期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 63, 期 37, 页码 8216-8224出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.5b03138
关键词
Astragalus membranaceus; hairy root cultures; ultraviolet elicitation; gene expression; isoflavonoid biosynthesis; antioxidant activity
资金
- Special Fund of Forestry Industrial Research for Public Welfare of China [201004079]
- Application Technology Research and Development Program of Harbin [2013AA3BS014]
- Fundamental Research Funds for the Central Universities [2572014AA06, 2572015AA14]
- Special Fund of the National Natural Science Foundation of China [31270618]
- Key Program of the National Natural Science Foundation of China [81274010]
- Heilongjiang Province Outstanding Youth Fund [JC201101]
In this work, Astragalus membranaceus hairy root cultures (AMHRCs) were exposed to ultraviolet radiation (UV-A, UV-B, and UV-C) for promoting isoflavonoid accumulation. The optimum enhancement for isoflavonoid production was achieved in 34-day-old AMHRCs elicited by 86.4 kJ/m(2) of UV-B. The resulting isoflavonoid yield was 533.54 +/- 13.61 mu g/g dry weight (DW), which was 2.29-fold higher relative to control (232.93 +/- 3.08 mu g/g DW). UV-B up-regulated the transcriptional expressions of all investigated genes involved in isoflavonoid biosynthetic pathway. PAL and C4H were found to be two potential key genes that controlled isoflavonoid biosynthesis. Moreover, a significant increase was noted in antioxidant activity of extracts from UV-B-elicited AMHRCs (IC50 values = 0.85 and 1.08 mg/mL) in comparison with control (1.38 and 1.71 mg/mL). Overall, this study offered a feasible elicitation strategy to enhance isoflavonoid accumulation in AMHRCs and also provided a basis for metabolic engineering of isoflavonoid biosynthesis in the future.
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