4.5 Article

Expression of recombinant phytase of Bacillus subtilis E20 in Escherichia coli HMS 174 and improving the growth performance of white shrimp, Litopenaeus vannamei, juveniles by using phytase-pretreated soybean meal-containing diet

期刊

AQUACULTURE NUTRITION
卷 19, 期 2, 页码 117-127

出版社

WILEY-BLACKWELL
DOI: 10.1111/j.1365-2095.2012.00946.x

关键词

Bacillus subtilis E20; growth performance; Litopenaeus vannamei; phytase; soybean meal

资金

  1. National Science Council, Taiwan [NSC 98-2221-E-020-013, NSC 98-2622-B-020-007-CC3]

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To produce a thermostable and neutral phytase (phy) of Bacillus subtilis E20 in Escherichia coli HMS174 and evaluate its efficiency in improving growth performance. The phy C of B.subtilis E20 was expressed in E.coli HMS 174, and then the 42-kDa recombinant phy C was purified by Ni-NAT and analysed by SDSPAGE. The recombinant phy C had optimal ranges of pH of 6 similar to 7 and temperature of 50 similar to 60 degrees C. A thermostability analysis showed that the enzyme is a thermostable phytase, and around 33% of residual activity was detected after being incubated at 90 similar to 100 degrees C for 10min. The recombinant phy C-pretreated soybean meal for feed preparation improved white shrimp, Litopenaeus vannamei, growth and feed efficiency. Overall, the neutral and thermostable phy C is suitable for aquafeed, and it is able to improve the nutritional utilization, resulting in enhanced shrimp growth and reduced feed costs.

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