Article
Fisheries
Heng-Chih Pan, Kang-Chieh Lo, Yun-Hsin Wang, Han-Ting Huang, Shu-Chun Cheng, Chiao-Yin Sun, Yau-Hung Chen
Summary: The results of this study indicate that klotho protein deficiency impairs cell movement during early fish development and affects gene expression.
Article
Cell Biology
Chang Liu, Rui Li, Young Li, Xiumei Lin, Kaichen Zhao, Qun Liu, Shuowen Wang, Xueqian Yang, Xuyang Shi, Yuting Ma, Chenyu Pei, Hui Wang, Wendai Bao, Junhou Hui, Tao Yang, Zhicheng Xu, Tingting Lai, Michael Arman Berberoglu, Sunil Kumar Sahu, Miguel A. Esteban, Kailong Ma, Guangyi Fan, Yuxiang Li, Shiping Liu, Ao Chen, Xun Xu, Zhiqiang Dong, Longqi Liu
Summary: This study used Stereo-seq and scRNA-seq data to analyze the spatially resolved developmental trajectories of cell-fate transitions and molecular changes during zebrafish embryogenesis. Important interactions were identified, providing a fundamental reference for further studies on vertebrate development.
DEVELOPMENTAL CELL
(2022)
Article
Cell Biology
Ting Zhao, Shengfan Ye, Zimu Tang, Liwei Guo, Zhipeng Ma, Yuxi Zhang, Chun Yang, Jinrong Peng, Jun Chen
Summary: Delta 133p53 plays a protective role against cellular senescence by upregulating antioxidant genes expression, and its decreased expression in brain tissues of AD and ALS patients has been observed. The zebrafish ortholog Delta 113p53 contributes to neurogenesis and brain function maintenance, with loss of cognitive ability observed in mutants but not in wild-type fish at older ages.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Ivanildo Inacio da Silva Jr, Niely Priscila Correia da Silva, James A. Marrs, Pabyton Goncalves Cadena
Summary: This study evaluated the effects of lemongrass, thyme, and oregano essential oils on zebrafish embryos and larvae, and found that these oils can influence the development and cause morphological defects and behavioral changes in the embryos and larvae. Essential oil exposure may have neurodevelopmental toxicity on zebrafish. Therefore, careful use of essential oils, especially during pregnancy, should be considered.
Article
Biochemistry & Molecular Biology
Khadizatul Kubra, Gurveer K. Gaddu, Clifford Liongue, Somayyeh Heidary, Alister C. Ward, Amardeep S. Dhillon, Faiza Basheer
Summary: Members of the FOS protein family play important roles in regulating gene expression and are involved in pathological conditions. Using zebrafish as a model, this study found that zebrafish FOS orthologs exhibit distinct spatiotemporal expression patterns during embryonic development and in adult tissues, and are regulated by oncogenic signals. This suggests that zebrafish can serve as an alternate model for studying the regulation and functions of Fos proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Junren Zhang, Yang Zhou, Shuang Li, Dashuang Mo, Jianlong Ma, Rui Ni, Qifen Yang, Jianbo He, Lingfei Luo
Summary: In this study, a liver regeneration mutant, liver logan (lvl), was identified in a zebrafish model, with a mutation in the telomere maintenance 2 (tel2) gene. The tel2 mutation specifically inhibits the transcriptional activation of a hepatoblast marker, hematopoietically expressed homeobox (hhex), in biliary epithelial cell (BEC)-derived cells during liver regeneration, blocking the redifferentiation of bipotential progenitor cells (BP-PCs). Mechanistic studies reveal that Tel2 associates with the hhex promoter region and promotes hhex transcription.
Article
Behavioral Sciences
Jaquelinne Pinheiro-da-Silva, Ana Carolina Luchiari
Summary: Ethanol exposure during embryogenesis can have harmful effects on zebrafish, leading to reduced body size, decreased heartbeat rate, increased apoptotic cell numbers, and differences in hatching time. This study highlights the zebrafish as a suitable model for studying fetal alcohol spectrum disorders (FASD).
BRAIN AND BEHAVIOR
(2021)
Article
Biotechnology & Applied Microbiology
Maxime Fages-Lartaud, Lisa Tietze, Florence Elie, Rahmi Lale, Martin Frank Hohmann-Marriott
Summary: In this study, we discovered a problem in the red fluorescent protein family, where a short protein isoform produces significant background fluorescence that affects the interpretation of experimental results. We also provided an improved tool for imaging and protein expression studies.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Kane Toh, Dillan Saunders, Berta Verd, Benjamin Steventon
Summary: This study supports the existence of the transition state model of cell differentiation by observing gene expression variability in zebrafish neuromesodermal progenitors. The results show that there is a transient window of gene expression stochasticity before entry into a differentiated state, and cell population heterogeneity peaks during this period.
Review
Biochemistry & Molecular Biology
Gaia Pucci, Giusi Irma Forte, Vincenzo Cavalieri
Summary: Radiotherapy is not yet personalized in cancer treatment plans, as its effectiveness varies depending on the radiosensitivity of tumor cells. Research is focusing on enhancing cancer cell response to ionizing radiation and protecting healthy tissues, using zebrafish as an effective model system for testing radiation modifiers for potential radiotherapeutic purposes in humans.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Tarannum Taznin, Kaushalya Perera, Yann Gibert, Alister C. Ward, Clifford Liongue
Summary: This study investigated the expression and function of CRLF3, an evolutionarily significant protein, in early development, particularly in blood and immune cells, using zebrafish as a model. The study found that CRLF3 was expressed in multiple embryonic tissues and played a crucial role in primitive hematopoiesis and early definitive hematopoiesis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biology
Floyd Hassenrueck, Maria Farina-Morillas, Lars Neumann, Francesco Landini, Stuart James Blakemore, Mina Rabipour, Juan Raul Alvarez-Idaboy, Christian P. Pallasch, Michael Hallek, Rocio Rebollido-Rios, Guenter Krause
Summary: Cell-based and molecular exploration provides comparative characterization of currently developed PI3K inhibitors and structural insights for future inhibitor design. The study focuses on targeting the PI3K isoform p110 delta against B cell malignancies and evaluates the potency, isoform-selectivity, and molecular interactions of various PI3Ki chemotypes. The I777M substitution affects the efficacy and selectivity of p110 delta-selective inhibitors, but not multi-targeted inhibitors, due to its effect on the conformational flexibility of p110 delta's specificity or affinity pockets. This research provides valuable information for optimizing PI3Ki design.
COMMUNICATIONS BIOLOGY
(2023)
Article
Oncology
Xiajie Zhang, Kira Groen, Brianna C. Morten, Luiza Steffens Reinhardt, Hamish G. Campbell, Antony W. Braithwaite, Jean-Christophe Bourdon, Kelly A. Avery-Kiejda
Summary: This study aimed to evaluate the role of Delta 40p53 in breast cancer migration and invasion, finding an association between Delta 40p53 and gene expression profiles. Knockdown of both p53 alpha and Delta 40p53 resulted in increased proliferation, while overexpression of Delta 40p53 reduced proliferation rates and decreased migratory and invasive properties of the cells. These results suggest that at a basal level, Delta 40p53 works similarly to p53 alpha in suppressing cellular mobility and proliferation.
MOLECULAR ONCOLOGY
(2022)
Article
Medicine, Research & Experimental
Andrew R. Findlay, May M. Paing, Jil A. Daw, Meade Haller, Rocio Bengoechea, Sara K. Pittman, Shan Li, Feng Wang, Timothy M. Miller, Heather L. True, Tsui-Fen Chou, Conrad C. Weihl
Summary: Dominant missense mutations in DNAJB6 cause limb girdle muscular dystrophy (LGMD) D1, but there are currently no treatments available. We developed an isoform-specific knockdown approach using morpholinos to selectively reduce each isoform in vitro and in vivo. Selective reduction of DNAJB6b levels in LGMD D1 myotubes corrected much of the disease signature related to protein homeostasis and myofibril structure. Additional in vivo functional data is needed to determine if selective reduction of DNAJB6b is a viable therapeutic target for LGMD D1.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Article
Biochemistry & Molecular Biology
Jong-Chan Ahn, Su Jung Hwang, Hyo-Jong Lee, Kyu-Won Kim
Summary: The study showed that claudin-5a is crucial for establishing and maintaining the blood-neural barrier during zebrafish development. Knockdown of claudin-5a resulted in selective leakage through the BBB, decreased expression of glucose transporter 1 in cerebral microvessels, and leakiness in the blood-cerebrospinal fluid barrier, indicating overall abnormal development of the blood-neural barriers.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yukako Senga, Atsuhiko Ishida, Yasushi Shigeri, Isamu Kameshita, Noriyuki Sueyoshi
Article
Biochemical Research Methods
Yasunori Sugiyama, Sho Yamashita, Yuuki Uezato, Yukako Senga, Syouichi Katayama, Naoki Goshima, Yasushi Shigeri, Noriyuki Sueyoshi, Isamu Kameshita
ANALYTICAL BIOCHEMISTRY
(2016)
Article
Biochemistry & Molecular Biology
Yukako Senga, Kazutoshi Akizuki, Syouichi Katayama, Yasushi Shigeri, Isamu Kameshita, Atsuhiko Ishida, Noriyuki Sueyoshi
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2016)
Article
Genetics & Heredity
Syouichi Katayama, Yukako Senga, Arni Oi, Yosuke Miki, Yasunori Sugiyama, Noriyuki Sueyoshi, Isamu Kameshita
Review
Chemistry, Medicinal
Kiyohito Yoshida, Mikako Hashimoto, Ryuji Hori, Takumi Adachi, Hidetoshi Okuyama, Yoshitake Orikasa, Tadashi Nagamine, Satoru Shimizu, Akio Ueno, Naoki Morita
Article
Soil Science
Nan Gao, Weishou Shen, Hiroko Kakuta, Nobuhiro Tanaka, Toru Fujiwara, Tomoyasu Nishizawa, Noriko Takaya, Tadashi Nagamine, Kazuo Isobe, Shigeto Otsuka, Keishi Senoo
SOIL BIOLOGY & BIOCHEMISTRY
(2016)
Article
Biochemistry & Molecular Biology
Masashi Yamashita, Noriyuki Sueyoshi, Hiroki Yamada, Syouichi Katayama, Yukako Senga, Yasuhiro Takenaka, Atsuhiko Ishida, Isamu Kameshita, Yasushi Shigeri
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2018)
Article
Biochemical Research Methods
Yukako Senga, Shinya Honda
BIOCONJUGATE CHEMISTRY
(2018)
Article
Multidisciplinary Sciences
Yukako Senga, Hiroshi Imamura, Takamitsu Miyafusa, Hideki Watanabe, Shinya Honda
SCIENTIFIC REPORTS
(2017)
Article
Chemistry, Analytical
Yukako Senga, Hiroshi Imamura, Toshihiko Ogura, Shinya Honda
ANALYTICAL CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Tatsuto Nakane, Ayae Ido, Takuma Higuchi, Hiroshi Todaka, Keiko Morisawa, Tadashi Nagamine, Kensaku Fukunaga, Shuji Sakamoto, Koji Murao, Yasunori Sugiyama
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2019)
Article
Biotechnology & Applied Microbiology
Nobuo Fukuda, Yukako Senga, Shinya Honda
BIOTECHNOLOGY PROGRESS
(2019)
Article
Biochemistry & Molecular Biology
Kazutoshi Akizuki, Tomoya Kinumi, Ayaka Ono, Yukako Senga, Jin Osawa, Yasushi Shigeri, Atsuhiko Ishida, Isamu Kameshita, Noriyuki Sueyoshi
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2019)
Article
Microbiology
Takehiko Sahara, Takumi Adachi, Tadashi Nagamine, Toru Fukano, Naoki Morita
Summary: We sequenced the draft genome of thraustochytrid strain 12B, which exhibits high production of polyunsaturated fatty acids like docosahexaenoic acid. The draft genome sequence, approximately 65 Mbp, will provide insights into metabolic engineering for enhancing the production of polyunsaturated fatty acids in this microorganism.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Biochemistry & Molecular Biology
Yukako Senga, Motomichi Doi, Masayoshi Onitsuka, Shinya Honda
Summary: This study establishes an approach to detect intracellular aggregates using a fluorescent-labeled artificial protein, AF.2A1, and finds that the relative amount of intracellular aggregates is correlated with the type of additives used during cell culture. Additionally, there is a moderate correlation between the relative amount of intracellular aggregates and that of extracellular aggregates, suggesting the secretion of intracellular aggregates.
CELL CHEMICAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yuri Nagaoka, Kotone Oshiro, Yuta Yoshino, Toshiyuki Matsunaga, Satoshi Endo, Akira Ikari
Summary: This study investigated the effect of intercellular adhesion molecule CLDN1 on the anticancer drug sensitivity of small-cell lung cancer (SCLC) cells. It was found that overexpression of CLDN1 decreased the sensitivity of SCLC cells to anticancer drugs and enhanced their migratory capacity through the activation of the TGF-81/EMT signaling pathway. Treatment with EMT inhibitors showed potential in overcoming the reduced sensitivity to anticancer drugs in CLDN1-overexpressing SCLC cells.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2024)