期刊
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
卷 494, 期 1, 页码 101-105出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2009.11.023
关键词
Autophagy; Ceramide; Dihydroceramide; Photodynamic therapy
资金
- U.S. Public Health Service [R01 CA77475]
- National Cancer Institute, National Institutes of Health
The purpose of this study was to determine the sphingolipid (SL) profile in cells defective in autophagy protein ATG-7 and overall cell death after photodynamic therapy (PDT) with the photosensitizer Pc 4. MCF-7 human breast cancer cells with downregulated ATG-7 and their scrambled controls (Scr) were used. Exposure of ATG-7 knockdown cells to PDT led to increased cell killing. PDT evoked an early (2 h) greater global increase in ceramides in ATG-7 defective cells compared to Scr cells. The total increases in dihydroceramide (DHceramide) were significant at 2 and 24 h in both cell types post-PDT. The levels of sphingosine-1-phosphate (S1P) and sphingosine were decreased below resting levels at both time points irrespective of the cell type. The data imply that ceramide might be a marker of ATG-7 deficiency in cells sensitized to PDT. (C) 2009 Elsevier Inc. All rights reserved.
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