期刊
AQUATIC MICROBIAL ECOLOGY
卷 54, 期 2, 页码 163-170出版社
INTER-RESEARCH
DOI: 10.3354/ame01266
关键词
Cyanophage; Planktothrix agardhii; Viral infection; Blooms; Cyanobacteria; Freshwater lake
资金
- National Major Basic Research Program [2004CB117403]
- National 863 High Technology Research Foundation of China
- National Natural Science Foundation of China [30671616, U0631008]
A previously unknown cyanophage, PaV-LD (Planktothrix agardhii Virus isolated from Lake Donghu), which causes lysis of the bloom-forming filamentous cyanobacterium P. agardhii, was isolated from Lake Donghu, Wuhan, China. PaV-LD only lysed P. agardhii strains isolated from Lake Donghu and not those isolated from other lakes. The PaV-LD particle has an icosahedral, non-tailed structure, ca. 70 to 85 nm (mean +/- SD = 76 +/- 6 nm) in diameter. PaV-LD was stable at freezing temperature, but lost its infectivity at temperatures >50 degrees C. Lysis of host cells was delayed about 3 d after the PaV-LD treatment with chloroform, and the virus was inactivated by exposure to low pH (<= 4). The latent period and burst size of the PaV-LD were estimated to be 48 to 72 h and about 340 infectious units per cell, respectively. The regrowth cultures of surviving host filaments were not lysed by the PaV-LD suspension. To our knowledge, this is the first isolation and cultivation of a virus infectious to the filamentous bloom-forming cyanobacterium Planktothrix from a freshwater lake.
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