Article
Environmental Sciences
Cristian Rial-Berriel, Andrea Acosta-Dacal, Manuel Zumbado, Luis Alberto Henriquez-Hernandez, Angel Rodriguez-Hernandez, Ana Macias-Montes, Luis D. Boada, Maria Del Mar Travieso-Aja, Beatriz Martin Cruz, Octavio P. Luzardo
Summary: The study successfully adapted a blood-based detection method to liver matrix, detecting and recording dozens of different contaminants, with persistent organic pollutants being the predominant ones.
Article
Chemistry, Multidisciplinary
Ofir Schuster, Anat Zvi, Osnat Rosen, Hagit Achdout, Amir Ben-Shmuel, Ohad Shifman, Shmuel Yitzhaki, Orly Laskar, Liron Feldberg
Summary: A novel method for SARS-CoV-2 identification based on mass spectrometry was presented, which involves a multistep procedure for specific peptide marker selection and liquid chromatography coupled to high-resolution mass spectrometry. The developed assay is rapid, simple, and specific, achieving 94% concordance with the gold standard RT-qPCR method when applied to clinical samples. This MS-based assay provides a complementary approach to RT-qPCR for SARS-CoV-2 detection, showing potential for clinical applications.
Article
Environmental Sciences
Andrea Acosta-Dacal, Cristian Rial-Berriel, Ricardo Diaz-Diaz, Maria del Mar Bernal-Suarez, Manuel Zumbado, Luis Alberto Henriquez-Hernandez, Octavio P. Luzardo
Summary: A validated QuEChERS extraction method was developed for the analysis of ARs and PhACs residues in soil samples, proving to be effective for both agricultural monitoring and forensic investigations. The method was successfully validated in clay loam soil samples, with LOQ and LOD values within acceptable ranges.
Article
Engineering, Environmental
Chun-Kit Au, K. K. Jason Chan, Wan Chan, Xiangru Zhang
Summary: Through water sample analysis in rivers and coastal waters in Hong Kong, we have identified widespread PCMX pollution. Additionally, our research has demonstrated that the commonly used chemically enhanced primary treatment (CEPT) in wastewater treatment processes is not effectively removing PCMX. Furthermore, PCMX has been found to be relatively stable in water environments, posing a risk to aquatic organisms. These findings highlight the importance of increasing public awareness about the environmental impact of excessive use of PCMX-based disinfectants and the need to reevaluate wastewater treatment processes for PCMX removal.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Plant Sciences
Ashwil Klein, Lizex H. H. Husselmann, Achmat Williams, Liam Bell, Bret Cooper, Brent Ragar, David L. Tabb
Summary: Proteomics has been more valuable in model organisms with genome sequence annotations than in nonmodel organisms lacking such annotations. This study aimed to establish a protein sequence set for the nonmodel organism chia using RNA-Seq experiments. The results showed that assembling RNA-Seq datasets for chia resulted in high completeness of transcript sequences, though the number of sequences and genes varied. After six-frame translation, orthologs were detected among other species within the same taxonomic order.
Article
Chemistry, Applied
Nicolo Interino, Rossana Comito, Patrizia Simoni, Silvia Franzellitti, Giorgia Palladino, Simone Rampelli, Anastasiia Mosendz, Roberto Gotti, Aldo Roda, Marco Candela, Emanuele Porru, Jessica Fiori
Summary: This study developed and validated a liquid chromatography-mass spectrometry (HPLC-MS/MS) method for detecting pharmaceutical and pesticide residues in mussels. The method successfully detected target analytes at different concentration levels with high accuracy and low relative standard deviation for both intra-day and inter-day analyses. The method was applied to mussel samples collected from a commercial farm near the Adriatic Sea, detecting various contaminants within a certain range. The study provides a valuable tool for investigating the potential threats posed by diverse contaminant classes with high annual tonnage.
Article
Chemistry, Applied
Xuejun Xu, Jennifer Lott, Kathleen A. Kelly, Zhongping Shi
Summary: This study developed a simple and sensitive LC/MS method for controlling a diamine compound in a pharmaceutical synthetic process. It was found that the diamine significantly adsorbed to the surface of glassware, and the addition of triethylamine in the sample diluent helped mitigate the adsorption. However, at the plant scale, the adsorption of the diamine on glassware was found to be stronger than expected.
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2022)
Review
Chemistry, Analytical
Gabriel Davi Marena, Matheus Aparecido dos Santos Ramos, Tais Maria Bauab, Marlus Chorilli
Summary: This review article collects important information on the physical, chemical, and biological properties of Amphotericin B (AmB) as well as the main analytical methods for quantifying and determining it. The article provides the scientific community with important information for choosing the best analysis method and ensuring the reliability of research results.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2022)
Article
Engineering, Environmental
Qiuguo Fu, Andreas Scheidegger, Endre Laczko, Juliane Hollender
Summary: The study investigated the toxicokinetic processes and metabolomics effects of the pharmaceutical diclofenac on the freshwater invertebrate Hyalella azteca. Results showed rapid uptake and formation of diclofenac taurine, with slower elimination compared to diclofenac. Metabolomics revealed inhibition of prostaglandin synthesis and impact on the carnitine shuttle pathway at environmentally relevant concentrations.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Chiara Fania, Marco Bagnati, Marina Albertario, Carlotta Ferraris, Marta Lamonaca, Umberto Dianzani
Summary: The analysis of immunosuppressant drugs using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) is considered the gold standard. Automated systems, such as the Thermo Scientific(TM) Cascadion(TM) SM Clinical Analyzer, eliminate manual processes and provide efficient and accurate results. The comparison of LC-MS/MS with the automated system showed a good correlation between the two methods.
APPLIED SCIENCES-BASEL
(2022)
Article
Chemistry, Analytical
Linlin Dong, Nicole Bebrin, Konstantin Piatkov, Kojo Abdul-Hadi, Shinji Iwasaki, Mark G. Qian, Dong Wei
Summary: In the field of immuno-oncology, a highly sensitive and specific bioanalytical method is required for quantifying surrogate mouse antibodies, and the immunoaffinity enrichment based LC/MS/MS assay may provide an effective solution by differentiating analyte response from high endogenous immunoglobulin background.
ANALYTICAL CHEMISTRY
(2021)
Article
Toxicology
Jochem Louisse, Patrick P. J. Mulder, Arjen Gerssen, Geert Stoopen, Deborah Rijkers, Milou G. M. van de Schans, Ad A. C. M. Peijnenburg
Summary: “The study utilized a bioassay-directed analysis approach to identify potentially toxic PAs not yet included in monitoring programs. The findings suggest that this method is effective in identifying candidate PAs with genotoxic activity.”
ARCHIVES OF TOXICOLOGY
(2022)
Article
Food Science & Technology
Jing Pu, Bingye Zhao, Xin Liu, Shugang Li, Beibei Wang, Di Wu, Jinqiu Wang, Fang Geng
Summary: This study comprehensively compared the protein profiles and properties of chicken egg white and its three components. The proteomes of thick and thin egg white are similar, but thick egg white has higher abundance of ovomucin subunits and lysozymes. The properties of thick and thin egg white are significantly different. Additionally, the chalaza component has higher abundance of insoluble proteins and lower abundance of soluble proteins, which explains its insolubility. These findings are important for further research and development of egg white, such as thinning of egg white, understanding changes in egg white properties, and differential application of thick and thin egg white.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Biochemical Research Methods
Martin Meyer, Lidia Montero, Sven W. Meckelmann, Oliver J. Schmitz
Summary: This study compared the analysis of carbohydrates using four common chromatographic methods coupled to mass spectrometry. It was found that gas chromatography and reversed-phase liquid chromatography, after derivatisation, showed superior separation performance compared to the other methods. Targeted analysis using different working modes of the mass spectrometer improved sensitivity and repeatability, with RP-LC-MS in MRM mode after derivatisation being the most suitable method for monosaccharide analysis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Liron Feldberg, Anat Zvi, Yfat Yahalom-Ronen, Ofir Schuster
Summary: This study introduces a Mass Spectrometry-based method for the diagnosis of SARS-CoV-2 variants, which allows for rapid identification and variant-specific discrimination. The method is specific, easy to perform and cost-effective, making it a valuable diagnostic tool for pathogenic variants.
Article
Plant Sciences
Luca Tadini, Carlotta Peracchio, Andrea Trotta, Monica Colombo, Ilaria Mancini, Nicolaj Jeran, Alex Costa, Franco Faoro, Milena Marsoni, Candida Vannini, Eva-Mari Aro, Paolo Pesaresi
Article
Chemistry, Analytical
L. Bijlsma, R. Bade, F. Been, A. Celma, S. Castiglioni
Summary: New psychoactive substances (NPS) are emerging as legal substitutes to conventional illicit drugs, causing public health concerns. Monitoring NPS use is challenging, but determining NPS in pooled urine and wastewater shows great potential. However, this method also presents analytical challenges and limitations.
ANALYTICA CHIMICA ACTA
(2021)
Article
Plant Sciences
Annalisa Paradiso, Guido Domingo, Emanuela Blanco, Alessio Buscaglia, Stefania Fortunato, Milena Marsoni, Pasquale Scarcia, Sofia Caretto, Candida Vannini, Maria Concetta de Pinto
PLANT CELL AND ENVIRONMENT
(2020)
Article
Plant Sciences
Candida Vannini, Guido Domingo, Valentina Fiorilli, Daniel Garcia Seco, Mara Novero, Milena Marsoni, Florence Wisniewski-Dye, Marcella Bracale, Lionel Moulin, Paola Bonfante
Summary: Plants rely on their microbiota for improving nutrition and stress tolerance, with some plant growth-promoting bacteria and arbuscular mycorrhizal fungi showing potential benefits for wheat growth. Plant responses to different microbial combinations can lead to specific phenotypic and proteomic changes, influencing defense mechanisms and immune responses. The presence of arbuscular mycorrhizal fungi seems to be crucial for promoting plant growth and development in synthetic microbial communities.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Neurosciences
Guido Domingo, Luisa Benussi, Claudia Saraceno, Michela Bertuzzi, Roland Nicsanu, Antonio Longobardi, Sonia Bellini, Alfredo Cagnotto, Mario Salmona, Giuliano Binetti, Roberta Ghidoni
Summary: A newly developed antibody-free method was used to identify and quantify Aβ isoforms in human CSF, revealing the presence of novel Aβ species. Among these, five Aβ isoforms found in CSF of elderly individuals may serve as potential markers for Alzheimer's disease progression.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Plant Sciences
Francesco Venice, Matteo Chialva, Guido Domingo, Mara Novero, Andrea Carpentieri, Alessandra Salvioli di Fossalunga, Stefano Ghignone, Angela Amoresano, Candida Vannini, Luisa Lanfranco, Paola Bonfante
Summary: By analyzing Lotus japonicus roots colonized by the fungal line with bacteria (B+) and by the cured line (B-) through deep dual-mRNA sequencing and proteomics, it was found that L. japonicus activates its symbiotic pathways regardless of the presence of endobacteria, but perceives the two fungal lines as different physiological entities. Significant differences were observed in respiratory pathways and lipid biosynthesis between the mycorrhizal plants colonized by the B+ and B- lines, indicating a reduced capacity in antioxidant metabolism and symbiotic lipid biosynthesis in the cured line.
Article
Engineering, Environmental
Richard Bade, Jason M. White, Maulik Ghetia, Santosh Adiraju, Sangeet Adhikari, Lubertus Bijlsma, Tim Boogaerts, Daniel A. Burgard, Sara Castiglioni, Alberto Celma, Andrew Chappell, Adrian Covaci, Erin M. Driver, Rolf U. Halden, Felix Hernandez, Heon-Jun Lee, Alexander L. N. van Nuijs, Jeong-Eun Oh, Marco A. Pineda Castro, Noelia Salgueiro-Gonzalez, Bikram Subedi, Xue-Ting Shao, Viviane Yargeau, Ettore Zuccato, Cobus Gerber
Summary: This study focused on 28 NPS stimulants and detected 11 of them, with eutylone and 3-methylmethcathinone (3MMC) being the most commonly found and with the highest mass loads, indicating international popularity. Older generation stimulants like para-methoxyamphetamine (PMA), methylone, and mephedrone were also detected, showing the dynamic nature of the NPS market. Areas in New Zealand were found to have the highest loads of NPS stimulant consumption, demonstrating the potential public health risks associated with these substances.
ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Milena Alvarez-Vinas, Noelia Gonzalez-Ballesteros, M. Dolores Torres, Lucia Lopez-Hortas, Candida Vanini, Guido Domingo, M. Carmen Rodriguez-Arguelles, Herminia Dominguez
Summary: This study investigates the sustainable extraction and depolymerization of carrageenan from seaweed using hydrothermal treatment with subcritical water. The results show that operating at 140 degrees C yields the highest extraction rate, and the crude carrageenan can be effectively obtained through methods such as ultrafiltration and ethanol precipitation. Additionally, it is found that the crude carrageen fraction can be used to prepare gel materials with excellent mechanical properties.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Environmental Sciences
Laura Pellegrinelli, Sara Castiglioni, Clementina Cocuzza, Barbara Bertasi, Valeria Primache, Silvia Schiarea, Giulia Salmoiraghi, Andrea Franzetti, Rosario Musumeci, Michela Tilola, Elisa Galuppini, Giorgio Bertanza, Marialuisa Callegari, Fabrizio Stefani, Andrea Turolla, Emanuela Ammoni, Danilo Cereda, Elena Pariani, Sandro Binda
Summary: This study evaluated the performance of different pre-analytical and analytical methods for identifying the presence of SARS-CoV-2 in untreated municipal wastewater samples. It concluded that PEG-8000 precipitation combined with real-time RT-PCR targeting the N gene was the best workflow for detecting SARS-CoV-2 RNA in wastewater.
Article
Engineering, Environmental
Marina Celia Campos-Manas, Natan Van Wichelen, Adrian Covaci, Alexander L. N. van Nuijs, Christoph Ort, Frederic Been, Sara Castiglioni, Felix Hernandez, Lubertus Bijlsma
Summary: Wastewater analysis of THC biomarkers provides important information on cannabis consumption trends. Analyzing both the liquid and solid phases yields more accurate results.
Article
Mycology
Guido Domingo, Luca Chiodaroli, Simone Parola, Milena Marsoni, Marcella Bracale, Candida Vannini
Summary: This study focuses on the impact of log cultivation method on the production of Shiitake mushrooms and the associated bioactive compounds. Proteomic analysis revealed the main active processes and proteins involved in antioxidant and antibacterial properties. The results highlight the importance of the cultivation technique in enhancing the production of high-value bioactive compounds in Shiitake mushrooms.
Article
Biochemical Research Methods
Guido Domingo, Candida Vannini, Marcella Bracale, Paola Bonfante
Summary: The symbiotic relationship between plants and arbuscular mycorrhizal (AM) fungi leads to significant changes in plant metabolism, which are regulated by various molecular mechanisms. This study conducted a comprehensive analysis of AM-modulated proteins at both the local (roots) and systemic (shoots/leaves) levels using proteomics. The analysis identified a total of 4262 differentially abundant proteins and revealed a systemic reprogramming of translation, with a central role played by ribosomal translational apparatus.
Article
Plant Sciences
Guido Domingo, Milena Marsoni, Milena Alvarez-Vinas, M. Dolores Torres, Herminia Dominguez, Candida Vannini
Summary: The application of seaweed extract-based biostimulants shows promise in achieving sustainable agriculture by improving crop yield and mitigating the effects of climate change. In this study, a protein hydrolysate-rich C. crispus extract was tested on tomato plants and found to promote plant growth and enhance drought tolerance.
