期刊
AQUATIC TOXICOLOGY
卷 95, 期 3, 页码 222-229出版社
ELSEVIER
DOI: 10.1016/j.aquatox.2009.09.008
关键词
Methyltestosterone; Ethynylestradiol; Endocrine disruptors; GnRH; Gonadotropins; Monoamines
资金
- Department of Biotechnology, India [BT/PR11263/AAQ/03/419/2008]
- Council of Scientific and Industrial Research, India
Adult male and female air-breathing catfish Clarias gariepinus were treated with ethynylestradiol (EE2) and methyltestosterone (MT) at concentrations of 1 mu g/L, respectively for 21 days. EE2 treatment caused disappearance of spermatids/sperm from several testicular lumen/lobules in males while MT treatment to females led to precocious ovarian development. EE2 caused significant fluid retention in all tissues including peritoneal cavity and seminal vesicles. Immunocytochemical localization of catfish GnRH (cfGnRH) and luteinizing hormone (LH) in preoptic area-hypothalamus (POA-H) and pituitary, respectively, revealed decreased immunoreactivity (ir-) following EE2 treatment in males. MT treatment however caused no observable change in cfGnRH ir- and a significant increase in LH ir- in females. Semi-quantitative RT-PCR analysis indicated that cfGnRH transcripts in POA-H decreased significantly following EE2 and MT treatment in males and females, respectively. Levels of POA-H dopamine (inhibitory monoamine for gonadotropin [GTH] synthesis and release) increased following EE2 and MT treatment in males and females while levels of serotonin and norepinephrine (GTH-stimulatory monoamines) decreased significantly. The results demonstrate a direct in vivo effect of sex steroid analogs on cfGnRH-LH axis and monoaminergic system vis-a-vis on gonads in addition to probable direct action on gonads. (C) 2009 Elsevier B.V. All rights reserved.
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