Article
Microbiology
Rebecca K. Donegan, Yibo Fu, Jacqueline Copeland, Stanzin Idga, Gabriel Brown, Owen F. Hale, Avishek Mitra, Hui Yang, Harry A. Dailey, Michael Niederweis, Paras Jain, Amit R. Reddi
Summary: This study found that Mycobacterium tuberculosis utilizes endogenous and exogenous heme differently, and targeting heme synthesis may be an effective therapeutic strategy to treat mycobacterial infections.
MICROBIOLOGY SPECTRUM
(2022)
Article
Cell Biology
Kamila Balusikova, Marketa Dostalikova-Cimburova, Ilja Tacheci, Jan Kovar
Summary: The expression of molecules involved in the absorption of heme and non-heme iron varies depending on the location in the duodenum.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Review
Plant Sciences
Yi Liu, Danyu Kong, Hui-Lan Wu, Hong-Qing Ling
Summary: This article discusses the important role of iron in plant-pathogen interactions and how plants and pathogens regulate iron levels to maintain fitness. It also proposes directions for future research on this topic.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Review
Biochemistry & Molecular Biology
Timothy J. Cain, Aaron T. Smith
Summary: Iron is essential for life and its acquisition by unicellular pathogens through siderophores is crucial for virulence. Ferric reductases play a key role in reducing Fe3+ to Fe2+ for uptake, but remain poorly understood, highlighting the important connection between Fe3+ reduction and Fe2+ transport.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2021)
Article
Water Resources
D. Knabe, D. Dwivedi, H. Wang, C. Griebler, I. Engelhardt
Summary: While induced bank filtration is an effective method for sustainable drinking water production, it is susceptible to surface water contaminations. This study monitored the concentrations of adenoviruses and pathogen indicators over 16 months at an active bank filtration plant in Germany to analyze the effect of seasonal changes on pathogen transport. A groundwater model was created to simulate various factors, and the results showed that travel time and travel distance were key factors for the removal of coliforms, coliphages, and adenoviruses.
ADVANCES IN WATER RESOURCES
(2023)
Article
Immunology
Debra N. Brunson, Cristina Colomer-Winter, Ling Ning Lam, Jose A. Lemos
Summary: Bacteria often encounter restricted access to essential trace metals like iron within the host. To overcome iron deficiency, they deploy multiple strategies to scavenge iron from host tissues. Enterococcus faecalis, a major nosocomial pathogen, utilizes three conserved iron uptake systems, with two novel ABC-type transporters identified in this study.
INFECTION AND IMMUNITY
(2023)
Article
Multidisciplinary Sciences
Hiro Nakamura, Tamao Hisano, Md Mahfuzur Rahman, Takehiko Tosha, Mikako Shirouzu, Yoshitsugu Shiro
Summary: Bacterial pathogens use the ATP-binding cassette heme-dedicated efflux pump HrtBA to expel heme from the bacterial membranes to overcome cytotoxic effects.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Amber L. Grunow, Susan C. Carroll, Alicia N. Kreiman, Molly C. Sutherland
Summary: Heme trafficking is a fundamental biological process, and studying it has been challenging due to its tight regulation, cytotoxicity, and transient nature. The bacterial cytochrome c biogenesis pathways, especially System I, provide a model for understanding heme trafficking mechanisms. A detailed analysis of the WWD domain in CcmF reveals its interaction with heme and its role in attaching heme to apocytochrome c. This analysis also uncovers common mechanisms of heme interaction in different heme trafficking pathways.
Article
Microbiology
David G. Glanville, Caroline Mullineaux-Sanders, Christopher J. Corcoran, Brian T. Burger, Saheed Imam, Timothy J. Donohue, Andrew T. Ulijasz
Summary: Heme is essential for the survival of most life on earth, including bacterial pathogens like Pseudomonas aeruginosa. By using a novel approach called Met-Seq, researchers identified 188 genes that potentially influence intracellular heme levels in P. aeruginosa, shedding light on previously unknown metabolic pathways and potential therapeutic targets.
Article
Microbiology
Valentina Furlanetto, Christina Divne
Summary: In this study, the first experimental structure of a complex between Xcc LolA and LolB was reported, revealing a stable complex in the absence of LP. The structural integrity of the LP-free complex was protected by specific protein-protein interactions that were different from those predicted to be involved in lipid binding. These findings identify structural determinants for Xcc LolA to dock with LolB and initiate LP transfer.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Microbiology
Ken Ellis-Guardiola, Brendan J. Mahoney, Robert T. Clubb
Summary: This review discusses the role of NEAT domains in bacteria in scavenging iron from human hemoglobin, particularly focusing on Staphylococcus aureus. NEAT domains in S. aureus function as either Hb receptors or heme-binding chaperones, playing a crucial role in extracting heme molecules from Hb. The formation of transfer complexes among NEAT domains may facilitate the flow of heme from the microbial surface to the membrane, impacting the access of bacteria to iron from Hb.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biology
Megumi Nishinaga, Hiroshi Sugimoto, Yudai Nishitani, Seina Nagai, Satoru Nagatoishi, Norifumi Muraki, Takehiko Tosha, Kouhei Tsumoto, Shigetoshi Aono, Yoshitsugu Shiro, Hitomi Sawai
Summary: The study elucidated the structural basis of the transcription regulator PefR from Streptococcus agalactiae, a pathogen causing life-threatening neonatal infections. Structural comparison in different states (apo, DNA-bound, heme-bound, CO-heme-bound, and CN-heme-bound PefRs) revealed a unique heme coordination controlling structural rearrangement for the survival of the hemolytic bacteria causing neonatal infections.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Federico Sebastiani, Andrea Dali, Diego Javier Alonso de Armino, Lorenzo Campagni, Gaurav Patil, Maurizio Becucci, Stefan Hofbauer, Dario A. Estrin, Giulietta Smulevich
Summary: This study focuses on the carbon monoxide adducts of the wild-type and selected variants of the coproheme decarboxylase from actinobacterial Corynebacterium diphtheriae complexed with coproheme, monovinyl monopropionyl deuteroheme (MMD), and heme b. The results show that the wild-type coproheme-CO adduct is characterized by two CO conformers, hydrogen-bonded and weak polar interaction with the distal cavity. The absence of the H118 residue leads to the formation of non-H-bonded CO species. In addition, CO binding to reversed heme b and heme d is also investigated in this work.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Article
Electrochemistry
Ramsha Ijaz, Syeda Sara Hassan, Sallahuddin Panhwar, Muhammad Younis Talpur, Salah Uddin, Zubair Ahmed
Summary: This article presents a simple method for fabricating disposable electrodes to detect specific bacteria in water. The electrodes are made using green synthesized iron oxide nanoparticles and have good nanostructure and morphology, allowing for effective detection of target bacteria with a short response time. The disposable electrode exhibits a dynamic detection range, electrode stability, and selectivity, providing a new strategy for detecting bacteria in water using green nanoparticles.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2022)
Review
Biochemistry & Molecular Biology
Malte Kellermann, Felix Scharte, Michael Hensel
Summary: Pathogenic intracellular microorganisms manipulate host cells to create niches for survival and replication. This review focuses on the manipulation of organelle compartments by pathogens and highlights the specific interactions of different pathogens with host cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yuri Nagaoka, Kotone Oshiro, Yuta Yoshino, Toshiyuki Matsunaga, Satoshi Endo, Akira Ikari
Summary: This study investigated the effect of intercellular adhesion molecule CLDN1 on the anticancer drug sensitivity of small-cell lung cancer (SCLC) cells. It was found that overexpression of CLDN1 decreased the sensitivity of SCLC cells to anticancer drugs and enhanced their migratory capacity through the activation of the TGF-81/EMT signaling pathway. Treatment with EMT inhibitors showed potential in overcoming the reduced sensitivity to anticancer drugs in CLDN1-overexpressing SCLC cells.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2024)