Genetic and chemical characterization of an EMS induced mutation in Cucumis melo CRTISO gene

In order to broaden the available genetic variation of melon, we developed an ethyl methanesulfonate mutation library in an orange-flesh 'Charentais' type melon line that accumulates beta-carotene. One mutagenized M-2 family segregated for a novel recessive trait, a yellow-orange fruit flesh ('yofl'). HPLC analysis revealed that 'yofl' accumulates pro-lycopene (tetra-cis-lycopene) as its major fruit pigment. The altered carotenoid composition of 'yofl' is associated with a significant change of the fruit aroma since cleavage of beta-carotene yields different apocarotenoids than the cleavage of pro-lycopene. Normally, pro-lycopene is further isomerized by CRTISO (carotenoid isomerase) to yield all-trans-lycopene, which is further cyclized to beta-carotene in melon fruit. Cloning and sequencing of 'yofl' CRTISO identified two mRNA sequences which lead to truncated forms of CRTISO. Sequencing of the genomic CRTISO identified an A-T transversion in which leads to a premature STOP codon. The early carotenoid pathway genes were up regulated in 'yofl' fruit causing accumulation of other intermediates such as phytoene and zeta-carotene. Total carotenoid levels are only slightly increased in the mutant. Mutants accumulating pro-lycopene have been reported in both tomato and watermelon fruits, however, this is the first report of a non-lycopene accumulating fruit showing this phenomenon. (c) 2013 Elsevier Inc. All rights reserved.