Article
Pharmacology & Pharmacy
Shusheng Fan, Xiaofei Huang, Haowei Tong, Huitao Hong, Zhulan Lai, Wanting Hu, Xiaoyun Liu, Luyong Zhang, Zhenzhou Jiang, Qinwei Yu
Summary: The study demonstrates the crucial role of TAK1 phosphorylation in muscle regeneration, affecting myoblast proliferation and differentiation phases. By regulating the expression level of HO-1, muscle disease progression can be improved.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Gastroenterology & Hepatology
Zhaoyun Yang, Kang He, Taiwei Wang, Yan Gao, Mengmeng Chen, Kai Wang, Zeyu Wang, Hao Wu, Shuang Zhang, Lijing Zhao
Summary: This study found that mechanical dilation leads to the expression of COX-2 in colonic smooth muscle cells, causing motility dysfunction in obstructive bowel disorders. Inhibition of PKCs and PKD can improve this dysfunction by preventing the expression of COX-2.
NEUROGASTROENTEROLOGY AND MOTILITY
(2023)
Article
Multidisciplinary Sciences
Benedikt Jaeger, Jonas Christian Schupp, Linda Plappert, Oliver Terwolbeck, Gian Kayser, Peggy Engelhard, Taylor Sterling Adams, Robert Zweigerdt, Henning Kempf, Stefan Lienenklaus, Wiebke Garrels, Irina Nazarenko, Danny Jonigk, Malgorzata Wygrecka, Denise Klatt, Axel Schambach, Naftali Kaminski, Antje Prasse, Nataliia Artysh
Summary: This study focuses on the properties of airway basal cells (ABC) obtained from patients with idiopathic pulmonary fibrosis (IPF) and reveals the extensive reprogramming of IPF-ABC. The study also shows that IPF-ABC can induce fibroblast proliferation and extracellular matrix deposition in a 3D organoid model, and these changes can be reversed by SRC inhibition. The findings suggest that IPF-ABC and SRC inhibition may serve as potential targets for the treatment of IPF.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Anita Kneppers, Sabrina Ben Larbi, Marine Theret, Audrey Saugues, Carole Dabadie, Linda Gsaier, Arnaud Ferry, Philipp Rhein, Julien Gondin, Kei Sakamoto, Remi Mounier
Summary: This study reveals the crucial role of AMPK alpha 2 in coordinating events that support myonuclear accretion in skeletal muscle. By regulating the phosphorylation of the non-metabolic substrate BAIAP2, AMPK alpha 2 plays a specific role in MuSCs in the regulation of myonuclear accretion. The deletion of AMPK alpha 2 impairs skeletal muscle regeneration and decreases myonuclear accretion in response to myofiber contraction.
Review
Biochemistry & Molecular Biology
Yi Liu, Mengmeng Duan, Demao Zhang, Jing Xie
Summary: Mechano growth factor (MGF), a mechanically sensitive growth factor, plays a crucial role in the skeletal system and has high expression in chondrocytes in the joint cavity. It affects chondrocyte behavior and maintains cartilage homeostasis. Despite recent discoveries, its role in cartilage development and disease occurrence remains controversial and its internal biological mechanism is still unclear.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2023)
Review
Biochemistry & Molecular Biology
Duangnapa Kovanich, Teck Yew Low, Manuela Zaccolo
Summary: cAMP is a second messenger that plays a crucial role in regulating cellular functions. The compartmentalization of cAMP signaling is essential for its specificity, and the formation of dynamic signaling domains is responsible for precise spatiotemporal regulation. Proteomics can be used to identify the components of these domains and define the dynamic landscape of cellular cAMP signaling. Understanding compartmentalized cAMP signaling in physiological and pathological conditions can provide insights into disease mechanisms and guide the development of precision medicine interventions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Elia Mota, Segolene Bompierre, Dahdjim Betolngar, Liliana R. Castro, Pierre Vincent
Summary: This study reveals the functional contribution of PDE2A, PDE4, and PDE10A in regulating cAMP/PKA response to dopamine stimulation in the striatum. PDE10A is found to play a key role in reducing cAMP levels back to baseline and dephosphorylating PKA substrates. Inhibiting PDE10A dampens responsiveness to dopamine, while inhibiting PDE2A or PDE4 strengthens dopamine action.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Roberta Paolillo, Stefania D'Apice, Gabriele Giacomo Schiattarella, Pietro Ameri, Domenica Borzacchiello, Daniele Catalucci, Cristina Chimenti, Lia Crotti, Sebastiano Sciarretta, Daniele Torella, Antonio Feliciello, Cinzia Perrino
Summary: The second messenger cAMP regulates multiple mitochondrial functions through the activation of cAMP/PKA signaling. AKAPs play important roles in regulating cardiac functions. Understanding the roles and regulation of mitochondria-targeted AKAPs can help modulate their functions for clinical purposes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Shigeo Otake, Yuka Itoh, Chiho Omata, Masao Saitoh, Keiji Miyazawa
Summary: E-cadherin can be downregulated in KRAS-mutated cancer cells through a novel regulatory switch involving ZEB1 and oncogenic K-Ras, which requires an external cue for temporal downregulation. This regulatory mechanism is distinct from EPCAM repression by ZEB1 and contributes to phenotypic plasticity during malignant progression.
Article
Cell Biology
Armando Jesus Perez-Diaz, Beatriz Vazquez-Marin, Jero Vicente-Soler, Francisco Prieto-Ruiz, Teresa Soto, Alejandro Franco, Jose Cansado, Marisa Madrid
Summary: The autophagy pathway in yeast is regulated differently in response to glucose limitation, indicating evolutionary variations in the signaling pathways that govern autophagy during glucose shortage or starvation.
Article
Physiology
Christopher Solis, Walter C. C. Thompson, James R. R. Pena, Christopher McDermott-Roe, Paulina Langa, Chad M. M. Warren, Magdalena Chrzanowska, Beata M. M. Wolska, R. John Solaro, Pieter H. Detombe, Paul Goldspink
Summary: In this study, the function and regulation mechanism of MGF's E-domain were investigated. By analyzing the sequence and protein interactions, it was found that the phosphorylation state regulates the stability of 14-3-3 proteins and their interactions with relevant proteins. This has important implications for understanding the regulation of cardiac function.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yasemin Ozgur-Gunes, Catherine Le Stunff, Malha Chedik, Marie-Pierre Belot, Pierre-Hadrien Becker, Veronique Blouin, Pierre Bougneres
Summary: The gene therapy with hPRKAR1A improved skeletal growth and kidney dysfunction in acrodysostosis, but did not significantly affect the bone length of limbs.
Article
Multidisciplinary Sciences
Eva Crosas-Molist, Vittoria Graziani, Oscar Maiques, Pahini Pandya, Joanne Monger, Remi Samain, Samantha L. George, Saba Malik, Jerrine Salise, Valle Morales, Adrien Le Guennec, R. Andrew Atkinson, Rosa M. Marti, Xavier Matias-Guiu, Guillaume Charras, Maria R. Conte, Alberto Elosegui-Artola, Mark Holt, Victoria Sanz-Moreno
Summary: Cell migration is controlled by AMP-activated protein kinase (AMPK) acting as an adhesion sensing molecular hub. High AMPK activity in low adhering migratory cells induces mitochondrial fission and amoeboid migration. Reducing adhesion or activating AMPK promotes efficient rounded-amoeboid migration. This study reveals how mitochondrial dynamics and AMPK contribute to cell migration.
NATURE COMMUNICATIONS
(2023)
Editorial Material
Hematology
Jan A. Burger
Summary: The study reveals that MARCKS protein is differentially expressed in patients with chronic lymphocytic leukemia based on the mutation status of IGHV, and its expression and phosphorylation are linked to CLL cell migration through key signaling pathways. The findings were also confirmed in samples from patients treated with the BTK inhibitor acalabrutinib.
Article
Biochemistry & Molecular Biology
Falguni Das, Nandini Ghosh-Choudhury, Soumya Maity, Balakuntalam S. Kasinath, Goutam Ghosh Choudhury
Summary: The plasticity of proximal tubular epithelial cells contributes to renal fibrosis through the expression of TWIST1 in response to TGF beta. The mechanism of TWIST1 expression has not been clarified. This study demonstrates that both PI3 kinase and its target mTORC2 increase the expression of TWIST1 induced by TGF beta. Moreover, phosphorylation of the hydrophobic motif site Ser-660 in protein kinase C beta II (PKC beta II) is enhanced by TGF beta. Inhibition of TWIST1 arrests TGF beta-induced tubular cell pathology, while its overexpression induces these pathologies.
Article
Multidisciplinary Sciences
Tamara V. Tikhonova, Dimitry Y. Sorokin, Wilfred R. Hagen, Maria G. Khrenova, Gerard Muyzer, Tatiana V. Rakitina, Ivan G. Shabalin, Anton A. Trofimov, Stanislav I. Tsallagov, Vladimir O. Popov
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biochemistry & Molecular Biology
Irina Kravchenko, Vladimir A. Furalyov, Vladimir O. Popov
Article
Cell Biology
Egor M. Lednev, Irina Kravchenko, Vladimir A. Furalyov, Evgeny A. Lysenko, Iulia S. Lemesheva, AlexandrA Grushin, Vadim E. Dubrov, Olga L. Vinogradova, Daniil Popov
GROWTH HORMONE & IGF RESEARCH
(2020)
Article
Biochemical Research Methods
Arthur Boyarov, Alina Osmakova, Vladimir Popov
Summary: This article provides an overview of the implementation of the BIO 2020 program in the Russian Federation, covering driving factors of bioeconomic development, new measures of state regulation, and examples of projects in various biotechnology fields. The analysis suggests that these factors may have a significant impact on the Russian bioeconomy in the short to medium term.
Article
Microbiology
Sergey N. Gavrilov, Daria G. Zavarzina, Ivan M. Elizarov, Tamara V. Tikhonova, Natalia I. Dergousova, Vladimir O. Popov, Jonathan R. Lloyd, David Knight, Mohamed Y. El-Naggar, Sahand Pirbadian, Kar Man Leung, Frank T. Robb, Maksim V. Zakhartsev, Orianna Bretschger, Elizaveta A. Bonch-Osmolovskaya
Summary: The biogenic transformation of Fe minerals associated with extracellular electron transfer (EET) by thermophilic Gram-positive bacteria is not well understood. In this study, the EET pathways in the thermophilic Gram-positive bacterium Carboxydothermus ferrireducens were investigated, revealing the formation of magnetite crystals and active colonization of anodes in bioelectrochemical systems. Genome analysis identified novel c-type multiheme cytochromes involved in electron exchange with ferrihydrite or an anode, providing insights into cell-to-mineral interactions in thermal environments.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Konstantin M. Boyko, Mariya V. Kryukova, Lada E. Petrovskaya, Elena A. Kryukova, Alena Y. Nikolaeva, Dmitry A. Korzhenevsky, Galina Yu. Lomakina, Ksenia A. Novototskaya-Vlasova, Elizaveta M. Rivkina, Dmitry A. Dolgikh, Mikhail P. Kirpichnikov, Vladimir O. Popov
Summary: The novel cold-active esterase PMGL3, belonging to the HSL family, forms a unique tetrameric structure with significant impact on stability and catalysis demonstrated by mutant analysis.
Article
Biochemistry & Molecular Biology
Artem Bonchuk, Konstantin Boyko, Anna Fedotova, Alena Nikolaeva, Sofya Lushchekina, Anastasia Khrustaleva, Vladimir Popov, Pavel Georgiev
Summary: In arthropods, ZAD-C2H2 proteins form homodimers in Drosophila melanogaster, with only certain ZADs able to heterodimerize. The rapid evolutionary acquisition of interaction specificity in ZAD-C2H2 proteins is mediated by the more energy-favorable formation of homodimers compared to heterodimers. Specific homodimerization is crucial for the architectural role of ZAD-C2H2 proteins in genome organization.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Victor Andoralov, Sergey Shleev, Natalia Dergousova, Olga Kulikova, Vladimir Popov, Tamara Tikhonova
Summary: Detailed studies were conducted on the impedance and voltammetric behavior of hexameric octaheme nitrite reductase immobilized on carbon-based nanomaterials, specifically nanotubes and nanoparticles. The bioelectrocatalytic reduction of nitrite on enzyme-modified electrodes was well pronounced. Analysis of impedance data excluded the involvement of long-lived intermediates in the nitrite reduction process. The cyclic voltammograms of biomodified electrodes displayed a bi-sigmoidal shape, indicating the presence of two enzyme orientations on carbon supports. Furthermore, schemes for the rate-limiting processes during bioelectrocatalysis were proposed for low-and high-potential enzyme orientations.
BIOELECTROCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Alina K. Bakunova, Alena Yu. Nikolaeva, Tatiana V. Rakitina, Tatiana Y. Isaikina, Maria G. Khrenova, Konstantin M. Boyko, Vladimir O. Popov, Ekaterina Yu. Bezsudnova
Summary: This study elucidated a new D-amino acid transaminase with high specificity towards D-amino acids and their analogs, demonstrating unique active site residues compared to previously studied transaminases. The presence of critical residues for catalytic activity and the suggestion of arginine residues' functions based on structural analysis, mutagenesis, and modeling indicate a novel insight into the substrate specificity of this enzyme. The characteristics of Halhy contribute to our understanding of substrate specificity and structure-function relationships in D-amino acid transaminases.
Article
Multidisciplinary Sciences
Ekaterina Yu. Bezsudnova, Alena Yu. Nikolaeva, Alina K. Bakunova, Tatiana V. Rakitina, Dmitry A. Suplatov, Vladimir O. Popov, Konstantin M. Boyko
Summary: This study analyzed the relationship between structure and function of transaminase from Thermobaculum terrenum, creating a panel of enzyme variants through site-directed mutagenesis to achieve (R)-(+)-1-phenylethylamine selectivity while retaining specificity for traditional substrates. Spatial reorganization of active sites resulted in changes in activity, allowing for a better understanding of the role of specific active site residues in expanding substrate specificity.
Article
Biochemistry & Molecular Biology
Marat Sabirov, Anastasia Popovich, Konstantin Boyko, Alena Nikolaeva, Olga Kyrchanova, Oksana Maksimenko, Vladimir Popov, Pavel Georgiev, Artem Bonchuk
Summary: The study identified new CP190 partners and demonstrated that architectural proteins use various mechanisms to increase the efficiency of their interaction with CP190.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Oksana M. Subach, Anna V. Vlaskina, Yuliya K. Agapova, Pavel V. Dorovatovskii, Alena Y. Nikolaeva, Olga I. Ivashkina, Vladimir O. Popov, Kiryl D. Piatkevich, Maria G. Khrenova, Tatiana A. Smirnova, Konstantin M. Boyko, Fedor V. Subach
Summary: In this study, traditional bright red fluorescent proteins were converted into proteins with a large Stokes shift using rational design and random mutagenesis. These novel LSSRFPs were successfully developed and quantitatively evaluated for dual-color confocal imaging and two-photon imaging in mammalian cells. The X-ray structure of the protein and the excited-state proton transfer mechanism were also elucidated, along with the role of specific residue in pH stability and the unsuitability of the protein for STED microscopy due to photoconversion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Oksana M. Subach, Aleksandr Tashkeev, Anna V. Vlaskina, Dmitry E. Petrenko, Filipp A. Gaivoronskii, Alena Y. Nikolaeva, Olga I. Ivashkina, Konstantin Anokhin, Vladimir O. Popov, Konstantin M. Boyko, Fedor Subach
Summary: Genetically encoded monomeric blue-to-red fluorescent timers can change their fluorescent color over time, and have important applications in cell research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Crystallography
Konstantin M. Boyko, Ilya O. Matyuta, Alena Y. Nikolaeva, Tatiana Rakitina, Vladimir O. Popov, Ekaterina Yu Bezsudnova, Maria G. Khrenova
Summary: Effective biocatalysts for the synthesis of optically pure amines are highly demanded in organic synthesis. Previous bioinformatic studies identified transaminases with unusual characteristics in some Gram-negative bacteria. Structural analysis of the protein encoded by one of these genes from Variovorax paradoxus revealed a substitution of the catalytic lysine residue with asparagine. Despite this substitution, the protein was still able to bind PLP in a specific manner, suggesting potential hidden functional roles for such proteins.
Article
Chemistry, Multidisciplinary
Maria G. Khrenova, Anastasia Yu Soloveva, Larisa A. Varfolomeeva, Tamara Tikhonova, Vladimir O. Popov
Summary: According to steady-state kinetic experiments, thiourea inhibits thiocyanate dehydrogenase TcDH, whereas urea does not. The molecular mechanism revealed that upon binding, a sulfur atom forms stronger coordination bonds with copper ions than an oxygen for both compounds.
MENDELEEV COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Yuri Nagaoka, Kotone Oshiro, Yuta Yoshino, Toshiyuki Matsunaga, Satoshi Endo, Akira Ikari
Summary: This study investigated the effect of intercellular adhesion molecule CLDN1 on the anticancer drug sensitivity of small-cell lung cancer (SCLC) cells. It was found that overexpression of CLDN1 decreased the sensitivity of SCLC cells to anticancer drugs and enhanced their migratory capacity through the activation of the TGF-81/EMT signaling pathway. Treatment with EMT inhibitors showed potential in overcoming the reduced sensitivity to anticancer drugs in CLDN1-overexpressing SCLC cells.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2024)