Article
Environmental Sciences
Dongju Shin, Youmi Choi, Zhi Yang Soon, Moonkoo Kim, Dae-Jung Kim, Jee-Hyun Jung
Summary: This study compared the toxic effects of CuPT and ZnPT on embryonic olive flounder and found that CuPT had a greater toxicity than ZnPT with respect to developmental malformation and mortality. Furthermore, gene expression analysis revealed that CuPT exposure affected genes associated with tail fin malformation, cellular respiration, and kidney development, while ZnPT exposure affected genes related to cell development, nervous system development, and heart development.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Fisheries
HyeongJin Roh, Bo Seong Kim, Gyoungsik Kang, Do-Hyung Kim
Summary: This study aimed to accelerate physical wound healing in flounder using LED technology, showing that 520 and 640 nm LED treatment stimulated cell proliferation, reduced mortality rate, increased skin tissue thickness, and promoted faster wound closure rates. 520 nm LED treatment is an effective and eco-friendly method for aiding recovery from physical injuries in fish, with potential application in aquaculture.
Article
Environmental Sciences
Zhen Lu, Zeyu Xiao, Sihan Wu, Jun Song, Xiao Peng
Summary: This study aims to investigate the mechanisms of Cd-induced mitochondrial toxicity. By examining the mitochondrial morphology, MMP, metabolomic and proteomic analysis in the liver of juvenile olive flounder, it was found that Cd exposure could disrupt the mitochondrial morphology, energy metabolism, and apoptosis in the liver mitochondria.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Fisheries
Kun Yang, Xueshu Zhang, Zengyu Liu, Weiqun Lu
Summary: In animals, personalities have been found to be linked to behavioral traits, health and disease susceptibility, and welfare. This study examines the performance differences of flounder offspring with different personalities (OFDP). The offspring of bold individuals (OB) were found to be heavier and have higher survival rates compared to the offspring of shy individuals (OS). The study also analyzes the variability of microsatellites and their relationship with OFDP, and finds that certain microsatellite loci are correlated with growth, heat tolerance, and disease resistance. These findings contribute to olive flounder aquaculture and provide insights for fish personality selective breeding.
Article
Food Science & Technology
D. P. Nagahawatta, N. M. Liyanage, Thilina U. Jayawardena, H. H. A. C. K. Jayawardhana, Jae-Young Oh, K. K. Asanka Sanjeewa, Sang In Kang, You-Jin Jeon
Summary: The study found that digested flounder meat has the potential to promote skeletal muscle growth and can be used as a functional food ingredient.
Article
Immunology
Qian Li, Heng Chi, Xueyan Shi, Qiujie Gan, Roy Ambli Dalmo, Yuan-yuan Sun, Xiaoqian Tang, Jing Xing, Xiuzhen Sheng, Wenbin Zhan
Summary: This study introduced a new method for detecting ETs in the peritoneum of fish in vivo and indicated that ET formation is involved in the immune response at the early stage after intraperitoneal immunization to vaccines.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Environmental Sciences
Ping Han, Yingjie Qiao, Jiayi He, Xubo Wang
Summary: This study investigated the effects of temperature rise on the liver of Japanese flounder. The results showed that heat stress can cause tissue damage, inflammation, oxidative stress, and endoplasmic reticulum stress. These findings contribute to understanding the adaptive mechanisms of economic fish in the face of increasing water temperature caused by global warming.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Genetics & Heredity
Fan Yang, Saisai Liu, Jiangbo Qu, Quanqi Zhang
Summary: The Pomstna gene in Japanese flounder has been identified as a negative regulator of muscle growth and differentiation, and it operates through a different regulatory mechanism than Pomstnb. This study suggests that muscle proliferation and differentiation are not only regulated through the TGF-beta signaling pathway, but also through other unknown mechanisms.
Article
Biology
Yucong Yang, Yuqin Ren, Yitong Zhang, Guixing Wang, Zhongwei He, Yufeng Liu, Wei Cao, Yufen Wang, Songlin Chen, Yuanshuai Fu, Jilun Hou
Summary: A new cell line, Japanese flounder spleen (JFSP), derived from the spleen of Japanese flounder (Paralichthys olivaceus), was established and characterized in this study. The JFSP cells grew rapidly at 29 degrees C, and the optimum fetal bovine serum concentration in the L-15 medium was 15%. Cells were subcultured for more than 80 passages. The JFSP cells have a diploid chromosome number of 2n = 68, which differs from the chromosome number of normal diploid Japanese flounder. The established cells were susceptible to Bohle virus (BIV), Viral hemorrhagic septicemia virus (VHSV), Hirame rhabdovirus (HIRRV), Infectious hematopoietic necrosis virus (IHNV), and Lymphocystis disease virus (LCDV), as evidenced by varying degrees of cytopathic effects (CPE). Replication of the virus in JFSP cells was confirmed by qRT-PCR and transmission electron microscopy. In addition, the expression of four immune-related genes, TRAF3, IL-1 beta, TNF-alpha, and TLR2, was differentially altered following viral infection. The results indicated that the cells underwent an antiviral immune response. JFSP cell line is an ideal tool in vitro for virology. The use of fish cell lines to study the immune genes and immune mechanism of fish and to clarify the immune mechanism of fish has important theoretical significance and practical application value for the fundamental prevention and treatment of fish diseases.
Article
Environmental Sciences
Zhengrui Zhang, Yuezhong Liu, Wei Zhang, Xinxin Du, Jinxiang Liu
Summary: The study focuses on the diversity of opsins and their expression patterns in flatfishes, particularly in Japanese flounder. The research reveals that the visual system of Japanese flounder is adapted to a benthic environment through fine-tuning paralogous opsins and regulated expression. The study suggests that opsin repertoires optimize visual function under variable light environments through gene family duplications, differential expressions, and variations in maximum absorption wavelength (lambda max).
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Fisheries
W. K. M. Omeka, D. S. Liyanage, Sukkyoung Lee, Chaehyeon Lim, Hyerim Yang, W. M. Gayashani Sandamalika, H. M. V. Udayantha, Gaeun Kim, Subothini Ganeshalingam, Taehyug Jeong, Seong-Rip Oh, Seung-Hwan Won, Hyoung-Bum Koh, Mun-Kwan Kim, David B. Jones, Cecile Massault, Dean R. Jerry, Jehee Lee
Summary: Traits linked to growth performance in farmed fish are important for profitability in aquaculture. A genome-wide association study (GWAS) on olive flounder identified several single nucleotide polymorphisms (SNPs) associated with growth traits. These findings contribute to understanding the molecular basis of growth regulation and promote the use of SNP-based genomic selection in flounder culture.
Article
Immunology
Yanbo Hao, Xiaoqian Tang, Jing Xing, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Summary: The Fc receptor plays a critical role in mediating the phagocytosis and bactericidal activity of IgM(+) B lymphocytes in flounder. It increases phagocytosis rates and affects intracellular reactive oxygen species levels. Additionally, blocking Fc receptor or Fc fragment of IgM reduces phagocytosis rates in mIgM(+) B lymphocytes.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Fisheries
Peng Zhang, Boguang Sun
Summary: This study investigates the involvement of PoCry1 in the immune response during bacterial infection in Japanese flounder. The results show that PoCry1 is involved in anti-infection immunity and exhibits circadian oscillation in transcription upon rhythmic light stimulation. These findings suggest that light condition can be exploited to strengthen fish immune system and benefit disease control in aquaculture.
Article
Fisheries
Xuangang Wang, Xiangfu Kong, Xiaobing Liu, Xubo Wang, Zhigang Wang, Jinxiang Liu, Quanqi Zhang, Haiyang Yu
Summary: Pyroptosis is a caspase-1-dependent form of programmed cell death characterized by cell lysis and inflammatory cytokine release. This study demonstrates that E. tarda infection triggers pyroptosis in macrophages of Japanese flounder, playing a vital role in the immune response. Treatment with a caspase-1 inhibitor significantly reduces pyroptosis, indicating the association of caspase-1 with pyroptosis.
Article
Multidisciplinary Sciences
Takahiro Ikari, Yukihiro Furusawa, Yoshiaki Tabuchi, Yusuke Maruyama, Atsuhiko Hattori, Yoichiro Kitani, Kenji Toyota, Arata Nagami, Jun Hirayama, Kazuki Watanabe, Atsushi Shigematsu, Muhammad Ahya Rafiuddin, Shouzo Ogiso, Keisuke Fukushi, Kohei Kuroda, Kaito Hatano, Toshio Sekiguchi, Ryotaro Kawashima, Ajai K. Srivastav, Takumi Nishiuchi, Akihiro Sakatoku, Masa-aki Yoshida, Hajime Matsubara, Nobuo Suzuki
Summary: Deep ocean water (DOW) promotes the growth of marine organisms and reduces stress in Japanese flounder. The regulation of gene expression and the activation of osteoblasts in fish scales play a role in the cortisol-reducing effect. Kynurenine, an indole component in DOW, facilitates Calcitonin production by osteoblasts and leads to a decrease in cortisol concentration.
SCIENTIFIC REPORTS
(2023)