Article
Veterinary Sciences
Jennifer Bencze-Nagy, Patrik Strifler, Boglarka Horvath, Nikoletta Such, Valeria Farkas, Karoly Dublecz, Laszlo Pal
Summary: The study aimed to investigate the health protective effects of dietary milk thistle seed, oil, and seed cake in ducks fed diets contaminated with deoxynivalenol and zearalenone. The results showed that milk thistle oil had a stronger positive effect on histopathology parameters compared to milk thistle seed and seed cake. All treatments were equally effective in reducing solitary cell death and infiltration of lympho- and histiocytes in the liver, as well as preventing lymphocyte depletion in the spleen and bursa of Fabricius. Overall, the applied milk thistle supplements were proven effective in preventing histopathological changes caused by deoxynivalenol and zearalenone.
VETERINARY SCIENCES
(2023)
Article
Chemistry, Applied
Chenyu Jiang, Xinghe Zhang, Jiahui Yu, Tinglan Yuan, Pu Zhao, Guanjun Tao, Wei Wei, Xingguo Wang
Summary: A new lipidomic method using UPSFC-Q-TOF-MS was proposed in this study, enabling the rapid separation of different types of polar lipids in milk. After analyzing polar lipids in seven samples, 14 lipid classes containing 219 lipid molecular species were identified.
Article
Biochemical Research Methods
C. Soto, H. D. Ponce-Rodriguez, J. Verdu-Andres, R. Herraez-Hernandez, P. Campins-Falco
Summary: In this study, three miniaturized liquid chromatography instruments were evaluated for analyzing caffeine in dietary supplements. The benchtop systems showed superior sensitivity, with capillary LC and nano LC systems having lower limits of detection compared to the portable LC system. The portable LC method was simpler and faster, while still providing accurate quantification of caffeine content in the samples.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemistry & Molecular Biology
Camila Soto, Henry Daniel Ponce-Rodriguez, Jorge Verdu-Andres, Pilar Campins-Falco, Rosa Herraez-Hernandez
Summary: With the growth of the dietary supplements industry, there is a demand for simplified and easy-to-use instruments. This study tested a hand-portable miniaturized liquid chromatograph for the determination of chlorogenic acids. The results showed that the portable LC instrument had comparable analytical performance and faster speed for quantification.
Article
Food Science & Technology
Oktawia Kalisz, Sylwia Studzinska, Szymon Bocian
Summary: Caffeine is a natural psychoactive substance found in various plants such as coffee, tea, cocoa, guarana, and yerba mate. It is commonly added to dietary supplements for its metabolism-boosting and weight loss benefits. A novel UHPLC method using environmentally friendly solvents was developed to determine the caffeine content in dietary supplements.
Article
Nutrition & Dietetics
Adal Mena-Garcia, Angie Julieth Bellaizac-Riascos, Maite Rada-Mendoza, Diana Maria Chito-Trujillo, Ana Isabel Ruiz-Matute, Maria Luz Sanz
Summary: Food supplements of plant origin, especially those containing (-)-hydroxycitric acid ((-)-HCA) from Garcinia cambogia, are increasingly popular among consumers for weight control and good health. However, recent controversies over their safety have raised concerns, requiring further research and monitoring of their composition. This study utilized a multi-analytical approach to determine the (-)-HCA content and other constituents in Garcinia cambogia food supplements, as well as detecting fraudulent practices in these products by comparing declared and experimentally determined values. The findings highlight the importance of quality control and the need for accurate labeling in these supplements.
Article
Food Science & Technology
Gabriela Pilarska, Magdalena Twaruzek, Iwona Altyn
Summary: This study aims to evaluate the contamination of molds and mycotoxins in dietary supplements based on purple coneflower in the Polish market. The results show that these supplements are commonly contaminated with molds and mycotoxins, with Aspergillus spp., Phoma spp., and Eurotium spp. being the most common molds, and ZEN, DON, and T-2 toxin being the most common mycotoxins.
Review
Biochemistry & Molecular Biology
Ancuta Cristina Raclariu-Manolica, Carmen Socaciu
Summary: Milk thistle is a popular botanical used for liver diseases, with silymarin as its main bioactive component. However, commercial preparations may not contain the recommended concentrations of silymarin, and may also contain toxic contaminants and foreign matters. This review discusses the extraction techniques of milk thistle metabolites and the quality, efficacy, and safety of derived food supplements.
Article
Chemistry, Analytical
Missael Antonio Arroyo Negrete, Kazimierz Wrobel, Eunice Yanez Barrientos, Alma Rosa Corrales Escobosa, Israel Enciso Donis, Katarzyna Wrobel
Summary: In this study, flow injection analysis (FIA) was used as a sample introduction method for an electrospray ionization - ion trap mass spectrometer (ESI-ITMS) to quantify CrPic(3) in commercial supplements. The FIA system operated with ammonium formate as a carrier solution, and cobalt picolinate was used as an internal standard (IS). The results showed that the proposed FIA-ESI-ITMS/MS procedure is a suitable method for the determination of CrPic(3) in such products.
Article
Food Science & Technology
Thammaporn Junsai, Saranya Poapolathep, Samak Sutjarit, Mario Giorgi, Zhaowei Zhang, Antonio Francesco Logrieco, Peiwu Li, Amnart Poapolathep
Summary: This study investigated the occurrence of multi-mycotoxins in six types of vegetable oils available in Thailand, revealing variations in mycotoxin contamination among different oil samples. The levels of analyzed mycotoxins were found to be below regulatory limits, indicating a low health risk to consumers.
Article
Environmental Sciences
Jakub Tomasko, Vojtech Hrbek, Tomas Kourimsky, Michal Stupak, Jana Hajslova, Jana Pulkrabova
Summary: Chlorinated paraffins (CPs) are emerging environmental pollutants associated with adverse effects on human health. This study detected CPs in fish oil-based dietary supplements and found high levels that could significantly impact certain population groups.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Review
Food Science & Technology
Karen Vanessa Marimon Sibaja, Sabrina De Oliveira Garcia, Wesclen Vilar Nogueira, Francine Kerstner de Oliveira, Eliana Badiale-Furlong, Jaqueline Garda-Buffon
Summary: The study examined occurrences of AFM1 in milk and dairy products in Latin America from 2003 to 2018, revealing high levels of incidence and concentration. Brazil, Costa Rica, Colombia, and Mexico had the highest estimated daily intakes of AFM1. Over the 15-year period, the average intake values in Latin American countries exceeded recommended levels.
FOOD REVIEWS INTERNATIONAL
(2022)
Article
Food Science & Technology
Xin Guan, Yuchao Feng, Decheng Suo, Zhiming Xiao, Shi Wang, Ying Liang, Xia Fan
Summary: In this study, a method for detecting 11 mycotoxins in maize was established using multiple-impurity adsorption purification (MIA) technologies and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The validated method was suitable for simultaneous determination of multiple mycotoxins in maize.
Article
Biochemical Research Methods
Quentin Gros, Marta Wolniaczyk, Johanna Duval, Shinnosuke Horie, Yasuhiro Funada, Yoshihiro Hayakawa, Caroline West, Eric Lesellier
Summary: Plant seeds, especially milk thistle seeds, contain valuable nonpolar and polar compounds with potential biological activity. This study developed an online method called supercritical fluid extraction (SFE)-supercritical fluid chromatography (SFC) to extract and analyze both types of compounds. Different transfer modes were compared, and the challenges of analyzing nonpolar and polar compounds were discussed. The versatility of online SFE-SFC and its potential in analyzing both types of compounds in a single sample were explored.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Masahiko Tachi, Shunya Kobayashi, Koji Tomita, Takashi Tanahashi, Susumu Y. Imanishi
Summary: In this study, a rapid and reliable screening method for PDE-5 inhibitors and their structural analogues was developed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and hierarchical clustering based on similarity of MS/MS spectra. The method was able to detect and distinguish various PDE-5 inhibitors and newly synthesized analogues. It was successfully applied to analyze herbal samples and dietary supplements, allowing for the detection of adulterant PDE-5 inhibitors such as sildenafil and tadalafil.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Review
Food Science & Technology
S. A. Tittlemier, J. Brunkhorst, B. Cramer, M. C. DeRosa, V. M. T. Lattanzio, R. Malone, C. Maragos, M. Stranska, M. W. Sumarah
Summary: This review summarizes recent developments in the analysis of mycotoxins in various matrices from mid-2019 to mid-2020, covering aspects from sampling and quality control to detection and quantitation technologies. The discussion mainly focuses on the significant advances in different technologies such as high resolution mass spectrometry, tandem mass spectrometry, and biosensors.
WORLD MYCOTOXIN JOURNAL
(2021)
Correction
Chemistry, Applied
Elizabet Janic Hajnal, Jovana Kos, Alexandra Malachova, David Steiner, Milena Stranska, Rudolf Krska, Michael Sulyok
Correction
Chemistry, Applied
Jovana Kos, Elizabet Janic Hajnal, Alexandra Malachova, David Steiner, Milena Stranska, Rudolf Krska, Birgit Poschmaier, Michael Sulyok
Article
Biochemical Research Methods
Milada Vitova, Milena Stranska, Andrea Palyzova, Tomas Rezanka
Summary: Cardiolipins are widely present in various organisms, and significant differences in molecular species were found among different organisms. The cleavage products of CLs also varied between organisms.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemistry & Molecular Biology
Milena Stranska, Petra Lovecka, Blanka Vrchotova, Leos Uttl, Kamila Bechynska, Adam Behner, Jana Hajslova
Summary: This study aimed to characterize the activities of endophytic consortia in V. vinifera through measuring five indicators and analyzing the metabolites upregulated with heavy endophyte colonization. The identified metabolites included chlorins, phenolics, flavonoid and terpenoid glycosides, tannins, dihydropyranones, sesquiterpene lactones, and long-chain unsaturated fatty acids.
CHEMISTRY & BIODIVERSITY
(2021)
Article
Chemistry, Applied
Milena Stranska, Leos Uttl, Kamila Bechynska, Kamila Hurkova, Adam Behner, Jana Hajslova
Summary: The study demonstrated the potential of metabolomic fingerprinting for characterizing grapevine leaves and canes, with harvesting season explaining most of the variability between samples, followed by different localities and farming systems which also influenced the abundance of metabolites.
Article
Chemistry, Applied
Nela Prusova, Zbynek Dzuman, Lukas Jelinek, Marcel Karabin, Jana Hajslova, Michael Rychlik, Milena Stranska
Summary: The study investigated the fate of less investigated Fusarium and Alternaria mycotoxins during the malting and brewing processes of producing lager beer. Results showed that germination is the most determining malting step, and mitigation measures are needed to reduce mycotoxins contamination in the final beer product.
Article
Food Science & Technology
Milena Stranska, Zbynek Dzuman, Nela Prusova, Adam Behner, Irena Kolouchova, Petra Lovecka, Tomas Rezanka, Miroslav Kolarik, Jana Hajslova
Summary: Fungal endophytes in grapevine have the potential to produce various mycotoxins, with verruculogen and gliotoxin being the most common and concentrated. It is important to carefully check the toxinogenic potential of endophytes when cultivating grapevines.
Review
Food Science & Technology
S. A. Tittlemier, B. Cramer, C. Dall'Asta, M. C. DeRosa, V. M. T. Lattanzio, R. Malone, C. Maragos, M. Stranska, M. W. Sumarah
Summary: This review summarizes recent developments in the analysis of mycotoxins in various matrices from mid-2020 to mid-2021. It covers different aspects of mycotoxin analysis, including sampling, quality control, and detection technologies. The review discusses important findings in chromatography, mass spectrometry, biosensors, and alternative assays. It also addresses limitations of the presented methodologies.
WORLD MYCOTOXIN JOURNAL
(2022)
Meeting Abstract
Cardiac & Cardiovascular Systems
Martin Maly, Jitka Viktorova, Tomas Ruml, Milena Stranska
CARDIOVASCULAR RESEARCH
(2022)
Article
Food Science & Technology
Nela Prusova, Adam Behner, Zbynek Dzuman, Jana Hajslova, Milena Stranska
Summary: Currently, there is increasing concern regarding the presence of conjugated mycotoxins in plant-based foods, however, the lack of analytical standards and occurrence data has hampered risk assessment processes. We developed a method for efficient pre-concentration of type A trichothecene glycosides, and identified their presence in oat-based products using high-resolution mass spectrometry. The results showed that glycosylated mycotoxins, particularly HT-2, accounted for a significant proportion of total mycotoxin content.
Article
Food Science & Technology
Milena Stranska, Nela Prusova, Adam Behner, Zbynek Dzuman, Michal Lazarek, Anna Tobolkova, Jana Chrpova, Jana Hajslova
Summary: The study investigates the effect of pulsed electric field (PEF) on mycotoxins present on malting barley. A reduction in trichothecenes, zearalenone, enniatins, beauvericin, and tentoxin was observed after PEF treatment. Increases in extraction of type A trichothecenes, zearalenone, enniatins, and beauvericin were also observed, possibly due to facilitated extraction from the matrix. Degradation/transformation products of mycotoxins were mainly the result of hydrolysis, elimination, and/or oxidation.
Review
Food Science & Technology
S. A. Tittlemier, B. Cramer, M. C. DeRosa, V. M. T. Lattanzio, R. Malone, C. Maragos, M. Stranska, M. W. Sumarah
Summary: This review summarizes the developments in mycotoxin analysis from mid-2021 to mid-2022, covering sampling, quality control, and various detection technologies. It discusses chromatography, biosensors, and alternative assays, as well as limitations of the presented methodologies. The purpose is to guide readers to relevant research and provide a comprehensive assessment of the analytical developments.
WORLD MYCOTOXIN JOURNAL
(2023)
Article
Biochemical Research Methods
Matej Maly, Frantisek Benes, Zuzana Binova, Marie Zlechovcova, Petr Kastanek, Jana Hajslova
Summary: This study demonstrates the applicability of fast centrifugal partition chromatography (FCPC) as an effective method for the isolation of CBD and CBDA free of psychotropic compounds from Cannabis sativa L. plant extracts. The purity of isolated CBD and CBDA reached 98.9% and 95.1% (w/w), respectively, without the presence of non-psychotropic compounds, with only trace amounts of other biologically active compounds detected.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Lukas Vaclavik, John Schmitz, Matthew Eckert, Katerina Mastovska, Tarun Anumol
Summary: This article summarizes optimization experiments and validation of a complete workflow for the determination of PFAS in food using a liquid chromatography-tandem mass spectrometry method. PFAS are a group of anthropogenic chemicals that have become global environmental pollutants due to their toxicity and persistence. Recently, there has been increased interest in the risk to consumers related to the presence of PFAS in foods.
LCGC NORTH AMERICA
(2022)
