期刊
AQUACULTURE RESEARCH
卷 46, 期 1, 页码 131-140出版社
WILEY-BLACKWELL
DOI: 10.1111/are.12167
关键词
Epinephelus coioides; larvae; bacterial community; DGGE
类别
资金
- National Natural Science Foundation of China [30600461]
- Science and Technology Project for Outstanding Young Scholarship of Fujian Provincial Department of Education [JA10183]
- Xiamen Key Laboratory Project [3502Z20120013]
- Foundation of Shang-Da Li in Jimei University [C511016]
- Foundation for Innovative Research Team of Jimei University [2011A001]
The gut microbiota plays key roles in the health and general welfare of fish larvae, the present study characterized the bacterial communities associated with grouper Epinephelus coioides larvae during a period of 22days post hatch (DPH) in an intensive hatchery using both cultivation-based and cultivation-independent approaches. Both approaches confirmed that bacteria were present in the gut of larvae before and after the onset of exogenous feeding, and the number of cultiviable bacteria increased gradually from 2 DPH to 22 DPH. A more complex bacterial profile was present in larvae fed fertilizer oyster eggs for 4days (8 DPH), probably as a result of the onset of exogenous feeding. Interestingly, similar internal microbiota were observed in larvae fed fertilized oyster eggs for 4days (8 DPH) and rotifers for 2weeks (22 DPH), although different microbial communities were present in the two feeds. This might suggest that the gut environment of E. coioides larvae selects for a common microbiota, which is more closely related with the rearing water than the two feeds. Therefore, bacterial community of the rearing water may play a critical role in the establishment of gut microbiota of fish larvae and more attention should be paid to its practical modulation by using probiotics. In addition, some potentially beneficial bacteria, such as Lactococcus spp., were the major components of the microbiota associated with fertilized oyster eggs, while these bacteria were not detected in larvae samples.
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