期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 63, 期 22, 页码 5381-5387出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.5b01335
关键词
lipid fraction; totally correlated NMR spectroscopy (TOCSY); spin chromatography; dairy products
资金
- Cyprus Research Promotion Foundation
- European Regional Development Fund
- Charalambides Christies, Ltd. dairy industry
We report a rapid, direct, and unequivocal spin-chromatographic separation and identification of minor components in the lipid fraction of milk and common dairy products with the use of selective one-dimensional (1D) total correlation spectroscopy (TOCSY) nuclear magnetic resonance (NMR) experiments. The method allows for the complete backbone spin-coupling network to be elucidated even in strongly overlapped regions and in the presence of major components from 4 x 10(2) to 3 x 10(3) stronger NMR signal intensities. The proposed spin-chromatography method does not require any derivatization steps for the lipid fraction, is selective with excellent resolution, is sensitive with quantitation capability, and compares favorably to two-dimensional (2D) TOCSY and gas chromatographymass spectrometry (GC-MS) methods of analysis. The results of the present study demonstrated that the 1D TOCSY NMR spin-chromatography method can become a procedure of primary interest in food analysis and generally in complex mixture analysis.
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