期刊
ACS APPLIED MATERIALS & INTERFACES
卷 7, 期 32, 页码 17565-17568出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.5b04506
关键词
potassium sensors; fluorescent sensors; two-photon absorption; two-photon fluorescence microscopy; cell imaging
资金
- National Science Foundation [CBET-1517273, CHE-0832622]
- U.S. National Academy of Sciences [PGA-P210877]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1517273] Funding Source: National Science Foundation
new two-photon excited fluorescent K+ sensor is reported. The sensor comprises three moieties, a highly selective K+ chelator as the K+ recognition unit, a boron-dipyrromethene (BODIPY) derivative modified with phenylethynyl groups as the fluorophore, and two polyethylene glycol chains to afford water solubility. The sensor displays very high selectivity (>52-fold) in detecting K+ over other physiological metal cations. Upon binding K+, the sensor switches from nonfluorescent to highly fluorescent, emitting red to near-IR (NIR) fluorescence. The sensor exhibited a good two-photon absorption cross section, 500 GM at 940 nm. Moreover, it is not sensitive to pH in the physiological pH range. Time-dependent cell imaging studies via both one- and two-photon fluorescence microscopy demonstrate that the sensor is suitable for dynamic K+ sensing in living cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据