期刊
AQUATIC TOXICOLOGY
卷 104, 期 3-4, 页码 211-217出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aquatox.2011.04.019
关键词
Brevetoxin; Neurotoxic shellfish poisoning; Oryzias melastigma; Medaka fish; Gill; Calcium ion binding
资金
- Research Grants Council of the Hong Kong Special Administrative Region, China [CityU3/CRF/08]
- State Key Laboratory of Marine Environmental Science (Xiamen University) [MEL0607]
Although brevetoxins (PbTxs) produced by the marine dinoflagellate Karenia brevis are known to be absorbed across gill membranes and exert their acute toxic effects through an ion-channel mediated pathway in neural tissue, the exact biochemical mechanism concerning PbTxs neurotoxicity in neural tissue and gas-exchange organs has not been well elucidated. In this study, we calculated the LC50 value of PbTx-1 using the medaka fish model, and presented the molecular responses of sub-acute exposure to PbTx-1 with proteomic method. By adopting two-dimensional electrophoresis, the abundances of 14 and 24 proteins were found to be remarkably altered in the gills and brains, respectively, in response to toxin exposure. Thirteen gill and twenty brain proteins were identified using matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry analysis. These proteins could be categorized into diverse functional classes such as cell structure, macromolecule metabolism, signal transduction and neurotransmitter release. These findings can help to elucidate the possible pathways by which aquatic toxins affect marine organisms within target organs. (C) 2011 Elsevier B.V. All rights reserved.
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