Article
Environmental Sciences
Qin-Qin Li, Qian-Qian Xiang, Li-Hong Lian, Zhi-Ying Chen, Xia Luo, Cheng-Zhi Ding, Li-Qiang Chen
Summary: The study found that exposure to silver nanoparticles can significantly alter 96 metabolites in fish gills, primarily affecting amino acid metabolism and energy metabolism. Silver nanoparticles specifically induce changes in 72 metabolites, enriching pathways such as aminoacyl-tRNA biosynthesis, ABC transporters, and the citrate cycle. On the other hand, exposure to silver supernatant ions causes specific changes in 14 metabolites, mainly interfering with sphingolipid metabolism in fish gills.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Fisheries
Supono Supono, Xiuqing Yu, Bradley M. Skelton, William J. G. McKay, Andrew Jeffs
Summary: The nutritional condition of juvenile mussels is poorly understood, particularly regarding the impact of size and starvation. This study measured the biochemical composition of juvenile green-lipped mussels of different sizes from varying sources and subjected to different starvation periods. The findings suggest that improving carbohydrate reserves during nursery feeding regimes can enhance resilience to poor feeding conditions in coastal mussel farms.
Article
Biochemistry & Molecular Biology
Eva Klumpen, Nadine Hoffschroeer, Andrea Schwalb, Ulrike Gigengack, Marita Koch, Ruediger J. Paul, Bettina Zeis
Summary: Zooplankton organisms face variable food supply in their habitat, and metabolic adjustments during periods of starvation were analyzed in the microcrustacean Daphnia pulex, revealing the utilization of carbohydrate stores and degradation of lipid and protein reserves. Glycogenolysis and gluconeogenesis led to hyperglycaemia after 16 hours of starvation, while the concentration of alpha-ketoglutarate and oxygen consumption rate reached their maxima during this period. The inverse correlation between nuclear HIF-1 alpha levels and alpha-ketoglutarate concentration were discussed, along with the effects on prolyl hydroxylase activity and the role of this transcription factor in metabolic gene expression changes.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
T. Mace, E. Gonzalez-Garcia, G. Kover, D. Hazard, M. Taghipoor
Summary: The aim of this study was to characterize the body condition dynamics of meat sheep during their productive lifespan using a modeling approach based on body condition score measurements. The study found inter-individual variability in the rates of energy reserve accretion and mobilization, as well as the length of the mobilization period. Individual characterization of animals by these parameters can help evaluate their efficiency in utilizing body reserves during negative energy balance challenges and provide better management and decision-making for farmers and advisors, as well as reference for future animal breeding programs.
Article
Physiology
Yanqiu Zhu, Wei Xiong, Yuan Xu, Pan Zhang, Jianghui Zhang, Yiping Luo
Summary: Ploidy level affects the cell size, metabolic rate, and metabolic scaling of organisms, with triploid and diploid fish showing differences in resting metabolic rate, gill surface area, ventilation frequency, and red blood cell surface area. The larger red blood cell surface area and resting metabolic rate, as well as smaller scaling exponents of gill surface area and ventilation frequency in triploids, suggest that ploidy-induced changes in surface area and scaling affect the metabolic scaling of fish.
JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMS AND ENVIRONMENTAL PHYSIOLOGY
(2021)
Article
Chemistry, Applied
Jiawei Zhang, Yishuai Du, Yue Sun, Li Zhou, Jianping Xu, Jianming Sun, Tianlong Qiu
Summary: This study evaluated the effects of solid waste of Citrus sinensis (SWC) supplementation on common carp flesh quality and its potential mechanisms. The results showed that SWC diet significantly improved growth rate, muscle sweetness, and nutritional value of fish meat. Chromatography-mass spectrometry analysis indicated that SWC supplementation increased essential amino acid content and promoted biosynthesis of non-essential amino acids. In conclusion, SWC could provide cost-effective and nutritious aquatic products.
Article
Environmental Sciences
Jia Zhao, Hongxia Zhao, Zhihui Zhong, Tadiyose Girma Bekele, Huihui Wan, Yuming Sun, Xintong Li, Xiaonuo Zhang, Zhansheng Li
Summary: Tetrabromobisphenol A bis (allyl ether) (TBBPA-BAE) is a widely used brominated flame retardant with neurotoxic and endocrine disruption effects on aquatic organisms. This study investigated the bioconcentration, elimination kinetics, and biotransformation of TBBPA-BAE in common carp. The results showed rapid accumulation of TBBPA-BAE in the fish, with the highest concentrations found in the liver and the longest residence time in the kidney. Biotransformation of TBBPA-BAE was observed, with 14 different metabolites identified in the liver. These findings highlight the importance of considering the biotransformation products of TBBPA-BAE in risk assessment.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Umit Acar, Yavuz Erden, Burak Evren Inanan, Osman Sabri Kesbic, Sevdan Yilmaz, Ekrem Sanver Celik
Summary: Folpet, a widely used fungicide in agriculture, has toxic effects on common carp even at the lowest concentration. This study investigated the mechanism of action and potential acute toxicity of folpet in non-target aquatic organisms. The results showed that folpet exposure caused changes in hematological parameters, gene expression levels, and DNA damages in common carp.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Review
Fisheries
Clara Py, Regina Elizondo-Gonzalez, Alberto Pena-Rodriguez
Summary: Feed restriction in fish and crustaceans can lead to compensatory growth, which is the accelerated growth after a period of deprivation. This phenomenon is of interest for sustainable aquaculture development, as it allows for reduced feed consumption without loss of production. However, the physiological costs and mechanisms associated with this compensatory growth process are still not well understood.
REVIEWS IN AQUACULTURE
(2022)
Article
Marine & Freshwater Biology
Jiawen Cui, Yuhao Liu, Zhiyu Hao, Yuhang Liu, Minna Qiu, Lu Kang, Xiaohua Teng, You Tang
Summary: In this experiment, a poisoning model of common carp with cadmium (Cd) exposure was established to assess the nephrotoxicity of Cd. Results showed that Cd exposure induced kidney injury and had an impact on mitochondrial damage and apoptosis. This study provides insights into the underlying mechanisms and theoretical basis for evaluating Cd toxicity to aquatic organisms.
AQUATIC TOXICOLOGY
(2023)
Article
Multidisciplinary Sciences
Fabian Guzman-Rivas, Juan Ortega, Sergio Mora, Patricio Barria, Rodrigo Riera, Angel Urzua
Summary: The integrated biochemical condition of gonads in highly migratory marine species is closely related to reproductive success. This study compared the integrated biochemical conditions of female swordfish gonads in two different seasons and body size ranges. The results showed that the lipid contents varied significantly between seasons and body sizes, and the fatty acid profiles also varied for both seasons and body size ranges. These findings can be used as indicators of nutritional condition and health status, and can aid in fishery management models.
Article
Multidisciplinary Sciences
Qiaoling Meng, Bolei Kong, Qingxin Zeng, Cuizhi Fei, Hongliu Yu
Summary: This paper proposes a conceptual design method for a hybrid-actuated lower limb exoskeleton based on energy consumption simulation. It establishes a human-machine coupling model in OpenSim based on three proposed passive assistance schemes. The method of simulating muscle driving is used to find the scheme that can reduce the metabolic rate the most, and an active-passive cooperative control strategy is designed to improve the wearer's mobility.
Review
Endocrinology & Metabolism
Dan Du, Yugang Zhang, Canjun Zhu, Hong Chen, Jia Sun
Summary: The earliest function of hypoxia-inducible factor (HIF) was to respond to hypoxic conditions as a transcription factor. Recent studies have shown that HIF plays an essential role in central regulation of metabolism, affecting the central nervous system's response to glucose, inflammation, and hormonal influence on systemic metabolism. HIF primarily inhibits energy uptake and promotes energy expenditure in the hypothalamus, with its role depending on various factors and mechanisms within the hypothalamus.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Yanhong Li, Zhihao Zhao, Yikui Gao, Shaoxin Li, Linglin Zhou, Jie Wang, Zhong Lin Wang
Summary: This study utilizes waste materials (milk cartons) to prepare an emerging energy harvester that can convert mechanical energy into electricity, and improves energy harvesting efficiency through a charge excitation circuit. Compared to common dielectric films, waste materials can reduce costs and simplify the preparation process.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biology
Cristian-Alin Barbacariu, Cristina Mihaela Rimbu, Lenuta Dirvariu, Marian Burducea, Razvan Stefan Boiangiu, Elena Todirascu-Ciornea, Gabriela Dumitru
Summary: The aim of this study was to determine the optimal DDGS concentration in the diet of common carp. The results showed that DDGS did not significantly affect the growth and blood biochemical profile of the fish. However, it dose-dependently reduced the activity of certain antioxidant enzymes in the muscle and liver tissues. Additionally, DDGS had an impact on the microbial density in the intestine.
Article
Engineering, Environmental
Evelyn Stinckens, Lucia Vergauwen, Brett R. Blackwell, Gerald T. Anldey, Daniel L. Villeneuve, Dries Knapen
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2020)
Article
Engineering, Environmental
Dries Knapen, Evelyn Stinckens, Jenna E. Cavallin, Gerald T. Ankley, Henrik Holbech, Daniel L. Villeneuve, Lucia Vergauwen
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2020)
Editorial Material
Environmental Sciences
Dries Knapen
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
(2021)
Article
Environmental Sciences
Riccardo Massei, Dries Knapen, Adrian Covaci, Ronny Blust, Philipp Mayer, Lucia Vergauwen
Summary: The study found that sublethal effects occurred at concentrations about 5 times lower than lethal concentrations, with altered swimming activity and impaired swim bladder inflation being the most sensitive endpoints in zebrafish embryos. Most effective exposure levels fell within the range typically associated with baseline toxicity, demonstrating the importance of considering sublethal endpoints in toxicity tests.
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
(2021)
Article
Toxicology
Terje Svingen, Daniel L. Villeneuve, Dries Knapen, Eleftheria Maria Panagiotou, Monica Kam Draskau, Pauliina Damdimopoulou, Jason M. O'Brien
Summary: The AOP framework organizes scientific knowledge to infer cause-effect relationships between stressor events and toxicity outcomes, supporting chemical safety assessment and regulatory toxicology. However, developing robust AOPs requires substantial work and time, leading to a proposed more pragmatic approach for AOP development.
TOXICOLOGICAL SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Katyeny Manuela da Silva, Elias Iturrospe, Chloe Bars, Dries Knapen, Steven Van Cruchten, Adrian Covaci, Alexander L. N. van Nuijs
Summary: Metabolomics has been widely applied in toxicology, biomarker, and drug discovery, with toxicometabolomics serving as a powerful strategy to study toxic effects. The zebrafish model, being a low-cost system, shows promise in toxicometabolomics due to its highly conserved metabolic pathways.
Article
Environmental Sciences
Lisa Golz, Lisa Baumann, Pauline Pannetier, Thomas Braunbeck, Dries Knapen, Lucia Vergauwen
Summary: Thyroid hormones are involved in vertebrate eye development. Chemicals disrupting the thyroid hormone system may have severe consequences on the visual system in wildlife. A comprehensive literature review was conducted to establish an adverse outcome pathway (AOP) linking thyroid hormone system disruption to altered eye development in fish.
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
(2022)
Article
Chemistry, Analytical
Katyeny Manuela da Silva, Elias Iturrospe, Rik van den Boom, Maria van de Lavoir, Rani Robeyns, Lucia Vergauwen, Dries Knapen, Matthias Cuykx, Adrian Covaci, Alexander L. N. van Nuijs
Summary: Lipidomics analysis of zebrafish tissues using liquid chromatography-high resolution mass spectrometry provides insights into disease-related outcomes of exposure to toxic substances. Dilution of lipid extracts impacts the number of high-quality features, and retention time mapping is valuable for filtering lipid annotations.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Fisheries
Charlotte Philippe, Lucia Vergauwen, Kato Huyghe, Gudrun De Boeck, Dries Knapen
Summary: The zebrafish is an important fish model species in scientific research. Handling the fish can cause stress, impacting fish welfare and experimental outcomes. The study found that even short handling routines can induce stress responses in zebrafish, and researchers should consider this when conducting measurements or behavioral trials within an hour after handling.
JOURNAL OF FISH BIOLOGY
(2023)
Article
Environmental Sciences
Pauline Pannetier, Lisa Goelz, Maria Pissarreira Mendes T. Fagundes, Susanne Knoerr, Laura Behnstedt, Sara Coordes, Peter Matthiessen, Jane E. Morthorst, Lucia Vergauwen, Dries Knapen, Henrik Holbech, Thomas Braunbeck, Lisa Baumann
Summary: There has been increasing interest in endocrine-disrupting chemicals (EDCs) due to their wide use and the evidence of detrimental effects on humans and the environment. The aim of this project was to develop an integrated test protocol for detecting potential EDCs in fish. The integrated fish endocrine disruptor test (iFEDT) covers reproduction, early development, and sexual differentiation, allowing the identification of multiple endocrine-disruptive effects. The results of exposure tests with two well-studied EDCs, PTU and EE2, demonstrated the strong effects of these chemicals on various endocrine-related endpoints in zebrafish and their offspring.
INTEGRATED ENVIRONMENTAL ASSESSMENT AND MANAGEMENT
(2023)
Article
Environmental Sciences
Lisa Goelz, Pauline Pannetier, Teresa Fagundes, Susanne Knoerr, Laura Behnstedt, Sara Coordes, Peter Matthiessen, Jane Morthorst, Lucia Vergauwen, Dries Knapen, Henrik Holbech, Thomas Braunbeck, Lisa Baumann
Summary: This study aimed to integrate thyroid-related endpoints for fish into a test protocol and evaluate the effects of different endocrine disruptors on the thyroid system in zebrafish. The results showed that exposure to certain chemicals induced changes in thyroid follicles and eye development. The integrated Fish Endocrine Disruption Test (iFEDT) provides a promising tool for assessing the disruption of both the steroid and thyroid endocrine systems in zebrafish.
INTEGRATED ENVIRONMENTAL ASSESSMENT AND MANAGEMENT
(2023)
Article
Toxicology
Albert Braeuning, Patrick Balaguer, William Bourguet, Jordi Carreras-Puigvert, Katreece Feiertag, Jorke H. Kamstra, Dries Knapen, Dajana Lichtenstein, Philip Marx-Stoelting, Jonne Rietdijk, Kristin Schubert, Ola Spjuth, Evelyn Stinckens, Kathrin Thedieck, Rik van den Boom, Lucia Vergauwen, Martin von Bergen, Neele Wewer, Daniel Zalko
Summary: In the past, the focus of analyzing the endocrine disrupting properties of chemicals was mainly on estrogenic or androgenic properties, as well as steroidogenesis and thyroid signaling. However, more recent attention has been given to the disruption of energy metabolism and related signaling pathways by exogenous substances known as metabolism-disrupting chemicals (MDCs). This paper provides an overview of the efforts within the EU-funded Partnership for the Assessment of Risks of Chemicals (PARC) to develop novel in vitro methods for detecting endocrine metabolic disruptors and improving the toxicological toolbox.
FRONTIERS IN TOXICOLOGY
(2023)
Review
Toxicology
Louise Ramhoj, Marta Axelstad, Yoni Baert, Ana I. Canas-Portilla, Frederic Chalmel, Lars Dahmen, Antonio De La Vieja, Bertrand Evrard, Ann-Cathrin Haigis, Timo Hamers, Kim Heikamp, Henrik Holbech, Patricia Iglesias-Hernandez, Dries Knapen, Lorna Marchandise, Jane E. Morthorst, Nikolai Georgiev Nikolov, Ana C. V. E. Nissen, Michael Oelgeschlaeger, Kostja Renko, Vera Rogiers, Gerrit Schueuermann, Evelyn Stinckens, Mette H. Stub, Monica Torres-Ruiz, Majorie Van Duursen, Tamara Vanhaecke, Lucia Vergauwen, Eva Bay Wedebye, Terje Svingen
Summary: Current test strategies for identifying thyroid hormone (TH) system disruptors are insufficient for robust chemical risk assessment, and alternative test methods are needed to infer a causal relationship between initial chemical disruption and adverse outcomes. This project aims to develop specific new approaches to TH system disruption by characterizing in vivo mechanisms of action and incorporating non-mammalian vertebrate test species alongside traditional laboratory rat species and human-derived in vitro assays.
FRONTIERS IN TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Rik van den Boom, Lucia Vergauwen, Noortje Koedijk, Katyeny Manuela da Silva, Adrian Covaci, Dries Knapen
Summary: This study investigates the response of PON1 to metabolic changes and oxidative stress caused by a western diet, as well as the impact of BPA exposure on these responses. The findings suggest that a combination of western diet and BPA exposure amplifies the metabolic shift and oxidative stress, leading to an increase in PON1 activity.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Article
Toxicology
Karine Audouze, Elias Zgheib, Khaled Abass, Asma H. Baig, Isabel Forner-Piquer, Henrik Holbech, Dries Knapen, Pim E. G. Leonards, Diana I. Lupu, Saranya Palaniswamy, Arja Rautio, Maria Sapounidou, Olwenn V. Martin
FRONTIERS IN TOXICOLOGY
(2021)