinal
Shinsuke Inuki, Hitomi Tabuchi, Chiaki Matsuzaki, Yasunori Yonejima, Keiko Hisa, Ikuo Kimura, Kenji Yamamoto, Hiroaki Ohno
Summary: Exopolysaccharides (EPSs) are widely present in natural products made by bacteria, fungi, and algae, and some of them have interesting biological properties such as anticancer and immunomodulatory activities. Our recent study found that EPSs derived from Leuconostoc mesenteroides ssp. mesenteroides strain NTM048 enhanced the production of mucosal immunoglobulin A (IgA) in mice. In this study, we described the synthesis and evaluation of tetrasaccharide fragments of NTM048 EPS to gain insights into the molecular mechanism responsible for the induction of IgA.
CHEMICAL & PHARMACEUTICAL BULLETIN
(2022)
Article
Gastroenterology & Hepatology
Chiaki Matsuzaki, Yukari Nakashima, Ikuto Endo, Yusuke Tomabechi, Yasuki Higashimura, Saki Itonori, Koji Hosomi, Jun Kunisawa, Kenji Yamamoto, Keiko Hisa
Summary: Leuconostoc mesenteroides strain NTM048 produces an exopolysaccharide with adjuvanticity for mucosal vaccination, and the key polymer structure for adjuvanticity was determined to be Gtf2-S-glucan. Gtf2-S-glucan, produced in the supernatant by Gtf2, showed a higher ability to stimulate IgA production and induced higher antigen-specific antibodies in the airway mucosa and plasma compared to native NTM048 EPS. These findings suggest a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS.
Review
Biochemistry & Molecular Biology
Toshihiko Katoh, Kenji Yamamoto
Summary: The structure of glycans in glycoproteins directly affects their biological aspects, such as binding affinity. Recent progress in glycan remodeling and glycoengineering techniques has led to the development and production of biopharmaceutical glycan-modified antibodies with well-defined and homogeneous glycoforms.
JOURNAL OF APPLIED GLYCOSCIENCE
(2021)
