Article
Agriculture, Dairy & Animal Science
Huiping Yang, Yuanzi Huo, Jayme C. Yee, Scott Rikard, William C. Walton, Eric Saillant
Summary: The study focused on the establishment of a sperm repository for the Eastern oyster Crassostrea virginica to support breeding programs in the Gulf of Mexico. Fresh sperm samples were collected from wild populations along the U.S. Gulf of Mexico coast and cryopreserved for future use. The streamlined process demonstrated in the study can serve as a template for constructing similar oyster germplasm repositories.
Article
Fisheries
Albert Kjartan Dagbjartarson Imsland, Emily Purvis, Helena C. Reinardy, Lauri Kapari, Ellie Jane Watts, Thor Arne Hangstad
Summary: The study compared the use of fresh and cryogenically frozen lumpfish sperm for fertilization, with fresh sperm resulting in higher fertilization and survival rates for eggs, although there was no significant difference in the weight of the larvae.
Article
Fisheries
Sen Yang, Bin Fan, Xinghan Chen, Yanglin Hua, Zining Meng
Summary: This study aimed to develop an efficient and standardized cryopreservation protocol for giant grouper semen. The results showed that using 10% DMSO as cryoprotectant and glucose-based extender ELRS3 at freezing height 5-7 cm produced higher post-thaw sperm motility. The optimal sperm concentration in straws ranged from 2 to 8 x 10(9) spermatozoa/mL.
Article
Fisheries
Lionel Degremont, Elise Maurouard, Antoine Jourdan, Jean-Baptiste Lamy, Pierre Boudry
Summary: The study found simultaneous hermaphrodite individuals in Pacific oysters, and demonstrated the feasibility of producing inbred lines through two successive generations of self-fertilization. The observed inbreeding coefficient of the offspring was lower than expected, suggesting better survival of heterozygous offspring due to inbreeding depression. This study highlights the potential for higher heterozygosity in inbred lines of Pacific oysters produced by self-fertilization.
Article
Obstetrics & Gynecology
Jonathan Lewin, Tomasz Lukaszewski, Phillippa Sangster, Elizabeth Williamson, Kevin McEleny, Bassel H. Al Wattar, Ephia Yasmin
Summary: This study aimed to compare the reproductive outcomes of ICSI with SSR using fresh and cryopreserved sperm and with the use of epididymal and testicular sperm. The results showed that cycles using epididymal sperm had higher live birth rates, pregnancy rates, and implantation rates compared to cycles using testicular sperm. However, there were no significant differences in outcomes between cycles using fresh or cryopreserved sperm for SSR-ICSI.
FERTILITY AND STERILITY
(2023)
Article
Cell Biology
Shaharior Hossen, Zahid Parvez Sukhan, Yusin Cho, Cheol Young Choi, Kang Hee Kho
Summary: The present study investigated the effects of saccharides and vitamins on the cryopreservation of Pacific abalone sperm. The study found that cryopreservation using saccharides improved post-thaw sperm quality, while vitamins did not have a significant impact. Specifically, the use of 3% sucrose and 8% dimethyl sulfoxide resulted in better sperm quality.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Fisheries
Ingrid Lein, Adriana Neeltje de Vries, Helge Tveiten, Terje Refstie, Bjarne Gjerde
Summary: This study demonstrates that lumpfish sperm can be stored at 3-4 degrees Celsius for up to 14 days and can be successfully cryopreserved and thawed without a significant reduction in fertilization success.
Article
Fisheries
Irfan Zidni, Jung Yeol Park, Yun Ho Lee, Hyo Bin Lee, Han Kyu Lim
Summary: This study evaluated the effects of long-term storage on cryopreserved sperm of the giant grouper and found that 1-year storage resulted in the highest post-thaw sperm motility. The cell survival rate after 1 year was similar to fresh sperm, but the rate of DNA damage was higher. Fertility and hatching rates were higher after 1 and 3 years of storage, but decreased with longer storage duration.
Article
Environmental Sciences
Shuhao Xu, Weijun Wang, Bingqing Xu, Xin He, Zhixu Chen, Xiaoran Zhao, Yifei Zhang, Shuyu Zhou, Yan Fang, Qing Wang
Summary: This study investigated the effects of environmentally relevant concentrations of the progestin norgestrel (NGT) on the sperm of Pacific oysters. The results showed that NGT had a positive effect on sperm motility, but also caused oxidative stress and decreased fertilization rates. However, hatching rates were not significantly affected.
MARINE ENVIRONMENTAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Le Chang, Chengpeng Lu, Junquan Zhu, Yiner Chen, Chunlin Wang, Changkao Mu, Congcong Hou
Summary: Portunus trituberculatus, an important marine economic species, has a rapidly developing aquaculture industry. However, wild capture and germplasm degradation have become a serious issue, calling for the development of artificial farming and germplasm resource protection. Sperm cryopreservation technology is an effective method, and this study compared three methods for obtaining free sperm, finding that mesh-rubbing is the best. Optimal cryopreservation conditions were determined, including sterile calcium-free artificial seawater as the medium, 20% glycerol as the cryoprotectant, and specific cooling and thawing procedures. However, the expression of sperm-related genes and enzymatic activities significantly decreased after cryopreservation, indicating damage to the sperm. Overall, this research improves the cryopreservation technology for P. trituberculatus sperm and provides a technical basis for establishing a cryopreservation library of crustaceans.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Hanne De Kort, Sylvain Legrand, Olivier Honnay, James Buckley
Summary: This study explores the effect of transposable elements on genome-wide heterozygosity and selection signatures across an inbreeding gradient in Arabidopsis lyrata. The results show that intense inbreeding is associated with elevated heterozygosity downstream of several transposable element superfamilies, as well as signatures of balancing selection. In addition, stress-responsive genes downstream of transposable elements also exhibit increased heterozygosity. The study also reveals specific selection signatures associated with transposable element superfamilies, which are reproducible in independent evolutionary lineages of A. lyrata. These findings provide an important hypothesis for the success of self-fertilizing species.
NATURE COMMUNICATIONS
(2022)
Article
Agriculture, Dairy & Animal Science
Thales de Souza Franca, Itamar Cossina Gomes, Eduardo Antonio Sanches, Maritza Perez Atehortua, Nathalia Santos Teixeira, Romulo Batista Rodrigues, Thaiza Rodrigues de Freitas, Andrea Giannotti Galuppo, Monike Quirino, Jhony Lisboa Benato, Thales Lysakowski Flores Machado, Lis Santos Marques, Ivan Cunha Bustamante-Filho, Fernando Pandolfo Bortolozzo, Danilo Pedro Streit Jr
Summary: The study evaluated the effect of post-thaw dilution of Rhamdia quelen sperm on its quality and reproductive capacity. It found that post-thaw dilution improved sperm velocity, straightness, progression, and beat cross frequency. However, there were no differences in membrane integrity, mitochondrial activity, and normal larvae rate. Fertilization and hatching rates were higher in the post-thaw diluted samples compared to the control samples. Therefore, this dilution method can be included in the sperm cryopreservation protocol for R. quelen.
ANIMAL REPRODUCTION SCIENCE
(2022)
Article
Multidisciplinary Sciences
Zoe G. Nichols, Scott Rikard, Sayyed Mohammad Hadi Alavi, William C. Walton, Ian A. E. Butts
Summary: The study revealed that sperm motility in Eastern oyster is regulated by extracellular K+, Ca2+, and Na+ ions, and salinity and pH levels also impact sperm activity and velocity. These findings not only contribute to understanding the physiological mechanisms in Eastern oyster, but also provide insights for artificial reproduction and sperm storage protocols.
Article
Fisheries
Bernadett Pataki, Akos Horvath, Gergely Meszaros, Nevena Kitanovic, Andras Acs, Arpad Hegyi, Jozsef Molnar, Balazs Csorbai, Bela Urbanyi
Summary: Absorbance and computer-assisted sperm analysis (CASA) were used to assess the sperm concentration of common carp, and the effect of different concentrations on post-thaw sperm parameters and fertilizing capacity were tested. The results showed that both spectrophotometry and CASA are suitable for assessing common carp sperm concentration, and maximizing sperm concentration before cryopreservation results in higher fertilization.
AQUACULTURE REPORTS
(2022)
Article
Environmental Sciences
Shaharior Hossen, Zahid Parvez Sukhan, Yusin Cho, Kang Hee Kho
Summary: The study focused on the effects of cryopreservation on gene expression and post-thaw sperm quality of Pacific abalone, suggesting that 8% Me2SO is the most suitable cryoprotectant for preserving sperm quality and DNA integrity.
FRONTIERS IN MARINE SCIENCE
(2021)
Review
Fisheries
Yangqing Zeng, Huiping Yang
Summary: The text provides a review of condition index (CI) calculations in molluscan bivalves, evaluating the CI equations for northern quahogs and identifying several interchangeable and effective equations.
AQUACULTURE RESEARCH
(2021)
Article
Fisheries
Erangi J. Heekenda, James D. Austin, Zhe Zhang, Huiping Yang
JOURNAL OF SHELLFISH RESEARCH
(2020)
Article
Fisheries
Michael P. Acquafredda, Ximing Guo, Daphne Munroe
Summary: Selective breeding can enhance heat tolerance in cultivated bivalves vulnerable to heat stress, as shown by the study on adult farmed Atlantic surfclams. The research found that exposure to high temperatures during the juvenile stage led to improved heat tolerance in adult survivors, and this trait could be passed on to subsequent generations through selective breeding. Additionally, the growth of heat-selected progeny was similar to control Atlantic surfclams, indicating the potential of selective breeding as a strategy to enhance survival in bivalve aquaculture facing climate change challenges.
NORTH AMERICAN JOURNAL OF AQUACULTURE
(2021)
Article
Biology
Carolina Penaloza, Alejandro P. Gutierrez, Lel Eory, Shan Wang, Ximing Guo, Alan L. Archibald, Tim P. Bean, Ross D. Houston
Summary: A chromosome-level assembly for the Pacific oyster (C. gigas) was created and annotated, providing a valuable resource for genetics and genomics studies, as well as selective breeding in aquaculture.
Review
Fisheries
Huiping Yang, Yuanzi Huo, Jayme C. Yee, Charles Yarish
Summary: The expansion of global macroalgal aquaculture and climate change have highlighted the importance of germplasm preservation in the context of aquaculture strains and natural biodiversity. While there have been limited studies on macroalgal germplasm cryopreservation compared to fish and shellfish species, various approaches such as programmed controlled cooling and vitrification have shown effectiveness in preserving macroalgal samples. The variability in post-thaw viability in different studies underscores the need for continued research and improvement in the field of macroalgal germplasm cryopreservation.
Article
Fisheries
Yuanzi Huo, Jayme C. Yee, Huiping Yang
Summary: The study aimed to develop a cost-effective and portable freezing system for germplasm cryopreservation of fish and shellfish. Utilizing an aeration system, different cooling rates were achieved and had an impact on post-thaw sperm motility.
AQUACULTURE RESEARCH
(2022)
Article
Biology
Huiping Yang, E. Hu, Jennifer L. Matthews, Zoltan M. Varga, Terrence R. Tiersch
Summary: The goal of this study was to investigate the efficiency of supplementing cryoprotective medium with catalase (CAT) for zebrafish sperm cryopreservation. The study found that CAT provided some protection against oxidative stress before freezing, but did not have any effect after thawing.
Article
Fisheries
Yangqing Zeng, Yuanzi Huo, Huiping Yang
Summary: This study characterized the basic immunological assays of hemocytes in the northern quahog using flow cytometry, focusing on non-lethal hemolymph collection, hemocyte count and type identification, viability assay based on plasma membrane integrity, and phagocytosis assay with fluorescence beads. The study revealed the basic characteristics of hemolymph in northern quahogs and provided methodologies for evaluating hemocyte responses to environmental stresses in clam aquaculture.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Dan Zeng, Ximing Guo
Summary: This study investigates the growth of the eastern oyster Crassostrea virginica at molecular levels by sequencing and comparing the mantle transcriptomes of large and small oysters. The results reveal differentially expressed genes related to shell formation and growth, and identify novel candidate genes and markers associated with these processes. The study also suggests the importance of both transcriptional modulation and functional polymorphism in determining growth.
MARINE BIOTECHNOLOGY
(2022)
Article
Genetics & Heredity
Jiulin Chan, Lu Wang, Li Li, Kang Mu, David Bushek, Yue Xu, Ximing Guo, Guofan Zhang, Linlin Zhang
Summary: Infectious disease outbreaks are causing declines in marine invertebrates, with the Eastern oyster being susceptible to Dermo while the Pacific oyster is resistant due to differences in their immune responses. Transcriptomic responses to the parasite showed differences in immune gene families and potential coevolution between the two oyster species.
FRONTIERS IN GENETICS
(2021)
Article
Agriculture, Dairy & Animal Science
Huiping Yang, Yuanzi Huo, Jayme C. Yee, Scott Rikard, William C. Walton, Eric Saillant
Summary: The study focused on the establishment of a sperm repository for the Eastern oyster Crassostrea virginica to support breeding programs in the Gulf of Mexico. Fresh sperm samples were collected from wild populations along the U.S. Gulf of Mexico coast and cryopreserved for future use. The streamlined process demonstrated in the study can serve as a template for constructing similar oyster germplasm repositories.
Article
Biology
Ao Li, He Dai, Ximing Guo, Ziyan Zhang, Kexin Zhang, Chaogang Wang, Xinxing Wang, Wei Wang, Hongju Chen, Xumin Li, Hongkun Zheng, Li Li, Guofan Zhang
Summary: Understanding the roles of genetic divergence and phenotypic plasticity in adaptation is crucial in evolutionary biology. Analysis of estuarine oysters revealed low genomic diversity and population structures shaped by historical factors, with genes responding to temperature and salinity stress showing strong selection signals. Gene expansion and selection enhance phenotypic plasticity critical for adapting to rapidly changing environments.
COMMUNICATIONS BIOLOGY
(2021)
Article
Fisheries
Tao Wang, Xuexi Wang, Akram Ismael Shehata, Ruoxuan Wang, Huiping Yang, Yilei Wang, Jianfeng Wang, Ziping Zhang
Summary: This study evaluated the effects of replacing fish meal with yellow mealworm (YM) and black soldier fly larvae (BSFL) on the growth performance and antioxidant capacity of red claw crayfish. The results showed that crayfish fed YM18 and BSFL14 had the highest weight gain and the lowest feed conversion ratio. The hepatopancreas of these crayfish also exhibited higher antioxidant enzyme activity. Crayfish fed BSFL14 showed higher antioxidant capacity and lower oxidative lipid content in hemocytes compared to the control group. Additionally, the expression of certain genes related to antioxidant capacity was upregulated in the hepatopancreas of crayfish. These findings suggest that appropriate use of YM and BSFL as fish meal replacements can improve the growth performance and antioxidant capacity of red claw crayfish.
AQUACULTURE RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Jingwei Song, James D. Austin, Huiping Yang
Summary: This study compared the molecular responses of the northern quahog and its southern congener to prolonged heat stress. The southern quahog was found to be more sensitive to oxidative stress and protein degradation pathways, indicating its potential vulnerability to continued ocean warming.
MARINE BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Ximing Guo, Jonathan B. Puritz, Zhenwei Wang, Dina Proestou, Standish Allen, Jessica Small, Klara Verbyla, Honggang Zhao, Jaime Haggard, Noah Chriss, Dan Zeng, Kathryn Lundgren, Bassem Allam, David Bushek, Marta Gomez-Chiarri, Matthew Hare, Christopher Hollenbeck, Jerome La Peyre, Ming Liu, Katie E. Lotterhos, Louis Plough, Paul Rawson, Scott Rikard, Eric Saillant, Robin Varney, Gary Wikfors, Ami Wilbur
Summary: The Eastern Oyster Breeding Consortium has developed two SNP arrays for the eastern oyster, enabling efficient genotyping and selection. These arrays provide easy identification and separation of selected stocks and can also be used for pedigree monitoring, assessment of oyster restoration success, and genetic improvement. The arrays will advance genomic research and accelerate the breeding of eastern oysters.
MARINE BIOTECHNOLOGY
(2023)
